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Q4QHI7 (Q4QHI7_LEIMA) Unreviewed, UniProtKB/TrEMBL

Last modified April 16, 2014. Version 60. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
Isocitrate dehydrogenase [NADP] PIRNR PIRNR000108

EC=1.1.1.42 PIRNR PIRNR000108
Gene names
ORF Names:LMJF_10_0290 EMBL CAJ02471.1
OrganismLeishmania major [Reference proteome]
Taxonomic identifier5664 [NCBI]
Taxonomic lineageEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeLeishmaniinaeLeishmania

Protein attributes

Sequence length435 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH. PIRNR PIRNR000108

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity. PIRNR PIRNR000108 PIRSR PIRSR000108-3

Sequence similarities

Belongs to the isocitrate and isopropylmalate dehydrogenases family. PIRNR PIRNR000108 RuleBase RU004443

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Nucleotide binding101 – 1033NADP By similarity
Nucleotide binding334 – 3396NADP By similarity
Region120 – 1267Substrate binding By similarity PIRSR PIRSR000108-2

Sites

Metal binding2761Magnesium or manganese By similarity PIRSR PIRSR000108-3
Metal binding2991Magnesium or manganese By similarity PIRSR PIRSR000108-3
Binding site1031Substrate By similarity PIRSR PIRSR000108-2
Binding site1081NADP By similarity
Binding site1351Substrate By similarity PIRSR PIRSR000108-2
Binding site1581Substrate By similarity PIRSR PIRSR000108-2
Binding site2841NADP By similarity
Binding site3521NADP; via amide nitrogen and carbonyl oxygen By similarity
Site1651Critical for catalysis By similarity PIRSR PIRSR000108-1
Site2361Critical for catalysis By similarity PIRSR PIRSR000108-1

Sequences

Sequence LengthMass (Da)Tools
Q4QHI7 [UniParc].

Last modified July 19, 2005. Version 1.
Checksum: 685D1D34CB6A5CC3

FASTA43548,490
        10         20         30         40         50         60 
MFRHVSAASA SSLVAARSFS STNVYLDKRI KVKNEVVDMD GDEMTRIIWS FIKEKLILPY 

        70         80         90        100        110        120 
VDVPINYFDL SVTNRDATND KVTVEAAEAV KKCNVGIKCA TITPDEARVK EFNLKKMWKS 

       130        140        150        160        170        180 
PNGTIRNILG GTVFREPIIV SNIPRIVPQW HNPIVVGRHA FGDQYKATDA VLKPGKLQLV 

       190        200        210        220        230        240 
HTPADGSAPT TLDVYDFKGE GVGLAMYNTK ESIEGFAKSC FQYALMRKYP LVLTTKNTIL 

       250        260        270        280        290        300 
KKYDGMFLQT FQRMYDEQYK ADFEKAGITY NHRLIDDQVA QMIKGEGGFV WACKNYDGDV 

       310        320        330        340        350        360 
QSDIVAQGFG SLGLMTSVLM CPDGKTIEAE AAHGTVTRHY RQHQQGKETS TNSVASIYAW 

       370        380        390        400        410        420 
TRGLAHRGKL DGNSDLVKFS ETLERVVVKA IEDGHMTKDL ALCVYGSSGV KREHYETTEQ 

       430 
FLDSVDTALK KAMSA 

« Hide

References

[1]"The genome of the kinetoplastid parasite, Leishmania major."
Ivens A.C., Peacock C.S., Worthey E.A., Murphy L., Aggarwal G., Berriman M., Sisk E., Rajandream M.A., Adlem E., Aert R., Anupama A., Apostolou Z., Attipoe P., Bason N., Bauser C., Beck A., Beverley S.M., Bianchettin G. expand/collapse author list , Borzym K., Bothe G., Bruschi C.V., Collins M., Cadag E., Ciarloni L., Clayton C., Coulson R.M.R., Cronin A., Cruz A.K., Davies R.M., De Gaudenzi J., Dobson D.E., Duesterhoeft A., Fazelina G., Fosker N., Frasch A.C., Fraser A., Fuchs M., Gabel C., Goble A., Goffeau A., Harris D., Hertz-Fowler C., Hilbert H., Horn D., Huang Y., Klages S., Knights A., Kube M., Larke N., Litvin L., Lord A., Louie T., Marra M., Masuy D., Matthews K., Michaeli S., Mottram J.C., Mueller-Auer S., Munden H., Nelson S., Norbertczak H., Oliver K., O'neil S., Pentony M., Pohl T.M., Price C., Purnelle B., Quail M.A., Rabbinowitsch E., Reinhardt R., Rieger M., Rinta J., Robben J., Robertson L., Ruiz J.C., Rutter S., Saunders D., Schaefer M., Schein J., Schwartz D.C., Seeger K., Seyler A., Sharp S., Shin H., Sivam D., Squares R., Squares S., Tosato V., Vogt C., Volckaert G., Wambutt R., Warren T., Wedler H., Woodward J., Zhou S., Zimmermann W., Smith D.F., Blackwell J.M., Stuart K.D., Barrell B.G., Myler P.J.
Science 309:436-442(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MHOM/IL/81/Friedlin.
[2]"Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania."
Rogers M.B., Hilley J.D., Dickens N.J., Wilkes J., Bates P.A., Depledge D.P., Harris D., Her Y., Herzyk P., Imamura H., Otto T.D., Sanders M., Seeger K., Dujardin J.C., Berriman M., Smith D.F., Hertz-Fowler C., Mottram J.C.
Genome Res. 21:2129-2142(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Friedlin EMBL CAJ02471.1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
FR796406 Genomic DNA. Translation: CAJ02471.1.
RefSeqXP_001681361.1. XM_001681309.1.

3D structure databases

ProteinModelPortalQ4QHI7.
SMRQ4QHI7. Positions 27-433.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsLmjF.10.0290:mRNA; LmjF.10.0290:pep; LmjF.10.0290.
GeneID5649629.
KEGGlma:LMJF_10_0290.

Phylogenomic databases

HOGENOMHOG000019858.
KOK00031.
OMAQHQQGKP.
ProtClustDBPTZ00435.

Family and domain databases

Gene3D3.40.718.10. 1 hit.
InterProIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERPTHR11822. PTHR11822. 1 hit.
PfamPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFPIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsTIGR00127. nadp_idh_euk. 1 hit.
PROSITEPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameQ4QHI7_LEIMA
AccessionPrimary (citable) accession number: Q4QHI7
Entry history
Integrated into UniProtKB/TrEMBL: July 19, 2005
Last sequence update: July 19, 2005
Last modified: April 16, 2014
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)