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Q4QHI7

- Q4QHI7_LEIMA

UniProt

Q4QHI7 - Q4QHI7_LEIMA

Protein

Isocitrate dehydrogenase [NADP]

Gene

LMJF_10_0290

Organism
Leishmania major
Status
Unreviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 64 (01 Oct 2014)
      Sequence version 1 (19 Jul 2005)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.UniRule annotation

    Cofactori

    Binds 1 magnesium or manganese ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei103 – 1031SubstrateUniRule annotation
    Binding sitei108 – 1081NADPUniRule annotation
    Binding sitei135 – 1351SubstrateUniRule annotation
    Binding sitei158 – 1581SubstrateUniRule annotation
    Sitei165 – 1651Critical for catalysisUniRule annotation
    Sitei236 – 2361Critical for catalysisUniRule annotation
    Metal bindingi276 – 2761Magnesium or manganeseUniRule annotation
    Binding sitei284 – 2841NADPUniRule annotation
    Metal bindingi299 – 2991Magnesium or manganeseUniRule annotation
    Binding sitei352 – 3521NADP; via amide nitrogen and carbonyl oxygenUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi101 – 1033NADPUniRule annotation
    Nucleotide bindingi334 – 3396NADPUniRule annotation

    GO - Molecular functioni

    1. isocitrate dehydrogenase (NADP+) activity Source: UniProtKB-EC
    2. magnesium ion binding Source: InterPro
    3. NAD binding Source: InterPro

    GO - Biological processi

    1. isocitrate metabolic process Source: InterPro
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    OxidoreductaseUniRule annotationImported

    Keywords - Biological processi

    Tricarboxylic acid cycleUniRule annotation

    Keywords - Ligandi

    MagnesiumUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotation, NADPUniRule annotation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isocitrate dehydrogenase [NADP]UniRule annotation (EC:1.1.1.42UniRule annotation)
    Gene namesi
    ORF Names:LMJF_10_0290Imported
    OrganismiLeishmania majorImported
    Taxonomic identifieri5664 [NCBI]
    Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeLeishmaniinaeLeishmania
    ProteomesiUP000000542: Chromosome 10

    Structurei

    3D structure databases

    ProteinModelPortaliQ4QHI7.
    SMRiQ4QHI7. Positions 27-433.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni120 – 1267Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the isocitrate and isopropylmalate dehydrogenases family.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000019858.
    KOiK00031.
    OMAiKNTIMKV.

    Family and domain databases

    Gene3Di3.40.718.10. 1 hit.
    InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR004790. Isocitrate_DH_NADP.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view]
    PANTHERiPTHR11822. PTHR11822. 1 hit.
    PfamiPF00180. Iso_dh. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000108. IDH_NADP. 1 hit.
    TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
    PROSITEiPS00470. IDH_IMDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q4QHI7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFRHVSAASA SSLVAARSFS STNVYLDKRI KVKNEVVDMD GDEMTRIIWS    50
    FIKEKLILPY VDVPINYFDL SVTNRDATND KVTVEAAEAV KKCNVGIKCA 100
    TITPDEARVK EFNLKKMWKS PNGTIRNILG GTVFREPIIV SNIPRIVPQW 150
    HNPIVVGRHA FGDQYKATDA VLKPGKLQLV HTPADGSAPT TLDVYDFKGE 200
    GVGLAMYNTK ESIEGFAKSC FQYALMRKYP LVLTTKNTIL KKYDGMFLQT 250
    FQRMYDEQYK ADFEKAGITY NHRLIDDQVA QMIKGEGGFV WACKNYDGDV 300
    QSDIVAQGFG SLGLMTSVLM CPDGKTIEAE AAHGTVTRHY RQHQQGKETS 350
    TNSVASIYAW TRGLAHRGKL DGNSDLVKFS ETLERVVVKA IEDGHMTKDL 400
    ALCVYGSSGV KREHYETTEQ FLDSVDTALK KAMSA 435
    Length:435
    Mass (Da):48,490
    Last modified:July 19, 2005 - v1
    Checksum:i685D1D34CB6A5CC3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FR796406 Genomic DNA. Translation: CAJ02471.1.
    RefSeqiXP_001681361.1. XM_001681309.1.

    Genome annotation databases

    EnsemblProtistsiLmjF.10.0290:mRNA; LmjF.10.0290:pep; LmjF.10.0290.
    GeneIDi5649629.
    KEGGilma:LMJF_10_0290.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FR796406 Genomic DNA. Translation: CAJ02471.1 .
    RefSeqi XP_001681361.1. XM_001681309.1.

    3D structure databases

    ProteinModelPortali Q4QHI7.
    SMRi Q4QHI7. Positions 27-433.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi LmjF.10.0290:mRNA ; LmjF.10.0290:pep ; LmjF.10.0290 .
    GeneIDi 5649629.
    KEGGi lma:LMJF_10_0290.

    Phylogenomic databases

    HOGENOMi HOG000019858.
    KOi K00031.
    OMAi KNTIMKV.

    Family and domain databases

    Gene3Di 3.40.718.10. 1 hit.
    InterProi IPR019818. IsoCit/isopropylmalate_DH_CS.
    IPR004790. Isocitrate_DH_NADP.
    IPR024084. IsoPropMal-DH-like_dom.
    [Graphical view ]
    PANTHERi PTHR11822. PTHR11822. 1 hit.
    Pfami PF00180. Iso_dh. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000108. IDH_NADP. 1 hit.
    TIGRFAMsi TIGR00127. nadp_idh_euk. 1 hit.
    PROSITEi PS00470. IDH_IMDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome of the kinetoplastid parasite, Leishmania major."
      Ivens A.C., Peacock C.S., Worthey E.A., Murphy L., Aggarwal G., Berriman M., Sisk E., Rajandream M.A., Adlem E., Aert R., Anupama A., Apostolou Z., Attipoe P., Bason N., Bauser C., Beck A., Beverley S.M., Bianchettin G.
      , Borzym K., Bothe G., Bruschi C.V., Collins M., Cadag E., Ciarloni L., Clayton C., Coulson R.M.R., Cronin A., Cruz A.K., Davies R.M., De Gaudenzi J., Dobson D.E., Duesterhoeft A., Fazelina G., Fosker N., Frasch A.C., Fraser A., Fuchs M., Gabel C., Goble A., Goffeau A., Harris D., Hertz-Fowler C., Hilbert H., Horn D., Huang Y., Klages S., Knights A., Kube M., Larke N., Litvin L., Lord A., Louie T., Marra M., Masuy D., Matthews K., Michaeli S., Mottram J.C., Mueller-Auer S., Munden H., Nelson S., Norbertczak H., Oliver K., O'neil S., Pentony M., Pohl T.M., Price C., Purnelle B., Quail M.A., Rabbinowitsch E., Reinhardt R., Rieger M., Rinta J., Robben J., Robertson L., Ruiz J.C., Rutter S., Saunders D., Schaefer M., Schein J., Schwartz D.C., Seeger K., Seyler A., Sharp S., Shin H., Sivam D., Squares R., Squares S., Tosato V., Vogt C., Volckaert G., Wambutt R., Warren T., Wedler H., Woodward J., Zhou S., Zimmermann W., Smith D.F., Blackwell J.M., Stuart K.D., Barrell B.G., Myler P.J.
      Science 309:436-442(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: MHOM/IL/81/FriedlinImported.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: FriedlinImported.

    Entry informationi

    Entry nameiQ4QHI7_LEIMA
    AccessioniPrimary (citable) accession number: Q4QHI7
    Entry historyi
    Integrated into UniProtKB/TrEMBL: July 19, 2005
    Last sequence update: July 19, 2005
    Last modified: October 1, 2014
    This is version 64 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteomeImported

    External Data

    Dasty 3