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Protein

Isocitrate dehydrogenase [NADP]

Gene

LMJF_10_0290

Organism
Leishmania major
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Isocitrate + NADP+ = 2-oxoglutarate + CO2 + NADPH.UniRule annotation

Cofactori

Mg2+UniRule annotation, Mn2+UniRule annotationNote: Binds 1 Mg(2+) or Mn2+ ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei103 – 1031SubstrateUniRule annotation
Binding sitei108 – 1081NADPUniRule annotation
Binding sitei135 – 1351SubstrateUniRule annotation
Binding sitei158 – 1581SubstrateUniRule annotation
Sitei165 – 1651Critical for catalysisUniRule annotation
Sitei236 – 2361Critical for catalysisUniRule annotation
Metal bindingi276 – 2761Magnesium or manganeseUniRule annotation
Binding sitei284 – 2841NADPUniRule annotation
Metal bindingi299 – 2991Magnesium or manganeseUniRule annotation
Binding sitei352 – 3521NADP; via amide nitrogen and carbonyl oxygenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi101 – 1033NADPUniRule annotation
Nucleotide bindingi334 – 3396NADPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

OxidoreductaseUniRule annotationImported

Keywords - Biological processi

Tricarboxylic acid cycleUniRule annotation

Keywords - Ligandi

MagnesiumUniRule annotation, ManganeseUniRule annotation, Metal-bindingUniRule annotation, NADPUniRule annotation

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate dehydrogenase [NADP]UniRule annotation (EC:1.1.1.42UniRule annotation)
Gene namesi
ORF Names:LMJF_10_0290Imported
OrganismiLeishmania majorImported
Taxonomic identifieri5664 [NCBI]
Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeLeishmaniinaeLeishmania
ProteomesiUP000000542 Componenti: Chromosome 10

Interactioni

Protein-protein interaction databases

STRINGi5664.LmjF.10.0290.

Structurei

3D structure databases

ProteinModelPortaliQ4QHI7.
SMRiQ4QHI7. Positions 27-433.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni120 – 1267Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the isocitrate and isopropylmalate dehydrogenases family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000019858.
InParanoidiQ4QHI7.
KOiK00031.
OMAiQVIEHDV.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11822. PTHR11822. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4QHI7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFRHVSAASA SSLVAARSFS STNVYLDKRI KVKNEVVDMD GDEMTRIIWS
60 70 80 90 100
FIKEKLILPY VDVPINYFDL SVTNRDATND KVTVEAAEAV KKCNVGIKCA
110 120 130 140 150
TITPDEARVK EFNLKKMWKS PNGTIRNILG GTVFREPIIV SNIPRIVPQW
160 170 180 190 200
HNPIVVGRHA FGDQYKATDA VLKPGKLQLV HTPADGSAPT TLDVYDFKGE
210 220 230 240 250
GVGLAMYNTK ESIEGFAKSC FQYALMRKYP LVLTTKNTIL KKYDGMFLQT
260 270 280 290 300
FQRMYDEQYK ADFEKAGITY NHRLIDDQVA QMIKGEGGFV WACKNYDGDV
310 320 330 340 350
QSDIVAQGFG SLGLMTSVLM CPDGKTIEAE AAHGTVTRHY RQHQQGKETS
360 370 380 390 400
TNSVASIYAW TRGLAHRGKL DGNSDLVKFS ETLERVVVKA IEDGHMTKDL
410 420 430
ALCVYGSSGV KREHYETTEQ FLDSVDTALK KAMSA
Length:435
Mass (Da):48,490
Last modified:July 19, 2005 - v1
Checksum:i685D1D34CB6A5CC3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FR796406 Genomic DNA. Translation: CAJ02471.1.
RefSeqiXP_001681361.1. XM_001681309.1.

Genome annotation databases

EnsemblProtistsiLmjF.10.0290:mRNA; LmjF.10.0290:pep; LmjF.10.0290.
GeneIDi5649629.
KEGGilma:LMJF_10_0290.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FR796406 Genomic DNA. Translation: CAJ02471.1.
RefSeqiXP_001681361.1. XM_001681309.1.

3D structure databases

ProteinModelPortaliQ4QHI7.
SMRiQ4QHI7. Positions 27-433.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi5664.LmjF.10.0290.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiLmjF.10.0290:mRNA; LmjF.10.0290:pep; LmjF.10.0290.
GeneIDi5649629.
KEGGilma:LMJF_10_0290.

Phylogenomic databases

HOGENOMiHOG000019858.
InParanoidiQ4QHI7.
KOiK00031.
OMAiQVIEHDV.

Family and domain databases

Gene3Di3.40.718.10. 1 hit.
InterProiIPR019818. IsoCit/isopropylmalate_DH_CS.
IPR004790. Isocitrate_DH_NADP.
IPR024084. IsoPropMal-DH-like_dom.
[Graphical view]
PANTHERiPTHR11822. PTHR11822. 1 hit.
PfamiPF00180. Iso_dh. 1 hit.
[Graphical view]
PIRSFiPIRSF000108. IDH_NADP. 1 hit.
TIGRFAMsiTIGR00127. nadp_idh_euk. 1 hit.
PROSITEiPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome of the kinetoplastid parasite, Leishmania major."
    Ivens A.C., Peacock C.S., Worthey E.A., Murphy L., Aggarwal G., Berriman M., Sisk E., Rajandream M.A., Adlem E., Aert R., Anupama A., Apostolou Z., Attipoe P., Bason N., Bauser C., Beck A., Beverley S.M., Bianchettin G.
    , Borzym K., Bothe G., Bruschi C.V., Collins M., Cadag E., Ciarloni L., Clayton C., Coulson R.M., Cronin A., Cruz A.K., Davies R.M., De Gaudenzi J., Dobson D.E., Duesterhoeft A., Fazelina G., Fosker N., Frasch A.C., Fraser A., Fuchs M., Gabel C., Goble A., Goffeau A., Harris D., Hertz-Fowler C., Hilbert H., Horn D., Huang Y., Klages S., Knights A., Kube M., Larke N., Litvin L., Lord A., Louie T., Marra M., Masuy D., Matthews K., Michaeli S., Mottram J.C., Muller-Auer S., Munden H., Nelson S., Norbertczak H., Oliver K., O'neil S., Pentony M., Pohl T.M., Price C., Purnelle B., Quail M.A., Rabbinowitsch E., Reinhardt R., Rieger M., Rinta J., Robben J., Robertson L., Ruiz J.C., Rutter S., Saunders D., Schafer M., Schein J., Schwartz D.C., Seeger K., Seyler A., Sharp S., Shin H., Sivam D., Squares R., Squares S., Tosato V., Vogt C., Volckaert G., Wambutt R., Warren T., Wedler H., Woodward J., Zhou S., Zimmermann W., Smith D.F., Blackwell J.M., Stuart K.D., Barrell B., Myler P.J.
    Science 309:436-442(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MHOM/IL/81/FriedlinImported.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: FriedlinImported.

Entry informationi

Entry nameiQ4QHI7_LEIMA
AccessioniPrimary (citable) accession number: Q4QHI7
Entry historyi
Integrated into UniProtKB/TrEMBL: July 19, 2005
Last sequence update: July 19, 2005
Last modified: June 24, 2015
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.