ID   GPDA_LEIMA              Reviewed;         367 AA.
AC   Q4QHG4;
DT   28-NOV-2006, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2005, sequence version 1.
DT   16-JUN-2009, entry version 28.
DE   RecName: Full=Glycerol-3-phosphate dehydrogenase [NAD+], glycosomal;
DE            EC=1.1.1.8;
GN   Name=GPD; ORFNames=LmjF10.0510;
OS   Leishmania major.
OC   Eukaryota; Euglenozoa; Kinetoplastida; Trypanosomatidae; Leishmania.
OX   NCBI_TaxID=5664;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MHOM/IL/81/Friedlin;
RX   PubMed=16020728; DOI=10.1126/science.1112680;
RA   Ivens A.C., Peacock C.S., Worthey E.A., Murphy L., Aggarwal G.,
RA   Berriman M., Sisk E., Rajandream M.A., Adlem E., Aert R., Anupama A.,
RA   Apostolou Z., Attipoe P., Bason N., Bauser C., Beck A., Beverley S.M.,
RA   Bianchettin G., Borzym K., Bothe G., Bruschi C.V., Collins M.,
RA   Cadag E., Ciarloni L., Clayton C., Coulson R.M.R., Cronin A.,
RA   Cruz A.K., Davies R.M., De Gaudenzi J., Dobson D.E., Duesterhoeft A.,
RA   Fazelina G., Fosker N., Frasch A.C., Fraser A., Fuchs M., Gabel C.,
RA   Goble A., Goffeau A., Harris D., Hertz-Fowler C., Hilbert H., Horn D.,
RA   Huang Y., Klages S., Knights A., Kube M., Larke N., Litvin L.,
RA   Lord A., Louie T., Marra M., Masuy D., Matthews K., Michaeli S.,
RA   Mottram J.C., Mueller-Auer S., Munden H., Nelson S., Norbertczak H.,
RA   Oliver K., O'neil S., Pentony M., Pohl T.M., Price C., Purnelle B.,
RA   Quail M.A., Rabbinowitsch E., Reinhardt R., Rieger M., Rinta J.,
RA   Robben J., Robertson L., Ruiz J.C., Rutter S., Saunders D.,
RA   Schaefer M., Schein J., Schwartz D.C., Seeger K., Seyler A., Sharp S.,
RA   Shin H., Sivam D., Squares R., Squares S., Tosato V., Vogt C.,
RA   Volckaert G., Wambutt R., Warren T., Wedler H., Woodward J., Zhou S.,
RA   Zimmermann W., Smith D.F., Blackwell J.M., Stuart K.D., Barrell B.G.,
RA   Myler P.J.;
RT   "The genome of the kinetoplastid parasite, Leishmania major.";
RL   Science 309:436-442(2005).
CC   -!- CATALYTIC ACTIVITY: sn-glycerol 3-phosphate + NAD(+) = glycerone
CC       phosphate + NADH.
CC   -!- SUBUNIT: Homodimer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Glycosome (By similarity).
CC   -!- SIMILARITY: Belongs to the NAD-dependent glycerol-3-phosphate
CC       dehydrogenase family.
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DR   EMBL; CT005249; CAJ02624.1; -; Genomic_DNA.
DR   RefSeq; XP_001681384.1; -.
DR   SMR; Q4QHG4; 11-358.
DR   GeneID; 5649653; -.
DR   KEGG; lma:LmjF10.0510; -.
DR   HOGENOM; Q4QHG4; -.
DR   OMA; Q4QHG4; WHIKEEE.
DR   BRENDA; 1.1.1.8; 19061.
DR   GO; GO:0009331; C:glycerol-3-phosphate dehydrogenase complex; IEA:InterPro.
DR   GO; GO:0020015; C:glycosome; IEA:UniProtKB-SubCell.
DR   GO; GO:0004367; F:glycerol-3-phosphate dehydrogenase (NAD+) a...; IEA:EC.
DR   GO; GO:0051287; F:NAD or NADH binding; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:0046168; P:glycerol-3-phosphate catabolic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR013328; DH_multihelical.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   InterPro; IPR006168; NAD-dep_Gly3P_DH.
DR   InterPro; IPR011128; NAD-dep_Gly3P_DH_N.
DR   InterPro; IPR006109; NAD_Gly3P_DH_C.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Gene3D; G3DSA:1.10.1040.10; Opine_DH; 1.
DR   PANTHER; PTHR11728; NAD_Gly3P_DH; 1.
DR   Pfam; PF07479; NAD_Gly3P_dh_C; 1.
DR   Pfam; PF01210; NAD_Gly3P_dh_N; 1.
DR   PIRSF; PIRSF000114; Glycerol-3-P_dh; 1.
DR   PRINTS; PR00077; GPDHDRGNASE.
DR   ProDom; PD001278; NAD_Gly3P_C; 1.
DR   PROSITE; PS00957; NAD_G3PDH; 1.
PE   3: Inferred from homology;
KW   Glycosome; NAD; Oxidoreductase.
FT   CHAIN         1    367       Glycerol-3-phosphate dehydrogenase
FT                                [NAD+], glycosomal.
FT                                /FTId=PRO_0000262291.
FT   NP_BIND      23     28       NAD (By similarity).
FT   REGION      275    276       Substrate binding (By similarity).
FT   MOTIF       365    367       Microbody targeting signal (Potential).
FT   ACT_SITE    211    211       Proton acceptor (Potential).
FT   BINDING      98     98       NAD (By similarity).
FT   BINDING     126    126       Substrate (By similarity).
FT   BINDING     158    158       NAD; via amide nitrogen (By similarity).
FT   BINDING     275    275       NAD (By similarity).
FT   BINDING     297    297       NAD; via amide nitrogen (By similarity).
FT   BINDING     301    301       NAD (By similarity).
SQ   SEQUENCE   367 AA;  39299 MW;  49F47113F30B5657 CRC64;
     MISTKRHINT NELLHLSKAV VFGSGAFGTA LAMVLSKKCR EVCVWHIKEE EARLVNEKRE
     NDLYLRGVQL ASNIIFTSDV DEAYKGAELI LFVIPTQFLR GFFQKSGGNL IAYAKARQVP
     VLVCTKGIER STLKFPAQIV GEFFPSNLLS VLAGPSFAIE VATGVFTCVS VASADINVAR
     RLQRIMTTGD RSFVCWATTD TVGCEVASAV KNVLAIGSGV ANGLGMGLNA RAALITRGLL
     EIRDLTAALG GDGSAIFGLA GFGDLQLTCS SELSRNFTVG KKLGKGLSLE EIQRTSKAVA
     EGVATAEPLV RLAQQLKVTM PLCQQIYEVV YKKKNPRAAI ADLLSCGLQD EGLPPLFKKS
     AATPSKL
//