Reviewed, UniProtKB/Swiss-Prot Q4QHG4 (GPDA_LEIMA)

Names and origin

Protein names

Recommended name:
    Glycerol-3-phosphate dehydrogenase [NAD+], glycosomal
    EC=1.1.1.8

Gene names

Name:	GPD
ORF Names:	LmjF10.0510

Organism

Leishmania major

Taxonomic identifier

5664 [NCBI]

Taxonomic lineage

Eukaryota › Euglenozoa › Kinetoplastida › Trypanosomatidae › Leishmania

Protein attributes

Sequence length	367 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH.
Subunit structure	Homodimer By similarity.
Subcellular location	Glycosome By similarity.
Sequence similarities	Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.

Ontologies

Keywords
Cellular component	Glycosome Peroxisome
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	glycerol-3-phosphate catabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	glycerol-3-phosphate dehydrogenase complex Inferred from electronic annotation. Source: InterPro glycosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro glycerol-3-phosphate dehydrogenase (NAD+) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 367	367	Glycerol-3-phosphate dehydrogenase [NAD+], glycosomal		PRO_0000262291
Regions
	Nucleotide binding	23 – 28	6	NAD By similarity
	Region	275 – 276	2	Substrate binding By similarity
	Motif	365 – 367	3	Microbody targeting signal Potential
Sites
	Active site	211	1	Proton acceptor Potential
	Binding site	98	1	NAD By similarity
	Binding site	126	1	Substrate By similarity
	Binding site	158	1	NAD; via amide nitrogen By similarity
	Binding site	275	1	NAD By similarity
	Binding site	297	1	NAD; via amide nitrogen By similarity
	Binding site	301	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4QHG4-1 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: 49F47113F30B5657	FASTA	367	39,299
	10 20 30 40 50 60 MISTKRHINT NELLHLSKAV VFGSGAFGTA LAMVLSKKCR EVCVWHIKEE EARLVNEKRE 70 80 90 100 110 120 NDLYLRGVQL ASNIIFTSDV DEAYKGAELI LFVIPTQFLR GFFQKSGGNL IAYAKARQVP 130 140 150 160 170 180 VLVCTKGIER STLKFPAQIV GEFFPSNLLS VLAGPSFAIE VATGVFTCVS VASADINVAR 190 200 210 220 230 240 RLQRIMTTGD RSFVCWATTD TVGCEVASAV KNVLAIGSGV ANGLGMGLNA RAALITRGLL 250 260 270 280 290 300 EIRDLTAALG GDGSAIFGLA GFGDLQLTCS SELSRNFTVG KKLGKGLSLE EIQRTSKAVA 310 320 330 340 350 360 EGVATAEPLV RLAQQLKVTM PLCQQIYEVV YKKKNPRAAI ADLLSCGLQD EGLPPLFKKS AATPSKL

References

[1]

"The genome of the kinetoplastid parasite, Leishmania major." Ivens A.C., Peacock C.S., Worthey E.A., Murphy L., Aggarwal G., Berriman M., Sisk E., Rajandream M.A., Adlem E., Aert R., Anupama A., Apostolou Z., Attipoe P., Bason N., Bauser C., Beck A., Beverley S.M., Bianchettin G. , Borzym K., Bothe G., Bruschi C.V., Collins M., Cadag E., Ciarloni L., Clayton C., Coulson R.M.R., Cronin A., Cruz A.K., Davies R.M., De Gaudenzi J., Dobson D.E., Duesterhoeft A., Fazelina G., Fosker N., Frasch A.C., Fraser A., Fuchs M., Gabel C., Goble A., Goffeau A., Harris D., Hertz-Fowler C., Hilbert H., Horn D., Huang Y., Klages S., Knights A., Kube M., Larke N., Litvin L., Lord A., Louie T., Marra M., Masuy D., Matthews K., Michaeli S., Mottram J.C., Mueller-Auer S., Munden H., Nelson S., Norbertczak H., Oliver K., O'neil S., Pentony M., Pohl T.M., Price C., Purnelle B., Quail M.A., Rabbinowitsch E., Reinhardt R., Rieger M., Rinta J., Robben J., Robertson L., Ruiz J.C., Rutter S., Saunders D., Schaefer M., Schein J., Schwartz D.C., Seeger K., Seyler A., Sharp S., Shin H., Sivam D., Squares R., Squares S., Tosato V., Vogt C., Volckaert G., Wambutt R., Warren T., Wedler H., Woodward J., Zhou S., Zimmermann W., Smith D.F., Blackwell J.M., Stuart K.D., Barrell B.G., Myler P.J. Science 309:436-442(2005) [PubMed: 16020728] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MHOM/IL/81/Friedlin.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CT005249 Genomic DNA. Translation: CAJ02624.1.
RefSeq	XP_001681384.1.
3D structure databases
SMR	Q4QHG4. Positions 11-358.
ModBase	Search...
Genome annotation databases
GeneID	5649653.
GenomeReviews	Gene locus GPD in contig CT005249_GR.
KEGG	lma:LmjF10.0510.
Phylogenomic databases
HOGENOM	HBG586392.
OMA	YTPIASE.
PhylomeDB	Q4QHG4.
Enzyme and pathway databases
BRENDA	1.1.1.8. 19061.
Family and domain databases
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR013328. DH_multihelical. IPR016040. NAD(P)-bd_dom. IPR006168. NAD-dep_Gly3P_DH. IPR011128. NAD-dep_Gly3P_DH_N. IPR006109. NAD_Gly3P_DH_C. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
PANTHER	PTHR11728. NAD_Gly3P_DH. 1 hit.
Pfam	PF07479. NAD_Gly3P_dh_C. 1 hit. PF01210. NAD_Gly3P_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000114. Glycerol-3-P_dh. 1 hit.
PRINTS	PR00077. GPDHDRGNASE.
PROSITE	PS00957. NAD_G3PDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GPDA_LEIMA

Accession

Primary (citable) accession number: Q4QHG4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	July 19, 2005
Last modified:	February 9, 2010
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)