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Q4QEW7

- Q4QEW7_LEIMA

UniProt

Q4QEW7 - Q4QEW7_LEIMA

Protein
Submitted name:

Dihydroorotate dehydrogenase

Gene

DHODH

Organism
Leishmania major
Status
Unreviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 1 (19 Jul 2005)
      Previous versions | rss
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    Functioni

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei20 – 201FMNImported
    Binding sitei68 – 681FMNImported
    Binding sitei128 – 1281FMNImported
    Binding sitei165 – 1651FMNImported
    Binding sitei223 – 2231FMN; via amide nitrogenImported

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi44 – 452FMNImported
    Nucleotide bindingi194 – 1952FMNImported
    Nucleotide bindingi249 – 2513FMNImported
    Nucleotide bindingi272 – 2732FMNImported

    GO - Molecular functioni

    1. dihydroorotate dehydrogenase activity Source: GeneDB_Lmajor
    2. dihydroorotate oxidase activity Source: UniProtKB-EC
    3. nucleotide binding Source: UniProtKB-KW

    GO - Biological processi

    1. 'de novo' pyrimidine nucleobase biosynthetic process Source: GeneDB_Lmajor
    2. fumarate metabolic process Source: GeneDB_Lmajor
    3. UMP biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    OxidoreductaseImported

    Keywords - Ligandi

    Flavoprotein, FMNImported, Nucleotide-bindingImported

    Names & Taxonomyi

    Protein namesi
    Submitted name:
    Dihydroorotate dehydrogenaseImported (EC:1.3.3.1Imported)
    Gene namesi
    Name:DHODHImported
    ORF Names:LMJF_16_0530Imported
    OrganismiLeishmania majorImported
    Taxonomic identifieri5664 [NCBI]
    Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeLeishmaniinaeLeishmania
    ProteomesiUP000000542: Chromosome 16

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3GYEX-ray2.00A/B1-320[»]
    3GZ3X-ray1.90A/B1-320[»]
    3MHUX-ray1.85A/B1-312[»]
    3MJYX-ray1.96A/B1-312[»]
    3TJXX-ray1.64A/B1-320[»]
    3TQ0X-ray1.90A/B1-312[»]
    3TROX-ray1.86A/B1-320[»]
    4EF8X-ray1.56A/B1-320[»]
    4EF9X-ray1.60A/B1-320[»]
    ProteinModelPortaliQ4QEW7.
    SMRiQ4QEW7. Positions 1-313.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ4QEW7.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni160 – 1612Sulfate binding

    Phylogenomic databases

    HOGENOMiHOG000225104.
    KOiK00226.
    OMAiYELYEAC.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    InterProiIPR013785. Aldolase_TIM.
    IPR012135. Dihydroorotate_DH_1_2.
    [Graphical view]
    PfamiPF01180. DHO_dh. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000164. DHO_oxidase. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q4QEW7-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSLQVNLLNN TFANPFMNAA GVMCTTTEEL VAMTESASGS LVSKSCTPAL    50
    REGNPTPRYQ ALPLGSINSM GLPNNGFDFY LAYAAEQHDY GKKPLFLSMS 100
    GLSMRENVEM CKRLAAVATE KGVILELNLS CPNVPGKPQV AYDFDAMRQC 150
    LTAVSEVYPH SFGVKMPPYF DFAHFDAAAE ILNEFPKVQF ITCINSIGNG 200
    LVIDAETESV VIKPKQGFGG LGGRYVLPTA LANINAFYRR CPGKLIFGCG 250
    GVYTGEDAFL HVLAGASMVQ VGTALQEEGP SIFERLTSEL LGVMAKKRYQ 300
    TLDEFRGKVR TLDGTAESTR 320
    Length:320
    Mass (Da):34,662
    Last modified:July 19, 2005 - v1
    Checksum:iFB13D0101F91577A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FR796412 Genomic DNA. Translation: CAJ03555.1.
    RefSeqiXP_001682131.1. XM_001682079.1.

    Genome annotation databases

    EnsemblProtistsiLmjF.16.0530:mRNA; LmjF.16.0530:pep; LmjF.16.0530.
    GeneIDi5650598.
    KEGGilma:LMJF_16_0530.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FR796412 Genomic DNA. Translation: CAJ03555.1 .
    RefSeqi XP_001682131.1. XM_001682079.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3GYE X-ray 2.00 A/B 1-320 [» ]
    3GZ3 X-ray 1.90 A/B 1-320 [» ]
    3MHU X-ray 1.85 A/B 1-312 [» ]
    3MJY X-ray 1.96 A/B 1-312 [» ]
    3TJX X-ray 1.64 A/B 1-320 [» ]
    3TQ0 X-ray 1.90 A/B 1-312 [» ]
    3TRO X-ray 1.86 A/B 1-320 [» ]
    4EF8 X-ray 1.56 A/B 1-320 [» ]
    4EF9 X-ray 1.60 A/B 1-320 [» ]
    ProteinModelPortali Q4QEW7.
    SMRi Q4QEW7. Positions 1-313.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi LmjF.16.0530:mRNA ; LmjF.16.0530:pep ; LmjF.16.0530 .
    GeneIDi 5650598.
    KEGGi lma:LMJF_16_0530.

    Phylogenomic databases

    HOGENOMi HOG000225104.
    KOi K00226.
    OMAi YELYEAC.

    Miscellaneous databases

    EvolutionaryTracei Q4QEW7.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    InterProi IPR013785. Aldolase_TIM.
    IPR012135. Dihydroorotate_DH_1_2.
    [Graphical view ]
    Pfami PF01180. DHO_dh. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000164. DHO_oxidase. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The genome of the kinetoplastid parasite, Leishmania major."
      Ivens A.C., Peacock C.S., Worthey E.A., Murphy L., Aggarwal G., Berriman M., Sisk E., Rajandream M.A., Adlem E., Aert R., Anupama A., Apostolou Z., Attipoe P., Bason N., Bauser C., Beck A., Beverley S.M., Bianchettin G.
      , Borzym K., Bothe G., Bruschi C.V., Collins M., Cadag E., Ciarloni L., Clayton C., Coulson R.M.R., Cronin A., Cruz A.K., Davies R.M., De Gaudenzi J., Dobson D.E., Duesterhoeft A., Fazelina G., Fosker N., Frasch A.C., Fraser A., Fuchs M., Gabel C., Goble A., Goffeau A., Harris D., Hertz-Fowler C., Hilbert H., Horn D., Huang Y., Klages S., Knights A., Kube M., Larke N., Litvin L., Lord A., Louie T., Marra M., Masuy D., Matthews K., Michaeli S., Mottram J.C., Mueller-Auer S., Munden H., Nelson S., Norbertczak H., Oliver K., O'neil S., Pentony M., Pohl T.M., Price C., Purnelle B., Quail M.A., Rabbinowitsch E., Reinhardt R., Rieger M., Rinta J., Robben J., Robertson L., Ruiz J.C., Rutter S., Saunders D., Schaefer M., Schein J., Schwartz D.C., Seeger K., Seyler A., Sharp S., Shin H., Sivam D., Squares R., Squares S., Tosato V., Vogt C., Volckaert G., Wambutt R., Warren T., Wedler H., Woodward J., Zhou S., Zimmermann W., Smith D.F., Blackwell J.M., Stuart K.D., Barrell B.G., Myler P.J.
      Science 309:436-442(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: MHOM/IL/81/FriedlinImported.
    2. "Crystal Structure of Leishmania major Dihydroorotate dehydrogenase."
      Cordeiro A.T., Feliciano P.R., Nonato M.C.
      Submitted (APR-2009) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) IN COMPLEX WITH FMN.
    3. "Leismania major dihydroorotate in complex with orotate."
      Cordeiro A.T., Feliciano P.R., Nonato M.C.
      Submitted (APR-2009) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) IN COMPLEX WITH FMN.
    4. "Novel insights for dihydroorotate dehydrogenase class 1A inhibitors discovery."
      Cheleski J., Rocha J.R., Pinheiro M.P., Wiggers H.J., da Silva A.B., Nonato M.C., Montanari C.A.
      Eur. J. Med. Chem. 45:5899-5909(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) OF 1-312 IN COMPLEX WITH FMN.
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: FriedlinImported.
    6. "Crystal Structure of Leishmania major dihydroorotate dehydrogenase mutant H174A."
      de Souza A.L., Nonato M.C., Pinheiro M.P.
      Submitted (AUG-2011) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (1.64 ANGSTROMS) IN COMPLEX WITH FMN.
    7. "Crystal Structure of Leishmania major dihydroorotate dehydrogenase mutant D171A."
      de Souza A.L., Pinheiro M.P., Nonato M.C.
      Submitted (SEP-2011) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (1.86 ANGSTROMS) IN COMPLEX WITH FMN.
    8. "Crystal structure of dihydroorotate dehydrogenase from Leishmania major."
      Cordeiro A.T., Feliciano P.R., Pinheiro M.P., Nonato M.C.
      Biochimie 94:1739-1748(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) OF 1-312 IN COMPLEX WITH FMN.
    9. "Target sites for the design of anti-trypanosomatid drugs based on the structure of dihydroorotate dehydrogenase."
      Pinheiro M.P., Emery F.d.a. .S., Nonato M.C.
      Curr. Pharm. Des. 19:2615-2627(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.56 ANGSTROMS) IN COMPLEX WITH FMN.

    Entry informationi

    Entry nameiQ4QEW7_LEIMA
    AccessioniPrimary (citable) accession number: Q4QEW7
    Entry historyi
    Integrated into UniProtKB/TrEMBL: July 19, 2005
    Last sequence update: July 19, 2005
    Last modified: October 1, 2014
    This is version 78 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported, Complete proteome, Reference proteomeImported

    External Data

    Dasty 3