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Q4QEW7 (Q4QEW7_LEIMA) Unreviewed, UniProtKB/TrEMBL

Last modified June 11, 2014. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein names
EC=1.3.3.1 EMBL CAJ03555.1
Gene names
Name:DHODH EMBL CAJ03555.1
ORF Names:LMJF_16_0530 EMBL CAJ03555.1
OrganismLeishmania major [Reference proteome]
Taxonomic identifier5664 [NCBI]
Taxonomic lineageEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeLeishmaniinaeLeishmania

Protein attributes

Sequence length320 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Nucleotide binding44 – 452FMN PDB 3TQ0 PDB 3TJX PDB 3GYE PDB 3GZ3 PDB 3TRO PDB 3MHU PDB 3MJY PDB 4EF8 PDB 4EF9
Nucleotide binding194 – 1952FMN PDB 3TQ0 PDB 3TJX PDB 3GYE PDB 3GZ3 PDB 3TRO PDB 3MHU PDB 3MJY PDB 4EF8 PDB 4EF9
Nucleotide binding249 – 2513FMN PDB 3TQ0 PDB 3TJX PDB 3GYE PDB 3GZ3 PDB 3TRO PDB 3MHU PDB 3MJY PDB 4EF8 PDB 4EF9
Nucleotide binding272 – 2732FMN PDB 3TQ0 PDB 3TJX PDB 3GYE PDB 3GZ3 PDB 3TRO PDB 3MHU PDB 3MJY PDB 4EF8 PDB 4EF9
Region69 – 713Sulfate 3 binding PDB 3TJX
Region71 – 722Sulfate 2 binding PDB 4EF9
Region74 – 752Sulfate 1 binding PDB 4EF9
Region104 – 1052Sulfate 5 binding PDB 4EF8
Region160 – 1612Sulfate 6 binding PDB 3TQ0
Region160 – 1612Sulfate 8 binding PDB 3TJX PDB 3TRO PDB 3MHU PDB 3MJY PDB 4EF8 PDB 4EF9
Region160 – 1612Sulfate binding

Sites

Binding site201FMN PDB 3TQ0 PDB 3TJX PDB 3GYE PDB 3GZ3 PDB 3TRO PDB 3MHU PDB 3MJY PDB 4EF8 PDB 4EF9
Binding site441Sulfate 2 PDB 4EF9
Binding site591Sulfate 4 PDB 4EF9
Binding site681FMN PDB 3TQ0 PDB 3TJX PDB 3GYE PDB 3GZ3 PDB 3TRO PDB 3MHU PDB 3MJY PDB 4EF8 PDB 4EF9
Binding site1281FMN PDB 3TQ0 PDB 3TJX PDB 3GYE PDB 3GZ3 PDB 3TRO PDB 3MHU PDB 3MJY PDB 4EF8 PDB 4EF9
Binding site1311Sulfate 2 PDB 4EF9
Binding site1651FMN PDB 3TQ0 PDB 3TJX PDB 3GYE PDB 3GZ3 PDB 3TRO PDB 3MHU PDB 3MJY PDB 4EF8 PDB 4EF9
Binding site1871Sulfate 7 PDB 3TQ0 PDB 3TJX PDB 3TRO
Binding site2161Sulfate 10 PDB 3TJX
Binding site2231FMN; via amide nitrogen PDB 3TQ0 PDB 3TJX PDB 3GYE PDB 3GZ3 PDB 3TRO PDB 3MHU PDB 3MJY PDB 4EF8 PDB 4EF9
Binding site2381Sulfate 11 PDB 3TJX
Binding site2381Sulfate 12 PDB 3TQ0
Binding site2391Sulfate 13 PDB 3TQ0 PDB 3TJX
Binding site3111Sulfate 13 PDB 3TJX

Sequences

Sequence LengthMass (Da)Tools
Q4QEW7 [UniParc].

Last modified July 19, 2005. Version 1.
Checksum: FB13D0101F91577A

FASTA32034,662
        10         20         30         40         50         60 
MSLQVNLLNN TFANPFMNAA GVMCTTTEEL VAMTESASGS LVSKSCTPAL REGNPTPRYQ 

        70         80         90        100        110        120 
ALPLGSINSM GLPNNGFDFY LAYAAEQHDY GKKPLFLSMS GLSMRENVEM CKRLAAVATE 

       130        140        150        160        170        180 
KGVILELNLS CPNVPGKPQV AYDFDAMRQC LTAVSEVYPH SFGVKMPPYF DFAHFDAAAE 

       190        200        210        220        230        240 
ILNEFPKVQF ITCINSIGNG LVIDAETESV VIKPKQGFGG LGGRYVLPTA LANINAFYRR 

       250        260        270        280        290        300 
CPGKLIFGCG GVYTGEDAFL HVLAGASMVQ VGTALQEEGP SIFERLTSEL LGVMAKKRYQ 

       310        320 
TLDEFRGKVR TLDGTAESTR 

« Hide

References

« Hide 'large scale' references
[1]"The genome of the kinetoplastid parasite, Leishmania major."
Ivens A.C., Peacock C.S., Worthey E.A., Murphy L., Aggarwal G., Berriman M., Sisk E., Rajandream M.A., Adlem E., Aert R., Anupama A., Apostolou Z., Attipoe P., Bason N., Bauser C., Beck A., Beverley S.M., Bianchettin G. expand/collapse author list , Borzym K., Bothe G., Bruschi C.V., Collins M., Cadag E., Ciarloni L., Clayton C., Coulson R.M.R., Cronin A., Cruz A.K., Davies R.M., De Gaudenzi J., Dobson D.E., Duesterhoeft A., Fazelina G., Fosker N., Frasch A.C., Fraser A., Fuchs M., Gabel C., Goble A., Goffeau A., Harris D., Hertz-Fowler C., Hilbert H., Horn D., Huang Y., Klages S., Knights A., Kube M., Larke N., Litvin L., Lord A., Louie T., Marra M., Masuy D., Matthews K., Michaeli S., Mottram J.C., Mueller-Auer S., Munden H., Nelson S., Norbertczak H., Oliver K., O'neil S., Pentony M., Pohl T.M., Price C., Purnelle B., Quail M.A., Rabbinowitsch E., Reinhardt R., Rieger M., Rinta J., Robben J., Robertson L., Ruiz J.C., Rutter S., Saunders D., Schaefer M., Schein J., Schwartz D.C., Seeger K., Seyler A., Sharp S., Shin H., Sivam D., Squares R., Squares S., Tosato V., Vogt C., Volckaert G., Wambutt R., Warren T., Wedler H., Woodward J., Zhou S., Zimmermann W., Smith D.F., Blackwell J.M., Stuart K.D., Barrell B.G., Myler P.J.
Science 309:436-442(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MHOM/IL/81/Friedlin.
[2]"Crystal Structure of Leishmania major Dihydroorotate dehydrogenase."
Cordeiro A.T., Feliciano P.R., Nonato M.C.
Submitted (APR-2009) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (2.00 ANGSTROMS) IN COMPLEX WITH FMN.
[3]"Leismania major dihydroorotate in complex with orotate."
Cordeiro A.T., Feliciano P.R., Nonato M.C.
Submitted (APR-2009) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) IN COMPLEX WITH FMN.
[4]"Novel insights for dihydroorotate dehydrogenase class 1A inhibitors discovery."
Cheleski J., Rocha J.R., Pinheiro M.P., Wiggers H.J., da Silva A.B., Nonato M.C., Montanari C.A.
Eur. J. Med. Chem. 45:5899-5909(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS) OF 1-312 IN COMPLEX WITH FMN AND SULFATE.
[5]"Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania."
Rogers M.B., Hilley J.D., Dickens N.J., Wilkes J., Bates P.A., Depledge D.P., Harris D., Her Y., Herzyk P., Imamura H., Otto T.D., Sanders M., Seeger K., Dujardin J.C., Berriman M., Smith D.F., Hertz-Fowler C., Mottram J.C.
Genome Res. 21:2129-2142(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Friedlin EMBL CAJ03555.1.
[6]"Crystal Structure of Leishmania major dihydroorotate dehydrogenase mutant H174A."
de Souza A.L., Nonato M.C., Pinheiro M.P.
Submitted (AUG-2011) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (1.64 ANGSTROMS) IN COMPLEX WITH FMN AND SULFATE.
[7]"Crystal Structure of Leishmania major dihydroorotate dehydrogenase mutant D171A."
de Souza A.L., Pinheiro M.P., Nonato M.C.
Submitted (SEP-2011) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (1.86 ANGSTROMS) IN COMPLEX WITH FMN AND SULFATE.
[8]"Crystal structure of dihydroorotate dehydrogenase from Leishmania major."
Cordeiro A.T., Feliciano P.R., Pinheiro M.P., Nonato M.C.
Biochimie 94:1739-1748(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) OF 1-312 IN COMPLEX WITH FMN AND SULFATE.
[9]"Target sites for the design of anti-trypanosomatid drugs based on the structure of dihydroorotate dehydrogenase."
Pinheiro M.P., Emery F.d.a. .S., Nonato M.C.
Curr. Pharm. Des. 19:2615-2627(2013) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.56 ANGSTROMS) IN COMPLEX WITH FMN AND SULFATE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
FR796412 Genomic DNA. Translation: CAJ03555.1.
RefSeqXP_001682131.1. XM_001682079.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3GYEX-ray2.00A/B1-320[»]
3GZ3X-ray1.90A/B1-320[»]
3MHUX-ray1.85A/B1-312[»]
3MJYX-ray1.96A/B1-312[»]
3TJXX-ray1.64A/B1-320[»]
3TQ0X-ray1.90A/B1-312[»]
3TROX-ray1.86A/B1-320[»]
4EF8X-ray1.56A/B1-320[»]
4EF9X-ray1.60A/B1-320[»]
ProteinModelPortalQ4QEW7.
SMRQ4QEW7. Positions 1-313.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsLmjF.16.0530:mRNA; LmjF.16.0530:pep; LmjF.16.0530.
GeneID5650598.
KEGGlma:LMJF_16_0530.

Phylogenomic databases

HOGENOMHOG000225104.
KOK00226.
OMAYELYEAC.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
InterProIPR013785. Aldolase_TIM.
IPR012135. Dihydroorotate_DH_1_2.
[Graphical view]
PfamPF01180. DHO_dh. 1 hit.
[Graphical view]
PIRSFPIRSF000164. DHO_oxidase. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceQ4QEW7.

Entry information

Entry nameQ4QEW7_LEIMA
AccessionPrimary (citable) accession number: Q4QEW7
Entry history
Integrated into UniProtKB/TrEMBL: July 19, 2005
Last sequence update: July 19, 2005
Last modified: June 11, 2014
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)