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Protein

Probable citrate synthase, mitochondrial

Gene

LmjF18.0680

Organism
Leishmania major
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Acetyl-CoA + H2O + oxaloacetate = citrate + CoA.PROSITE-ProRule annotation

Pathway:itricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes isocitrate from oxaloacetate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Probable citrate synthase, mitochondrial (LmjF18.0680)
  2. no protein annotated in this organism
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isocitrate from oxaloacetate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei297 – 2971PROSITE-ProRule annotation
Active sitei351 – 3511PROSITE-ProRule annotation
Active sitei406 – 4061PROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Tricarboxylic acid cycle

Enzyme and pathway databases

UniPathwayiUPA00223; UER00717.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable citrate synthase, mitochondrial (EC:2.3.3.16)
Gene namesi
ORF Names:LmjF18.0680, LmjF_18_0680
OrganismiLeishmania major
Taxonomic identifieri5664 [NCBI]
Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeLeishmaniinaeLeishmania
ProteomesiUP000000542 Componenti: Chromosome 18

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 470Probable citrate synthase, mitochondrialPRO_0000291606
Transit peptidei1 – ?MitochondrionSequence Analysis

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi5664.LmjF.18.0680.

Structurei

3D structure databases

ProteinModelPortaliQ4QDX3.
SMRiQ4QDX3. Positions 24-463.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the citrate synthase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOGENOMiHOG000130831.
InParanoidiQ4QDX3.
KOiK01647.
OMAiINRTKYW.

Family and domain databases

Gene3Di1.10.580.10. 1 hit.
InterProiIPR016142. Citrate_synth-like_lrg_a-sub.
IPR002020. Citrate_synthase.
IPR019810. Citrate_synthase_AS.
IPR010109. Citrate_synthase_euk.
[Graphical view]
PANTHERiPTHR11739. PTHR11739. 1 hit.
PfamiPF00285. Citrate_synt. 1 hit.
[Graphical view]
PRINTSiPR00143. CITRTSNTHASE.
SUPFAMiSSF48256. SSF48256. 1 hit.
TIGRFAMsiTIGR01793. cit_synth_euk. 1 hit.
PROSITEiPS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q4QDX3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRAVRCSLIR GVAGLRMASS ALDEMKEQML RRWKEDQKKI DDLRKKHGHE
60 70 80 90 100
KLCDATIDAV YGGMRGITGL VYEPSLLDPA EGIRFRGLTI LECQEMLPKA
110 120 130 140 150
PGGKEPLPEA MFWLLMTGEV PTEEQVRGLN AELHRRADPE AIAAAQKAIA
160 170 180 190 200
ALPRNAHPMT AFSVGVLALQ SYSKFAAAYA AGKSNKKTYW EYALEDSLDM
210 220 230 240 250
LARTPTVAAM IYNRETKGQV ELAAPSNSDL DWAANFAKMM GYQDEEFWEC
260 270 280 290 300
MRLYLSVHAD HEGGNVSAHT TTLVASALSD PYLAFSAGLN GLAGPLHGLA
310 320 330 340 350
NQEVLNYLLS MQERVKADGV NMHDEAALEK ALSNYTWELL NSGQVVPGYG
360 370 380 390 400
HAVLRKVDPR YTCQRNFCLR HKFDDDLFKL VNTIYSIMPG ILKEHGKTKN
410 420 430 440 450
PYPNVDAHSG VLLQHYGLTE QNYYTVLFGL SRQMGVLAGV VWDRLQGRPL
460 470
ERPKSITTEM LAKKYLCNSL
Length:470
Mass (Da):52,329
Last modified:July 19, 2005 - v1
Checksum:i5257C02EC31A2E0A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FR796414 Genomic DNA. Translation: CAJ03695.1.
RefSeqiXP_001682475.1. XM_001682423.1.

Genome annotation databases

GeneIDi5650994.
KEGGilma:LMJF_18_0680.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FR796414 Genomic DNA. Translation: CAJ03695.1.
RefSeqiXP_001682475.1. XM_001682423.1.

3D structure databases

ProteinModelPortaliQ4QDX3.
SMRiQ4QDX3. Positions 24-463.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi5664.LmjF.18.0680.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi5650994.
KEGGilma:LMJF_18_0680.

Phylogenomic databases

HOGENOMiHOG000130831.
InParanoidiQ4QDX3.
KOiK01647.
OMAiINRTKYW.

Enzyme and pathway databases

UniPathwayiUPA00223; UER00717.

Family and domain databases

Gene3Di1.10.580.10. 1 hit.
InterProiIPR016142. Citrate_synth-like_lrg_a-sub.
IPR002020. Citrate_synthase.
IPR019810. Citrate_synthase_AS.
IPR010109. Citrate_synthase_euk.
[Graphical view]
PANTHERiPTHR11739. PTHR11739. 1 hit.
PfamiPF00285. Citrate_synt. 1 hit.
[Graphical view]
PRINTSiPR00143. CITRTSNTHASE.
SUPFAMiSSF48256. SSF48256. 1 hit.
TIGRFAMsiTIGR01793. cit_synth_euk. 1 hit.
PROSITEiPS00480. CITRATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome of the kinetoplastid parasite, Leishmania major."
    Ivens A.C., Peacock C.S., Worthey E.A., Murphy L., Aggarwal G., Berriman M., Sisk E., Rajandream M.A., Adlem E., Aert R., Anupama A., Apostolou Z., Attipoe P., Bason N., Bauser C., Beck A., Beverley S.M., Bianchettin G.
    , Borzym K., Bothe G., Bruschi C.V., Collins M., Cadag E., Ciarloni L., Clayton C., Coulson R.M.R., Cronin A., Cruz A.K., Davies R.M., De Gaudenzi J., Dobson D.E., Duesterhoeft A., Fazelina G., Fosker N., Frasch A.C., Fraser A., Fuchs M., Gabel C., Goble A., Goffeau A., Harris D., Hertz-Fowler C., Hilbert H., Horn D., Huang Y., Klages S., Knights A., Kube M., Larke N., Litvin L., Lord A., Louie T., Marra M., Masuy D., Matthews K., Michaeli S., Mottram J.C., Mueller-Auer S., Munden H., Nelson S., Norbertczak H., Oliver K., O'neil S., Pentony M., Pohl T.M., Price C., Purnelle B., Quail M.A., Rabbinowitsch E., Reinhardt R., Rieger M., Rinta J., Robben J., Robertson L., Ruiz J.C., Rutter S., Saunders D., Schaefer M., Schein J., Schwartz D.C., Seeger K., Seyler A., Sharp S., Shin H., Sivam D., Squares R., Squares S., Tosato V., Vogt C., Volckaert G., Wambutt R., Warren T., Wedler H., Woodward J., Zhou S., Zimmermann W., Smith D.F., Blackwell J.M., Stuart K.D., Barrell B.G., Myler P.J.
    Science 309:436-442(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MHOM/IL/81/Friedlin.

Entry informationi

Entry nameiCISY_LEIMA
AccessioniPrimary (citable) accession number: Q4QDX3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: July 19, 2005
Last modified: July 22, 2015
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Miscellaneous

Citrate synthase is found in nearly all cells capable of oxidative metabolism.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.