Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q4Q7X6 (Q4Q7X6_LEIMA) Unreviewed, UniProtKB/TrEMBL

Last modified April 16, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry infoCustomize order

Names and origin

Protein namesRecommended name:
Malate dehydrogenase RuleBase RU003405

EC=1.1.1.37 RuleBase RU003405
Gene names
Name:cMDH EMBL CAJ05744.1
ORF Names:LMJF_28_2860 EMBL CAJ05744.1
OrganismLeishmania major [Reference proteome]
Taxonomic identifier5664 [NCBI]
Taxonomic lineageEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeLeishmaniinaeLeishmania

Protein attributes

Sequence length324 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

(S)-malate + NAD+ = oxaloacetate + NADH. RuleBase RU003405

Sequence similarities

Belongs to the LDH/MDH superfamily. MDH type 2 family. RuleBase RU000421

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Nucleotide binding14 – 152NAD PDB 4I1I
Nucleotide binding128 – 1303NAD PDB 4I1I

Sites

Binding site141Phosphate 1; via amide nitrogen PDB 4I1I
Binding site141Sulfate 1; via amide nitrogen
Binding site251Phosphate 1 PDB 4I1I
Binding site251Phosphate 2 PDB 4I1I
Binding site251Sulfate 2
Binding site251Sulfate 3
Binding site381Sulfate 3
Binding site411NAD PDB 4I1I
Binding site661Sulfate 3
Binding site871NAD PDB 4I1I
Binding site1111NAD PDB 4I1I
Binding site1551NAD; via carbonyl oxygen PDB 4I1I
Binding site1621Phosphate 3 PDB 4I1I
Binding site1621Sulfate 4
Binding site1871NAD PDB 4I1I
Binding site2371Phosphate 3 PDB 4I1I
Binding site2371Sulfate 4

Sequences

Sequence LengthMass (Da)Tools
Q4Q7X6 [UniParc].

Last modified July 19, 2005. Version 1.
Checksum: 0915AD2FF0BC7B67

FASTA32434,155
        10         20         30         40         50         60 
MSAVKVAVTG AAGQIGYALV PLIARGALLG PTTPVELRLL DIEPALKALA GVEAELEDCA 

        70         80         90        100        110        120 
FPLLDKVVVT ADPRVAFDGV AIAIMCGAFP RKAGMERKDL LEMNARIFKE QGEAIAAVAA 

       130        140        150        160        170        180 
SDCRVVVVGN PANTNALILL KSAQGKLNPR HVTAMTRLDH NRALSLLARK AGVPVSQVRN 

       190        200        210        220        230        240 
VIIWGNHSST QVPDTDSAVI GTTPAREAIK DDALDDDFVQ VVRGRGAEII QLRGLSSAMS 

       250        260        270        280        290        300 
AAKAAVDHVH DWIHGTPEGV YVSMGVYSDE NPYGVPSGLI FSFPCTCHAG EWTVVSGKLN 

       310        320 
GDLGKQRLAS TIAELQEERA QAGL 

« Hide

References

« Hide 'large scale' references
[1]"The genome of the kinetoplastid parasite, Leishmania major."
Ivens A.C., Peacock C.S., Worthey E.A., Murphy L., Aggarwal G., Berriman M., Sisk E., Rajandream M.A., Adlem E., Aert R., Anupama A., Apostolou Z., Attipoe P., Bason N., Bauser C., Beck A., Beverley S.M., Bianchettin G. expand/collapse author list , Borzym K., Bothe G., Bruschi C.V., Collins M., Cadag E., Ciarloni L., Clayton C., Coulson R.M.R., Cronin A., Cruz A.K., Davies R.M., De Gaudenzi J., Dobson D.E., Duesterhoeft A., Fazelina G., Fosker N., Frasch A.C., Fraser A., Fuchs M., Gabel C., Goble A., Goffeau A., Harris D., Hertz-Fowler C., Hilbert H., Horn D., Huang Y., Klages S., Knights A., Kube M., Larke N., Litvin L., Lord A., Louie T., Marra M., Masuy D., Matthews K., Michaeli S., Mottram J.C., Mueller-Auer S., Munden H., Nelson S., Norbertczak H., Oliver K., O'neil S., Pentony M., Pohl T.M., Price C., Purnelle B., Quail M.A., Rabbinowitsch E., Reinhardt R., Rieger M., Rinta J., Robben J., Robertson L., Ruiz J.C., Rutter S., Saunders D., Schaefer M., Schein J., Schwartz D.C., Seeger K., Seyler A., Sharp S., Shin H., Sivam D., Squares R., Squares S., Tosato V., Vogt C., Volckaert G., Wambutt R., Warren T., Wedler H., Woodward J., Zhou S., Zimmermann W., Smith D.F., Blackwell J.M., Stuart K.D., Barrell B.G., Myler P.J.
Science 309:436-442(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: MHOM/IL/81/Friedlin.
[2]"Chromosome and gene copy number variation allow major structural change between species and strains of Leishmania."
Rogers M.B., Hilley J.D., Dickens N.J., Wilkes J., Bates P.A., Depledge D.P., Harris D., Her Y., Herzyk P., Imamura H., Otto T.D., Sanders M., Seeger K., Dujardin J.C., Berriman M., Smith D.F., Hertz-Fowler C., Mottram J.C.
Genome Res. 21:2129-2142(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Friedlin EMBL CAJ05744.1.
[3]"Crystal structure of a putative Cytosolic malate dehydrogenase from Leishmania major Friedlin."
Seattle Structural Genomics Center for Infectious Disease (SSGCID)
Abendroth J., Sankaran B., Staker B.L., Stewart L.J., Myler P.
Submitted (SEP-2012) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (1.30 ANGSTROMS) IN COMPLEX WITH SULFATE.
[4]"Crystal structure of a putative Cytosolic malate dehydrogenase from Leishmania major Friedlin in complex with NAD."
SSGCID, Abendroth J., Edwards T.E., Myler P.
Submitted (NOV-2012) to the PDB data bank
Cited for: X-RAY CRYSTALLOGRAPHY (1.50 ANGSTROMS) IN COMPLEX WITH NAD AND PHOSPHATE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
FR796424 Genomic DNA. Translation: CAJ05744.1.
RefSeqXP_001684572.1. XM_001684520.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4H7PX-ray1.30A/B1-324[»]
4I1IX-ray1.50A/B1-324[»]
ProteinModelPortalQ4Q7X6.
SMRQ4Q7X6. Positions 3-320.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsLmjF.28.2860:mRNA; LmjF.28.2860:pep; LmjF.28.2860.
GeneID5653501.
KEGGlma:LMJF_28_2860.

Phylogenomic databases

HOGENOMHOG000220953.
KOK00025.
OMANCLIASK.
ProtClustDBCLSZ2437838.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR23382. PTHR23382. 1 hit.
PfamPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMSSF56327. SSF56327. 1 hit.
TIGRFAMsTIGR01759. MalateDH-SF1. 1 hit.
PROSITEPS00068. MDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameQ4Q7X6_LEIMA
AccessionPrimary (citable) accession number: Q4Q7X6
Entry history
Integrated into UniProtKB/TrEMBL: July 19, 2005
Last sequence update: July 19, 2005
Last modified: April 16, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)