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Q4Q7X6

- Q4Q7X6_LEIMA

UniProt

Q4Q7X6 - Q4Q7X6_LEIMA

Protein

Malate dehydrogenase

Gene

cMDH

Organism
Leishmania major
Status
Unreviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 1 (19 Jul 2005)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    (S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei14 – 141Sulfate 1; via amide nitrogen
    Binding sitei25 – 251Sulfate 2
    Binding sitei25 – 251Sulfate 3
    Binding sitei38 – 381Sulfate 3
    Binding sitei41 – 411NADImported
    Binding sitei66 – 661Sulfate 3
    Binding sitei87 – 871NADImported
    Binding sitei111 – 1111NADImported
    Binding sitei155 – 1551NAD; via carbonyl oxygenImported
    Binding sitei162 – 1621Sulfate 4
    Binding sitei187 – 1871NADImported
    Binding sitei237 – 2371Sulfate 4

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi14 – 152NADImported
    Nucleotide bindingi128 – 1303NADImported

    GO - Molecular functioni

    1. L-malate dehydrogenase activity Source: UniProtKB-EC
    2. nucleotide binding Source: UniProtKB-KW

    GO - Biological processi

    1. cellular carbohydrate metabolic process Source: InterPro
    2. malate metabolic process Source: InterPro
    3. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    OxidoreductaseUniRule annotationImported

    Keywords - Biological processi

    Tricarboxylic acid cycleUniRule annotation

    Keywords - Ligandi

    NADUniRule annotationImported, Nucleotide-bindingImported

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate dehydrogenaseUniRule annotation (EC:1.1.1.37UniRule annotation)
    Gene namesi
    Name:cMDHImported
    ORF Names:LMJF_28_2860Imported
    OrganismiLeishmania majorImported
    Taxonomic identifieri5664 [NCBI]
    Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeLeishmaniinaeLeishmania
    ProteomesiUP000000542: Chromosome 28

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: GeneDB_Lmajor

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4H7PX-ray1.30A/B1-324[»]
    4I1IX-ray1.50A/B1-324[»]
    ProteinModelPortaliQ4Q7X6.
    SMRiQ4Q7X6. Positions 3-320.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the LDH/MDH superfamily. MDH type 2 family.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000220953.
    KOiK00025.
    OMAiNCLIASK.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    InterProiIPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR001252. Malate_DH_AS.
    IPR010945. Malate_DH_type2.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR23382. PTHR23382. 1 hit.
    PfamiPF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
    SUPFAMiSSF56327. SSF56327. 1 hit.
    TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.
    PROSITEiPS00068. MDH. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q4Q7X6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSAVKVAVTG AAGQIGYALV PLIARGALLG PTTPVELRLL DIEPALKALA    50
    GVEAELEDCA FPLLDKVVVT ADPRVAFDGV AIAIMCGAFP RKAGMERKDL 100
    LEMNARIFKE QGEAIAAVAA SDCRVVVVGN PANTNALILL KSAQGKLNPR 150
    HVTAMTRLDH NRALSLLARK AGVPVSQVRN VIIWGNHSST QVPDTDSAVI 200
    GTTPAREAIK DDALDDDFVQ VVRGRGAEII QLRGLSSAMS AAKAAVDHVH 250
    DWIHGTPEGV YVSMGVYSDE NPYGVPSGLI FSFPCTCHAG EWTVVSGKLN 300
    GDLGKQRLAS TIAELQEERA QAGL 324
    Length:324
    Mass (Da):34,155
    Last modified:July 19, 2005 - v1
    Checksum:i0915AD2FF0BC7B67
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FR796424 Genomic DNA. Translation: CAJ05744.1.
    RefSeqiXP_001684572.1. XM_001684520.1.

    Genome annotation databases

    EnsemblProtistsiLmjF.28.2860:mRNA; LmjF.28.2860:pep; LmjF.28.2860.
    GeneIDi5653501.
    KEGGilma:LMJF_28_2860.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FR796424 Genomic DNA. Translation: CAJ05744.1 .
    RefSeqi XP_001684572.1. XM_001684520.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4H7P X-ray 1.30 A/B 1-324 [» ]
    4I1I X-ray 1.50 A/B 1-324 [» ]
    ProteinModelPortali Q4Q7X6.
    SMRi Q4Q7X6. Positions 3-320.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi LmjF.28.2860:mRNA ; LmjF.28.2860:pep ; LmjF.28.2860 .
    GeneIDi 5653501.
    KEGGi lma:LMJF_28_2860.

    Phylogenomic databases

    HOGENOMi HOG000220953.
    KOi K00025.
    OMAi NCLIASK.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    InterProi IPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR001252. Malate_DH_AS.
    IPR010945. Malate_DH_type2.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR23382. PTHR23382. 1 hit.
    Pfami PF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
    SUPFAMi SSF56327. SSF56327. 1 hit.
    TIGRFAMsi TIGR01759. MalateDH-SF1. 1 hit.
    PROSITEi PS00068. MDH. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome of the kinetoplastid parasite, Leishmania major."
      Ivens A.C., Peacock C.S., Worthey E.A., Murphy L., Aggarwal G., Berriman M., Sisk E., Rajandream M.A., Adlem E., Aert R., Anupama A., Apostolou Z., Attipoe P., Bason N., Bauser C., Beck A., Beverley S.M., Bianchettin G.
      , Borzym K., Bothe G., Bruschi C.V., Collins M., Cadag E., Ciarloni L., Clayton C., Coulson R.M.R., Cronin A., Cruz A.K., Davies R.M., De Gaudenzi J., Dobson D.E., Duesterhoeft A., Fazelina G., Fosker N., Frasch A.C., Fraser A., Fuchs M., Gabel C., Goble A., Goffeau A., Harris D., Hertz-Fowler C., Hilbert H., Horn D., Huang Y., Klages S., Knights A., Kube M., Larke N., Litvin L., Lord A., Louie T., Marra M., Masuy D., Matthews K., Michaeli S., Mottram J.C., Mueller-Auer S., Munden H., Nelson S., Norbertczak H., Oliver K., O'neil S., Pentony M., Pohl T.M., Price C., Purnelle B., Quail M.A., Rabbinowitsch E., Reinhardt R., Rieger M., Rinta J., Robben J., Robertson L., Ruiz J.C., Rutter S., Saunders D., Schaefer M., Schein J., Schwartz D.C., Seeger K., Seyler A., Sharp S., Shin H., Sivam D., Squares R., Squares S., Tosato V., Vogt C., Volckaert G., Wambutt R., Warren T., Wedler H., Woodward J., Zhou S., Zimmermann W., Smith D.F., Blackwell J.M., Stuart K.D., Barrell B.G., Myler P.J.
      Science 309:436-442(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: MHOM/IL/81/FriedlinImported.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: FriedlinImported.
    3. "Crystal structure of a putative Cytosolic malate dehydrogenase from Leishmania major Friedlin."
      Seattle Structural Genomics Center for Infectious Disease (SSGCID)
      Abendroth J., Sankaran B., Staker B.L., Stewart L.J., Myler P.
      Submitted (SEP-2012) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (1.30 ANGSTROMS).
    4. "Crystal structure of a putative Cytosolic malate dehydrogenase from Leishmania major Friedlin in complex with NAD."
      SSGCID, Abendroth J., Edwards T.E., Myler P.
      Submitted (NOV-2012) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (1.50 ANGSTROMS) IN COMPLEX WITH NAD.

    Entry informationi

    Entry nameiQ4Q7X6_LEIMA
    AccessioniPrimary (citable) accession number: Q4Q7X6
    Entry historyi
    Integrated into UniProtKB/TrEMBL: July 19, 2005
    Last sequence update: July 19, 2005
    Last modified: October 1, 2014
    This is version 76 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported, Complete proteome, Reference proteomeImported

    External Data

    Dasty 3