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Q4Q7X6

- Q4Q7X6_LEIMA

UniProt

Q4Q7X6 - Q4Q7X6_LEIMA

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Protein

Malate dehydrogenase

Gene

cMDH

Organism
Leishmania major
Status
Unreviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei14 – 141Sulfate 1; via amide nitrogen
Binding sitei25 – 251Sulfate 2
Binding sitei25 – 251Sulfate 3
Binding sitei38 – 381Sulfate 3
Binding sitei41 – 411NADImported
Binding sitei66 – 661Sulfate 3
Binding sitei87 – 871NADImported
Binding sitei111 – 1111NADImported
Binding sitei155 – 1551NAD; via carbonyl oxygenImported
Binding sitei162 – 1621Sulfate 4
Binding sitei187 – 1871NADImported
Binding sitei237 – 2371Sulfate 4

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi14 – 152NADImported
Nucleotide bindingi128 – 1303NADImported

GO - Molecular functioni

  1. L-malate dehydrogenase activity Source: UniProtKB-EC
  2. nucleotide binding Source: UniProtKB-KW

GO - Biological processi

  1. cellular carbohydrate metabolic process Source: InterPro
  2. malate metabolic process Source: InterPro
  3. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

OxidoreductaseUniRule annotation

Keywords - Biological processi

Tricarboxylic acid cycleUniRule annotation

Keywords - Ligandi

NADUniRule annotationImported, Nucleotide-bindingImported

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenaseUniRule annotation (EC:1.1.1.37UniRule annotation)
Gene namesi
Name:cMDHImported
ORF Names:LMJF_28_2860Imported
OrganismiLeishmania majorImported
Taxonomic identifieri5664 [NCBI]
Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeLeishmaniinaeLeishmania
ProteomesiUP000000542: Chromosome 28

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: GeneDB_Lmajor
Complete GO annotation...

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
4H7PX-ray1.30A/B1-324[»]
4I1IX-ray1.50A/B1-324[»]
ProteinModelPortaliQ4Q7X6.
SMRiQ4Q7X6. Positions 3-320.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 2 family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000220953.
InParanoidiQ4Q7X6.
KOiK00025.
OMAiNCLIASK.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23382. PTHR23382. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMiSSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01759. MalateDH-SF1. 1 hit.
PROSITEiPS00068. MDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4Q7X6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSAVKVAVTG AAGQIGYALV PLIARGALLG PTTPVELRLL DIEPALKALA
60 70 80 90 100
GVEAELEDCA FPLLDKVVVT ADPRVAFDGV AIAIMCGAFP RKAGMERKDL
110 120 130 140 150
LEMNARIFKE QGEAIAAVAA SDCRVVVVGN PANTNALILL KSAQGKLNPR
160 170 180 190 200
HVTAMTRLDH NRALSLLARK AGVPVSQVRN VIIWGNHSST QVPDTDSAVI
210 220 230 240 250
GTTPAREAIK DDALDDDFVQ VVRGRGAEII QLRGLSSAMS AAKAAVDHVH
260 270 280 290 300
DWIHGTPEGV YVSMGVYSDE NPYGVPSGLI FSFPCTCHAG EWTVVSGKLN
310 320
GDLGKQRLAS TIAELQEERA QAGL
Length:324
Mass (Da):34,155
Last modified:July 19, 2005 - v1
Checksum:i0915AD2FF0BC7B67
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FR796424 Genomic DNA. Translation: CAJ05744.1.
RefSeqiXP_001684572.1. XM_001684520.1.

Genome annotation databases

EnsemblProtistsiLmjF.28.2860:mRNA; LmjF.28.2860:pep; LmjF.28.2860.
GeneIDi5653501.
KEGGilma:LMJF_28_2860.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
FR796424 Genomic DNA. Translation: CAJ05744.1 .
RefSeqi XP_001684572.1. XM_001684520.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
4H7P X-ray 1.30 A/B 1-324 [» ]
4I1I X-ray 1.50 A/B 1-324 [» ]
ProteinModelPortali Q4Q7X6.
SMRi Q4Q7X6. Positions 3-320.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblProtistsi LmjF.28.2860:mRNA ; LmjF.28.2860:pep ; LmjF.28.2860 .
GeneIDi 5653501.
KEGGi lma:LMJF_28_2860.

Phylogenomic databases

HOGENOMi HOG000220953.
InParanoidi Q4Q7X6.
KOi K00025.
OMAi NCLIASK.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProi IPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR001252. Malate_DH_AS.
IPR010945. Malate_DH_type2.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PANTHERi PTHR23382. PTHR23382. 1 hit.
Pfami PF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
SUPFAMi SSF56327. SSF56327. 1 hit.
TIGRFAMsi TIGR01759. MalateDH-SF1. 1 hit.
PROSITEi PS00068. MDH. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome of the kinetoplastid parasite, Leishmania major."
    Ivens A.C., Peacock C.S., Worthey E.A., Murphy L., Aggarwal G., Berriman M., Sisk E., Rajandream M.A., Adlem E., Aert R., Anupama A., Apostolou Z., Attipoe P., Bason N., Bauser C., Beck A., Beverley S.M., Bianchettin G.
    , Borzym K., Bothe G., Bruschi C.V., Collins M., Cadag E., Ciarloni L., Clayton C., Coulson R.M.R., Cronin A., Cruz A.K., Davies R.M., De Gaudenzi J., Dobson D.E., Duesterhoeft A., Fazelina G., Fosker N., Frasch A.C., Fraser A., Fuchs M., Gabel C., Goble A., Goffeau A., Harris D., Hertz-Fowler C., Hilbert H., Horn D., Huang Y., Klages S., Knights A., Kube M., Larke N., Litvin L., Lord A., Louie T., Marra M., Masuy D., Matthews K., Michaeli S., Mottram J.C., Mueller-Auer S., Munden H., Nelson S., Norbertczak H., Oliver K., O'neil S., Pentony M., Pohl T.M., Price C., Purnelle B., Quail M.A., Rabbinowitsch E., Reinhardt R., Rieger M., Rinta J., Robben J., Robertson L., Ruiz J.C., Rutter S., Saunders D., Schaefer M., Schein J., Schwartz D.C., Seeger K., Seyler A., Sharp S., Shin H., Sivam D., Squares R., Squares S., Tosato V., Vogt C., Volckaert G., Wambutt R., Warren T., Wedler H., Woodward J., Zhou S., Zimmermann W., Smith D.F., Blackwell J.M., Stuart K.D., Barrell B.G., Myler P.J.
    Science 309:436-442(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MHOM/IL/81/FriedlinImported.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: FriedlinImported.
  3. "Crystal structure of a putative Cytosolic malate dehydrogenase from Leishmania major Friedlin."
    Seattle Structural Genomics Center for Infectious Disease (SSGCID)
    Abendroth J., Sankaran B., Staker B.L., Stewart L.J., Myler P.
    Submitted (SEP-2012) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (1.30 ANGSTROMS).
  4. "Crystal structure of a putative Cytosolic malate dehydrogenase from Leishmania major Friedlin in complex with NAD."
    SSGCID, Abendroth J., Edwards T.E., Myler P.
    Submitted (NOV-2012) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (1.50 ANGSTROMS) IN COMPLEX WITH NAD.

Entry informationi

Entry nameiQ4Q7X6_LEIMA
AccessioniPrimary (citable) accession number: Q4Q7X6
Entry historyi
Integrated into UniProtKB/TrEMBL: July 19, 2005
Last sequence update: July 19, 2005
Last modified: October 29, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

3D-structureImported, Complete proteome, Reference proteomeImported

External Data

Dasty 3