Q4Q5S8 (Q4Q5S8_LEIMA) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Glycylpeptide N-tetradecanoyltransferase RuleBase RU000586
EC=2.3.1.97 RuleBase RU000586

Gene names

Name:	NMT EMBL CAJ08448.1
ORF Names:	LMJF_32_0080 EMBL CAJ08448.1

Organism

Leishmania major

Taxonomic identifier

5664 [NCBI]

Taxonomic lineage

Eukaryota › Euglenozoa › Kinetoplastida › Trypanosomatidae › Leishmania

Protein attributes

Sequence length	421 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Adds a myristoyl group to the N-terminal glycine residue of certain cellular proteins By similarity. RuleBase RU000586
Catalytic activity	Tetradecanoyl-CoA + glycylpeptide = CoA + N-tetradecanoylglycylpeptide. RuleBase RU000586
Sequence similarities	Belongs to the NMT family. RuleBase RU004178

Ontologies

Keywords
Molecular function	Acyltransferase RuleBase RU000586 EMBL CAJ08448.1 Transferase
Technical term	3D-structure PDB 2WSA PDB 3H5Z Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	N-terminal protein myristoylation Inferred from electronic annotation. Source: InterPro
Molecular function	glycylpeptide N-tetradecanoyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q4Q5S8 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: E29E51E961B90B44	FASTA	421	48,514
	10 20 30 40 50 60 MSRNPSNSDA AHAFWSTQPV PQTEDETEKI VFAGPMDEPK TVADIPEEPY PIASTFEWWT 70 80 90 100 110 120 PNMEAADDIH AIYELLRDNY VEDDDSMFRF NYSEEFLQWA LCPPNYIPDW HVAVRRKADK 130 140 150 160 170 180 KLLAFIAGVP VTLRMGTPKY MKVKAQEKGE GEEAAKYDEP RHICEINFLC VHKQLREKRL 190 200 210 220 230 240 APILIKEATR RVNRTNVWQA VYTAGVLLPT PYASGQYFHR SLNPEKLVEI RFSGIPAQYQ 250 260 270 280 290 300 KFQNPMAMLK RNYQLPSAPK NSGLREMKPS DVPQVRRILM NYLDSFDVGP VFSDAEISHY 310 320 330 340 350 360 LLPRDGVVFT YVVENDKKVT DFFSFYRIPS TVIGNSNYNL LNAAYVHYYA ATSIPLHQLI 370 380 390 400 410 420 LDLLIVAHSR GFDVCNMVEI LDNRSFVEQL KFGAGDGHLR YYFYNWAYPK IKPSQVALVM L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome of the kinetoplastid parasite, Leishmania major." Ivens A.C., Peacock C.S., Worthey E.A., Murphy L., Aggarwal G., Berriman M., Sisk E., Rajandream M.A., Adlem E., Aert R., Anupama A., Apostolou Z., Attipoe P., Bason N., Bauser C., Beck A., Beverley S.M., Bianchettin G. , Borzym K., Bothe G., Bruschi C.V., Collins M., Cadag E., Ciarloni L., Clayton C., Coulson R.M.R., Cronin A., Cruz A.K., Davies R.M., De Gaudenzi J., Dobson D.E., Duesterhoeft A., Fazelina G., Fosker N., Frasch A.C., Fraser A., Fuchs M., Gabel C., Goble A., Goffeau A., Harris D., Hertz-Fowler C., Hilbert H., Horn D., Huang Y., Klages S., Knights A., Kube M., Larke N., Litvin L., Lord A., Louie T., Marra M., Masuy D., Matthews K., Michaeli S., Mottram J.C., Mueller-Auer S., Munden H., Nelson S., Norbertczak H., Oliver K., O'neil S., Pentony M., Pohl T.M., Price C., Purnelle B., Quail M.A., Rabbinowitsch E., Reinhardt R., Rieger M., Rinta J., Robben J., Robertson L., Ruiz J.C., Rutter S., Saunders D., Schaefer M., Schein J., Schwartz D.C., Seeger K., Seyler A., Sharp S., Shin H., Sivam D., Squares R., Squares S., Tosato V., Vogt C., Volckaert G., Wambutt R., Warren T., Wedler H., Woodward J., Zhou S., Zimmermann W., Smith D.F., Blackwell J.M., Stuart K.D., Barrell B.G., Myler P.J. Science 309:436-442(2005) [PubMed: 16020728] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MHOM/IL/81/Friedlin.
[2]	"N-myristoyltransferase inhibitors as new leads to treat sleeping sickness." Frearson J.A., Brand S., McElroy S.P., Cleghorn L.A., Smid O., Stojanovski L., Price H.P., Guther M.L., Torrie L.S., Robinson D.A., Hallyburton I., Mpamhanga C.P., Brannigan J.A., Wilkinson A.J., Hodgkinson M., Hui R., Qiu W., Raimi O.G. , van Aalten D.M., Brenk R., Gilbert I.H., Read K.D., Fairlamb A.H., Ferguson M.A., Smith D.F., Wyatt P.G. Nature 464:728-732(2010) [PubMed: 20360736] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.49 ANGSTROMS) OF 5-421.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

FR796428 Genomic DNA. Translation: CAJ08448.1.

RefSeq

XP_001685320.1. XM_001685268.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2WSA	X-ray	1.60	A	5-421	[»]
3H5Z	X-ray	1.49	A	5-421	[»]

ProteinModelPortal

Q4Q5S8.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblProtists

LmjF.32.0080:mRNA; LmjF.32.0080:pep; LmjF.32.0080.

GeneID

5656116.

GenomeReviews

Gene locus NMT in contig FR796428_GR.

KEGG

lma:LmjF32.0080.

Organism-specific databases

EuPathDB

EupathDB:LmjF.32.0080.

Phylogenomic databases

HOGENOM

HBG314670.

OMA

EHAKGSP.

PhylomeDB

Q4Q5S8.

ProtClustDB

CLSZ2428978.

Family and domain databases

InterPro

IPR016181. Acyl_CoA_acyltransferase.
IPR000903. MyristoylCoA_TrFase.
IPR022677. MyristoylCoA_TrFase_C.
IPR022678. MyristoylCoA_TrFase_CS.
IPR022676. MyristoylCoA_TrFase_N.
[Graphical view]

Gene3D

G3DSA:3.40.630.30. Acyl_CoA_acyltransferase. 2 hits.

KO

K00671.

PANTHER

PTHR11377. Myristoyl_trans. 1 hit.

Pfam

PF01233. NMT. 2 hits.
PF02799. NMT_C. 1 hit.
[Graphical view]

PIRSF

PIRSF015892. N-myristl_transf. 1 hit.

SUPFAM

SSF55729. Acyl_CoA_acyltransferase. 2 hits.

PROSITE

PS00975. NMT_1. 1 hit.
PS00976. NMT_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

Q4Q5S8_LEIMA

Accession

Primary (citable) accession number: Q4Q5S8

Entry history

Integrated into UniProtKB/TrEMBL:	July 19, 2005
Last sequence update:	July 19, 2005
Last modified:	November 16, 2011
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)