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Q4Q3K2

- Q4Q3K2_LEIMA

UniProt

Q4Q3K2 - Q4Q3K2_LEIMA

Protein
Submitted name:

Ascorbate peroxidase

Gene

APX

Organism
Leishmania major
Status
Unreviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 61 (01 Oct 2014)
      Sequence version 1 (19 Jul 2005)
      Previous versions | rss
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    Functioni

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi69 – 691CalciumImported
    Metal bindingi69 – 691MagnesiumImported
    Metal bindingi72 – 721CalciumImported
    Metal bindingi72 – 721MagnesiumImported
    Metal bindingi81 – 811Calcium; via carbonyl oxygenImported
    Metal bindingi81 – 811Magnesium; via carbonyl oxygenImported
    Metal bindingi86 – 861CalciumImported
    Metal bindingi86 – 861MagnesiumImported
    Metal bindingi92 – 921CalciumImported
    Metal bindingi92 – 921MagnesiumImported
    Metal bindingi192 – 1921Iron (heme axial ligand); via tele nitrogenImported

    GO - Molecular functioni

    1. cytochrome-c oxidase activity Source: GeneDB_Lmajor
    2. heme binding Source: InterPro
    3. iodide peroxidase activity Source: GeneDB_Lmajor
    4. L-ascorbate oxidase activity Source: GeneDB_Lmajor
    5. L-ascorbate peroxidase activity Source: GeneDB_Lmajor
    6. metal ion binding Source: UniProtKB-KW
    7. peroxidase activity Source: GeneDB_Lmajor

    GO - Biological processi

    1. calcium ion homeostasis Source: GeneDB_Lmajor
    2. hydrogen ion transmembrane transport Source: GOC
    3. hydrogen peroxide catabolic process Source: GeneDB_Lmajor
    4. negative regulation of apoptotic process Source: GeneDB_Lmajor
    5. reactive oxygen species metabolic process Source: GeneDB_Lmajor
    6. response to drug Source: GeneDB_Lmajor
    7. response to reactive oxygen species Source: GeneDB_Lmajor
    8. stabilization of membrane potential Source: GeneDB_Lmajor

    Keywords - Molecular functioni

    Oxidoreductase, PeroxidaseImported

    Keywords - Ligandi

    CalciumImported, HemeImported, Iron, MagnesiumImported, Metal-binding

    Protein family/group databases

    PeroxiBasei2334. LmAPx-CcP.

    Names & Taxonomyi

    Protein namesi
    Submitted name:
    Ascorbate peroxidaseImported (EC:1.11.1.11Imported)
    Gene namesi
    Name:APXImported
    ORF Names:LMJF_34_0070Imported
    OrganismiLeishmania majorImported
    Taxonomic identifieri5664 [NCBI]
    Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeLeishmaniinaeLeishmania
    ProteomesiUP000000542: Chromosome 34

    Subcellular locationi

    GO - Cellular componenti

    1. intrinsic component of mitochondrial inner membrane Source: GeneDB_Lmajor

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-60328N.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3RIVX-ray1.76A/B35-303[»]
    3RIWX-ray2.37A/B35-303[»]
    4GEDX-ray1.84A35-301[»]
    ProteinModelPortaliQ4Q3K2.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peroxidase family.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000189824.
    KOiK00434.
    OMAiHTCGECH.

    Family and domain databases

    InterProiIPR002207. Asc_peroxidase.
    IPR010255. Haem_peroxidase.
    IPR002016. Haem_peroxidase_pln/fun/bac.
    IPR019794. Peroxidases_AS.
    [Graphical view]
    PfamiPF00141. peroxidase. 1 hit.
    [Graphical view]
    PRINTSiPR00459. ASPEROXIDASE.
    PR00458. PEROXIDASE.
    SUPFAMiSSF48113. SSF48113. 1 hit.
    PROSITEiPS00436. PEROXIDASE_2. 1 hit.
    PS50873. PEROXIDASE_4. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q4Q3K2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSGTSRRAKG LFTGIAVGTF VSGAMFVSCA SARVEEPPFD IRALRADIED    50
    MISEKLELGP SLIRLAWHEA ASYDCFKKDG SPNSASMRFK PECLYAGNKG 100
    LDIPRKALET LKKKYPQISY ADLWVLAAYV AIEYMGGPTI PFCWGRVDAK 150
    DGSVCGPDGR LPDGSKTQSH VREVFRRLGF NDQETVALIG AHTCGECHIE 200
    FSGYHGPWTH DKNGFDNSFF TQLLDEDWVL NPKVEQMQLM DRATTKLMML 250
    PSDVCLLLDP SYRKYVELYA KDNDRFNKDF ANAFKKLTEL GTRNLHKAPA 300
    SES 303
    Length:303
    Mass (Da):33,873
    Last modified:July 19, 2005 - v1
    Checksum:iB30DB35ACC26E8F0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FR796430 Genomic DNA. Translation: CAJ07706.1.
    RefSeqiXP_001686096.1. XM_001686044.1.

    Genome annotation databases

    EnsemblProtistsiLmjF.34.0070:mRNA; LmjF.34.0070:pep; LmjF.34.0070.
    GeneIDi5654759.
    KEGGilma:LMJF_34_0070.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FR796430 Genomic DNA. Translation: CAJ07706.1 .
    RefSeqi XP_001686096.1. XM_001686044.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3RIV X-ray 1.76 A/B 35-303 [» ]
    3RIW X-ray 2.37 A/B 35-303 [» ]
    4GED X-ray 1.84 A 35-301 [» ]
    ProteinModelPortali Q4Q3K2.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-60328N.

    Protein family/group databases

    PeroxiBasei 2334. LmAPx-CcP.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi LmjF.34.0070:mRNA ; LmjF.34.0070:pep ; LmjF.34.0070 .
    GeneIDi 5654759.
    KEGGi lma:LMJF_34_0070.

    Phylogenomic databases

    HOGENOMi HOG000189824.
    KOi K00434.
    OMAi HTCGECH.

    Family and domain databases

    InterProi IPR002207. Asc_peroxidase.
    IPR010255. Haem_peroxidase.
    IPR002016. Haem_peroxidase_pln/fun/bac.
    IPR019794. Peroxidases_AS.
    [Graphical view ]
    Pfami PF00141. peroxidase. 1 hit.
    [Graphical view ]
    PRINTSi PR00459. ASPEROXIDASE.
    PR00458. PEROXIDASE.
    SUPFAMi SSF48113. SSF48113. 1 hit.
    PROSITEi PS00436. PEROXIDASE_2. 1 hit.
    PS50873. PEROXIDASE_4. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome of the kinetoplastid parasite, Leishmania major."
      Ivens A.C., Peacock C.S., Worthey E.A., Murphy L., Aggarwal G., Berriman M., Sisk E., Rajandream M.A., Adlem E., Aert R., Anupama A., Apostolou Z., Attipoe P., Bason N., Bauser C., Beck A., Beverley S.M., Bianchettin G.
      , Borzym K., Bothe G., Bruschi C.V., Collins M., Cadag E., Ciarloni L., Clayton C., Coulson R.M.R., Cronin A., Cruz A.K., Davies R.M., De Gaudenzi J., Dobson D.E., Duesterhoeft A., Fazelina G., Fosker N., Frasch A.C., Fraser A., Fuchs M., Gabel C., Goble A., Goffeau A., Harris D., Hertz-Fowler C., Hilbert H., Horn D., Huang Y., Klages S., Knights A., Kube M., Larke N., Litvin L., Lord A., Louie T., Marra M., Masuy D., Matthews K., Michaeli S., Mottram J.C., Mueller-Auer S., Munden H., Nelson S., Norbertczak H., Oliver K., O'neil S., Pentony M., Pohl T.M., Price C., Purnelle B., Quail M.A., Rabbinowitsch E., Reinhardt R., Rieger M., Rinta J., Robben J., Robertson L., Ruiz J.C., Rutter S., Saunders D., Schaefer M., Schein J., Schwartz D.C., Seeger K., Seyler A., Sharp S., Shin H., Sivam D., Squares R., Squares S., Tosato V., Vogt C., Volckaert G., Wambutt R., Warren T., Wedler H., Woodward J., Zhou S., Zimmermann W., Smith D.F., Blackwell J.M., Stuart K.D., Barrell B.G., Myler P.J.
      Science 309:436-442(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: MHOM/IL/81/FriedlinImported.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: FriedlinImported.
    3. "Crystal structure of Leishmania major peroxidase and characterization of the compound i tryptophan radical."
      Jasion V.S., Polanco J.A., Meharenna Y.T., Li H., Poulos T.L.
      J. Biol. Chem. 286:24608-24615(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.76 ANGSTROMS) OF 35-303 IN COMPLEX WITH CALCIUM AND HEME.
    4. "Crystal structure of the Leishmania major peroxidase-cytochrome c complex."
      Jasion V.S., Doukov T., Pineda S.H., Li H., Poulos T.L.
      Proc. Natl. Acad. Sci. U.S.A. 109:18390-18394(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.84 ANGSTROMS) OF 35-301 IN COMPLEX WITH HEME AND MAGNESIUM.

    Entry informationi

    Entry nameiQ4Q3K2_LEIMA
    AccessioniPrimary (citable) accession number: Q4Q3K2
    Entry historyi
    Integrated into UniProtKB/TrEMBL: July 19, 2005
    Last sequence update: July 19, 2005
    Last modified: October 1, 2014
    This is version 61 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiUnreviewed (UniProtKB/TrEMBL)

    Miscellaneousi

    Keywords - Technical termi

    3D-structureImported, Complete proteome, Reference proteomeImported

    External Data

    Dasty 3