Ascorbate peroxidase - Leishmania major

Preferred order of sections

Names and origin

Protein names

Submitted name:
Ascorbate peroxidase EMBL CAJ07706.1
EC=1.11.1.11 EMBL CAJ07706.1

Gene names

Name:	APX EMBL CAJ07706.1
ORF Names:	LMJF_34_0070 EMBL CAJ07706.1

Organism

Leishmania major [Reference proteome]

Taxonomic identifier

5664 [NCBI]

Taxonomic lineage

Eukaryota › Euglenozoa › Kinetoplastida › Trypanosomatidae › Leishmaniinae › Leishmania ›

Protein attributes

Sequence length	303 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Sequence similarities

Belongs to the peroxidase family. RuleBase RU004241

Ontologies

Keywords
Ligand	Calcium PDB 3RIW PDB 3RIV Heme PDB 3RIW PDB 3RIV Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase EMBL CAJ07706.1
Technical term	3D-structure PDB 4GED PDB 3RIW PDB 3RIV Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	response to oxidative stress Inferred from electronic annotation. Source: InterPro
Molecular_function	L-ascorbate peroxidase activity Inferred from electronic annotation. Source: EC heme binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Sites

Metal binding

69

1

Calcium PDB 3RIW PDB 3RIV

Metal binding

72

1

Calcium PDB 3RIW PDB 3RIV

Metal binding

81

1

Calcium; via carbonyl oxygen PDB 3RIW PDB 3RIV

Metal binding

86

1

Calcium PDB 3RIW PDB 3RIV

Metal binding

92

1

Calcium PDB 3RIW PDB 3RIV

Metal binding

192

1

Iron (heme axial ligand); via tele nitrogen PDB 3RIW PDB 3RIV

Sequences

Sequence

Length

Mass (Da)

Tools

Q4Q3K2 [UniParc].

Last modified July 19, 2005. Version 1.
Checksum: B30DB35ACC26E8F0
Show »

FASTA

303

33,873

        10         20         30         40         50         60 
MSGTSRRAKG LFTGIAVGTF VSGAMFVSCA SARVEEPPFD IRALRADIED MISEKLELGP 

        70         80         90        100        110        120 
SLIRLAWHEA ASYDCFKKDG SPNSASMRFK PECLYAGNKG LDIPRKALET LKKKYPQISY 

       130        140        150        160        170        180 
ADLWVLAAYV AIEYMGGPTI PFCWGRVDAK DGSVCGPDGR LPDGSKTQSH VREVFRRLGF 

       190        200        210        220        230        240 
NDQETVALIG AHTCGECHIE FSGYHGPWTH DKNGFDNSFF TQLLDEDWVL NPKVEQMQLM 

       250        260        270        280        290        300 
DRATTKLMML PSDVCLLLDP SYRKYVELYA KDNDRFNKDF ANAFKKLTEL GTRNLHKAPA 


SES

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome of the kinetoplastid parasite, Leishmania major." Ivens A.C., Peacock C.S., Worthey E.A., Murphy L., Aggarwal G., Berriman M., Sisk E., Rajandream M.A., Adlem E., Aert R., Anupama A., Apostolou Z., Attipoe P., Bason N., Bauser C., Beck A., Beverley S.M., Bianchettin G. Myler P.J. Science 309:436-442(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MHOM/IL/81/Friedlin.
[2]	"Crystal structure of Leishmania major peroxidase and characterization of the compound i tryptophan radical." Jasion V.S., Polanco J.A., Meharenna Y.T., Li H., Poulos T.L. J. Biol. Chem. 286:24608-24615(2011) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.76 ANGSTROMS) OF 35-303 IN COMPLEX WITH CALCIUM AND HEME.
[3]	"Crystal structure of the Leishmania major peroxidase-cytochrome c complex." Jasion V.S., Doukov T., Pineda S.H., Li H., Poulos T.L. Proc. Natl. Acad. Sci. U.S.A. 109:18390-18394(2012) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.84 ANGSTROMS) OF 35-301.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

FR796430 Genomic DNA. Translation: CAJ07706.1.

RefSeq

XP_001686096.1. XM_001686044.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3RIV	X-ray	1.76	A/B	35-303	[»]
3RIW	X-ray	2.37	A/B	35-303	[»]
4GED	X-ray	1.84	A	35-301	[»]

ProteinModelPortal

Q4Q3K2.

ModBase

Search...

Protein family/group databases

PeroxiBase

2334. LmAPx-CcP.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblProtists

LmjF.34.0070:mRNA; LmjF.34.0070:pep; LmjF.34.0070.

GeneID

5654759.

KEGG

lma:LMJF_34_0070.

Phylogenomic databases

HOGENOM

HOG000189824.

KO

K00434.

OMA

DHGANAG.

ProtClustDB

CLSZ2437495.

Family and domain databases

InterPro

IPR002207. Asc_peroxidase.
IPR010255. Haem_peroxidase.
IPR002016. Haem_peroxidase_pln/fun/bac.
IPR019794. Peroxidases_AS.
[Graphical view]

Pfam

PF00141. peroxidase. 1 hit.
[Graphical view]

PRINTS

PR00459. ASPEROXIDASE.
PR00458. PEROXIDASE.

SUPFAM

SSF48113. Peroxidase_super. 1 hit.

PROSITE

PS00436. PEROXIDASE_2. 1 hit.
PS50873. PEROXIDASE_4. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

Q4Q3K2_LEIMA

Accession

Primary (citable) accession number: Q4Q3K2

Entry history

Integrated into UniProtKB/TrEMBL:	July 19, 2005
Last sequence update:	July 19, 2005
Last modified:	May 1, 2013
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)