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Protein

NAD-dependent protein deacylase SIR2rp3

Gene

SIR2rp3

Organism
Leishmania major
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

NAD-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically removes malonyl, succinyl and glutaryl groups on target proteins. Has weak NAD-dependent protein deacetylase activity; however this activity may not be physiologically relevant in vivo.UniRule annotation

Miscellaneous

This protein may be expected to contain an N-terminal transit peptide but none has been predicted.UniRule annotation

Catalytic activityi

NAD+ + a malonylprotein = nicotinamide + O-malonyl-ADP-ribose + a protein.UniRule annotation
NAD+ + a succinylprotein = nicotinamide + O-succinyl-ADP-ribose + a protein.UniRule annotation
NAD+ + a glutarylprotein = nicotinamide + O-glutaryl-ADP-ribose + a protein.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei56SubstrateUniRule annotation1
Binding sitei59SubstrateUniRule annotation1
Active sitei113Proton acceptorUniRule annotation1
Metal bindingi121ZincUniRule annotation1
Metal bindingi141ZincUniRule annotation1
Binding sitei225NAD; via amide nitrogenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 31NADUniRule annotationAdd BLAST20
Nucleotide bindingi95 – 98NADUniRule annotation4
Nucleotide bindingi181 – 183NADUniRule annotation3

GO - Molecular functioni

Keywordsi

Molecular functionHydrolase
LigandMetal-binding, NAD, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
NAD-dependent protein deacylase SIR2rp3UniRule annotation (EC:3.5.1.-UniRule annotation)
Alternative name(s):
Regulatory protein SIR2 homolog 5UniRule annotation
SIR2-related protein 3
Gene namesi
Name:SIR2rp3
ORF Names:LMJF_34_2140
OrganismiLeishmania major
Taxonomic identifieri5664 [NCBI]
Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeLeishmaniinaeLeishmania
Proteomesi
  • UP000000542 Componenti: Chromosome 34

Subcellular locationi

  • Mitochondrion UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004174251 – 243NAD-dependent protein deacylase SIR2rp3Add BLAST243

Interactioni

Protein-protein interaction databases

STRINGi5664.LmjF.34.2140.

Structurei

3D structure databases

ProteinModelPortaliQ4Q2Y6.
SMRiQ4Q2Y6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 241Deacetylase sirtuin-typeUniRule annotationAdd BLAST241

Domaini

In contrast to class I sirtuins, class III sirtuins have only weak deacetylase activity. Difference in substrate specificity is probably due to a larger hydrophobic pocket with 2 residues (Tyr-56 and Arg-59) that bind to malonylated and succinylated substrates and define the specificity.UniRule annotation

Sequence similaritiesi

Belongs to the sirtuin family. Class III subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG2684. Eukaryota.
COG0846. LUCA.
HOGENOMiHOG000085950.
InParanoidiQ4Q2Y6.
KOiK12410.

Family and domain databases

CDDicd01412. SIRT5_Af1_CobB. 1 hit.
Gene3Di3.40.50.1220. 1 hit.
HAMAPiMF_01121. Sirtuin_ClassIII. 1 hit.
InterProiView protein in InterPro
IPR029035. DHS-like_NAD/FAD-binding_dom.
IPR003000. Sirtuin.
IPR027546. Sirtuin_class_III.
IPR026590. Ssirtuin_cat_dom.
PfamiView protein in Pfam
PF02146. SIR2. 1 hit.
SUPFAMiSSF52467. SSF52467. 1 hit.
PROSITEiView protein in PROSITE
PS50305. SIRTUIN. 1 hit.

Sequencei

Sequence statusi: Complete.

Q4Q2Y6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKACRCITIL TGAGISAESG ISTFRDSNGL WCNHHVEDVA SPDAFIRNPA
60 70 80 90 100
LVQLFYNERR RNLLLSSVKP NKAHTALAKL EEELSGKGKV FIVTQNVDNL
110 120 130 140 150
HERAGSKNVL HMHGELLKAR CTATGNVFEW QKDIVGDVDR CPDCGFLGTL
160 170 180 190 200
RPHIVWFGEM PLCMDEIESI LSTTDLFVAI GTSGNVYPAA GFVKRAQFYG
210 220 230 240
ATTLELNLQE GSNSTLFQES IYGKASSIVP TWVDQVLKES LKK
Length:243
Mass (Da):26,775
Last modified:July 19, 2005 - v1
Checksum:iBADA279EEA864ACD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FR796430 Genomic DNA. Translation: CAJ07927.1.
RefSeqiXP_001686312.1. XM_001686260.1.

Genome annotation databases

EnsemblProtistsiLmjF.34.2140:mRNA; LmjF.34.2140:pep; LmjF.34.2140.
GeneDBiLmjF.34.2140:pep.
GeneIDi5654981.
KEGGilma:LMJF_34_2140.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FR796430 Genomic DNA. Translation: CAJ07927.1.
RefSeqiXP_001686312.1. XM_001686260.1.

3D structure databases

ProteinModelPortaliQ4Q2Y6.
SMRiQ4Q2Y6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi5664.LmjF.34.2140.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblProtistsiLmjF.34.2140:mRNA; LmjF.34.2140:pep; LmjF.34.2140.
GeneDBiLmjF.34.2140:pep.
GeneIDi5654981.
KEGGilma:LMJF_34_2140.

Phylogenomic databases

eggNOGiKOG2684. Eukaryota.
COG0846. LUCA.
HOGENOMiHOG000085950.
InParanoidiQ4Q2Y6.
KOiK12410.

Family and domain databases

CDDicd01412. SIRT5_Af1_CobB. 1 hit.
Gene3Di3.40.50.1220. 1 hit.
HAMAPiMF_01121. Sirtuin_ClassIII. 1 hit.
InterProiView protein in InterPro
IPR029035. DHS-like_NAD/FAD-binding_dom.
IPR003000. Sirtuin.
IPR027546. Sirtuin_class_III.
IPR026590. Ssirtuin_cat_dom.
PfamiView protein in Pfam
PF02146. SIR2. 1 hit.
SUPFAMiSSF52467. SSF52467. 1 hit.
PROSITEiView protein in PROSITE
PS50305. SIRTUIN. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiSIR5_LEIMA
AccessioniPrimary (citable) accession number: Q4Q2Y6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 16, 2012
Last sequence update: July 19, 2005
Last modified: June 7, 2017
This is version 73 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.