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Protein

Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1

Gene

LmjF36.6660

Organism
Leishmania major
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

S-adenosyl-L-methionine-dependent methyltransferase that mediates RNA cap1 2'-O-ribose methylation to the 5'-cap structure of RNAs. Methylates the ribose of the first nucleotide of a m7GpppG-capped mRNA to produce m7GpppNmp (cap1).By similarity

Catalytic activityi

S-adenosyl-L-methionine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(purine-ribonucleotide)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N(7)-methyl 5'-triphosphoguanosine)-(2'-O-methyl-purine-ribonucleotide)-[mRNA].

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei133S-adenosyl-L-methionine; via amide nitrogenPROSITE-ProRule annotation1
Binding sitei212S-adenosyl-L-methioninePROSITE-ProRule annotation1
Active sitei253Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

mRNA capping, mRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1 (EC:2.1.1.57)
Alternative name(s):
Cap1 2'O-ribose methyltransferase 1
Short name:
MTr1
Gene namesi
ORF Names:LmjF36.6660, LmjF_36_6660
OrganismiLeishmania major
Taxonomic identifieri5664 [NCBI]
Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeLeishmaniinaeLeishmania
Proteomesi
  • UP000000542 Componenti: Chromosome 36

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003998041 – 400Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1Add BLAST400

Structurei

3D structure databases

ProteinModelPortaliQ4Q089.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini86 – 299RrmJ-type SAM-dependent 2'-O-MTasePROSITE-ProRule annotationAdd BLAST214

Sequence similaritiesi

Contains 1 RrmJ-type SAM-dependent 2'-O-MTase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3673. Eukaryota.
ENOG410XP08. LUCA.
HOGENOMiHOG000130918.
InParanoidiQ4Q089.
KOiK14589.
OMAiNRAGHKL.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR025816. RrmJ-type_MeTrfase.
IPR002877. rRNA_MeTrfase_FtsJ_dom.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01728. FtsJ. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51613. SAM_MT_RRMJ. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4Q089-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKTVEDRSL EFLIRRRNDA ELPPLASLSC KHLKQHHWRD AFDDEQAALR
60 70 80 90 100
EQLWAVKTQL DVIPAETYTA TRNKLFPLAA SGEQRNFSNR AGHKLLESME
110 120 130 140 150
STGVWMELSK LLRGKSTKRP RDFAFADVCG GPGAFSQALF QAGRKRGWRH
160 170 180 190 200
LHGYGMTLAG VSGLDWYAHL LKSPQFTCTY GLDGTGDIFK LSNIDCLASI
210 220 230 240 250
TKAAPMFLVV ADGGFNVDFS VTNYQETISS RIMYGQWLAA LKLLRKGGCF
260 270 280 290 300
VLKLFDTFSP LSRAVLYLSC CMYRRVHVAK PRHSRVVNSE RYLVCVDFLG
310 320 330 340 350
YPSEGWSRYL DSFYEVGFVD NEHVPQLIPR EWCLQDAVFM ADVRDMNTAV
360 370 380 390 400
ATNQVIALQM ILDAAPAMTE ELKAKTIEKK PAAGSDDGRT PPPDEGGDGE
Length:400
Mass (Da):44,849
Last modified:July 19, 2005 - v1
Checksum:i6C4AB2902636F310
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FR796432 Genomic DNA. Translation: CAJ09646.1.
RefSeqiXP_001687259.1. XM_001687207.1.

Genome annotation databases

GeneIDi5655981.
KEGGilma:LMJF_36_6660.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FR796432 Genomic DNA. Translation: CAJ09646.1.
RefSeqiXP_001687259.1. XM_001687207.1.

3D structure databases

ProteinModelPortaliQ4Q089.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi5655981.
KEGGilma:LMJF_36_6660.

Phylogenomic databases

eggNOGiKOG3673. Eukaryota.
ENOG410XP08. LUCA.
HOGENOMiHOG000130918.
InParanoidiQ4Q089.
KOiK14589.
OMAiNRAGHKL.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR025816. RrmJ-type_MeTrfase.
IPR002877. rRNA_MeTrfase_FtsJ_dom.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01728. FtsJ. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51613. SAM_MT_RRMJ. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMTR1_LEIMA
AccessioniPrimary (citable) accession number: Q4Q089
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: July 19, 2005
Last modified: December 9, 2015
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.