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Protein

Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1

Gene

LmjF36.6660

Organism
Leishmania major
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

S-adenosyl-L-methionine-dependent methyltransferase that mediates RNA cap1 2'-O-ribose methylation to the 5'-cap structure of RNAs. Methylates the ribose of the first nucleotide of a m7GpppG-capped mRNA to produce m7GpppNmp (cap1).By similarity

Catalytic activityi

S-adenosyl-L-methionine + a 5'-(N7-methyl 5'-triphosphoguanosine)-(purine-ribonucleotide)-[mRNA] = S-adenosyl-L-homocysteine + a 5'-(N7-methyl 5'-triphosphoguanosine)-(2'-O-methyl-purine-ribonucleotide)-[mRNA].

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei133S-adenosyl-L-methionine; via amide nitrogenPROSITE-ProRule annotation1
Binding sitei212S-adenosyl-L-methioninePROSITE-ProRule annotation1
Active sitei253Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processmRNA capping, mRNA processing
LigandS-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1 (EC:2.1.1.57)
Alternative name(s):
Cap1 2'O-ribose methyltransferase 1
Short name:
MTr1
Gene namesi
ORF Names:LmjF36.6660, LmjF_36_6660
OrganismiLeishmania major
Taxonomic identifieri5664 [NCBI]
Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeLeishmaniinaeLeishmania
Proteomesi
  • UP000000542 Componenti: Chromosome 36

Organism-specific databases

EuPathDBiTriTrypDB:LmjF.36.6660

Subcellular locationi

GO - Cellular componenti

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003998041 – 400Cap-specific mRNA (nucleoside-2'-O-)-methyltransferase 1Add BLAST400

Interactioni

Protein-protein interaction databases

STRINGi5664.LmjF.36.6660

Structurei

3D structure databases

ProteinModelPortaliQ4Q089
SMRiQ4Q089
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini86 – 299RrmJ-type SAM-dependent 2'-O-MTasePROSITE-ProRule annotationAdd BLAST214

Phylogenomic databases

eggNOGiKOG3673 Eukaryota
ENOG410XP08 LUCA
HOGENOMiHOG000130918
InParanoidiQ4Q089
KOiK14589
OMAiMESTGVW

Family and domain databases

InterProiView protein in InterPro
IPR030376 Cap_mRNA_MeTrfase_1
IPR025816 RrmJ-type_MeTrfase
IPR002877 rRNA_MeTrfase_FtsJ_dom
IPR029063 SAM-dependent_MTases
PANTHERiPTHR16121:SF0 PTHR16121:SF0, 1 hit
PfamiView protein in Pfam
PF01728 FtsJ, 1 hit
SUPFAMiSSF53335 SSF53335, 1 hit
PROSITEiView protein in PROSITE
PS51613 SAM_MT_RRMJ, 1 hit

Sequencei

Sequence statusi: Complete.

Q4Q089-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKTVEDRSL EFLIRRRNDA ELPPLASLSC KHLKQHHWRD AFDDEQAALR
60 70 80 90 100
EQLWAVKTQL DVIPAETYTA TRNKLFPLAA SGEQRNFSNR AGHKLLESME
110 120 130 140 150
STGVWMELSK LLRGKSTKRP RDFAFADVCG GPGAFSQALF QAGRKRGWRH
160 170 180 190 200
LHGYGMTLAG VSGLDWYAHL LKSPQFTCTY GLDGTGDIFK LSNIDCLASI
210 220 230 240 250
TKAAPMFLVV ADGGFNVDFS VTNYQETISS RIMYGQWLAA LKLLRKGGCF
260 270 280 290 300
VLKLFDTFSP LSRAVLYLSC CMYRRVHVAK PRHSRVVNSE RYLVCVDFLG
310 320 330 340 350
YPSEGWSRYL DSFYEVGFVD NEHVPQLIPR EWCLQDAVFM ADVRDMNTAV
360 370 380 390 400
ATNQVIALQM ILDAAPAMTE ELKAKTIEKK PAAGSDDGRT PPPDEGGDGE
Length:400
Mass (Da):44,849
Last modified:July 19, 2005 - v1
Checksum:i6C4AB2902636F310
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FR796432 Genomic DNA Translation: CAJ09646.1
RefSeqiXP_001687259.1, XM_001687207.1

Genome annotation databases

GeneDBiLmjF.36.6660:pep
GeneIDi5655981
KEGGilma:LMJF_36_6660

Similar proteinsi

Entry informationi

Entry nameiMTR1_LEIMA
AccessioniPrimary (citable) accession number: Q4Q089
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: July 19, 2005
Last modified: April 25, 2018
This is version 58 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health