Reviewed, UniProtKB/Swiss-Prot Q4PZA2 (ECE1_MOUSE)

Names and origin

Protein names

Recommended name:
    Endothelin-converting enzyme 1
      Short name=ECE-1
    EC=3.4.24.71

Gene names

Name:

Ece1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	769 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Converts big endothelin-1 to endothelin-1 By similarity.
Catalytic activity	Hydrolysis of the 21-Trp-|-Val-22 bond in big endothelin to form endothelin 1.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Enzyme regulation	Inhibited by phosphoramidon By similarity.
Subunit structure	Homodimer; disulfide-linked By similarity.
Subcellular location	Cell membrane; Single-pass type II membrane protein By similarity.
Sequence similarities	Belongs to the peptidase M13 family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Domain	Signal-anchor Transmembrane
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
PTM	Disulfide bond Glycoprotein Phosphoprotein
Gene Ontology (GO)
Biological process	pharyngeal system development Inferred from mutant phenotype. Source: MGI proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 4 isoforms produced by alternative splicing. [Align] [Select]

Isoform B (identifier: Q4PZA2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform A (identifier: Q4PZA2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-43: MRTVWSPLAAALAALGMSTYKRATLDEEDLVDSLSEGDVYPNG → MPPQSLGLQRGSFFLGKRGPGLMVSLPLLASS

Isoform C (identifier: Q4PZA2-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-16: Missing.

Isoform D (identifier: Q4PZA2-4)

The sequence of this isoform differs from the canonical sequence as follows:
     1-16: MRTVWSPLAAALAALG → METLRESVLHLALQ

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 769	769	Endothelin-converting enzyme 1		PRO_0000240629
Regions
	Topological domain	1 – 67	67	Cytoplasmic Potential
	Transmembrane	68 – 88	21	Signal-anchor for type II membrane protein Potential
	Topological domain	89 – 769	681	Extracellular Potential
Sites
	Active site	607	1	By similarity
	Active site	670	1	Proton donor By similarity
	Metal binding	606	1	Zinc; catalytic By similarity
	Metal binding	610	1	Zinc; catalytic By similarity
	Metal binding	666	1	Zinc; catalytic By similarity
Amino acid modifications
	Modified residue	33	1	Phosphoserine By similarity
	Glycosylation	165	1	N-linked (GlcNAc...) Potential
	Glycosylation	186	1	N-linked (GlcNAc...) Potential
	Glycosylation	209	1	N-linked (GlcNAc...) Potential
	Glycosylation	269	1	N-linked (GlcNAc...) Potential
	Glycosylation	315	1	N-linked (GlcNAc...) Potential
	Glycosylation	361	1	N-linked (GlcNAc...) Potential
	Glycosylation	382	1	N-linked (GlcNAc...) Potential
	Glycosylation	538	1	N-linked (GlcNAc...) Potential
	Glycosylation	631	1	N-linked (GlcNAc...) Potential
	Glycosylation	650	1	N-linked (GlcNAc...) Potential
	Disulfide bond	427		Interchain By similarity
Natural variations
	Alternative sequence	1 – 43	43	MRTVW…VYPNG → MPPQSLGLQRGSFFLGKRGP GLMVSLPLLASS in isoform A.		VSP_019399
	Alternative sequence	1 – 16	16	Missing in isoform C.		VSP_019400
	Alternative sequence	1 – 16	16	MRTVW…LAALG → METLRESVLHLALQ in isoform D.		VSP_019401

Sequences

	Sequence		Length	Mass (Da)
	Isoform B [UniParc]. Last modified July 19, 2005. Version 1. Checksum: 58214938A0F2760A	FASTA	769	87,073
	10 20 30 40 50 60 MRTVWSPLAA ALAALGMSTY KRATLDEEDL VDSLSEGDVY PNGLQVNFRS SRSGQRCWAA 70 80 90 100 110 120 RTSVEKRLVV LVTLLAAGLV ACLAALGIQY QTRTPPVCLT EACVSVTSSI LNSMDPTVDP 130 140 150 160 170 180 CQDFFSYACG GWIKANPVPD GHSRWGTFSN LWEHNQAVIK HLLENATASV SEAERKAQVY 190 200 210 220 230 240 YRACMNETRI EELRAKPLME LIEKLGGWNI TGPWAKDNFQ DTLQVVTAHY RTSPFFSVYV 250 260 270 280 290 300 SADSKNSNSN VIQVDQSGLG LPSRDYYLNK TENEKVLTGY LNYMVQLGKL LGGGDEDAIR 310 320 330 340 350 360 PQMQQILDFE TALANITIPQ EKRRDEELIY HKVTAAELQT LAPAINWLPF LNTIFYPVEI 370 380 390 400 410 420 NESEPIVVYD KEYLRQVSTL INNTDKCLLN NYMMWNLVRK TSSFLDQRFQ DADEKFMEVM 430 440 450 460 470 480 YGTKKTCIPR WKFCVSDTEN NLGFALGPMF VKATFAEDSK NIASEIIMEI KKAFEESLST 490 500 510 520 530 540 LKWMDEETRR SAKEKADAIY NMIGYPNFIM DPKELDKVFN DYTAVPDLYF ENAMRFFNFS 550 560 570 580 590 600 WRVTADQLRK APNRDQWSMT PPMVNAYYSP TKNEIVFPAG ILQAPFYTRS SPNALNFGGI 610 620 630 640 650 660 GVVVGHELTH AFDDQGREYD KDGNLRPWWK NSSVEAFKQQ TECMVQQYSN YSVNGEPVNG 670 680 690 700 710 720 RHTLGENIAD NGGLKAAYRA YQNWVKKNGA EQTLPTLGLT SNQLFFLGFA QVWCSVRTPE 730 740 750 760 SSHEGLITDP HSPSRFRVIG SLSNSKEFSE HFRCPPGSPM NPHHKCEVW
	Isoform A. Checksum: 0CC66DD2DEAE206D	FASTA	758	85,800
	10 20 30 40 50 60 MPPQSLGLQR GSFFLGKRGP GLMVSLPLLA SSLQVNFRSS RSGQRCWAAR TSVEKRLVVL 70 80 90 100 110 120 VTLLAAGLVA CLAALGIQYQ TRTPPVCLTE ACVSVTSSIL NSMDPTVDPC QDFFSYACGG 130 140 150 160 170 180 WIKANPVPDG HSRWGTFSNL WEHNQAVIKH LLENATASVS EAERKAQVYY RACMNETRIE 190 200 210 220 230 240 ELRAKPLMEL IEKLGGWNIT GPWAKDNFQD TLQVVTAHYR TSPFFSVYVS ADSKNSNSNV 250 260 270 280 290 300 IQVDQSGLGL PSRDYYLNKT ENEKVLTGYL NYMVQLGKLL GGGDEDAIRP QMQQILDFET 310 320 330 340 350 360 ALANITIPQE KRRDEELIYH KVTAAELQTL APAINWLPFL NTIFYPVEIN ESEPIVVYDK 370 380 390 400 410 420 EYLRQVSTLI NNTDKCLLNN YMMWNLVRKT SSFLDQRFQD ADEKFMEVMY GTKKTCIPRW 430 440 450 460 470 480 KFCVSDTENN LGFALGPMFV KATFAEDSKN IASEIIMEIK KAFEESLSTL KWMDEETRRS 490 500 510 520 530 540 AKEKADAIYN MIGYPNFIMD PKELDKVFND YTAVPDLYFE NAMRFFNFSW RVTADQLRKA 550 560 570 580 590 600 PNRDQWSMTP PMVNAYYSPT KNEIVFPAGI LQAPFYTRSS PNALNFGGIG VVVGHELTHA 610 620 630 640 650 660 FDDQGREYDK DGNLRPWWKN SSVEAFKQQT ECMVQQYSNY SVNGEPVNGR HTLGENIADN 670 680 690 700 710 720 GGLKAAYRAY QNWVKKNGAE QTLPTLGLTS NQLFFLGFAQ VWCSVRTPES SHEGLITDPH 730 740 750 SPSRFRVIGS LSNSKEFSEH FRCPPGSPMN PHHKCEVW
	Isoform C. Checksum: AEEF9C11ABB6657C	FASTA	753	85,463
	10 20 30 40 50 60 MSTYKRATLD EEDLVDSLSE GDVYPNGLQV NFRSSRSGQR CWAARTSVEK RLVVLVTLLA 70 80 90 100 110 120 AGLVACLAAL GIQYQTRTPP VCLTEACVSV TSSILNSMDP TVDPCQDFFS YACGGWIKAN 130 140 150 160 170 180 PVPDGHSRWG TFSNLWEHNQ AVIKHLLENA TASVSEAERK AQVYYRACMN ETRIEELRAK 190 200 210 220 230 240 PLMELIEKLG GWNITGPWAK DNFQDTLQVV TAHYRTSPFF SVYVSADSKN SNSNVIQVDQ 250 260 270 280 290 300 SGLGLPSRDY YLNKTENEKV LTGYLNYMVQ LGKLLGGGDE DAIRPQMQQI LDFETALANI 310 320 330 340 350 360 TIPQEKRRDE ELIYHKVTAA ELQTLAPAIN WLPFLNTIFY PVEINESEPI VVYDKEYLRQ 370 380 390 400 410 420 VSTLINNTDK CLLNNYMMWN LVRKTSSFLD QRFQDADEKF MEVMYGTKKT CIPRWKFCVS 430 440 450 460 470 480 DTENNLGFAL GPMFVKATFA EDSKNIASEI IMEIKKAFEE SLSTLKWMDE ETRRSAKEKA 490 500 510 520 530 540 DAIYNMIGYP NFIMDPKELD KVFNDYTAVP DLYFENAMRF FNFSWRVTAD QLRKAPNRDQ 550 560 570 580 590 600 WSMTPPMVNA YYSPTKNEIV FPAGILQAPF YTRSSPNALN FGGIGVVVGH ELTHAFDDQG 610 620 630 640 650 660 REYDKDGNLR PWWKNSSVEA FKQQTECMVQ QYSNYSVNGE PVNGRHTLGE NIADNGGLKA 670 680 690 700 710 720 AYRAYQNWVK KNGAEQTLPT LGLTSNQLFF LGFAQVWCSV RTPESSHEGL ITDPHSPSRF 730 740 750 RVIGSLSNSK EFSEHFRCPP GSPMNPHHKC EVW
	Isoform D. Checksum: 54118EC09E36D032	FASTA	767	87,085
	10 20 30 40 50 60 METLRESVLH LALQMSTYKR ATLDEEDLVD SLSEGDVYPN GLQVNFRSSR SGQRCWAART 70 80 90 100 110 120 SVEKRLVVLV TLLAAGLVAC LAALGIQYQT RTPPVCLTEA CVSVTSSILN SMDPTVDPCQ 130 140 150 160 170 180 DFFSYACGGW IKANPVPDGH SRWGTFSNLW EHNQAVIKHL LENATASVSE AERKAQVYYR 190 200 210 220 230 240 ACMNETRIEE LRAKPLMELI EKLGGWNITG PWAKDNFQDT LQVVTAHYRT SPFFSVYVSA 250 260 270 280 290 300 DSKNSNSNVI QVDQSGLGLP SRDYYLNKTE NEKVLTGYLN YMVQLGKLLG GGDEDAIRPQ 310 320 330 340 350 360 MQQILDFETA LANITIPQEK RRDEELIYHK VTAAELQTLA PAINWLPFLN TIFYPVEINE 370 380 390 400 410 420 SEPIVVYDKE YLRQVSTLIN NTDKCLLNNY MMWNLVRKTS SFLDQRFQDA DEKFMEVMYG 430 440 450 460 470 480 TKKTCIPRWK FCVSDTENNL GFALGPMFVK ATFAEDSKNI ASEIIMEIKK AFEESLSTLK 490 500 510 520 530 540 WMDEETRRSA KEKADAIYNM IGYPNFIMDP KELDKVFNDY TAVPDLYFEN AMRFFNFSWR 550 560 570 580 590 600 VTADQLRKAP NRDQWSMTPP MVNAYYSPTK NEIVFPAGIL QAPFYTRSSP NALNFGGIGV 610 620 630 640 650 660 VVGHELTHAF DDQGREYDKD GNLRPWWKNS SVEAFKQQTE CMVQQYSNYS VNGEPVNGRH 670 680 690 700 710 720 TLGENIADNG GLKAAYRAYQ NWVKKNGAEQ TLPTLGLTSN QLFFLGFAQV WCSVRTPESS 730 740 750 760 HEGLITDPHS PSRFRVIGSL SNSKEFSEHF RCPPGSPMNP HHKCEVW

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genomic organisation of the mouse gene encoding endothelin-converting enzyme-1 (ECE-1) and mRNA expression of ECE-1 isoforms in murine tissues." Lindenau S., von Langsdorff C., Saxena A., Paul M., Orzechowski H.-D. Gene 373:109-115(2006) [PubMed: 16540265] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING. Strain: BALB/c.
[2]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM C). Strain: C57BL/6J. Tissue: Thymus.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM C). Strain: C57BL/6. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	DQ022741 , DQ022723, DQ022725, DQ022727, DQ022726, DQ022728, DQ022730, DQ022732, DQ022734, DQ022736, DQ022740, DQ022739, DQ022738, DQ022737, DQ022735, DQ022733, DQ022731, DQ022729 Genomic DNA. Translation: AAY81993.1. DQ022741 , DQ022721, DQ022723, DQ022725, DQ022726, DQ022728, DQ022730, DQ022732, DQ022734, DQ022736, DQ022740, DQ022739, DQ022738, DQ022737, DQ022735, DQ022733, DQ022731, DQ022729, DQ022727 Genomic DNA. Translation: AAY81995.1. DQ022741 , DQ022722, DQ022723, DQ022725, DQ022726, DQ022728, DQ022730, DQ022732, DQ022734, DQ022736, DQ022740, DQ022739, DQ022738, DQ022737, DQ022735, DQ022733, DQ022731, DQ022729, DQ022727 Genomic DNA. Translation: AAY81996.1. DQ022741 , DQ022724, DQ022725, DQ022727, DQ022726, DQ022728, DQ022730, DQ022732, DQ022734, DQ022736, DQ022740, DQ022739, DQ022738, DQ022737, DQ022735, DQ022733, DQ022731, DQ022729 Genomic DNA. Translation: AAY81997.1. AK134088 mRNA. Translation: BAE22007.1. BC060648 mRNA. Translation: AAH60648.1.
IPI	IPI00396840. IPI00648026. IPI00762055. IPI00762815.
RefSeq	NP_955011.1.
UniGene	Mm.401062 Mm.439998
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	M13.002.
PTM databases
PhosphoSite	Q4PZA2.
Proteomic databases
PRIDE	Q4PZA2.
Genome annotation databases
Ensembl	ENSMUSG00000057530. Mus musculus. [Contig view]
GeneID	230857.
KEGG	mmu:230857.
Organism-specific databases
MGI	MGI:1101357. Ece1.
Phylogenomic databases
HOVERGEN	Q4PZA2.
OMA	Q4PZA2. MDELVAN.
Enzyme and pathway databases
BRENDA	3.4.24.71. 244.
Gene expression databases
ArrayExpress	Q4PZA2.
Bgee	Q4PZA2.
CleanEx	MM_ECE1.
GermOnline	ENSMUSG00000057530. Mus musculus.
Family and domain databases
InterPro	IPR006025. Pept_M_Zn_BS. IPR000718. Peptidase_M13. IPR018497. Peptidase_M13_C. IPR008753. Peptidase_M13_N. [Graphical view]
PANTHER	PTHR11733. Peptidase_M13. 1 hit.
Pfam	PF01431. Peptidase_M13. 1 hit. PF05649. Peptidase_M13_N. 1 hit. [Graphical view]
PRINTS	PR00786. NEPRILYSIN.
PROSITE	PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	380214.
SOURCE	Search...

Entry information

Entry name

ECE1_MOUSE

Accession

Primary (citable) accession number: Q4PZA2
Secondary accession number(s): Q4PZ99, Q4PZA1, Q6P9Q9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2006
Last sequence update:	July 19, 2005
Last modified:	June 16, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)