Reviewed, UniProtKB/Swiss-Prot Q4PT34 (PME56_ARATH)

Names and origin

Protein names

Recommended name:
    Probable pectinesterase 56
      Short name=PE 56
    EC=3.1.1.11
Alternative name(s):
    Pectin methylesterase 56
      Short name=AtPME56

Gene names

Name:	PME56
Synonyms:	ARATH56
Ordered Locus Names:	At1g11370
ORF Names:	T23J18.3

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	288 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Acts in the modification of cell walls via demethylesterification of cell wall pectin By similarity.
Catalytic activity	Pectin + n H2O = n methanol + pectate.
Pathway	Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.
Subcellular location	Secreted › cell wall By similarity.
Sequence similarities	Belongs to the pectinesterase family.
Sequence caution	The sequence AAF16649.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell wall Secreted
Domain	Signal
Molecular function	Aspartyl esterase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell wall modification Inferred from electronic annotation. Source: InterPro
Cellular component	cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	aspartyl esterase activity Inferred from electronic annotation. Source: UniProtKB-KW pectinesterase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Potential
	Chain	28 – 288	261	Probable pectinesterase 56		PRO_0000371704
Sites
	Active site	173	1	Proton donor By similarity
	Active site	194	1	Nucleophile By similarity
	Binding site	120	1	Substrate By similarity
	Binding site	150	1	Substrate By similarity
	Binding site	262	1	Substrate By similarity
	Binding site	264	1	Substrate By similarity
	Site	172	1	Transition state stabilizer By similarity
Amino acid modifications
	Glycosylation	55	1	N-linked (GlcNAc...) Potential
	Glycosylation	95	1	N-linked (GlcNAc...) Potential
	Glycosylation	242	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q4PT34-1 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: 0643EEF1B0EE2549	FASTA	288	31,400
	10 20 30 40 50 60 MAMTSTMQLL VLSFLVIASL FLGATVAPPA SLISTPDQAL KDKADLIVAK DGSGNFTTVN 70 80 90 100 110 120 EAVAAAPENG VKPFVIYIKE GLYKEVIRIG KKKTNLTLVG DGRDLTVLSG DLNGVDGIKT 130 140 150 160 170 180 FDSATLAVDE SGFMAQDLCI RNTAGPEKRQ AVALRISTDM TIIYRCRIDA YQDTLYAYSG 190 200 210 220 230 240 RQFYRDCYIT GTVDFIFGRA AAVFQYCQIE ARKPGIGQTN ILTAQSREED TATSGFSFQK 250 260 270 280 CNISASSDLT PIKGTVKTFL GRPWRAFSRV VFMESFIDDV IDRAGWTP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W., Redman J.C., Wu H.C., Utterback T., Town C.D. Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"Pectin methylesterases: sequence-structural features and phylogenetic relationships." Markovic O., Janecek S. Carbohydr. Res. 339:2281-2295(2004) [PubMed: 15337457] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC011661 Genomic DNA. Translation: AAF16649.1. Sequence problems. DQ056452 mRNA. Translation: AAY78609.1.
IPI	IPI00619846.
PIR	F86247.
RefSeq	NP_172604.1.
UniGene	At.51567
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	837679.
GenomeReviews	Gene locus AT1G11370 in contig CT485782_GR.
KEGG	ath:AT1G11370.
NMPDR	fig|3702.1.peg.1390.
Organism-specific databases
TAIR	At1g11370.
Phylogenomic databases
OMA	VALRIST.
Gene expression databases
Genevestigator	Q4PT34.
Family and domain databases
InterPro	IPR012334. Pectin_lyas_fold. IPR018040. Pectinesterase_AS. IPR000070. Pectinesterase_cat. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF01095. Pectinesterase. 1 hit. [Graphical view]
PROSITE	PS00800. PECTINESTERASE_1. False negative. PS00503. PECTINESTERASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PME56_ARATH

Accession

Primary (citable) accession number: Q4PT34
Secondary accession number(s): Q9LPZ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	July 19, 2005
Last modified:	November 3, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)