Reviewed, UniProtKB/Swiss-Prot Q4PSU4 (AGL61_ARATH)

Names and origin

Protein names

Recommended name:
    Agamous-like MADS-box protein AGL61
Alternative name(s):
    Protein DIANA

Gene names

Name:	AGL61
Synonyms:	DIA
Ordered Locus Names:	At2g24840
ORF Names:	F27C12.24

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	264 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Probable transcription factor. Controls central cell differentiation during female gametophyte development. Ref.3 Ref.4
Subunit structure	Interacts with PHE1/AGL37, PHE2/AGL38, AGL80 and AGL86. Forms a heterodimer with AGL80. Ref.3 Ref.4
Subcellular location	Nucleus. Note: The transport to the nucleus is dependent on dimerization with AGL80. Ref.3
Tissue specificity	Expressed exclusively in the central cell of the female gametophyte and in early endosperm. Ref.3 Ref.4
Developmental stage	Expressed in the final stages of embryo sac development. Ref.4
Miscellaneous	Plants lacking AGL61 show a reduced size of the central cell and a reduced or absent central cell vacuole. When fertilized, the central cell fails to give rise to endosperm and the embryo sac degenerates. However, mutated female gametophytesol are not defective in pollen tube guidance and the synergid function is not impaired. This protein was called 'DIANA' after the virginal Roman goddess of the hunt.
Sequence similarities	Contains 1 MADS-box domain.
Sequence caution	The sequence AAD23028.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	DNA-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	embryo sac central cell differentiation Ref.3 Inferred from mutant phenotype. Source: TAIR regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Ref.3 Inferred from direct assay. Source: TAIR
Molecular function	protein binding Ref.4 Inferred from physical interaction. Source: IntAct sequence-specific DNA binding Inferred from electronic annotation. Source: InterPro transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	Q2V412	1	EBI-1237976,EBI-1238781
AGL80	Q9FJK3	2	EBI-1237976,EBI-622424
CALM	P62157	1	EBI-1237976,EBI-397403	From a different organism.
CAM1	P25854	1	EBI-1237976,EBI-1235664
CAM6	Q03509	1	EBI-1237976,EBI-1236097
CAM7	P59220	1	EBI-1237976,EBI-1236031
CML10	P30187	1	EBI-1237976,EBI-1238722
CML8	O23320	1	EBI-1237976,EBI-1237764
CML9	Q9S744	1	EBI-1237976,EBI-1236048

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 264	264	Agamous-like MADS-box protein AGL61		PRO_0000363649
Regions
	Domain	62 – 122	61	MADS-box

Sequences

	Sequence		Length	Mass (Da)
	Q4PSU4-1 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: 517BACA6BF7B4CDC	FASTA	264	30,264
	10 20 30 40 50 60 MYVTKYKNKL PTLQVDLIIM PHQTQACIYK QTLSLHQIFQ QRKATKTLHT KKTMMSKKKE 70 80 90 100 110 120 SIGRQKIPMV KIKKESHRQV TFSKRRAGLF KKASELCTLC GAEIGIIVFS PAKKPFSFGH 130 140 150 160 170 180 PSVESVLDRY VSRNNMSLAQ SQQLQGSPAA SCELNMQLTH ILSEVEEEKK KGQAMEEMRK 190 200 210 220 230 240 ESVRRSMINW WEKPVEEMNM VQLQEMKYAL EELRKTVVTN MASFNEAKDD VFGFLDNKVT 250 260 VPPYVNMPSG PSNIYNFANG NGCF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W., Redman J.C., Wu H.C., Utterback T., Town C.D. Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"AGL61 interacts with AGL80 and is required for central cell development in Arabidopsis." Steffen J.G., Kang I.-H., Portereiko M.F., Lloyd A., Drews G.N. Plant Physiol. 148:259-268(2008) [PubMed: 18599653] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 39-264, FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH AGL80.
[4]	"The MADS domain protein DIANA acts together with AGAMOUS-LIKE80 to specify the central cell in Arabidopsis ovules." Bemer M., Wolters-Arts M., Grossniklaus U., Angenent G.C. Plant Cell 20:2088-2101(2008) [PubMed: 18713950] [Abstract] Cited for: FUNCTION, INTERACTION WITH AGL80; AGL86; PHE1 AND PHE2, DEVELOPMENTAL STAGE, TISSUE SPECIFICITY.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AC006585 Genomic DNA. Translation: AAD23028.1. Sequence problems. DQ056542 mRNA. Translation: AAY78694.1. EU836691 mRNA. Translation: ACF37253.1. Different initiation.
IPI	IPI00545091.
PIR	B84641.
RefSeq	NP_850058.1.
UniGene	At.52900
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q4PSU4. 12 interactions.
Genome annotation databases
GeneID	817021.
GenomeReviews	Gene locus AT2G24840 in contig CT485783_GR.
KEGG	ath:AT2G24840.
Organism-specific databases
GeneFarm	3717. 371.
TAIR	At2g24840.
Family and domain databases
InterPro	IPR002100. TF_MADSbox. [Graphical view]
Pfam	PF00319. SRF-TF. 1 hit. [Graphical view]
PRINTS	PR00404. MADSDOMAIN.
SMART	SM00432. MADS. 1 hit. [Graphical view]
PROSITE	PS00350. MADS_BOX_1. False negative. PS50066. MADS_BOX_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AGL61_ARATH

Accession

Primary (citable) accession number: Q4PSU4
Secondary accession number(s): Q9SK48

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	July 19, 2005
Last modified:	June 16, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)