Q4PSQ5 (PME66_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable pectinesterase 66
Short name=PE 66
EC=3.1.1.11

Alternative name(s):
Pectin methylesterase 66
Short name=AtPME66

Gene names

Name:	PME66
Synonyms:	ARATH66
Ordered Locus Names:	At2g47280
ORF Names:	T8I13.12

Organism

Arabidopsis thaliana (Mouse-ear cress)

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Acts in the modification of cell walls via demethylesterification of cell wall pectin By similarity.
Catalytic activity	Pectin + n H2O = n methanol + pectate.
Pathway	Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.
Subcellular location	Secreted › cell wall By similarity.
Sequence similarities	Belongs to the pectinesterase family.
Sequence caution	The sequence AAB63828.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell wall Secreted
Domain	Signal
Molecular function	Aspartyl esterase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell wall modification Inferred from electronic annotation. Source: InterPro cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	aspartyl esterase activity Inferred from electronic annotation. Source: UniProtKB-KW pectinesterase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27	Potential
	Chain	28 – 336	309	Probable pectinesterase 66		PRO_0000371710
Sites
	Active site	164	1	Proton donor By similarity
	Active site	185	1	Nucleophile By similarity
	Binding site	247	1	Substrate By similarity
	Binding site	249	1	Substrate By similarity
	Site	163	1	Transition state stabilizer By similarity
Amino acid modifications
	Glycosylation	44	1	N-linked (GlcNAc...) Potential
	Glycosylation	201	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q4PSQ5 [UniParc]. Last modified May 5, 2009. Version 2. Checksum: 5041C5F58A49939C	FASTA	336	37,017
	10 20 30 40 50 60 MKRVASYNYK IVCMVVMTLF VYGYSAAAEQ IAYTITVDLN GGGNFTTVQS AIDSISPPNH 70 80 90 100 110 120 NWIRVFTQNG IYREKVTIPK EKGFIYLQGK GIEQTVIEYD DHQATDISAT FTAFADDIVI 130 140 150 160 170 180 SGITFKNTYN IVPNNKREIV PAVAARMLGD RYVVTDSSFV GLQDTLFDGK GRHYYKRCII 190 200 210 220 230 240 SGGIDFIFGY GQSLFKECTL NMTLGIYAPD NPYGTITAHQ RPSPSDEGGF VFSDCTVTGV 250 260 270 280 290 300 GKTLLGRAWG SNARVIFDRS RLSDVVLPIG WDAWRAKGNE RDLTFVEAGC TGAGADTSQR 310 320 330 VPWLKKLSLS EVDGFASVSF IDQDGWISRF PIEGGP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W., Redman J.C., Wu H.C., Utterback T., Town C.D. Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 17-336. Strain: cv. Columbia.
[4]	"Pectin methylesterases: sequence-structural features and phylogenetic relationships." Markovic O., Janecek S. Carbohydr. Res. 339:2281-2295(2004) [PubMed: 15337457] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC002337 Genomic DNA. Translation: AAB63828.1. Sequence problems. CP002685 Genomic DNA. Translation: AEC10823.1. DQ056581 mRNA. Translation: AAY78730.1.
IPI	IPI00518143.
PIR	C84913.
RefSeq	NP_850471.2. NM_180140.2.
UniGene	At.53144.
3D structure databases
HSSP	HSSP built from PDB template 1GQ8 based on UniProtKB P83218.
ProteinModelPortal	Q4PSQ5.
SMR	Q4PSQ5. Positions 28-335.
ModBase	Search...
Proteomic databases
PRIDE	Q4PSQ5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT2G47280.1; AT2G47280.1; AT2G47280.
GeneID	819341.
GenomeReviews	Gene locus AT2G47280 in contig CT485783_GR.
KEGG	ath:AT2G47280.
NMPDR	fig|3702.1.peg.11915.
Organism-specific databases
TAIR	At2g47280.
Phylogenomic databases
eggNOG	COG4677.
GeneTree	EPGT00070000028060.
HOGENOM	HBG747179.
InParanoid	Q4PSQ5.
PhylomeDB	Q4PSQ5.
ProtClustDB	PLN02176.
Gene expression databases
Genevestigator	Q4PSQ5.
Family and domain databases
InterPro	IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. IPR000070. Pectinesterase_cat. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF01095. Pectinesterase. 1 hit. [Graphical view]
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit.
PROSITE	PS00800. PECTINESTERASE_1. False negative. PS00503. PECTINESTERASE_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PME66_ARATH

Accession

Primary (citable) accession number: Q4PSQ5
Secondary accession number(s): O22902

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	May 5, 2009
Last modified:	December 14, 2011
This is version 41 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program