Reviewed, UniProtKB/Swiss-Prot Q4PSN0 (PME29_ARATH)

Names and origin

Protein names

Recommended name:
    Probable pectinesterase 29
      Short name=PE 29
    EC=3.1.1.11
Alternative name(s):
    Pectin methylesterase 29
      Short name=AtPME29

Gene names

Name:	PME29
Synonyms:	ARATH29
Ordered Locus Names:	At3g24130
ORF Names:	MUJ8.16

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	335 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Acts in the modification of cell walls via demethylesterification of cell wall pectin By similarity.
Catalytic activity	Pectin + n H2O = n methanol + pectate.
Pathway	Glycan metabolism; pectin degradation; 2-dehydro-3-deoxy-D-gluconate from pectin: step 1/5.
Subcellular location	Secreted › cell wall By similarity.
Tissue specificity	Expressed in flower buds. Ref.4
Sequence similarities	Belongs to the pectinesterase family.
Sequence caution	The sequence BAB01354.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell wall Secreted
Domain	Signal
Molecular function	Aspartyl esterase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell wall modification Inferred from electronic annotation. Source: InterPro
Cellular component	cell wall Inferred from electronic annotation. Source: UniProtKB-SubCell extracellular region Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	aspartyl esterase activity Inferred from electronic annotation. Source: UniProtKB-KW pectinesterase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 335	311	Probable pectinesterase 29		PRO_0000371682
Sites
	Active site	166	1	Proton donor By similarity
	Active site	187	1	Nucleophile By similarity
	Binding site	248	1	Substrate By similarity
	Binding site	250	1	Substrate By similarity
	Site	165	1	Transition state stabilizer By similarity
Amino acid modifications
	Glycosylation	43	1	N-linked (GlcNAc...) Potential
	Glycosylation	262	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q4PSN0-1 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: 1C3CB972B2E66EDB	FASTA	335	37,272
	10 20 30 40 50 60 MGTHRIFIGL IALCCFCLPH LIEAKPFGVY QQQVFVDQSG HGNFTTIQKA IDSVPINNRH 70 80 90 100 110 120 WFFINVKAGL YREKIKIPYE KPFIVLVGAG KRLTRVEWDD HYSVAQSPTF STLADNTVVK 130 140 150 160 170 180 SITFANSYNF PSKGKMNKNP RTPAVAALIG GDKSAFYSVG FAGIQDTLWD FDGRHYFHRC 190 200 210 220 230 240 TIQGAVDFIF GTGQSIYQSC VIQVLGGQLE PGLAGYITAQ GRTNPYDANG FIFINCLVYG 250 260 270 280 290 300 TGMAFLGRPW RGYSRVIFYN SNLTDVVVPE GWDAWNFVGH ENQLVFAEHG CFGSGANIGR 310 320 330 RVKWVKKLSE SAIQNLADLS FINRGGWVED LPIPA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones." Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S. DNA Res. 7:131-135(2000) [PubMed: 10819329] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	Underwood B.A., Xiao Y.-L., Moskal W.A. Jr., Monaghan E.L., Wang W., Redman J.C., Wu H.C., Utterback T., Town C.D. Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[3]	"Pectin methylesterases: sequence-structural features and phylogenetic relationships." Markovic O., Janecek S. Carbohydr. Res. 339:2281-2295(2004) [PubMed: 15337457] [Abstract] Cited for: GENE FAMILY, NOMENCLATURE.
[4]	"Comprehensive expression profiling of the pectin methylesterase gene family during silique development in Arabidopsis thaliana." Louvet R., Cavel E., Gutierrez L., Guenin S., Roger D., Gillet F., Guerineau F., Pelloux J. Planta 224:782-791(2006) [PubMed: 16622707] [Abstract] Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB028621 Genomic DNA. Translation: BAB01354.1. Sequence problems. DQ056606 mRNA. Translation: AAY78754.1.
IPI	IPI00527818.
RefSeq	NP_189055.1.
UniGene	At.53468
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	821999.
GenomeReviews	Gene locus AT3G24130 in contig BA000014_GR.
KEGG	ath:AT3G24130.
NMPDR	fig|3702.1.peg.14673.
Organism-specific databases
GeneFarm	456. 8.
TAIR	At3g24130.
Phylogenomic databases
OMA	WVTAHAR.
Gene expression databases
Genevestigator	Q4PSN0.
Family and domain databases
InterPro	IPR012334. Pectin_lyas_fold. IPR018040. Pectinesterase_AS. IPR000070. Pectinesterase_cat. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF01095. Pectinesterase. 1 hit. [Graphical view]
PROSITE	PS00800. PECTINESTERASE_1. False negative. PS00503. PECTINESTERASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PME29_ARATH

Accession

Primary (citable) accession number: Q4PSN0
Secondary accession number(s): Q9LRN4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	July 19, 2005
Last modified:	November 3, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)