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Protein

Glutamate decarboxylase 2

Gene

GAD2

Organism
Canis familiaris (Dog) (Canis lupus familiaris)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the production of GABA.

Catalytic activityi

L-glutamate = 4-aminobutanoate + CO2.

Cofactori

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei558 – 5581SubstrateBy similarity

GO - Molecular functioni

  1. glutamate decarboxylase activity Source: UniProtKB-EC
  2. pyridoxal phosphate binding Source: InterPro

GO - Biological processi

  1. carboxylic acid metabolic process Source: InterPro
  2. neurotransmitter biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Neurotransmitter biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate decarboxylase 2 (EC:4.1.1.15)
Alternative name(s):
65 kDa glutamic acid decarboxylase
Short name:
GAD-65
Glutamate decarboxylase 65 kDa isoform
Gene namesi
Name:GAD2
Synonyms:GAD65
OrganismiCanis familiaris (Dog) (Canis lupus familiaris)
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
ProteomesiUP000002254: Unplaced

Subcellular locationi

Cytoplasmcytosol By similarity. Cytoplasmic vesicle By similarity. Cell junctionsynapsepresynaptic cell membrane By similarity; Lipid-anchor By similarity. Golgi apparatus membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
Note: Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters (By similarity).By similarity

GO - Cellular componenti

  1. cell junction Source: UniProtKB-KW
  2. cytoplasmic membrane-bounded vesicle Source: UniProtKB-SubCell
  3. cytosol Source: UniProtKB-SubCell
  4. Golgi membrane Source: UniProtKB-SubCell
  5. presynaptic membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 585585Glutamate decarboxylase 2PRO_0000231042Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei3 – 31PhosphoserineBy similarity
Modified residuei6 – 61PhosphoserineBy similarity
Modified residuei10 – 101PhosphoserineBy similarity
Modified residuei13 – 131PhosphoserineBy similarity
Lipidationi30 – 301S-palmitoyl cysteineBy similarity
Lipidationi45 – 451S-palmitoyl cysteineBy similarity
Modified residuei396 – 3961N6-(pyridoxal phosphate)lysineBy similarity

Post-translational modificationi

Phosphorylated; which does not affect kinetic parameters or subcellular location.By similarity
Palmitoylated; which is required for presynaptic clustering.By similarity

Keywords - PTMi

Lipoprotein, Palmitate, Phosphoprotein

Interactioni

Subunit structurei

Homodimer.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ4PRC2.
SMRiQ4PRC2. Positions 88-584.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni181 – 1833Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the group II decarboxylase family.Curated

Phylogenomic databases

eggNOGiCOG0076.
HOGENOMiHOG000005382.
HOVERGENiHBG004980.
InParanoidiQ4PRC2.
KOiK01580.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR002129. PyrdxlP-dep_de-COase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR021115. Pyridoxal-P_BS.
[Graphical view]
PfamiPF00282. Pyridoxal_deC. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00392. DDC_GAD_HDC_YDC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4PRC2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASPGSGFWS FGSEDGSGDP ENPSTARAWC QVAQKFTGGI GNKLCALLYG
60 70 80 90 100
DAEKPAESGG SEPPRATSRK AACACNQKPC SCPKAEVNYA FLHATDLLPA
110 120 130 140 150
CDGERPTLAF LQDVMDILLQ YVVKSFDRST KVIDFHYPNE LLQEYNWELA
160 170 180 190 200
DQPQNLEEIL MHCQTTLKYA IKTGHPRYFN QLSTGLDMVG LAADWLTSTA
210 220 230 240 250
NTNMFTYEIA PVFVLLEYVT LKKMREIIGW PGGSGDGIFS PGGAISNMYA
260 270 280 290 300
MLIARFKMFP EVKEKGMAAV PRLIAFTSEH SHFSLKKGAA ALGIGTDSVI
310 320 330 340 350
LIKCDERGKM VPSDLERRIL EAKQKGFVPF LVSATAGTTV YGAFDPLLAV
360 370 380 390 400
ADICKKYKIW MHVDAAWGGG LLMSRKHKWK LSGVERANSV TWNPHKMMGV
410 420 430 440 450
PLQCSALLVR EEGLMQSCNQ MHASYLFQQD KHYDLSYDTG DKALQCGRHV
460 470 480 490 500
DVFKLWLMWR AKGTTGFEAH IDKCLELAEY LYSIIKNREG YEMVFDGKPQ
510 520 530 540 550
HTNVCFWYVP PSLRVLEDNE ERMNRLSKVA PVIKARMMEY GTTMVSYQPL
560 570 580
GDKVNFFRMV ISNPAATHQD IDFLIEEIER LGQDL
Length:585
Mass (Da):65,419
Last modified:July 19, 2005 - v1
Checksum:i55BDA189F62922B2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ060442 mRNA. Translation: AAY59421.1.
AB261623 mRNA. Translation: BAF37948.1.
RefSeqiNP_001070907.1. NM_001077439.1.
UniGeneiCfa.24099.

Genome annotation databases

GeneIDi487107.
KEGGicfa:487107.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ060442 mRNA. Translation: AAY59421.1.
AB261623 mRNA. Translation: BAF37948.1.
RefSeqiNP_001070907.1. NM_001077439.1.
UniGeneiCfa.24099.

3D structure databases

ProteinModelPortaliQ4PRC2.
SMRiQ4PRC2. Positions 88-584.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi487107.
KEGGicfa:487107.

Organism-specific databases

CTDi2572.

Phylogenomic databases

eggNOGiCOG0076.
HOGENOMiHOG000005382.
HOVERGENiHBG004980.
InParanoidiQ4PRC2.
KOiK01580.

Miscellaneous databases

NextBioi20860754.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR002129. PyrdxlP-dep_de-COase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR021115. Pyridoxal-P_BS.
[Graphical view]
PfamiPF00282. Pyridoxal_deC. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00392. DDC_GAD_HDC_YDC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Autoantibodies to recombinant canine GAD65 in canine diabetes mellitus."
    Davison L.J., Weenink S.M., Christie M.R., Herrtage M.E., Catchpole B.
    Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "Sequences of canine glutamate decarboxylase (GAD) 1 and GAD2 genes, and variation of their genetic polymorphisms among five dog breeds."
    Arata S., Hashizume C., Kikusui T., Takeuchi Y., Mori Y.
    J. Vet. Med. Sci. 70:1107-1110(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiDCE2_CANFA
AccessioniPrimary (citable) accession number: Q4PRC2
Secondary accession number(s): A0PA84
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: July 19, 2005
Last modified: January 7, 2015
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.