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Q4PRC2

- DCE2_CANFA

UniProt

Q4PRC2 - DCE2_CANFA

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Protein
Glutamate decarboxylase 2
Gene
GAD2, GAD65
Organism
Canis familiaris (Dog) (Canis lupus familiaris)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes the production of GABA.

Catalytic activityi

L-glutamate = 4-aminobutanoate + CO2.

Cofactori

Pyridoxal phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei558 – 5581Substrate By similarity

GO - Molecular functioni

  1. glutamate decarboxylase activity Source: UniProtKB-EC
  2. pyridoxal phosphate binding Source: InterPro

GO - Biological processi

  1. carboxylic acid metabolic process Source: InterPro
  2. neurotransmitter biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Neurotransmitter biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate decarboxylase 2 (EC:4.1.1.15)
Alternative name(s):
65 kDa glutamic acid decarboxylase
Short name:
GAD-65
Glutamate decarboxylase 65 kDa isoform
Gene namesi
Name:GAD2
Synonyms:GAD65
OrganismiCanis familiaris (Dog) (Canis lupus familiaris)
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
ProteomesiUP000002254: Unplaced

Subcellular locationi

Cytoplasmcytosol By similarity. Cytoplasmic vesicle By similarity. Cell junctionsynapsepresynaptic cell membrane; Lipid-anchor By similarity. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side By similarity
Note: Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters By similarity.

GO - Cellular componenti

  1. Golgi membrane Source: UniProtKB-SubCell
  2. cell junction Source: UniProtKB-KW
  3. cytoplasmic membrane-bounded vesicle Source: UniProtKB-SubCell
  4. cytosol Source: UniProtKB-SubCell
  5. presynaptic membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 585585Glutamate decarboxylase 2
PRO_0000231042Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei3 – 31Phosphoserine By similarity
Modified residuei6 – 61Phosphoserine By similarity
Modified residuei10 – 101Phosphoserine By similarity
Modified residuei13 – 131Phosphoserine By similarity
Lipidationi30 – 301S-palmitoyl cysteine By similarity
Lipidationi45 – 451S-palmitoyl cysteine By similarity
Modified residuei396 – 3961N6-(pyridoxal phosphate)lysine By similarity

Post-translational modificationi

Phosphorylated; which does not affect kinetic parameters or subcellular location By similarity.
Palmitoylated; which is required for presynaptic clustering By similarity.

Keywords - PTMi

Lipoprotein, Palmitate, Phosphoprotein

Interactioni

Subunit structurei

Homodimer By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ4PRC2.
SMRiQ4PRC2. Positions 88-584.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni181 – 1833Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0076.
HOGENOMiHOG000005382.
HOVERGENiHBG004980.
InParanoidiQ4PRC2.
KOiK01580.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR002129. PyrdxlP-dep_de-COase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR021115. Pyridoxal-P_BS.
[Graphical view]
PfamiPF00282. Pyridoxal_deC. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00392. DDC_GAD_HDC_YDC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4PRC2-1 [UniParc]FASTAAdd to Basket

« Hide

MASPGSGFWS FGSEDGSGDP ENPSTARAWC QVAQKFTGGI GNKLCALLYG    50
DAEKPAESGG SEPPRATSRK AACACNQKPC SCPKAEVNYA FLHATDLLPA 100
CDGERPTLAF LQDVMDILLQ YVVKSFDRST KVIDFHYPNE LLQEYNWELA 150
DQPQNLEEIL MHCQTTLKYA IKTGHPRYFN QLSTGLDMVG LAADWLTSTA 200
NTNMFTYEIA PVFVLLEYVT LKKMREIIGW PGGSGDGIFS PGGAISNMYA 250
MLIARFKMFP EVKEKGMAAV PRLIAFTSEH SHFSLKKGAA ALGIGTDSVI 300
LIKCDERGKM VPSDLERRIL EAKQKGFVPF LVSATAGTTV YGAFDPLLAV 350
ADICKKYKIW MHVDAAWGGG LLMSRKHKWK LSGVERANSV TWNPHKMMGV 400
PLQCSALLVR EEGLMQSCNQ MHASYLFQQD KHYDLSYDTG DKALQCGRHV 450
DVFKLWLMWR AKGTTGFEAH IDKCLELAEY LYSIIKNREG YEMVFDGKPQ 500
HTNVCFWYVP PSLRVLEDNE ERMNRLSKVA PVIKARMMEY GTTMVSYQPL 550
GDKVNFFRMV ISNPAATHQD IDFLIEEIER LGQDL 585
Length:585
Mass (Da):65,419
Last modified:July 19, 2005 - v1
Checksum:i55BDA189F62922B2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ060442 mRNA. Translation: AAY59421.1.
AB261623 mRNA. Translation: BAF37948.1.
RefSeqiNP_001070907.1. NM_001077439.1.
UniGeneiCfa.24099.

Genome annotation databases

GeneIDi487107.
KEGGicfa:487107.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
DQ060442 mRNA. Translation: AAY59421.1 .
AB261623 mRNA. Translation: BAF37948.1 .
RefSeqi NP_001070907.1. NM_001077439.1.
UniGenei Cfa.24099.

3D structure databases

ProteinModelPortali Q4PRC2.
SMRi Q4PRC2. Positions 88-584.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 487107.
KEGGi cfa:487107.

Organism-specific databases

CTDi 2572.

Phylogenomic databases

eggNOGi COG0076.
HOGENOMi HOG000005382.
HOVERGENi HBG004980.
InParanoidi Q4PRC2.
KOi K01580.

Miscellaneous databases

NextBioi 20860754.

Family and domain databases

Gene3Di 3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProi IPR002129. PyrdxlP-dep_de-COase.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
IPR021115. Pyridoxal-P_BS.
[Graphical view ]
Pfami PF00282. Pyridoxal_deC. 1 hit.
[Graphical view ]
SUPFAMi SSF53383. SSF53383. 1 hit.
PROSITEi PS00392. DDC_GAD_HDC_YDC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Autoantibodies to recombinant canine GAD65 in canine diabetes mellitus."
    Davison L.J., Weenink S.M., Christie M.R., Herrtage M.E., Catchpole B.
    Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "Sequences of canine glutamate decarboxylase (GAD) 1 and GAD2 genes, and variation of their genetic polymorphisms among five dog breeds."
    Arata S., Hashizume C., Kikusui T., Takeuchi Y., Mori Y.
    J. Vet. Med. Sci. 70:1107-1110(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiDCE2_CANFA
AccessioniPrimary (citable) accession number: Q4PRC2
Secondary accession number(s): A0PA84
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 4, 2006
Last sequence update: July 19, 2005
Last modified: December 11, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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