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Q4PRC2

- DCE2_CANFA

UniProt

Q4PRC2 - DCE2_CANFA

Protein

Glutamate decarboxylase 2

Gene

GAD2

Organism
Canis familiaris (Dog) (Canis lupus familiaris)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 68 (01 Oct 2014)
      Sequence version 1 (19 Jul 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the production of GABA.

    Catalytic activityi

    L-glutamate = 4-aminobutanoate + CO2.

    Cofactori

    Pyridoxal phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei558 – 5581SubstrateBy similarity

    GO - Molecular functioni

    1. glutamate decarboxylase activity Source: UniProtKB-EC
    2. pyridoxal phosphate binding Source: InterPro

    GO - Biological processi

    1. carboxylic acid metabolic process Source: InterPro
    2. neurotransmitter biosynthetic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Decarboxylase, Lyase

    Keywords - Biological processi

    Neurotransmitter biosynthesis

    Keywords - Ligandi

    Pyridoxal phosphate

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate decarboxylase 2 (EC:4.1.1.15)
    Alternative name(s):
    65 kDa glutamic acid decarboxylase
    Short name:
    GAD-65
    Glutamate decarboxylase 65 kDa isoform
    Gene namesi
    Name:GAD2
    Synonyms:GAD65
    OrganismiCanis familiaris (Dog) (Canis lupus familiaris)
    Taxonomic identifieri9615 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
    ProteomesiUP000002254: Unplaced

    Subcellular locationi

    Cytoplasmcytosol By similarity. Cytoplasmic vesicle By similarity. Cell junctionsynapsepresynaptic cell membrane By similarity; Lipid-anchor By similarity. Golgi apparatus membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
    Note: Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters By similarity.By similarity

    GO - Cellular componenti

    1. cell junction Source: UniProtKB-KW
    2. cytoplasmic membrane-bounded vesicle Source: UniProtKB-SubCell
    3. cytosol Source: UniProtKB-SubCell
    4. Golgi membrane Source: UniProtKB-SubCell
    5. presynaptic membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Membrane, Synapse

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 585585Glutamate decarboxylase 2PRO_0000231042Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei3 – 31PhosphoserineBy similarity
    Modified residuei6 – 61PhosphoserineBy similarity
    Modified residuei10 – 101PhosphoserineBy similarity
    Modified residuei13 – 131PhosphoserineBy similarity
    Lipidationi30 – 301S-palmitoyl cysteineBy similarity
    Lipidationi45 – 451S-palmitoyl cysteineBy similarity
    Modified residuei396 – 3961N6-(pyridoxal phosphate)lysineBy similarity

    Post-translational modificationi

    Phosphorylated; which does not affect kinetic parameters or subcellular location.By similarity
    Palmitoylated; which is required for presynaptic clustering.By similarity

    Keywords - PTMi

    Lipoprotein, Palmitate, Phosphoprotein

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ4PRC2.
    SMRiQ4PRC2. Positions 88-584.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni181 – 1833Substrate bindingBy similarity

    Sequence similaritiesi

    Belongs to the group II decarboxylase family.Curated

    Phylogenomic databases

    eggNOGiCOG0076.
    HOGENOMiHOG000005382.
    HOVERGENiHBG004980.
    InParanoidiQ4PRC2.
    KOiK01580.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProiIPR002129. PyrdxlP-dep_de-COase.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    IPR021115. Pyridoxal-P_BS.
    [Graphical view]
    PfamiPF00282. Pyridoxal_deC. 1 hit.
    [Graphical view]
    SUPFAMiSSF53383. SSF53383. 1 hit.
    PROSITEiPS00392. DDC_GAD_HDC_YDC. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q4PRC2-1 [UniParc]FASTAAdd to Basket

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    MASPGSGFWS FGSEDGSGDP ENPSTARAWC QVAQKFTGGI GNKLCALLYG    50
    DAEKPAESGG SEPPRATSRK AACACNQKPC SCPKAEVNYA FLHATDLLPA 100
    CDGERPTLAF LQDVMDILLQ YVVKSFDRST KVIDFHYPNE LLQEYNWELA 150
    DQPQNLEEIL MHCQTTLKYA IKTGHPRYFN QLSTGLDMVG LAADWLTSTA 200
    NTNMFTYEIA PVFVLLEYVT LKKMREIIGW PGGSGDGIFS PGGAISNMYA 250
    MLIARFKMFP EVKEKGMAAV PRLIAFTSEH SHFSLKKGAA ALGIGTDSVI 300
    LIKCDERGKM VPSDLERRIL EAKQKGFVPF LVSATAGTTV YGAFDPLLAV 350
    ADICKKYKIW MHVDAAWGGG LLMSRKHKWK LSGVERANSV TWNPHKMMGV 400
    PLQCSALLVR EEGLMQSCNQ MHASYLFQQD KHYDLSYDTG DKALQCGRHV 450
    DVFKLWLMWR AKGTTGFEAH IDKCLELAEY LYSIIKNREG YEMVFDGKPQ 500
    HTNVCFWYVP PSLRVLEDNE ERMNRLSKVA PVIKARMMEY GTTMVSYQPL 550
    GDKVNFFRMV ISNPAATHQD IDFLIEEIER LGQDL 585
    Length:585
    Mass (Da):65,419
    Last modified:July 19, 2005 - v1
    Checksum:i55BDA189F62922B2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ060442 mRNA. Translation: AAY59421.1.
    AB261623 mRNA. Translation: BAF37948.1.
    RefSeqiNP_001070907.1. NM_001077439.1.
    UniGeneiCfa.24099.

    Genome annotation databases

    GeneIDi487107.
    KEGGicfa:487107.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    DQ060442 mRNA. Translation: AAY59421.1 .
    AB261623 mRNA. Translation: BAF37948.1 .
    RefSeqi NP_001070907.1. NM_001077439.1.
    UniGenei Cfa.24099.

    3D structure databases

    ProteinModelPortali Q4PRC2.
    SMRi Q4PRC2. Positions 88-584.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 487107.
    KEGGi cfa:487107.

    Organism-specific databases

    CTDi 2572.

    Phylogenomic databases

    eggNOGi COG0076.
    HOGENOMi HOG000005382.
    HOVERGENi HBG004980.
    InParanoidi Q4PRC2.
    KOi K01580.

    Miscellaneous databases

    NextBioi 20860754.

    Family and domain databases

    Gene3Di 3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProi IPR002129. PyrdxlP-dep_de-COase.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    IPR021115. Pyridoxal-P_BS.
    [Graphical view ]
    Pfami PF00282. Pyridoxal_deC. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53383. SSF53383. 1 hit.
    PROSITEi PS00392. DDC_GAD_HDC_YDC. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Autoantibodies to recombinant canine GAD65 in canine diabetes mellitus."
      Davison L.J., Weenink S.M., Christie M.R., Herrtage M.E., Catchpole B.
      Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    2. "Sequences of canine glutamate decarboxylase (GAD) 1 and GAD2 genes, and variation of their genetic polymorphisms among five dog breeds."
      Arata S., Hashizume C., Kikusui T., Takeuchi Y., Mori Y.
      J. Vet. Med. Sci. 70:1107-1110(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiDCE2_CANFA
    AccessioniPrimary (citable) accession number: Q4PRC2
    Secondary accession number(s): A0PA84
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 4, 2006
    Last sequence update: July 19, 2005
    Last modified: October 1, 2014
    This is version 68 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3