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Protein

Lanosterol 14-alpha demethylase

Gene

CYP51A1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes C14-demethylation of lanosterol; it transforms lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol.

Catalytic activityi

A 14-alpha-methylsteroid + 3 O2 + 3 NADPH = a Delta(14)-steroid + formate + 3 NADP+ + 4 H2O.

Cofactori

hemeBy similarity

Pathwayi: zymosterol biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes zymosterol from lanosterol.
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Lanosterol 14-alpha demethylase (CYP51A1)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating (NSDHL)
  5. no protein annotated in this organism
  6. no protein annotated in this organism
This subpathway is part of the pathway zymosterol biosynthesis, which is itself part of Steroid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes zymosterol from lanosterol, the pathway zymosterol biosynthesis and in Steroid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi448Iron (heme axial ligand)By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Cholesterol biosynthesis, Cholesterol metabolism, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

BRENDAi1.14.13.70. 908.
ReactomeiR-BTA-191273. Cholesterol biosynthesis.
R-BTA-211976. Endogenous sterols.
R-BTA-2426168. Activation of gene expression by SREBF (SREBP).
UniPathwayiUPA00770; UER00754.

Names & Taxonomyi

Protein namesi
Recommended name:
Lanosterol 14-alpha demethylase (EC:1.14.13.70)
Short name:
LDM
Alternative name(s):
CYPLI
Cytochrome P450 51A1
Cytochrome P450-14DM
Short name:
Cytochrome P45014DM
Cytochrome P450LI
Sterol 14-alpha demethylase
Gene namesi
Name:CYP51A1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 4

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei22 – 42HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000519971 – 502Lanosterol 14-alpha demethylaseAdd BLAST502

Proteomic databases

PaxDbiQ4PJW3.
PRIDEiQ4PJW3.

Expressioni

Gene expression databases

BgeeiENSBTAG00000001992.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000002582.

Structurei

3D structure databases

ProteinModelPortaliQ4PJW3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0684. Eukaryota.
COG2124. LUCA.
GeneTreeiENSGT00800000124060.
HOGENOMiHOG000042780.
HOVERGENiHBG051102.
InParanoidiQ4PJW3.
KOiK05917.
OMAiMIHTPHN.
OrthoDBiEOG091G04XH.
TreeFamiTF105091.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00465. EP450IV.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4PJW3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLDLLQAGGS VLGQAMEQVT GGNLASMLLI ACAFTLSLVY LFRLAVGHLA
60 70 80 90 100
PPLPTGAKSP PYIVSPIPFL GHAIAFGKSP IEFLEDAYEK YGPVFSFTMV
110 120 130 140 150
GKTFTYLLGS EAAALLFNSK NEDLNAEEVY SRLTTPVFGK GVAYDVPNTV
160 170 180 190 200
FLEQKKMLKS GLNIAHFRQH VSIIEKETKE YFKSWGESGE KNLFEALSEL
210 220 230 240 250
IILTASHCLH GKEIRSQLNE KVAQLYADLD GGFSHAAWLL PGWLPLPSFR
260 270 280 290 300
RRDRAHREIK NIFYKAIQKR RESGEKIDDI LQTLLESTYK DGRPLTDDEV
310 320 330 340 350
AGMLIGLLLA GQHTSSTTSA WMGFFLARDK TLQEKCFLEQ KTVCGENLPP
360 370 380 390 400
LTYDQLKDLN LLDRCIKETL RLRPPIMTMM RLAKTPLTVA GYTIPPGHQV
410 420 430 440 450
CVSPTVNQRL KDSWVERLDF NPDRYLEDSP ASGEKFAYVP FGAGRHRCIG
460 470 480 490 500
ENFAYVQIKT IWSTMLRLYE FDLIDGYFPT VNYTTMIHTP EKPIIRYKRR

SK
Length:502
Mass (Da):56,596
Last modified:July 19, 2005 - v1
Checksum:i5D1C5AFA43E067A1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ078267 mRNA. Translation: AAY82452.1.
DQ085625 mRNA. Translation: AAZ74630.1.
BC149346 mRNA. Translation: AAI49347.1.
RefSeqiNP_001020490.1. NM_001025319.2.
UniGeneiBt.49631.

Genome annotation databases

EnsembliENSBTAT00000002582; ENSBTAP00000002582; ENSBTAG00000001992.
GeneIDi505060.
KEGGibta:505060.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
DQ078267 mRNA. Translation: AAY82452.1.
DQ085625 mRNA. Translation: AAZ74630.1.
BC149346 mRNA. Translation: AAI49347.1.
RefSeqiNP_001020490.1. NM_001025319.2.
UniGeneiBt.49631.

3D structure databases

ProteinModelPortaliQ4PJW3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000002582.

Proteomic databases

PaxDbiQ4PJW3.
PRIDEiQ4PJW3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000002582; ENSBTAP00000002582; ENSBTAG00000001992.
GeneIDi505060.
KEGGibta:505060.

Organism-specific databases

CTDi1595.

Phylogenomic databases

eggNOGiKOG0684. Eukaryota.
COG2124. LUCA.
GeneTreeiENSGT00800000124060.
HOGENOMiHOG000042780.
HOVERGENiHBG051102.
InParanoidiQ4PJW3.
KOiK05917.
OMAiMIHTPHN.
OrthoDBiEOG091G04XH.
TreeFamiTF105091.

Enzyme and pathway databases

UniPathwayiUPA00770; UER00754.
BRENDAi1.14.13.70. 908.
ReactomeiR-BTA-191273. Cholesterol biosynthesis.
R-BTA-211976. Endogenous sterols.
R-BTA-2426168. Activation of gene expression by SREBF (SREBP).

Gene expression databases

BgeeiENSBTAG00000001992.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002403. Cyt_P450_E_grp-IV.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00465. EP450IV.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCP51A_BOVIN
AccessioniPrimary (citable) accession number: Q4PJW3
Secondary accession number(s): A6QPJ2, Q3YM99
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: July 19, 2005
Last modified: October 5, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.