Reviewed, UniProtKB/Swiss-Prot Q4PJW3 (CP51A_BOVIN)

Names and origin

Protein names

Recommended name:
    Lanosterol 14-alpha demethylase
      Short name=LDM
    EC=1.14.13.70
Alternative name(s):
    Cytochrome P450 51A1
    CYPLI
    P450LI
    Sterol 14-alpha demethylase
    P450-14DM
      Short name=P45014DM

Gene names

Name:

CYP51A1

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	502 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes C14-demethylation of lanosterol; it transforms lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol.
Catalytic activity	Obtusifoliol + 3 O2 + 3 NADPH = 4-alpha-methyl-5-alpha-ergosta-8,14,24(28)-trien-3-beta-ol + formate + 3 NADP+ + 4 H2O.
Cofactor	Heme group By similarity.
Pathway	Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 1/6.
Subcellular location	Endoplasmic reticulum membrane By similarity. Microsome membrane By similarity.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Biological process	Cholesterol biosynthesis Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Endoplasmic reticulum Membrane Microsome
Domain	Transmembrane
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	cholesterol biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro sterol 14-demethylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 502	502	Lanosterol 14-alpha demethylase		PRO_0000051997
Regions
	Transmembrane	22 – 42	21	Potential
Sites
	Metal binding	448	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4PJW3-1 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: 5D1C5AFA43E067A1	FASTA	502	56,596
	10 20 30 40 50 60 MLDLLQAGGS VLGQAMEQVT GGNLASMLLI ACAFTLSLVY LFRLAVGHLA PPLPTGAKSP 70 80 90 100 110 120 PYIVSPIPFL GHAIAFGKSP IEFLEDAYEK YGPVFSFTMV GKTFTYLLGS EAAALLFNSK 130 140 150 160 170 180 NEDLNAEEVY SRLTTPVFGK GVAYDVPNTV FLEQKKMLKS GLNIAHFRQH VSIIEKETKE 190 200 210 220 230 240 YFKSWGESGE KNLFEALSEL IILTASHCLH GKEIRSQLNE KVAQLYADLD GGFSHAAWLL 250 260 270 280 290 300 PGWLPLPSFR RRDRAHREIK NIFYKAIQKR RESGEKIDDI LQTLLESTYK DGRPLTDDEV 310 320 330 340 350 360 AGMLIGLLLA GQHTSSTTSA WMGFFLARDK TLQEKCFLEQ KTVCGENLPP LTYDQLKDLN 370 380 390 400 410 420 LLDRCIKETL RLRPPIMTMM RLAKTPLTVA GYTIPPGHQV CVSPTVNQRL KDSWVERLDF 430 440 450 460 470 480 NPDRYLEDSP ASGEKFAYVP FGAGRHRCIG ENFAYVQIKT IWSTMLRLYE FDLIDGYFPT 490 500 VNYTTMIHTP EKPIIRYKRR SK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Pre-cholesterol precursors in gametogenesis." Rozman D., Seliskar M., Cotman M., Fink M. Mol. Cell. Endocrinol. 234:47-56(2005) [PubMed: 15836952] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Testis.
[2]	"cDNA cloning, genomic structure and expression analysis of the bovine lanosterol 14alpha-demethylase (CYP51) in gonads." Wang F., Shen Y., Song X., Xia G., Chen X., Zhou B., Lei L. Biol. Pharm. Bull. 29:430-436(2006) [PubMed: 16508140] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	NIH - Mammalian Gene Collection (MGC) project Submitted (JUL-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Fetal medulla.

Cross-references

Sequence databases
EMBLGenBankDDBJ	DQ078267 mRNA. Translation: AAY82452.1. DQ085625 mRNA. Translation: AAZ74630.1. BC149346 mRNA. Translation: AAI49347.1.
IPI	IPI00705027.
RefSeq	NP_001020490.1.
UniGene	Bt.49631
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000001992. Bos taurus. [Contig view]
GeneID	505060.
KEGG	bta:505060.
Phylogenomic databases
HOVERGEN	Q4PJW3.
Enzyme and pathway databases
BRENDA	1.14.13.70. 251.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002403. Cyt_P450_E_grp-IV. [Graphical view]
Gene3D	G3DSA:1.10.630.10. Cyt_P450. 1 hit.
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00465. EP450IV. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CP51A_BOVIN

Accession

Primary (citable) accession number: Q4PJW3
Secondary accession number(s): A6QPJ2, Q3YM99

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	July 19, 2005
Last modified:	June 16, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)