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Q4PJW2 (NCOA1_PIG) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Nuclear receptor coactivator 1
Short name=NCoA-1
EC=2.3.1.48
Alternative name(s):
Steroid receptor coactivator 1
Short name=SRC-1
Gene names
Name:NCOA1
Synonyms:SRC1
OrganismSus scrofa (Pig) [Reference proteome]
Taxonomic identifier9823 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length1440 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Involved in the coactivation of different nuclear receptors, such as for steroids (PGR, GR and ER), retinoids (RXRs), thyroid hormone (TRs) and prostanoids (PPARs). Also involved in coactivation mediated by STAT3, STAT5A, STAT5B and STAT6 transcription factors. Displays histone acetyltransferase activity toward H3 and H4; the relevance of such activity remains however unclear. Plays a central role in creating multisubunit coactivator complexes that act via remodeling of chromatin, and possibly acts by participating in both chromatin remodeling and recruitment of general transcription factors. Required with NCOA2 to control energy balance between white and brown adipose tissues. Required for mediating steroid hormone response By similarity.

Catalytic activity

Acetyl-CoA + [histone] = CoA + acetyl-[histone].

Subunit structure

Interacts with NCOA6 and NCOA2. Interacts with the FDL motif of STAT5A and STAT5B. Interacts with the LXXLL motif of STAT6. Interacts with STAT3 following IL-6 stimulation. Interacts with the basal transcription factor GTF2B. Interacts with COPS5, NR3C1, PCAF and TTLL5/STAMP. Interacts with the histone acetyltransferases EP300 and CREBBP, and the methyltransferase CARM1. Interacts with UBE2L3; they functionally interact to regulate progesterone receptor transcriptional activity. Interacts with PRMT2 and DDX5. Interacts with ASXL1 By similarity.

Subcellular location

Nucleus By similarity.

Domain

The C-terminal (1107-1447) part mediates the histone acetyltransferase (HAT) activity By similarity.

Contains 7 Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs. LXXLL motifs 3, 4 and 5 are essential for the association with nuclear receptors.

Post-translational modification

Sumoylated; sumoylation increases its interaction with PGR and prolongs its retention in the nucleus. It does not prevent its ubiquitination and does not exert a clear effect on the stability of the protein By similarity.

Ubiquitinated; leading to proteasome-mediated degradation. Ubiquitination and sumoylation take place at different sites By similarity.

Sequence similarities

Belongs to the SRC/p160 nuclear receptor coactivator family.

Contains 1 bHLH (basic helix-loop-helix) domain.

Contains 1 PAS (PER-ARNT-SIM) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 14401440Nuclear receptor coactivator 1
PRO_0000300687

Regions

Domain23 – 8058bHLH
Domain109 – 18072PAS
Region361 – 567207Interaction with STAT3 By similarity
Region781 – 988208Interaction with CREBBP By similarity
Motif46 – 505LXXLL motif 1 By similarity
Motif112 – 1165LXXLL motif 2 By similarity
Motif633 – 6375LXXLL motif 3 By similarity
Motif690 – 6945LXXLL motif 4 By similarity
Motif749 – 7535LXXLL motif 5 By similarity
Motif913 – 9175LXXLL motif 6 By similarity
Motif1434 – 14385LXXLL motif 7 By similarity
Compositional bias389 – 682294Ser-rich
Compositional bias1053 – 113886Gln-rich

Amino acid modifications

Modified residue221Phosphoserine By similarity
Modified residue3721Phosphoserine By similarity
Modified residue3951Phosphoserine By similarity
Modified residue5171Phosphoserine By similarity
Modified residue5691Phosphoserine By similarity
Modified residue10331Phosphoserine By similarity
Modified residue11791Phosphothreonine By similarity
Modified residue11851Phosphoserine By similarity
Cross-link732Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity
Cross-link774Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4PJW2 [UniParc].

Last modified July 19, 2005. Version 1.
Checksum: 79B850ED7958AF50

FASTA1,440156,925
        10         20         30         40         50         60 
MSGLGDSSSD PANPDSHKRK GSPCDTLASS TEKRRREQEN KYLEELAELL SANISDIDSL 

        70         80         90        100        110        120 
SVKPDKCKIL KKTVDQIQLM KRMEQEKSTT DDEVQKSDIS SSSQGVIEKE SLGPLLLEAL 

       130        140        150        160        170        180 
DGFFFVVNCE GRIVFVSENV TSYLGYNQEE LMNTSVYSIL HVGDHAEFVK NLLPKSLVNG 

       190        200        210        220        230        240 
VPWPQEATRR NSHTFNCRML IHPPDEPGTE NQEACQRYEV MQCFTVSQPK SIQEDGEDFQ 

       250        260        270        280        290        300 
SCLICIARRL PRPPAIMGVE SFMTKQDTTG KIISIDTSSL RAAGRTGWEE FMRKCIYAFF 

       310        320        330        340        350        360 
QPQGREPSYA RQLFQEVMTR GTASSPSYRF ILNDGTMLSA HTKCKLCYPQ SPDMQPFIMG 

       370        380        390        400        410        420 
IHIIDREHSG LSPQDDTNSG MSIPRVNPPV NPSISPAHGV ARSSSLPPSN SNMVSTRVNR 

       430        440        450        460        470        480 
QQSSDLHSSS HSNSSNSQGS FGCSPGNQIV AGVALNQGQA SSQSTNPPLN LNNSPMEGTG 

       490        500        510        520        530        540 
ISLAQFMSPR RQVSSGLATR PRMPNNSFPP NIPTLNSPVS MTSTACNNSN RSYSNIPVTS 

       550        560        570        580        590        600 
LQSMNEGPNN SVGFSAGSPV LRQMSSQNSP SRLNIQPAKA ESKDNKETAS ILNEMIQSDN 

       610        620        630        640        650        660 
SSNDGKPLDS GLLHNNDRLS DGDNKYSQTS HKLVQLLTTT AEQQLRHADI DTSCKEVLSC 

       670        680        690        700        710        720 
TGTSSSASAN SSSGSCPSSH SSLTERHKIL HRLLQEGSPS DITTLSVEPD KKDSASTSVS 

       730        740        750        760        770        780 
VTGQVPGNSG IKLELDASKK KESKDHQLLR YLLDKDEKDL RSTPNLSLDD VKVKVEKKEQ 

       790        800        810        820        830        840 
MDPCNTNPTP MTKPPPEEIK LESQSQFTAD LDQFDQLLPT LEKAAQLPGL CETERMDGAV 

       850        860        870        880        890        900 
TSVTIKSEIL PATLQSTTAR PTSRLNRLPE LELEAIDNQF GQPGTGDQIP WANNTVTAVN 

       910        920        930        940        950        960 
QNKPEDQCIS SQLDELLCPP TTVEGRNDEK ALLEQLVSFL SGKDETELAE LDRALGIDKL 

       970        980        990       1000       1010       1020 
VQGGGLDVLS ERFPPQQATP PLMMEERPNL YSQPYSSPSP TANLSGPFQG MVRQKPSLGT 

      1030       1040       1050       1060       1070       1080 
MPVQVTPPRG AFSPGMGMQP RQTLNRPPAA PNQLRLQLQQ RLQGQQQLIH QNRQAILNQF 

      1090       1100       1110       1120       1130       1140 
AANAPVGINM RSGMQQQITP QPPLNAQMLA QRQRELYSQQ HRRRQLIQQQ RAMLMRQQSF 

      1150       1160       1170       1180       1190       1200 
GNNLPPSSGL PVPMGNPRLP QGAPQQFPYP PNYGTNPGTP PASTSPFSQL AENPEATLGN 

      1210       1220       1230       1240       1250       1260 
RNSMVNRGMT GNMGGQFGTG INPQMQQNVF QYPGSGMVPQ GEANFAPSLS PGSSMVPMPI 

      1270       1280       1290       1300       1310       1320 
PPPQSSLLQQ TPPASGYQSP DMKAWQQGAM GNNNVFSQAV QNQPTPAQPG VYNNMSITVS 

      1330       1340       1350       1360       1370       1380 
MAGGNTNVQN MNPMMGQMQM SSLQMPGMNT VCPEQINDPA LRHTGLYCNQ LSSTDLLKTE 

      1390       1400       1410       1420       1430       1440 
ADGTQVQQVQ VFADVQCTVN LVGGDPYLNQ PGPLGTQKPT AGPQTPQAQQ KSLLQQLLTE

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References

[1]"Molecular cloning of nuclear receptor coactivator-1 gene in pig."
Yu D., Mneg H., Bai C., Zhao W., Wang Q.S., Pan Y.
DNA Seq. 17:79-82(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		DQ078269 mRNA. Translation: AAY82453.1.
RefSeqNP_001020399.1. NM_001025228.1.
UniGeneSsc.24299.

3D structure databases

ProteinModelPortalQ4PJW2.
SMRQ4PJW2. Positions 259-367, 920-974.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID574068.
KEGGssc:574068.

Organism-specific databases

CTD8648.

Phylogenomic databases

eggNOGNOG315556.
HOVERGENHBG052583.
KOK09101.

Family and domain databases

Gene3D4.10.280.10. 1 hit.
4.10.630.10. 2 hits.
InterProIPR011598. bHLH_dom.
IPR010011. DUF1518.
IPR009110. Nuc_rcpt_coact.
IPR014920. Nuc_rcpt_coact_Ncoa-typ.
IPR017426. Nuclear_rcpt_coactivator.
IPR000014. PAS.
IPR013767. PAS_fold.
IPR014935. SRC-1.
IPR008955. Src1_rcpt_coact.
[Graphical view]
PfamPF07469. DUF1518. 2 hits.
PF08815. Nuc_rec_co-act. 1 hit.
PF00989. PAS. 1 hit.
PF08832. SRC-1. 1 hit.
[Graphical view]
PIRSFPIRSF038181. Nuclear_receptor_coactivator. 1 hit.
SMARTSM00353. HLH. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]
SUPFAMSSF47459. HLH_basic. 1 hit.
SSF69125. Nuc_recept_coact. 1 hit.
SSF55785. SSF55785. 2 hits.
PROSITEPS50888. BHLH. 1 hit.
PS50112. PAS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNCOA1_PIG
AccessionPrimary (citable) accession number: Q4PJW2
Entry history
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: July 19, 2005
Last modified: May 29, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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