Q4PHZ2 (ATG15_USTMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative lipase ATG15
EC=3.1.1.3

Alternative name(s):
Autophagy-related protein 15

Gene names

Name:	ATG15
ORF Names:	UM00271

Organism

Ustilago maydis (strain 521 / FGSC 9021) (Smut fungus)

Taxonomic identifier

237631 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Ustilaginomycotina › Ustilaginomycetes › Ustilaginales › Ustilaginaceae › Ustilago

Protein attributes

Sequence length	517 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	May be involved in lysis of subvacuolar cytoplasm to vacuole targeted bodies, intravacuolar autophagic bodies and of intravacuolar multivesicular body (MVB) vesicles By similarity.
Catalytic activity	Triacylglycerol + H2O = diacylglycerol + a carboxylate.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type II membrane protein By similarity. Golgi apparatus membrane; Single-pass type II membrane protein By similarity. Endosome › multivesicular body membrane; Single-pass type II membrane protein By similarity. Prevacuolar compartment membrane; Single-pass type II membrane protein By similarity. Note: From ER, targeted to vacuolar lumen at the MVB vesicles via the Golgi and the prevacuolar compartment (PVC) By similarity.
Sequence similarities	Belongs to the AB hydrolase superfamily. Lipase family.

Ontologies

Keywords
Biological process	Autophagy Lipid degradation
Cellular component	Endoplasmic reticulum Endosome Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	autophagy Inferred from electronic annotation. Source: UniProtKB-KW lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW multivesicular body membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	triglyceride lipase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 517	517	Putative lipase ATG15		PRO_0000317972
Regions
	Topological domain	1 – 6	6	Cytoplasmic By similarity
	Transmembrane	7 – 27	21	Helical; Signal-anchor for type II membrane protein
	Topological domain	28 – 517	490	Lumenal By similarity
Sites
	Active site	319	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	187	1	N-linked (GlcNAc...) Potential
	Glycosylation	221	1	N-linked (GlcNAc...) Potential
	Glycosylation	303	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q4PHZ2 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: 1D8C83DA988F10A0	FASTA	517	57,019
	10 20 30 40 50 60 MRASTHSWLL LVVVLSLSSF TVNAVILEGL IPPRSHLAPS TFQQPFALSS YLQKLFGTSL 70 80 90 100 110 120 RALYGAEQNR QTSVKTFGLR EAYHQGIGDK AHERARATFD FRITANDVQA DQANIDFIPA 130 140 150 160 170 180 IRTVRQRITR AKDPKKYVEV RGQSYRDAMC AASTELDWED VDVEAPDVTE RTTVLAFAKM 190 200 210 220 230 240 ASAAYKNDTS DWDGIGGFDP TNSFGWEGDG IRGHIFTTHD NDTIVVALKG TSNVFLGGGD 250 260 270 280 290 300 TARRDKTNDN LLFSCCCARV SWSWSTVCDC YQGHGDQCGQ TCVERALIEK SLYYPAITDL 310 320 330 340 350 360 FNNVSYAYPD SQIWITGHSL GGALSSLLGM TFGVPTVTFQ APGERMAAMR LHLPLPPAKH 370 380 390 400 410 420 ADESPVAALP IVHVYNNADP IATGTCNGAA SVCSNFGYAM ESKCHSGKSI VYDTVGKLGW 430 440 450 460 470 480 SMTINAHRIN TLVDDVLTED WSEKVRKKKA KLRSIGSRGG QISTRGWRWP WHRGDSADDD 490 500 510 GDSDEDTDED DKLAVPKARS EERCQDCTNW HFTDETL

References

[1]

"Insights from the genome of the biotrophic fungal plant pathogen Ustilago maydis." Kaemper J., Kahmann R., Boelker M., Ma L.-J., Brefort T., Saville B.J., Banuett F., Kronstad J.W., Gold S.E., Mueller O., Perlin M.H., Woesten H.A.B., de Vries R., Ruiz-Herrera J., Reynaga-Pena C.G., Snetselaar K., McCann M., Perez-Martin J. , Feldbruegge M., Basse C.W., Steinberg G., Ibeas J.I., Holloman W., Guzman P., Farman M.L., Stajich J.E., Sentandreu R., Gonzalez-Prieto J.M., Kennell J.C., Molina L., Schirawski J., Mendoza-Mendoza A., Greilinger D., Muench K., Roessel N., Scherer M., Vranes M., Ladendorf O., Vincon V., Fuchs U., Sandrock B., Meng S., Ho E.C.H., Cahill M.J., Boyce K.J., Klose J., Klosterman S.J., Deelstra H.J., Ortiz-Castellanos L., Li W., Sanchez-Alonso P., Schreier P.H., Haeuser-Hahn I., Vaupel M., Koopmann E., Friedrich G., Voss H., Schlueter T., Margolis J., Platt D., Swimmer C., Gnirke A., Chen F., Vysotskaia V., Mannhaupt G., Gueldener U., Muensterkoetter M., Haase D., Oesterheld M., Mewes H.-W., Mauceli E.W., DeCaprio D., Wade C.M., Butler J., Young S.K., Jaffe D.B., Calvo S.E., Nusbaum C., Galagan J.E., Birren B.W. Nature 444:97-101(2006) [PubMed: 17080091] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 521 / FGSC 9021.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AACP01000005 Genomic DNA. Translation: EAK81256.1.
RefSeq	XP_756418.1. XM_751325.1.
3D structure databases
ProteinModelPortal	Q4PHZ2.
ModBase	Search...
Protein-protein interaction databases
STRING	Q4PHZ2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	UM00271T0; UM00271P0; UM00271.
GeneID	3628352.
KEGG	uma:UM00271.1.
Phylogenomic databases
eggNOG	fuNOG04745.
OMA	FRITAND.
OrthoDB	EOG4F7RV2.
Family and domain databases
InterPro	IPR002921. Lipase_3. [Graphical view]
Pfam	PF01764. Lipase_3. 1 hit. [Graphical view]
PROSITE	PS00120. LIPASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ATG15_USTMA

Accession

Primary (citable) accession number: Q4PHZ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	July 19, 2005
Last modified:	December 14, 2011
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program