Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

S-methyl-5'-thioadenosine phosphorylase

Gene

UMAG_10466

Organism
Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.UniRule annotation

Catalytic activityi

S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation

Pathwayi: L-methionine biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. S-methyl-5'-thioadenosine phosphorylase (UMAG_10466)
This subpathway is part of the pathway L-methionine biosynthesis via salvage pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route), the pathway L-methionine biosynthesis via salvage pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei22PhosphateUniRule annotation1
Sitei187Important for substrate specificityUniRule annotation1
Binding sitei205Substrate; via amide nitrogenUniRule annotation1
Binding sitei206PhosphateUniRule annotation1
Sitei242Important for substrate specificityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processPurine salvage

Enzyme and pathway databases

UniPathwayiUPA00904; UER00873

Names & Taxonomyi

Protein namesi
Recommended name:
S-methyl-5'-thioadenosine phosphorylaseUniRule annotation (EC:2.4.2.28UniRule annotation)
Alternative name(s):
5'-methylthioadenosine phosphorylaseUniRule annotation
Short name:
MTA phosphorylaseUniRule annotation
Short name:
MTAPUniRule annotation
Short name:
MTAPaseUniRule annotation
Gene namesi
ORF Names:UMAG_10466
OrganismiUstilago maydis (strain 521 / FGSC 9021) (Corn smut fungus)
Taxonomic identifieri237631 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaUstilaginomycotinaUstilaginomycetesUstilaginalesUstilaginaceaeUstilago
Proteomesi
  • UP000000561 Componentsi: Chromosome 1, Unassembled WGS sequence

Organism-specific databases

EuPathDBiFungiDB:UMAG_10466

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004151371 – 315S-methyl-5'-thioadenosine phosphorylaseAdd BLAST315

Interactioni

Subunit structurei

Homotrimer.UniRule annotation

Protein-protein interaction databases

STRINGi5270.UM00570P0

Structurei

3D structure databases

SMRiQ4PH43
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni65 – 66Phosphate bindingUniRule annotation2
Regioni98 – 99Phosphate bindingUniRule annotation2
Regioni229 – 231Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.UniRule annotation

Phylogenomic databases

InParanoidiQ4PH43
KOiK00772
OrthoDBiEOG092C46SL

Family and domain databases

HAMAPiMF_01963 MTAP, 1 hit
InterProiView protein in InterPro
IPR010044 MTAP
IPR000845 Nucleoside_phosphorylase_d
IPR035994 Nucleoside_phosphorylase_sf
IPR018099 Purine_phosphorylase-2_CS
PANTHERiPTHR42679 PTHR42679, 1 hit
PfamiView protein in Pfam
PF01048 PNP_UDP_1, 1 hit
SUPFAMiSSF53167 SSF53167, 1 hit
TIGRFAMsiTIGR01694 MTAP, 1 hit
PROSITEiView protein in PROSITE
PS01240 PNP_MTAP_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q4PH43-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVPTVTSWT GAPILLGVIG GSGLYKLDSI TPVAEISIST PWGSASSPIT
60 70 80 90 100
IAKTSAGNHV AFLARHGRDH AILPSNVPNL ANIAALKHLG VKAIVAFSAV
110 120 130 140 150
GSLREEIAPK DFVIPSQIID RTKGVRRASF FGFGDEESVV AHAGFGDPFC
160 170 180 190 200
ETLRPIVYST VQATLASHPI KVHTDKTVVC MEGPQFSTRA ESLMYRTWGG
210 220 230 240 250
DIINMSVLPE AKLAREAEIA YVLIATATDY DAWRPSTAAV NVAEVMESLK
260 270 280 290 300
ANVEASNLVT TKVLDRVWLE IDTDEKPAVK NIKDSTKFSI MTKSQVIPDK
310
AKQNLRFLHP WFARD
Length:315
Mass (Da):34,081
Last modified:September 16, 2015 - v3
Checksum:iC303D082CD0947CE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CM003140 Genomic DNA Translation: KIS72152.1
RefSeqiXP_011386687.1, XM_011388385.1

Genome annotation databases

EnsemblFungiiKIS72152; KIS72152; UMAG_10466
GeneIDi23566497
KEGGiuma:UMAG_10466

Similar proteinsi

Entry informationi

Entry nameiMTAP_USTMA
AccessioniPrimary (citable) accession number: Q4PH43
Secondary accession number(s): A0A0D1CGT5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: September 16, 2015
Last modified: February 28, 2018
This is version 79 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome