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Protein

S-methyl-5'-thioadenosine phosphorylase

Gene

UM00570

Organism
Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.UniRule annotation

Catalytic activityi

S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei51 – 511PhosphateUniRule annotation
Sitei216 – 2161Important for substrate specificityUniRule annotation
Binding sitei234 – 2341Substrate; via amide nitrogenUniRule annotation
Binding sitei235 – 2351PhosphateUniRule annotation
Sitei271 – 2711Important for substrate specificityUniRule annotation

GO - Molecular functioni

  1. phosphorylase activity Source: InterPro
  2. S-methyl-5-thioadenosine phosphorylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. L-methionine biosynthetic process from methylthioadenosine Source: UniProtKB-HAMAP
  2. purine ribonucleoside salvage Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Enzyme and pathway databases

UniPathwayiUPA00904; UER00873.

Names & Taxonomyi

Protein namesi
Recommended name:
S-methyl-5'-thioadenosine phosphorylaseUniRule annotation (EC:2.4.2.28UniRule annotation)
Alternative name(s):
5'-methylthioadenosine phosphorylaseUniRule annotation
Short name:
MTA phosphorylaseUniRule annotation
Short name:
MTAPUniRule annotation
Short name:
MTAPaseUniRule annotation
Gene namesi
ORF Names:UM00570, UM10466
OrganismiUstilago maydis (strain 521 / FGSC 9021) (Corn smut fungus)
Taxonomic identifieri237631 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaUstilaginomycotinaUstilaginomycetesUstilaginalesUstilaginaceaeUstilago
ProteomesiUP000000561: Unassembled WGS sequence

Subcellular locationi

Cytoplasm UniRule annotation. Nucleus UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 344344S-methyl-5'-thioadenosine phosphorylasePRO_0000415137Add
BLAST

Interactioni

Subunit structurei

Homotrimer.UniRule annotation

Protein-protein interaction databases

STRINGi5270.UM00570.1.

Structurei

3D structure databases

ProteinModelPortaliQ4PH43.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni94 – 952Phosphate bindingUniRule annotation
Regioni127 – 1282Phosphate bindingUniRule annotation
Regioni258 – 2603Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0005.
InParanoidiQ4PH43.
KOiK00772.
OrthoDBiEOG77DJGM.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
HAMAPiMF_01963. MTAP.
InterProiIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
IPR018099. Purine_phosphorylase-2_CS.
[Graphical view]
PANTHERiPTHR11904. PTHR11904. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMiSSF53167. SSF53167. 1 hit.
TIGRFAMsiTIGR01694. MTAP. 1 hit.
PROSITEiPS01240. PNP_MTAP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4PH43-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLPRVSFVTT SLQQPNVSRC ISVRTLHRRM SVPTVTSWTG APILLGVIGG
60 70 80 90 100
SGLYKLDSIT PVAEISISTP WGSASSPITI AKTSAGNHVA FLARHGRDHA
110 120 130 140 150
ILPSNVPNLA NIAALKHLGV KAIVAFSAVG SLREEIAPKD FVIPSQIIDR
160 170 180 190 200
TKGVRRASFF GFGDEESVVA HAGFGDPFCE TLRPIVYSTV QATLASHPIK
210 220 230 240 250
VHTDKTVVCM EGPQFSTRAE SLMYRTWGGD IINMSVLPEA KLAREAEIAY
260 270 280 290 300
VLIATATDYD AWRPSTAAVN VAEVMESLKA NVEASNLVTT KVLDRVWLEI
310 320 330 340
DTDEKPAVKN IKDSTKFSIM TKSQVIPDKA KQNLRFLHPW FARD
Length:344
Mass (Da):37,446
Last modified:January 25, 2012 - v2
Checksum:i079237753E5CB5F3
GO

Sequence cautioni

The sequence EAK81219.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AACP01000013 Genomic DNA. Translation: EAK81219.1. Sequence problems.
RefSeqiXP_756717.1. XM_751624.1.

Genome annotation databases

EnsemblFungiiUM00570T0; UM00570P0; UM00570.
GeneIDi3628633.
KEGGiuma:UM00570.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AACP01000013 Genomic DNA. Translation: EAK81219.1. Sequence problems.
RefSeqiXP_756717.1. XM_751624.1.

3D structure databases

ProteinModelPortaliQ4PH43.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi5270.UM00570.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiUM00570T0; UM00570P0; UM00570.
GeneIDi3628633.
KEGGiuma:UM00570.1.

Phylogenomic databases

eggNOGiCOG0005.
InParanoidiQ4PH43.
KOiK00772.
OrthoDBiEOG77DJGM.

Enzyme and pathway databases

UniPathwayiUPA00904; UER00873.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
HAMAPiMF_01963. MTAP.
InterProiIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
IPR018099. Purine_phosphorylase-2_CS.
[Graphical view]
PANTHERiPTHR11904. PTHR11904. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMiSSF53167. SSF53167. 1 hit.
TIGRFAMsiTIGR01694. MTAP. 1 hit.
PROSITEiPS01240. PNP_MTAP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Insights from the genome of the biotrophic fungal plant pathogen Ustilago maydis."
    Kaemper J., Kahmann R., Boelker M., Ma L.-J., Brefort T., Saville B.J., Banuett F., Kronstad J.W., Gold S.E., Mueller O., Perlin M.H., Woesten H.A.B., de Vries R., Ruiz-Herrera J., Reynaga-Pena C.G., Snetselaar K., McCann M., Perez-Martin J.
    , Feldbruegge M., Basse C.W., Steinberg G., Ibeas J.I., Holloman W., Guzman P., Farman M.L., Stajich J.E., Sentandreu R., Gonzalez-Prieto J.M., Kennell J.C., Molina L., Schirawski J., Mendoza-Mendoza A., Greilinger D., Muench K., Roessel N., Scherer M., Vranes M., Ladendorf O., Vincon V., Fuchs U., Sandrock B., Meng S., Ho E.C.H., Cahill M.J., Boyce K.J., Klose J., Klosterman S.J., Deelstra H.J., Ortiz-Castellanos L., Li W., Sanchez-Alonso P., Schreier P.H., Haeuser-Hahn I., Vaupel M., Koopmann E., Friedrich G., Voss H., Schlueter T., Margolis J., Platt D., Swimmer C., Gnirke A., Chen F., Vysotskaia V., Mannhaupt G., Gueldener U., Muensterkoetter M., Haase D., Oesterheld M., Mewes H.-W., Mauceli E.W., DeCaprio D., Wade C.M., Butler J., Young S.K., Jaffe D.B., Calvo S.E., Nusbaum C., Galagan J.E., Birren B.W.
    Nature 444:97-101(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 521 / FGSC 9021.

Entry informationi

Entry nameiMTAP_USTMA
AccessioniPrimary (citable) accession number: Q4PH43
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: January 25, 2012
Last modified: January 7, 2015
This is version 57 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.