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Protein

Ubiquitin-conjugating enzyme E2 2

Gene

UBC2

Organism
Ustilago maydis (strain 521 / FGSC 9021) (Corn smut fungus)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the covalent attachment of ubiquitin to other proteins. Plays a role in transcription regulation by catalyzing the monoubiquitination of histone H2B to form H2BK123ub1. H2BK123ub1 gives a specific tag for epigenetic transcriptional activation and is also a prerequisite for H3K4me and H3K79me formation. Also involved in postreplication repair of UV-damaged DNA, in N-end rule-dependent protein degradation and in sporulation (By similarity).PROSITE-ProRule annotation

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei88 – 881Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ligase activity Source: UniProtKB-KW
  3. ubiquitin conjugating enzyme activity Source: EnsemblFungi

GO - Biological processi

  1. cellular response to hyperoxia Source: EnsemblFungi
  2. chromatin assembly Source: EnsemblFungi
  3. chromatin remodeling Source: EnsemblFungi
  4. chromatin silencing at silent mating-type cassette Source: EnsemblFungi
  5. chromatin silencing at telomere Source: EnsemblFungi
  6. DNA-templated transcription, termination Source: EnsemblFungi
  7. double-strand break repair via homologous recombination Source: EnsemblFungi
  8. ER-associated ubiquitin-dependent protein catabolic process Source: EnsemblFungi
  9. error-free postreplication DNA repair Source: EnsemblFungi
  10. error-free translesion synthesis Source: EnsemblFungi
  11. error-prone translesion synthesis Source: EnsemblFungi
  12. histone H2B conserved C-terminal lysine ubiquitination Source: EnsemblFungi
  13. histone monoubiquitination Source: EnsemblFungi
  14. meiotic DNA double-strand break formation Source: EnsemblFungi
  15. mitotic G1 DNA damage checkpoint Source: EnsemblFungi
  16. negative regulation of SREBP signaling pathway by positive regulation of transcription factor catabolic process in response to increased oxygen levels Source: EnsemblFungi
  17. negative regulation of transcription by transcription factor catabolism Source: EnsemblFungi
  18. protein polyubiquitination Source: EnsemblFungi
  19. protein ubiquitination involved in ubiquitin-dependent protein catabolic process Source: EnsemblFungi
  20. regulation of dipeptide transport Source: EnsemblFungi
  21. regulation of histone H3-K4 methylation Source: EnsemblFungi
  22. sporulation resulting in formation of a cellular spore Source: UniProtKB-KW
  23. telomere maintenance via recombination Source: EnsemblFungi
  24. transcription from RNA polymerase II promoter Source: EnsemblFungi
  25. ubiquitin-dependent protein catabolic process via the N-end rule pathway Source: EnsemblFungi
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Ligase

Keywords - Biological processi

DNA damage, DNA repair, Sporulation, Transcription, Transcription regulation, Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 2 (EC:6.3.2.19)
Alternative name(s):
Ubiquitin carrier protein UBC2
Ubiquitin-protein ligase UBC2
Gene namesi
Name:UBC2
ORF Names:UM01208
OrganismiUstilago maydis (strain 521 / FGSC 9021) (Corn smut fungus)
Taxonomic identifieri237631 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaUstilaginomycotinaUstilaginomycetesUstilaginalesUstilaginaceaeUstilago

Subcellular locationi

Cytoplasm By similarity. Nucleus By similarity

GO - Cellular componenti

  1. cytosol Source: EnsemblFungi
  2. HULC complex Source: EnsemblFungi
  3. nucleus Source: UniProtKB-SubCell
  4. Rad6-Rad18 complex Source: EnsemblFungi
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 185185Ubiquitin-conjugating enzyme E2 2PRO_0000082538Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi5270.UM01208.1.

Structurei

3D structure databases

ProteinModelPortaliQ4PFA5.
SMRiQ4PFA5. Positions 1-151.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5078.
HOGENOMiHOG000233454.
InParanoidiQ4PFA5.
KOiK10573.
OMAiPVPDNVM.
OrthoDBiEOG7SBP18.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4PFA5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTPSKKRLI RDFKRLSTDP PGGISGAPCA DNLMIWNAVI FGPADTPFED
60 70 80 90 100
GTFKLVLTFD ESYPNKPPTV KFLSKMFHPN VYANGELCLD ILQNRWSPTY
110 120 130 140 150
DVAAILTSIQ SLLHDPNPNS PANAEAASLY RENMKEYVRR VKATVEASWL
160 170 180
DDGEMPESIE EDDEAEAEAE AEATVDRSAP QTASA
Length:185
Mass (Da):20,411
Last modified:July 19, 2005 - v1
Checksum:i9D325AC43A9EE988
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AACP01000043 Genomic DNA. Translation: EAK81815.1.
RefSeqiXP_757355.1. XM_752262.1.

Genome annotation databases

EnsemblFungiiUM01208T0; UM01208P0; UM01208.
GeneIDi3629301.
KEGGiuma:UM01208.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AACP01000043 Genomic DNA. Translation: EAK81815.1.
RefSeqiXP_757355.1. XM_752262.1.

3D structure databases

ProteinModelPortaliQ4PFA5.
SMRiQ4PFA5. Positions 1-151.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi5270.UM01208.1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiUM01208T0; UM01208P0; UM01208.
GeneIDi3629301.
KEGGiuma:UM01208.1.

Phylogenomic databases

eggNOGiCOG5078.
HOGENOMiHOG000233454.
InParanoidiQ4PFA5.
KOiK10573.
OMAiPVPDNVM.
OrthoDBiEOG7SBP18.

Enzyme and pathway databases

UniPathwayiUPA00143.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Insights from the genome of the biotrophic fungal plant pathogen Ustilago maydis."
    Kaemper J., Kahmann R., Boelker M., Ma L.-J., Brefort T., Saville B.J., Banuett F., Kronstad J.W., Gold S.E., Mueller O., Perlin M.H., Woesten H.A.B., de Vries R., Ruiz-Herrera J., Reynaga-Pena C.G., Snetselaar K., McCann M., Perez-Martin J.
    , Feldbruegge M., Basse C.W., Steinberg G., Ibeas J.I., Holloman W., Guzman P., Farman M.L., Stajich J.E., Sentandreu R., Gonzalez-Prieto J.M., Kennell J.C., Molina L., Schirawski J., Mendoza-Mendoza A., Greilinger D., Muench K., Roessel N., Scherer M., Vranes M., Ladendorf O., Vincon V., Fuchs U., Sandrock B., Meng S., Ho E.C.H., Cahill M.J., Boyce K.J., Klose J., Klosterman S.J., Deelstra H.J., Ortiz-Castellanos L., Li W., Sanchez-Alonso P., Schreier P.H., Haeuser-Hahn I., Vaupel M., Koopmann E., Friedrich G., Voss H., Schlueter T., Margolis J., Platt D., Swimmer C., Gnirke A., Chen F., Vysotskaia V., Mannhaupt G., Gueldener U., Muensterkoetter M., Haase D., Oesterheld M., Mewes H.-W., Mauceli E.W., DeCaprio D., Wade C.M., Butler J., Young S.K., Jaffe D.B., Calvo S.E., Nusbaum C., Galagan J.E., Birren B.W.
    Nature 444:97-101(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 521 / FGSC 9021.

Entry informationi

Entry nameiUBC2_USTMA
AccessioniPrimary (citable) accession number: Q4PFA5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 10, 2006
Last sequence update: July 19, 2005
Last modified: April 1, 2015
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.