Reviewed, UniProtKB/Swiss-Prot Q4PBS8 (PMIP_USTMA)

Names and origin

Protein names

Recommended name:
    Mitochondrial intermediate peptidase
      Short name=MIP
    EC=3.4.24.59
Alternative name(s):
    Octapeptidyl aminopeptidase

Gene names

Name:	OCT1
ORF Names:	UM02435

Organism

Ustilago maydis (Smut fungus) [Complete proteome]

Taxonomic identifier

5270 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Ustilaginomycotina › Ustilaginomycetes › Ustilaginales › Ustilaginaceae › Ustilago

Protein attributes

Sequence length	863 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cleaves proteins, imported into the mitochondrion, to their mature size. While most mitochondrial precursor proteins are processed to the mature form in one step by mitochondrial processing peptidase (MPP), the sequential cleavage by MIP of an octapeptide after initial processing by MPP is a required step for a subgroup of nuclear-encoded precursor proteins destined for the matrix or the inner membrane By similarity.
Catalytic activity	Release of an N-terminal octapeptide as second stage of processing of some proteins imported into the mitochondrion.
Cofactor	Binds 1 zinc ion By similarity.
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the peptidase M3 family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 40	40	Mitochondrion Potential
	Chain	41 – 863	823	Mitochondrial intermediate peptidase		PRO_0000338595
Sites
	Active site	645	1	By similarity
	Metal binding	644	1	Zinc; catalytic By similarity
	Metal binding	648	1	Zinc; catalytic By similarity
	Metal binding	651	1	Zinc; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4PBS8-1 [UniParc]. Last modified June 10, 2008. Version 2. Checksum: 88FF232FA6308458	FASTA	863	95,030
	10 20 30 40 50 60 MSRLPTLLSN ISAPAASKAL DRYESKRIHS RSFSSSLAAQ RVQRPTSAGP ILTNPISDHE 70 80 90 100 110 120 KDNDELRSLF DAPPTSSSAN HLRSSGPSTG LFEIPSLTSP QNFLVLAQQT LARAQLLVDR 130 140 150 160 170 180 IDRAGSADAS TAQGIKELKE VVRNLDRLSD LLCGVIDMAE LVRNAHPDPE WAEAANAAYE 190 200 210 220 230 240 YLCGYMNVLN THTGLYSVLK NILSIKEVAE TLSKEATAVA QVFLRDFEKS GIHLPPAERE 250 260 270 280 290 300 RFVQLSDEIL VLGRGFLQDI AGNDASDDFA RIASAQADAD KSDMVGLPTH WLEDVNPTIL 310 320 330 340 350 360 KAVRASAITD TDGLLTFSAA DQPWVFQTLL KYAPDERARK VAFRAANYGS QAQVQRLERL 370 380 390 400 410 420 LKARAELATL TGASSYAEMA LGDKMAKEPQ NVEEFLRALT KHHRPRASHD LDKLRRLKHN 430 440 450 460 470 480 ATVSEPAQNT RQSTFNTNST LPEFAPWDRD MYTEQHFRSA SLSNVQPLSP YLSVGSVFAG 490 500 510 520 530 540 LSRLFSALYG IRFRASMVAP GEVWSEGAGD VMKVEVLDES EGARGTSGSA EGLIGTIYAD 550 560 570 580 590 600 LWSREGKPGG AAHYTVRCSR RVDKDDEAGD FTYGRAEDGR VVRPQDLGGE GCGNPLQAPT 610 620 630 640 650 660 FEQRERPGRY QLPVVVLMCD FARPGNANQG PCLLGWHEVE TLFHEMGHAI HSMIGRTSYH 670 680 690 700 710 720 NVSGTRCATD FVELPSILME HFVSSPQVVH LLARHHSTGA SLPFEHLSSH LAASKSLEGL 730 740 750 760 770 780 DTYHQILLAR LDQLYHSQLA ASPSFSSTTT YSDLDRQMHL PGAPNLSYTE GAHPQVRFGH 790 800 810 820 830 840 LFGYGSTYYS YLLDRVIASK VWNHLFANNP LDRYAGQVFK NQCLKYGGGK DPWHILADVL 850 860 NEDSVRQGDS RAMQQVGKWG IEC

References

[1]

"Insights from the genome of the biotrophic fungal plant pathogen Ustilago maydis." Kaemper J., Kahmann R., Boelker M., Ma L.-J., Brefort T., Saville B.J., Banuett F., Kronstad J.W., Gold S.E., Mueller O., Perlin M.H., Woesten H.A.B., de Vries R., Ruiz-Herrera J., Reynaga-Pena C.G., Snetselaar K., McCann M., Perez-Martin J. , Feldbruegge M., Basse C.W., Steinberg G., Ibeas J.I., Holloman W., Guzman P., Farman M.L., Stajich J.E., Sentandreu R., Gonzalez-Prieto J.M., Kennell J.C., Molina L., Schirawski J., Mendoza-Mendoza A., Greilinger D., Muench K., Roessel N., Scherer M., Vranes M., Ladendorf O., Vincon V., Fuchs U., Sandrock B., Meng S., Ho E.C.H., Cahill M.J., Boyce K.J., Klose J., Klosterman S.J., Deelstra H.J., Ortiz-Castellanos L., Li W., Sanchez-Alonso P., Schreier P.H., Haeuser-Hahn I., Vaupel M., Koopmann E., Friedrich G., Voss H., Schlueter T., Margolis J., Platt D., Swimmer C., Gnirke A., Chen F., Vysotskaia V., Mannhaupt G., Gueldener U., Muensterkoetter M., Haase D., Oesterheld M., Mewes H.-W., Mauceli E.W., DeCaprio D., Wade C.M., Butler J., Young S.K., Jaffe D.B., Calvo S.E., Nusbaum C., Galagan J.E., Birren B.W. Nature 444:97-101(2006) [PubMed: 17080091] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 521.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AACP01000083 Genomic DNA. Translation: EAK83473.1. Different initiation.
RefSeq	XP_758582.1.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	M03.006.
Genome annotation databases
GeneID	3630533.
KEGG	uma:UM02435.1.
Family and domain databases
InterPro	IPR001567. Pept_M3A_M3B. IPR006025. Pept_M_Zn_BS. [Graphical view]
Pfam	PF01432. Peptidase_M3. 1 hit. [Graphical view]
PROSITE	PS00142. ZINC_PROTEASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PMIP_USTMA

Accession

Primary (citable) accession number: Q4PBS8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 10, 2008
Last sequence update:	June 10, 2008
Last modified:	June 16, 2009
This is version 28 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)