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Q4PBS8 (PMIP_USTMA) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 51. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitochondrial intermediate peptidase

Short name=MIP
EC=3.4.24.59
Alternative name(s):
Octapeptidyl aminopeptidase
Gene names
Name:OCT1
ORF Names:UM02435
OrganismUstilago maydis (strain 521 / FGSC 9021) (Corn smut fungus) [Reference proteome]
Taxonomic identifier237631 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaBasidiomycotaUstilaginomycotinaUstilaginomycetesUstilaginalesUstilaginaceaeUstilago

Protein attributes

Sequence length863 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cleaves proteins, imported into the mitochondrion, to their mature size. While most mitochondrial precursor proteins are processed to the mature form in one step by mitochondrial processing peptidase (MPP), the sequential cleavage by MIP of an octapeptide after initial processing by MPP is a required step for a subgroup of nuclear-encoded precursor proteins destined for the matrix or the inner membrane By similarity.

Catalytic activity

Release of an N-terminal octapeptide as second stage of processing of some proteins imported into the mitochondrion.

Cofactor

Binds 1 zinc ion By similarity.

Subcellular location

Mitochondrion matrix By similarity.

Sequence similarities

Belongs to the peptidase M3 family.

Sequence caution

The sequence EAK83473.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Cellular componentMitochondrion
   DomainTransit peptide
   LigandMetal-binding
Zinc
   Molecular functionHydrolase
Metalloprotease
Protease
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentmitochondrial matrix

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionmetal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

metalloendopeptidase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 4040Mitochondrion Potential
Chain41 – 863823Mitochondrial intermediate peptidase
PRO_0000338595

Sites

Active site6451 By similarity
Metal binding6441Zinc; catalytic By similarity
Metal binding6481Zinc; catalytic By similarity
Metal binding6511Zinc; catalytic By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4PBS8 [UniParc].

Last modified June 10, 2008. Version 2.
Checksum: 88FF232FA6308458

FASTA86395,030
        10         20         30         40         50         60 
MSRLPTLLSN ISAPAASKAL DRYESKRIHS RSFSSSLAAQ RVQRPTSAGP ILTNPISDHE 

        70         80         90        100        110        120 
KDNDELRSLF DAPPTSSSAN HLRSSGPSTG LFEIPSLTSP QNFLVLAQQT LARAQLLVDR 

       130        140        150        160        170        180 
IDRAGSADAS TAQGIKELKE VVRNLDRLSD LLCGVIDMAE LVRNAHPDPE WAEAANAAYE 

       190        200        210        220        230        240 
YLCGYMNVLN THTGLYSVLK NILSIKEVAE TLSKEATAVA QVFLRDFEKS GIHLPPAERE 

       250        260        270        280        290        300 
RFVQLSDEIL VLGRGFLQDI AGNDASDDFA RIASAQADAD KSDMVGLPTH WLEDVNPTIL 

       310        320        330        340        350        360 
KAVRASAITD TDGLLTFSAA DQPWVFQTLL KYAPDERARK VAFRAANYGS QAQVQRLERL 

       370        380        390        400        410        420 
LKARAELATL TGASSYAEMA LGDKMAKEPQ NVEEFLRALT KHHRPRASHD LDKLRRLKHN 

       430        440        450        460        470        480 
ATVSEPAQNT RQSTFNTNST LPEFAPWDRD MYTEQHFRSA SLSNVQPLSP YLSVGSVFAG 

       490        500        510        520        530        540 
LSRLFSALYG IRFRASMVAP GEVWSEGAGD VMKVEVLDES EGARGTSGSA EGLIGTIYAD 

       550        560        570        580        590        600 
LWSREGKPGG AAHYTVRCSR RVDKDDEAGD FTYGRAEDGR VVRPQDLGGE GCGNPLQAPT 

       610        620        630        640        650        660 
FEQRERPGRY QLPVVVLMCD FARPGNANQG PCLLGWHEVE TLFHEMGHAI HSMIGRTSYH 

       670        680        690        700        710        720 
NVSGTRCATD FVELPSILME HFVSSPQVVH LLARHHSTGA SLPFEHLSSH LAASKSLEGL 

       730        740        750        760        770        780 
DTYHQILLAR LDQLYHSQLA ASPSFSSTTT YSDLDRQMHL PGAPNLSYTE GAHPQVRFGH 

       790        800        810        820        830        840 
LFGYGSTYYS YLLDRVIASK VWNHLFANNP LDRYAGQVFK NQCLKYGGGK DPWHILADVL 

       850        860 
NEDSVRQGDS RAMQQVGKWG IEC 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AACP01000083 Genomic DNA. Translation: EAK83473.1. Different initiation.
RefSeqXP_758582.1. XM_753489.1.

3D structure databases

ProteinModelPortalQ4PBS8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING5270.UM02435.1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3630533.
KEGGuma:UM02435.1.

Phylogenomic databases

eggNOGCOG0339.
HOGENOMHOG000076521.
KOK01410.
OrthoDBEOG71GB4R.

Family and domain databases

Gene3D1.10.1370.10. 2 hits.
1.20.1050.40. 1 hit.
3.40.390.10. 2 hits.
InterProIPR024079. MetalloPept_cat_dom.
IPR024077. Neurolysin/TOP_dom2.
IPR024080. Neurolysin/TOP_N.
IPR001567. Pept_M3A_M3B.
[Graphical view]
PfamPF01432. Peptidase_M3. 1 hit.
[Graphical view]
PROSITEPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePMIP_USTMA
AccessionPrimary (citable) accession number: Q4PBS8
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 10, 2008
Last modified: May 14, 2014
This is version 51 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries