Reviewed, UniProtKB/Swiss-Prot Q4PB56 (NMT_USTMA)

Names and origin

Protein names

Recommended name:
    Glycylpeptide N-tetradecanoyltransferase
    EC=2.3.1.97
Alternative name(s):
    Peptide N-myristoyltransferase
    Myristoyl-CoA:protein N-myristoyltransferase
      Short name=NMT

Gene names

Name:	NMT1
ORF Names:	UM02657

Organism

Ustilago maydis (Smut fungus) [Complete proteome]

Taxonomic identifier

5270 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Ustilaginomycotina › Ustilaginomycetes › Ustilaginales › Ustilaginaceae › Ustilago

Protein attributes

Sequence length	706 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Adds a myristoyl group to the N-terminal glycine residue of certain cellular proteins By similarity.
Catalytic activity	Tetradecanoyl-CoA + glycylpeptide = CoA + N-tetradecanoylglycylpeptide.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the NMT family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	N-terminal protein myristoylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycylpeptide N-tetradecanoyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 706	706	Glycylpeptide N-tetradecanoyltransferase		PRO_0000064245
Regions
	Region	180 – 183	4	Myristoyl CoA-binding By similarity
	Region	314 – 350	37	Myristoyl CoA-binding By similarity
Sites
	Active site	706	1	Proton acceptor; via carboxylate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4PB56-1 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: C2E3B38B6650D6F7	FASTA	706	77,562
	10 20 30 40 50 60 MSGIAGTSQD TSVAASASSS STRPAAASSS IAPPSPSLTT APVKEQEQDD DDDQENDDEE 70 80 90 100 110 120 EEEEGTAAIT GADGLTAKQR KKKKSKAAAK LRKKLGLGGS PTDASEGKLL ASGSSREGKI 130 140 150 160 170 180 SDEVVSQVQR AVQAEHGSVA ANAVTKANLA KVMAMMNLER DAMLKSQDSK QKAQKAIADH 190 200 210 220 230 240 KFWKTQPVMK PTDAPVVKSD QEGSIEASVP PEQVRQEPYP LPADFEWVMI DVDNEGELKE 250 260 270 280 290 300 VYDLLSANYV EDDDATFRFD YSPEFLHWVL KHPGYQKTWH IGVRVASTKK LVAFISGIPH 310 320 330 340 350 360 ELRVREKSYQ STEINFLCVH KKLRSKRLAP VLIKEVTRQC HLTGVFHAIY TVGSVLPTPV 370 380 390 400 410 420 SCSRYYHRTI NAKKLAEIGF SAIPHNMSME AHVKRFELPA KTNLPGLREM ERRDVAQVGK 430 440 450 460 470 480 LMRRYMRRFD MAPRFSDHEV EHILLSGRGE ACQGGRGRKG QVTWTYVVEN SEGRITDMFA 490 500 510 520 530 540 FYSLPSSILD SDKHQSLNAA YLFYYATDVV FQGDCDCDSD SSGVCDAETG RASTSQPSSE 550 560 570 580 590 600 RAVALAAGKA AWQCNSLTNL TSCELADEAR VAAWDSEPAH IKTALKTRLN LLINTLLIIA 610 620 630 640 650 660 RDHDFDVVNC VTVMDNALFL QQQKFGPGDG FLRFYLFNWR TKPVAGGMGA RPGEAELDPV 670 680 690 700 QAYARSMAKR AAHTDDDDVK RLAEQLIRNA AINPAEVGSG NGIVMV

References

[1]

"Insights from the genome of the biotrophic fungal plant pathogen Ustilago maydis." Kaemper J., Kahmann R., Boelker M., Ma L.-J., Brefort T., Saville B.J., Banuett F., Kronstad J.W., Gold S.E., Mueller O., Perlin M.H., Woesten H.A.B., de Vries R., Ruiz-Herrera J., Reynaga-Pena C.G., Snetselaar K., McCann M., Perez-Martin J. , Feldbruegge M., Basse C.W., Steinberg G., Ibeas J.I., Holloman W., Guzman P., Farman M.L., Stajich J.E., Sentandreu R., Gonzalez-Prieto J.M., Kennell J.C., Molina L., Schirawski J., Mendoza-Mendoza A., Greilinger D., Muench K., Roessel N., Scherer M., Vranes M., Ladendorf O., Vincon V., Fuchs U., Sandrock B., Meng S., Ho E.C.H., Cahill M.J., Boyce K.J., Klose J., Klosterman S.J., Deelstra H.J., Ortiz-Castellanos L., Li W., Sanchez-Alonso P., Schreier P.H., Haeuser-Hahn I., Vaupel M., Koopmann E., Friedrich G., Voss H., Schlueter T., Margolis J., Platt D., Swimmer C., Gnirke A., Chen F., Vysotskaia V., Mannhaupt G., Gueldener U., Muensterkoetter M., Haase D., Oesterheld M., Mewes H.-W., Mauceli E.W., DeCaprio D., Wade C.M., Butler J., Young S.K., Jaffe D.B., Calvo S.E., Nusbaum C., Galagan J.E., Birren B.W. Nature 444:97-101(2006) [PubMed: 17080091] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 521 / FGSC 9021.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AACP01000088 Genomic DNA. Translation: EAK83827.1.
RefSeq	XP_758804.1.
3D structure databases
SMR	Q4PB56. Positions 176-659.
ModBase	Search...
Protein-protein interaction databases
STRING	Q4PB56.
Genome annotation databases
GeneID	3630751.
KEGG	uma:UM02657.1.
Phylogenomic databases
eggNOG	fuNOG07746.
HOGENOM	HBG314670.
OMA	TKKLVAF.
OrthoDB	EOG95DZ5M.
PhylomeDB	Q4PB56.
Enzyme and pathway databases
BRENDA	2.3.1.97. 2320.
Family and domain databases
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR000903. Myristoyl_trans. [Graphical view]
Gene3D	G3DSA:3.40.630.30. Acyl_CoA_acyltransferase. 2 hits.
PANTHER	PTHR11377. Myristoyl_trans. 1 hit.
Pfam	PF01233. NMT. 1 hit. PF02799. NMT_C. 1 hit. [Graphical view]
PROSITE	PS00975. NMT_1. 1 hit. PS00976. NMT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NMT_USTMA

Accession

Primary (citable) accession number: Q4PB56

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	July 19, 2005
Last modified:	January 19, 2010
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)