Q4P9K9 (CHS8_USTMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chitin synthase 8
EC=2.4.1.16

Alternative name(s):
Chitin-UDP acetyl-glucosaminyl transferase 8
Myosin chitin synthase 1

Gene names

Name:	CHS8
Synonyms:	MCS1
ORF Names:	UM03204

Organism

Ustilago maydis (strain 521 / FGSC 9021) (Smut fungus)

Taxonomic identifier

237631 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Ustilaginomycotina › Ustilaginomycetes › Ustilaginales › Ustilaginaceae › Ustilago

Protein attributes

Sequence length	2005 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Plays a major role in cell wall biogenesis. Involved in mating tube and dikaryotic hyphae formation. Required for the formation of invading hyphae during plant infection. Essential for pathogenicity. Ref.3
Catalytic activity	UDP-N-acetyl-D-glucosamine + (1,4-(N-acetyl-beta-D-glucosaminyl))(n) = UDP + (1,4-(N-acetyl-beta-D-glucosaminyl))(n+1).
Subcellular location	Cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein. Note: A constitutive cytoplasmic pool is present that localizes to intracellular microvesicles termed chitosomes. Chitosomes constitute a separate secretory route distinct from the typical secretory pathway and serve as a vehicle for delivering the enzyme to the sites on the cell surface where polysaccharide sythesis takes place. Localizes to septa of yeast-like cells and to the basal septum separating the living tip cell from the vacuolated part in hyphae. Also localizes to the growing bud tip in yeast-like cells and in a tip-ward gradient at the hyphal apex. Ref.2 Ref.3
Sequence similarities	Belongs to the chitin synthase family. Class V subfamily. Contains 2 myosin head-like domains.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell membrane Cytoplasmic vesicle Membrane
Domain	Repeat Transmembrane Transmembrane helix
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasmic vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW myosin complex Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro chitin synthase activity Inferred from electronic annotation. Source: EC heme binding Inferred from electronic annotation. Source: InterPro motor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2005	2005	Chitin synthase 8		PRO_0000270624
Regions
	Transmembrane	929 – 949	21	Helical; Potential
	Transmembrane	965 – 985	21	Helical; Potential
	Transmembrane	1232 – 1252	21	Helical; Potential
	Transmembrane	1604 – 1624	21	Helical; Potential
	Transmembrane	1626 – 1646	21	Helical; Potential
	Transmembrane	1653 – 1673	21	Helical; Potential
	Transmembrane	1680 – 1700	21	Helical; Potential
	Domain	7 – 535	529	Myosin head-like 1
	Domain	629 – 692	64	Myosin head-like 2
	Compositional bias	614 – 621	8	Poly-Asp
	Compositional bias	1917 – 1926	10	Poly-Gln
Amino acid modifications
	Glycosylation	164	1	N-linked (GlcNAc...) Potential
	Glycosylation	364	1	N-linked (GlcNAc...) Potential
	Glycosylation	390	1	N-linked (GlcNAc...) Potential
	Glycosylation	546	1	N-linked (GlcNAc...) Potential
	Glycosylation	1076	1	N-linked (GlcNAc...) Potential
	Glycosylation	1650	1	N-linked (GlcNAc...) Potential
	Glycosylation	1770	1	N-linked (GlcNAc...) Potential
	Glycosylation	1794	1	N-linked (GlcNAc...) Potential
	Glycosylation	1882	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q4P9K9 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: EAB6EA732428DA10	FASTA	2,005	222,618
	10 20 30 40 50 60 MSALDEVAKL SQLTNITPDT IFSVLRDRFY AGLPYTALSD SILVSVNPYA SSGNRNSDDT 70 80 90 100 110 120 LREYTSDYRQ TNKQLRAATL PPHIFAHACN AYFYMRRTGQ DQSLLMAGDT SSGKSEVRRL 130 140 150 160 170 180 ALRALIDLSV APPGKKGSKL GVQIPSAEYI LEALGNSRTL ENSNASRFGK YTELQFSDSG 190 200 210 220 230 240 KLVGAKTLDY YLEKNRVVSA ASSERNFHIF HYMVAGASDE EKQYLGIHDA ASFRYLGQAS 250 260 270 280 290 300 RNMDTQDAAK FDRLKLAFKN VGFSKRNVAS ICQVLAAILH LGNIEFHYDR QRTQDSATIR 310 320 330 340 350 360 NPEVLDKVAE YLGISSKSLE EALTYKTKMI RNEVCTILLD ADGASDHRDD LAKSLYSLLF 370 380 390 400 410 420 AWVNESLNEK LCRDDFDTFI GLLDLPGFQN LSKGNSLDQF CVNFACENLH RFMLRSVFEK 430 440 450 460 470 480 RRDEFADEGI SHLSPEVPYF DNAETLRLMT NQPGGLIHIM DDQARRMPKK TDQTMIEAFG 490 500 510 520 530 540 KRWGNHPSFK VGPADRSGFS SFTISHYNSA VTYTSENLLE KNSEVVSTDF VSLLRGNPQE 550 560 570 580 590 600 SGKLRNDSSQ SSGSTIPFIR GIFNTKVLKT QSHPKNDQTI VAAQQSVKPM RAPSTRRPNR 610 620 630 640 650 660 GNTIKRTNTI KKADDDDSDE DAADAADAST SKKNAVRCVA GDFRGALDLL LETLEDTKTW 670 680 690 700 710 720 FTLCLRPNDN QLPNQFEARV VKQQITTLGL SEMSRKLLNE YSVSMTYEEF CQRYADVPSL 730 740 750 760 770 780 QAVQMRDAVS GEAKQKFSAA RQVMSWSDQE AVSGRVKVFL SHTAFRELED ELRAADAEEV 790 800 810 820 830 840 KNNEKRAQLD ADAAARGESD PFSPVAVLAD DYTRSRSNDF VGAYGDPFKE RSSVALPLVG 850 860 870 880 890 900 RGAAGNEDDL EEVKSQYSGM SGTLARHSFV GGLSGAPSFV ASEAYAPSRN MFADMGKNGL 910 920 930 940 950 960 NEKAGTAAFT EEPLGEVAEE VGVSSTRRKW VALTWAITFW IPSFILSRFR SLKRPDIRMA 970 980 990 1000 1010 1020 WREKLAINLI IWFICACAVF VIVVLGNLIC PKQHVYSPTE FASHKGDSSF TAIRGEVFDL 1030 1040 1050 1060 1070 1080 SNLVASHKTI VPVVPANSIL AYAGEDATPI FPVQVNALCN GVDGNVSPWV QLSNENSTDK 1090 1100 1110 1120 1130 1140 HAQYHDFRSY HIDDARPDWY YESMWLLRSN YRVGFMGYTT DGIRDILSEG RAVAIYRGDI 1150 1160 1170 1180 1190 1200 YDVSDYIKQG NQGVLRAPDG FQAPANTNRK FMSDAIISLI AQNPGKDITK QLDNLPLDPV 1210 1220 1230 1240 1250 1260 VLDRQRVCLR NLYFIGKVDH RNSPQCRFAQ YILLALSLFM VAILGFKFLA ALQFGRARKP 1270 1280 1290 1300 1310 1320 EDHDKFVICQ VPCYTEGEES MRKTINSLAA LKYDDKRKLL FIICDGMIVG SGNDRPTPRI 1330 1340 1350 1360 1370 1380 VLDILGADPN LEPEALSFLS LGEGSKQHNM AKIYSGLYEH HGHVVPYIVV VKCGKPSERS 1390 1400 1410 1420 1430 1440 RPGNRGKRDS QLVLMRFLNK VHFGLPMNPM ELEIYHQIKN VIGVNPSFYE YILQVDADTE 1450 1460 1470 1480 1490 1500 VEAMSLNRFI SAFIRDKKVI GLCGETALSN AKASIITMLQ VYEYYISHYL AKAFESLFGS 1510 1520 1530 1540 1550 1560 VTCLPGCFSM FRIRTPDTHR PLFIASQIVE DYAENRVDTL HTKNLLHLGE DRYLTTLVLK 1570 1580 1590 1600 1610 1620 HFGKYKTIFV RDCKAWTVAP DDWKVLLSQR RRWINSTVHN LVELIFTPGL CGFCLFSMRF 1630 1640 1650 1660 1670 1680 IVFIDLLSTI IAPVTVCYIV YLIVLVATAN GTVPLTAIIM LAAIYGCQAV IFLLNRKFEM 1690 1700 1710 1720 1730 1740 IGWMIVYIIG IPIWSLFLPL YSFWHMDDFS WGNTRVVMGE KGQKVVLHEE GTFDPSEIPL 1750 1760 1770 1780 1790 1800 QTWTDYENEL WERNSARSIG SIIEAARAEN KSLGSRAGSQ YAPSLYGQPM LPHNASFGHS 1810 1820 1830 1840 1850 1860 PSPSYGGTPS QFGAFAPGPG SQIGGSQIGA GAGYFPQDAA RQSTYSIGGG YGGQAMSMYG 1870 1880 1890 1900 1910 1920 LPPSSSFGVP TGGSGFMPQP FNTTASMYGY PQQVAATQSI YGGSQLGFGG GFATAEQQQQ 1930 1940 1950 1960 1970 1980 QQQQQQAAGL SGSGGSKSPP REAVAGGLPS DSQIKLDIRS LIAESDLTTI TKKQLRAKLE 1990 2000 QKYATSIESK KAFINSEIEN VLSES

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Insights from the genome of the biotrophic fungal plant pathogen Ustilago maydis." Kaemper J., Kahmann R., Boelker M., Ma L.-J., Brefort T., Saville B.J., Banuett F., Kronstad J.W., Gold S.E., Mueller O., Perlin M.H., Woesten H.A.B., de Vries R., Ruiz-Herrera J., Reynaga-Pena C.G., Snetselaar K., McCann M., Perez-Martin J. , Feldbruegge M., Basse C.W., Steinberg G., Ibeas J.I., Holloman W., Guzman P., Farman M.L., Stajich J.E., Sentandreu R., Gonzalez-Prieto J.M., Kennell J.C., Molina L., Schirawski J., Mendoza-Mendoza A., Greilinger D., Muench K., Roessel N., Scherer M., Vranes M., Ladendorf O., Vincon V., Fuchs U., Sandrock B., Meng S., Ho E.C.H., Cahill M.J., Boyce K.J., Klose J., Klosterman S.J., Deelstra H.J., Ortiz-Castellanos L., Li W., Sanchez-Alonso P., Schreier P.H., Haeuser-Hahn I., Vaupel M., Koopmann E., Friedrich G., Voss H., Schlueter T., Margolis J., Platt D., Swimmer C., Gnirke A., Chen F., Vysotskaia V., Mannhaupt G., Gueldener U., Muensterkoetter M., Haase D., Oesterheld M., Mewes H.-W., Mauceli E.W., DeCaprio D., Wade C.M., Butler J., Young S.K., Jaffe D.B., Calvo S.E., Nusbaum C., Galagan J.E., Birren B.W. Nature 444:97-101(2006) [PubMed: 17080091] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 521 / FGSC 9021.
[2]	"Immunolocalization of chitin synthases in the phytopathogenic dimorphic fungus Ustilago maydis." Ruiz-Herrera J., Xoconostle-Cazares B., Reynaga-Pena C.G., Leon-Ramirez C., Carabez-Trejo A. FEMS Yeast Res. 6:999-1009(2006) [PubMed: 17042749] [Abstract] Cited for: SUBCELLULAR LOCATION.
[3]	"Polar localizing class V myosin chitin synthases are essential during early plant infection in the plant pathogenic fungus Ustilago maydis." Weber I., Assmann D., Thines E., Steinberg G. Plant Cell 18:225-242(2006) [PubMed: 16314447] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AACP01000108 Genomic DNA. Translation: EAK84434.1.
RefSeq	XP_759351.1. XM_754258.1.
3D structure databases
HSSP	HSSP built from PDB template 1MND based on UniProtKB P08799.
ProteinModelPortal	Q4P9K9.
ModBase	Search...
Protein-protein interaction databases
STRING	Q4P9K9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	UM03204T0; UM03204P0; UM03204.
GeneID	3631285.
KEGG	uma:UM03204.1.
Phylogenomic databases
eggNOG	fuNOG06262.
HOGENOM	HBG739106.
OMA	FESLGEG.
OrthoDB	EOG4KM2B4.
Family and domain databases
InterPro	IPR004835. Chitin_synth_fng. IPR001199. Cyt_B5. IPR014876. DEK_C. IPR001609. Myosin_head_motor_dom. [Graphical view]
Gene3D	G3DSA:3.10.120.10. Cyt_B5. 1 hit.
KO	K00698.
Pfam	PF03142. Chitin_synth_2. 1 hit. PF00173. Cyt-b5. 1 hit. PF08766. DEK_C. 1 hit. PF00063. Myosin_head. 1 hit. [Graphical view]
PRINTS	PR00193. MYOSINHEAVY.
SMART	SM00242. MYSc. 1 hit. [Graphical view]
SUPFAM	SSF55856. Cyt_B5. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CHS8_USTMA

Accession

Primary (citable) accession number: Q4P9K9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	July 19, 2005
Last modified:	December 14, 2011
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program