Q4P763 (GUAA_USTMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GMP synthase [glutamine-hydrolyzing]
EC=6.3.5.2

Alternative name(s):
GMP synthetase
Glutamine amidotransferase

Gene names

Name:	GUA1
ORF Names:	UM04050

Organism

Ustilago maydis (strain 521 / FGSC 9021) (Smut fungus)

Taxonomic identifier

237631 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Ustilaginomycotina › Ustilaginomycetes › Ustilaginales › Ustilaginaceae › Ustilago

Protein attributes

Sequence length	543 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + xanthosine 5'-phosphate + L-glutamine + H2O = AMP + diphosphate + GMP + L-glutamate.
Pathway	Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain. Contains 1 GMPS ATP-PPase (ATP pyrophosphatase) domain.

Ontologies

Keywords
Biological process	GMP biosynthesis Purine biosynthesis
Cellular component	Cytoplasm
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	GMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GMP synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 543	543	GMP synthase [glutamine-hydrolyzing]		PRO_0000286155
Regions
	Domain	11 – 204	194	Glutamine amidotransferase type-1
	Domain	205 – 418	214	GMPS ATP-PPase
	Nucleotide binding	233 – 239	7	ATP By similarity
Sites
	Active site	87	1	For GATase activity By similarity
	Active site	179	1	For GATase activity By similarity
	Active site	181	1	For GATase activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4P763 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: 7421D2938981321A	FASTA	543	60,291
	10 20 30 40 50 60 MTEAIHSQYD SILILDFGSQ YSHLITRRCR ELNVYCEMLP CTQKIKDLDW KPKGIILSGS 70 80 90 100 110 120 PYSVYDEVAP RVDPDVFTAG VPVLGICYGL QEIAWNHGGK VDPHDKREYG HAMVEVVKHG 130 140 150 160 170 180 VPHLDALFKN WEGSVQVWMS HGDQLSKAPE DFVVIAKTPT APFAAMAHKS KPIYGVQFHP 190 200 210 220 230 240 EVTHSLRGVE LFDSFVDICA CRRDWTMETF IDKEIKRIRE IVGPKGQVLG AISGGVDSSV 250 260 270 280 290 300 AAKLMHEAIG DRFHAVMVDN GVLRTNEAAQ VYEMLSKDLG VNLTVVDASE QFLSRLKDVE 310 320 330 340 350 360 DPEQKRKIIG NTFIHVFEAE VAKLEKQAED EEAAALAAGK PQEAKGKFEY LLQGTLYPDV 370 380 390 400 410 420 IESISFKGPS ATIKTHHNVG GLLEDMKLKL IEPLRELFKD EVRALGKLLG IPAHLVGRHP 430 440 450 460 470 480 FPGPGLAIRI LGPVTREQVK ILQHADSIYI DEIRAAGLYD QISQAFAVLL PVRAVGVQGD 490 500 510 520 530 540 KRTYDQVIAL RAAATTDFMT ATWYPFPAEF LSKVSNRITN EVQGVNRVVL DISSKPPATI EWL

References

[1]

"Insights from the genome of the biotrophic fungal plant pathogen Ustilago maydis." Kaemper J., Kahmann R., Boelker M., Ma L.-J., Brefort T., Saville B.J., Banuett F., Kronstad J.W., Gold S.E., Mueller O., Perlin M.H., Woesten H.A.B., de Vries R., Ruiz-Herrera J., Reynaga-Pena C.G., Snetselaar K., McCann M., Perez-Martin J. , Feldbruegge M., Basse C.W., Steinberg G., Ibeas J.I., Holloman W., Guzman P., Farman M.L., Stajich J.E., Sentandreu R., Gonzalez-Prieto J.M., Kennell J.C., Molina L., Schirawski J., Mendoza-Mendoza A., Greilinger D., Muench K., Roessel N., Scherer M., Vranes M., Ladendorf O., Vincon V., Fuchs U., Sandrock B., Meng S., Ho E.C.H., Cahill M.J., Boyce K.J., Klose J., Klosterman S.J., Deelstra H.J., Ortiz-Castellanos L., Li W., Sanchez-Alonso P., Schreier P.H., Haeuser-Hahn I., Vaupel M., Koopmann E., Friedrich G., Voss H., Schlueter T., Margolis J., Platt D., Swimmer C., Gnirke A., Chen F., Vysotskaia V., Mannhaupt G., Gueldener U., Muensterkoetter M., Haase D., Oesterheld M., Mewes H.-W., Mauceli E.W., DeCaprio D., Wade C.M., Butler J., Young S.K., Jaffe D.B., Calvo S.E., Nusbaum C., Galagan J.E., Birren B.W. Nature 444:97-101(2006) [PubMed: 17080091] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 521 / FGSC 9021.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AACP01000141 Genomic DNA. Translation: EAK84975.1.
RefSeq	XP_760197.1. XM_755104.1.
3D structure databases
HSSP	HSSP built from PDB template 1GPM based on UniProtKB P04079.
ProteinModelPortal	Q4P763.
ModBase	Search...
Protein-protein interaction databases
STRING	Q4P763.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	UM04050T0; UM04050P0; UM04050.
GeneID	3632157.
KEGG	uma:UM04050.1.
Phylogenomic databases
eggNOG	fuNOG07551.
HOGENOM	HBG391775.
OMA	DGRTYEY.
OrthoDB	EOG4WWVST.
Family and domain databases
InterPro	IPR017926. GATASE_1. IPR001674. GMP_synth_C. IPR004739. GMP_synth_N. IPR022955. GMP_synthase. IPR022310. NAD/GMP_synthase. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01951.
Pfam	PF00117. GATase. 1 hit. PF00958. GMP_synt_C. 1 hit. PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00884. GuaA_Cterm. 1 hit. TIGR00888. GuaA_Nterm. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. PS51553. GMPS_ATP_PPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GUAA_USTMA

Accession

Primary (citable) accession number: Q4P763

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 1, 2007
Last sequence update:	July 19, 2005
Last modified:	January 25, 2012
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program