Reviewed, UniProtKB/Swiss-Prot Q4P5U1 (JHD1_USTMA)

Names and origin

Protein names

Recommended name:
    JmjC domain-containing histone demethylation protein 1
    EC=1.14.11.27
Alternative name(s):
    [Histone-H3]-lysine-36 demethylase 1

Gene names

Name:	JHD1
ORF Names:	UM04522

Organism

Ustilago maydis (Smut fungus) [Complete proteome]

Taxonomic identifier

5270 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Ustilaginomycotina › Ustilaginomycetes › Ustilaginales › Ustilaginaceae › Ustilago

Protein attributes

Sequence length	669 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Histone demethylase that specifically demethylates 'Lys-36' of histone H3, thereby playing a central role in histone code By similarity.
Catalytic activity	Protein N6,N(6)-dimethyl-L-lysine + 2-oxoglutarate + O2 = protein N(6)-methyl-L-lysine + succinate + formaldehyde + CO2. Protein N(6)-methyl-L-lysine + 2-oxoglutarate + O2 = protein L-lysine + succinate + formaldehyde + CO2.
Cofactor	Binds 1 Fe2+ ion per subunit By similarity.
Subcellular location	Nucleus By similarity.
Domain	The JmjC domain mediates the demethylation activity By similarity.
Sequence similarities	Belongs to the JHDM1 histone demethylase family. Contains 1 JmjC domain. Contains 1 PHD-type zinc finger.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Zinc-finger
Ligand	Iron Metal-binding Zinc
Molecular function	Chromatin regulator Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chromatin modification Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	histone demethylase activity (H3-K36 specific) Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 669	669	JmjC domain-containing histone demethylation protein 1		PRO_0000226799
Regions
	Domain	332 – 494	163	JmjC
	Zinc finger	65 – 126	62	PHD-type
Sites
	Metal binding	390	1	Iron; catalytic By similarity
	Metal binding	392	1	Iron; catalytic By similarity
	Metal binding	462	1	Iron; catalytic By similarity
	Binding site	407	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4P5U1-1 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: D8C8BAB766646282	FASTA	669	74,838
	10 20 30 40 50 60 MTAVAASSRV SDPLAASSSA HPRTLRSSPR KRTTSHDSSI SPSAAQSKQL PRRKKPRTEL 70 80 90 100 110 120 VDESELDCAA CPAVGQPAPT PGKGAASDRE TWICCTHCKT WFHCICIGLE NPDDFSKWYC 130 140 150 160 170 180 QPCITRSEQT FESGTSSSHP PFANVVRPPR RKSERAKLQI DYAAIQEGIP ADPLGRWKNL 190 200 210 220 230 240 LNAYEFEPDQ FRRMQGHEWT FDWLLHDESA LKQPVLVPAP PDRSSQAPHV QAKAEDVAAS 250 260 270 280 290 300 PVPRPKPARA KKQATHRLVP CHTSIPGMVV PPPEMSIFDV ADIIGHDTPV EVIDVASQSS 310 320 330 340 350 360 SKASWTISEW AEYFNTPKEK KKKTLNVISL EVTGTPMQAY VEAPQLVRDL DWVTRDWPAE 370 380 390 400 410 420 RRDASCSENS WPKVQRYVLM GVEGAYSDWH IDFAGSSVYY HVIWGQKTFL FAPPTARNLA 430 440 450 460 470 480 AYKAWCSSTR QDFDWLGDHL HSLTRVDIGP GETMLIPSGW LHCVYTPKNT LVVGGNFLTD 490 500 510 520 530 540 WNVATQWKLV EIEEATKVPR KFRFPHLKRL SWFVAKGWND RLEPLAEFET LTKEEDQVLE 550 560 570 580 590 600 ESAQVSAQVD VGSLTEVVPP LKVLNNIELV LQSLSDDLEL IQDPYVAESG DERKVKQQKA 610 620 630 640 650 660 AREAIPTHHV GNIQKAEAML ASLRQRVDRA KSLADAVQSE RVCLTWEATR AKKAKAANGS AIKSRKARR

References

[1]

"Insights from the genome of the biotrophic fungal plant pathogen Ustilago maydis." Kaemper J., Kahmann R., Boelker M., Ma L.-J., Brefort T., Saville B.J., Banuett F., Kronstad J.W., Gold S.E., Mueller O., Perlin M.H., Woesten H.A.B., de Vries R., Ruiz-Herrera J., Reynaga-Pena C.G., Snetselaar K., McCann M., Perez-Martin J. , Feldbruegge M., Basse C.W., Steinberg G., Ibeas J.I., Holloman W., Guzman P., Farman M.L., Stajich J.E., Sentandreu R., Gonzalez-Prieto J.M., Kennell J.C., Molina L., Schirawski J., Mendoza-Mendoza A., Greilinger D., Muench K., Roessel N., Scherer M., Vranes M., Ladendorf O., Vincon V., Fuchs U., Sandrock B., Meng S., Ho E.C.H., Cahill M.J., Boyce K.J., Klose J., Klosterman S.J., Deelstra H.J., Ortiz-Castellanos L., Li W., Sanchez-Alonso P., Schreier P.H., Haeuser-Hahn I., Vaupel M., Koopmann E., Friedrich G., Voss H., Schlueter T., Margolis J., Platt D., Swimmer C., Gnirke A., Chen F., Vysotskaia V., Mannhaupt G., Gueldener U., Muensterkoetter M., Haase D., Oesterheld M., Mewes H.-W., Mauceli E.W., DeCaprio D., Wade C.M., Butler J., Young S.K., Jaffe D.B., Calvo S.E., Nusbaum C., Galagan J.E., Birren B.W. Nature 444:97-101(2006) [PubMed: 17080091] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 521 / FGSC 9021.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AACP01000161 Genomic DNA. Translation: EAK85404.1.
RefSeq	XP_760669.1.
3D structure databases
SMR	Q4P5U1. Positions 89-128, 181-522.
ModBase	Search...
Genome annotation databases
GeneID	3632625.
KEGG	uma:UM04522.1.
Phylogenomic databases
eggNOG	fuNOG04730.
OrthoDB	EOG9H47M1.
PhylomeDB	Q4P5U1.
Enzyme and pathway databases
BRENDA	1.14.11.27. 2320.
Family and domain databases
InterPro	IPR003347. TF_JmjC_AAH. IPR019786. Zinc_finger_PHD-type_CS. IPR011011. Znf_FYVE_PHD. IPR001965. Znf_PHD. [Graphical view]
SMART	SM00558. JmjC. 1 hit. SM00249. PHD. 1 hit. [Graphical view]
PROSITE	PS51184. JMJC. 1 hit. PS01359. ZF_PHD_1. 1 hit. PS50016. ZF_PHD_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

JHD1_USTMA

Accession

Primary (citable) accession number: Q4P5U1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	July 19, 2005
Last modified:	January 19, 2010
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)