Q4P4T2 (Q4P4T2_USTMA) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
mRNA-capping enzyme subunit alpha PIRNR PIRNR036959
EC=2.7.7.50 PIRNR PIRNR036959

Alternative name(s):
GTP--RNA guanylyltransferase PIRNR PIRNR036959
mRNA guanylyltransferase PIRNR PIRNR036959

Gene names

ORF Names:

UM04881.1 EMBL EAK86181.1

Organism

Ustilago maydis (strain 521 / FGSC 9021) (Smut fungus)

Taxonomic identifier

237631 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Ustilaginomycotina › Ustilaginomycetes › Ustilaginales › Ustilaginaceae › Ustilago

Protein attributes

Sequence length	460 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Second step of mRNA capping. Transfer of the GMP moiety of GTP to the 5'-end of RNA via an enzyme-GMP covalent reaction intermediate By similarity. PIRNR PIRNR036959
Catalytic activity	GTP + (5')pp-Pur-mRNA = diphosphate + G(5')ppp-Pur-mRNA. PIRNR PIRNR036959
Subunit structure	The mRNA-capping enzyme is composed of two separate chains alpha and beta, respectively a mRNA guanylyltransferase and an RNA 5'-triphosphatase By similarity. PIRNR PIRNR036959
Subcellular location	Nucleus By similarity PIRNR PIRNR036959.
Sequence similarities	Belongs to the eukaryotic GTase family. PIRNR PIRNR036959

Ontologies

Keywords
Biological process	mRNA capping PIRNR PIRNR036959 mRNA processing
Cellular component	Nucleus PIRNR PIRNR036959
Ligand	GTP-binding PIRNR PIRNR036959 Nucleotide-binding
Molecular function	Nucleotidyltransferase PIRNR PIRNR036959 Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mRNA cap methyltransferase complex Inferred from electronic annotation. Source: InterPro
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW mRNA guanylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q4P4T2 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: 7E2E692ED1D470DD	FASTA	460	52,098
	10 20 30 40 50 60 MAPPGHAASV PNVPGRKVED PQQLSYLRNH VRDLCGLKHA RFPGAQPVSF EKASIDLLQS 70 80 90 100 110 120 EDYWVCEKSD GQRVLILIVT PSSTGIQEVF LIDRKNEYYK VEGIVFPHHL PKDNSMRNHT 130 140 150 160 170 180 LMDGELVIDT DASGKKKLVL LLFDLIVLDR ELLANRPLSK RYGRLKIYVY PPYVDYLKRN 190 200 210 220 230 240 PAAAALRPFD VQVKNMDLAY GIDKVLYETV PNLLHGNDGL IFTCLNSGYM MGTDPKILKW 250 260 270 280 290 300 KPPNENTIDF KLILRFPPDL DRDPRGNLPN LSVMPFFELH HYLGNSESED YEFFDELWVE 310 320 330 340 350 360 PEEWRQMVES GEQFDDRIVE CVWMVDPKPA TEPYLSQGLS LPPRWRMMRI RNDKHHGNHC 370 380 390 400 410 420 SIVQKILKSI HDGVDADELV RAAPAIRAAW KTPEREKRRQ EAGQAPAPVS ASSQSQATAL 430 440 450 460 KTNGTQPYDP SLPFGGMAYQ GIRGKAPPLV SGGPTGLIRR

References

[1]

"Insights from the genome of the biotrophic fungal plant pathogen Ustilago maydis." Kaemper J., Kahmann R., Boelker M., Ma L.-J., Brefort T., Saville B.J., Banuett F., Kronstad J.W., Gold S.E., Mueller O., Perlin M.H., Woesten H.A.B., de Vries R., Ruiz-Herrera J., Reynaga-Pena C.G., Snetselaar K., McCann M., Perez-Martin J. , Feldbruegge M., Basse C.W., Steinberg G., Ibeas J.I., Holloman W., Guzman P., Farman M.L., Stajich J.E., Sentandreu R., Gonzalez-Prieto J.M., Kennell J.C., Molina L., Schirawski J., Mendoza-Mendoza A., Greilinger D., Muench K., Roessel N., Scherer M., Vranes M., Ladendorf O., Vincon V., Fuchs U., Sandrock B., Meng S., Ho E.C.H., Cahill M.J., Boyce K.J., Klose J., Klosterman S.J., Deelstra H.J., Ortiz-Castellanos L., Li W., Sanchez-Alonso P., Schreier P.H., Haeuser-Hahn I., Vaupel M., Koopmann E., Friedrich G., Voss H., Schlueter T., Margolis J., Platt D., Swimmer C., Gnirke A., Chen F., Vysotskaia V., Mannhaupt G., Gueldener U., Muensterkoetter M., Haase D., Oesterheld M., Mewes H.-W., Mauceli E.W., DeCaprio D., Wade C.M., Butler J., Young S.K., Jaffe D.B., Calvo S.E., Nusbaum C., Galagan J.E., Birren B.W. Nature 444:97-101(2006) [PubMed: 17080091] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 521 / FGSC 9021.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AACP01000177 Genomic DNA. Translation: EAK86181.1.
RefSeq	XP_761028.1. XM_755935.1.
3D structure databases
ProteinModelPortal	Q4P4T2.
ModBase	Search...
Protein-protein interaction databases
STRING	Q4P4T2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	UM04881T0; UM04881P0; UM04881.
GeneID	3632969.
KEGG	uma:UM04881.1.
Phylogenomic databases
eggNOG	fuNOG04646.
HOGENOM	HBG330574.
OMA	GSQPVSF.
OrthoDB	EOG4RV611.
Family and domain databases
InterPro	IPR001339. mRNA_cap_enzyme. IPR013846. mRNA_cap_enzyme_C. IPR017075. mRNA_capping_enz_asu. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K00987.
Pfam	PF03919. mRNA_cap_C. 1 hit. PF01331. mRNA_cap_enzyme. 1 hit. [Graphical view]
PIRSF	PIRSF036959. mRNA_cap_alpha. 1 hit.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

Q4P4T2_USTMA

Accession

Primary (citable) accession number: Q4P4T2

Entry history

Integrated into UniProtKB/TrEMBL:	July 19, 2005
Last sequence update:	July 19, 2005
Last modified:	January 25, 2012
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)