Reviewed, UniProtKB/Swiss-Prot Q4P333 (CHS7_USTMA)

Names and origin

Protein names

Recommended name:
    Chitin synthase 7
    EC=2.4.1.16
Alternative name(s):
    Chitin-UDP acetyl-glucosaminyl transferase 7

Gene names

Name:	CHS7
ORF Names:	UM05480

Organism

Ustilago maydis (Smut fungus) [Complete proteome]

Taxonomic identifier

5270 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Ustilaginomycotina › Ustilaginomycetes › Ustilaginales › Ustilaginaceae › Ustilago

Protein attributes

Sequence length	1273 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Plays a major role in cell wall biogenesis. Required for the proper morphology of yeast-like cells. Involved in mating tube and dikaryotic hyphae formation. Essential for pathogenicity. Ref.2
Catalytic activity	UDP-N-acetyl-D-glucosamine + (1,4-(N-acetyl-beta-D-glucosaminyl))(n) = UDP + (1,4-(N-acetyl-beta-D-glucosaminyl))(n+1).
Subcellular location	Cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein. Note: A constitutive cytoplasmic pool is present that localizes to intracellular microvesicles termed chitosomes. Chitosomes constitute a separate secretory route distinct from the typical secretory pathway and serve as a vehicle for delivering the enzyme to the sites on the cell surface where polysaccharide sythesis takes place By similarity. Localizes to septa of yeast-like cells and to the basal septum separating the living tip cell from the vacuolated part in hyphae. Also localizes to the growing bud tip in yeast-like cells and to the tip of the hyphae.
Sequence similarities	Belongs to the chitin synthase family. Class IV subfamily.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Cell membrane Cytoplasmic vesicle Membrane
Domain	Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasmic vesicle Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	chitin synthase activity Inferred from electronic annotation. Source: EC heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1273	1273	Chitin synthase 7		PRO_0000270623
Regions
	Transmembrane	80 – 100	21	Potential
	Transmembrane	118 – 138	21	Potential
	Transmembrane	361 – 381	21	Potential
	Transmembrane	821 – 841	21	Potential
	Transmembrane	858 – 878	21	Potential
	Transmembrane	882 – 902	21	Potential
	Compositional bias	1213 – 1243	31	Pro-rich
Amino acid modifications
	Glycosylation	22	1	N-linked (GlcNAc...) Potential
	Glycosylation	229	1	N-linked (GlcNAc...) Potential
	Glycosylation	253	1	N-linked (GlcNAc...) Potential
	Glycosylation	269	1	N-linked (GlcNAc...) Potential
	Glycosylation	980	1	N-linked (GlcNAc...) Potential
	Glycosylation	1070	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q4P333-1 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: 4CB36E1DB66DC706	FASTA	1,273	140,139
	10 20 30 40 50 60 MPAVERNAPF TKTFIRKPGQ RNDTSIPLHT EAPPPLRQPT RIARAKTLTR PERSQPQVPL 70 80 90 100 110 120 INPSSGGPGF SAASRSKHRF SWWTAFSLFV TFWAPSPLLS SCCGLKDKQS RQAWREKVSL 130 140 150 160 170 180 VFIAILLGGF IGFITMGLNA ALCPSASSHS PNTYSRIGTG GAILGVHGWA FDIAQAHHLP 190 200 210 220 230 240 EAPALFSLST SRPGSDISSL FARSTSDQSP ACRGTTAAYA ADASCVALNG TLLKDCPLGP 250 260 270 280 290 300 LSPATFAQYG MYNQTRKIGY GWEDVESANF SNFLVLDGVV LNMSPYLKAN PSPIAADVVD 310 320 330 340 350 360 LAIRQQLATS PHRGRDATIT FYTNPTTRNA IKCLTQKYVA GYIDKITPGC FISNLVLYCS 370 380 390 400 410 420 LVVILAIVLI RFFMAVWFAW FMAGRMSSPP RPSRRRRLAP NVLPEGAMIS LNSSGAAPWA 430 440 450 460 470 480 NKQRPPPSQP ARRRRDSAQS ATPSVPDSLS VHHIGDEPYV VCLVTAYSEN EEGISTTLTS 490 500 510 520 530 540 LSETHYSDQR KLLFVVADGM VTGSGESMST PDVCVSLLEA DPRFGTPIPM SFVSIASGKK 550 560 570 580 590 600 EHNMAMVYAG HYTRATGRRT PMVVVVKCGA PEEAADSKPG NRGKRDSQMI LMNFFQRVTY 610 620 630 640 650 660 NDRMTPLDYD LFRKVHTLMG VTPDFFELCL MVDADTMVYP KSMKTLTNCM MRDPMIMGAC 670 680 690 700 710 720 GETRIANKTQ SWVTMIQVYE YFISHHQAKA FESVFGGVTC LPGCFSMYRI KARKQTDDDW 730 740 750 760 770 780 VPIIVKPEVT REYSQSVVTT LHQKNLLLLG EDRFLTTTLL RTFPNRKMVF CPEARCKTEV 790 800 810 820 830 840 PHTFKMLLSQ RRRWINSTIH NLMELVLVRD LCGTFCFSMQ FVVFMDLLGT AVLPISIALT 850 860 870 880 890 900 YTLVVTYCLN PPHSFTEAIP LMLLVAVIGM PALLILLATR KVVYVLWMLI YLLALPVWNF 910 920 930 940 950 960 VLPVYSFWHF DDFSWGETRK VEGEAKQTGH GDEGGSATGN AVPLRRWEDW ERSRLRKKKR 970 980 990 1000 1010 1020 EEKRRRELER QFGSGFHNDN ASDGDPDRKE AGMPLSRPGS DSFSDSVTVS DFDDDKWGNQ 1030 1040 1050 1060 1070 1080 IGGYDETLPP PVQIVRHSVW IGDQEVIIDT EDMEKMLETG WDDKAFRARN LSSASSTNAL 1090 1100 1110 1120 1130 1140 LQAQQPQYPS AVNRNRLSQM GAFATKRDAP AVPEIPARYS MYVQNNGGGR PANGHGNSNG 1150 1160 1170 1180 1190 1200 HYEPGSYEME RTPSPGEYAS LIRGGAPSPC SPGFGPAQPY GHSSAVSGGA GQYSTGSHAR 1210 1220 1230 1240 1250 1260 QRSGGANAAY NQPHQHPPQP SQPPQPPQPA QPTRPGGAPA APPRGAGSQG SGFAGARPSN 1270 PTGRGRSYHD RFS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Insights from the genome of the biotrophic fungal plant pathogen Ustilago maydis." Kaemper J., Kahmann R., Boelker M., Ma L.-J., Brefort T., Saville B.J., Banuett F., Kronstad J.W., Gold S.E., Mueller O., Perlin M.H., Woesten H.A.B., de Vries R., Ruiz-Herrera J., Reynaga-Pena C.G., Snetselaar K., McCann M., Perez-Martin J. , Feldbruegge M., Basse C.W., Steinberg G., Ibeas J.I., Holloman W., Guzman P., Farman M.L., Stajich J.E., Sentandreu R., Gonzalez-Prieto J.M., Kennell J.C., Molina L., Schirawski J., Mendoza-Mendoza A., Greilinger D., Muench K., Roessel N., Scherer M., Vranes M., Ladendorf O., Vincon V., Fuchs U., Sandrock B., Meng S., Ho E.C.H., Cahill M.J., Boyce K.J., Klose J., Klosterman S.J., Deelstra H.J., Ortiz-Castellanos L., Li W., Sanchez-Alonso P., Schreier P.H., Haeuser-Hahn I., Vaupel M., Koopmann E., Friedrich G., Voss H., Schlueter T., Margolis J., Platt D., Swimmer C., Gnirke A., Chen F., Vysotskaia V., Mannhaupt G., Gueldener U., Muensterkoetter M., Haase D., Oesterheld M., Mewes H.-W., Mauceli E.W., DeCaprio D., Wade C.M., Butler J., Young S.K., Jaffe D.B., Calvo S.E., Nusbaum C., Galagan J.E., Birren B.W. Nature 444:97-101(2006) [PubMed: 17080091] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 521.
[2]	"Polar localizing class V myosin chitin synthases are essential during early plant infection in the plant pathogenic fungus Ustilago maydis." Weber I., Assmann D., Thines E., Steinberg G. Plant Cell 18:225-242(2006) [PubMed: 16314447] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AACP01000197 Genomic DNA. Translation: EAK86413.1.
RefSeq	XP_761627.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q4P333.
Genome annotation databases
GeneID	3633595.
KEGG	uma:UM05480.1.
Phylogenomic databases
OMA	ETRIANK.
Enzyme and pathway databases
BRENDA	2.4.1.16. 2320.
Family and domain databases
InterPro	IPR004835. Chitin_synth_fng. IPR001199. Cyt_B5. [Graphical view]
Pfam	PF03142. Chitin_synth_2. 1 hit. PF00173. Cyt-b5. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHS7_USTMA

Accession

Primary (citable) accession number: Q4P333

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	July 19, 2005
Last modified:	November 3, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)