Reviewed, UniProtKB/Swiss-Prot Q4P2W8 (DOT1_USTMA)

Names and origin

Protein names

Recommended name:
    Histone-lysine N-methyltransferase, H3 lysine-79 specific
    EC=2.1.1.43
Alternative name(s):
    Histone H3-K79 methyltransferase
    H3-K79-HMTase

Gene names

Name:	DOT1
ORF Names:	UM05545

Organism

Ustilago maydis (Smut fungus) [Complete proteome]

Taxonomic identifier

5270 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Ustilaginomycotina › Ustilaginomycetes › Ustilaginales › Ustilaginaceae › Ustilago

Protein attributes

Sequence length	798 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Histone methyltransferase that specifically methylates histone H3 to form H3K79me. This methylation is required for telomere silencing and for the pachytene checkpoint during the meiotic cell cycle by allowing the recruitment of RAD9 to double strand breaks. Nucleosomes are preferred as substrate compared to free histones By similarity.
Catalytic activity	S-adenosyl-L-methionine + histone L-lysine = S-adenosyl-L-homocysteine + histone N(6)-methyl-L-lysine.
Enzyme regulation	Ubiquitination of histone H2B to form H2BK123ub1 is required for efficient DOT1 methyltransferase activity on histone H3 By similarity.
Subcellular location	Nucleus By similarity.
Miscellaneous	In contrast to other lysine histone methyltransferase, it does not contain a SET domain, suggesting the existence of another mechanism for methylation of lysine residues of histones.
Sequence similarities	Belongs to the DOT1 family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Repeat
Ligand	S-adenosyl-L-methionine
Molecular function	Chromatin regulator Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chromatin modification Inferred from electronic annotation. Source: UniProtKB-KW regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	histone-lysine N-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 798	798	Histone-lysine N-methyltransferase, H3 lysine-79 specific		PRO_0000270616
Regions
	Motif	577 – 588	12	SAM-binding motif 1 By similarity
	Motif	657 – 666	10	SAM-binding motif 2 By similarity
Sites
	Binding site	581	1	S-adenosyl-L-homocysteine By similarity
	Binding site	605	1	S-adenosyl-L-homocysteine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4P2W8-1 [UniParc]. Last modified July 19, 2005. Version 1. Checksum: F57A29F57EB14019	FASTA	798	84,989
	10 20 30 40 50 60 MINFFGGKAP TAAQPASSTS TAVNGARFAS VSTSSSASAS AAGQPPLKKT KIAPSPVTVV 70 80 90 100 110 120 TTVKKKVAPP TSVEPRDPIA ALSGYNSKNA LPEEKRRRII EERLAAQRQK SIESSLEAQL 130 140 150 160 170 180 RAPTKSLSKS QSASSTSRRS AASFSKPKTK KPRKKVVDSD SDHDEFGDYG KSSRKPSTAP 190 200 210 220 230 240 STPRASKRTS VDPHPTSDSY QKVGRDGVQP NYTVPRDIRA STSPTNTTAI PIDTQSRPSK 250 260 270 280 290 300 PISSADIVTA NIKNYGPYFN GLGDAPRAVL EYPGTDASEE FLLLVPKDAD EYDPLMELLA 310 320 330 340 350 360 TVRAIVTHYL TPEQRKAFGS LDSLEVSNNA GMILPSATHG ASASTSAVCN MVAALTDSNG 370 380 390 400 410 420 INGARAKSAD LLDGQGSKAE RRNHLVGAGT YKDTGSMTPE PHANGISQTA PVSPTSQTSG 430 440 450 460 470 480 ISASAGGSSV AKTVLVNNAL LRLDSPAPTE LSVASDHSST PAASCGTDPD SILRSFTKAR 490 500 510 520 530 540 NRRDGPLFMR TLARFNSALA ALRDTGALAA NIADLGSCTG VPEGIWRLIQ DQVYARVVGP 550 560 570 580 590 600 RVEELGRYQA FSDNVYGELL PRFMSEIAQL TLLGPEKVFV DLGSGVGNLL IQTSLQTGAE 610 620 630 640 650 660 AYGCEMMRIP ASLASQQVVE AQLRWAAWGL RGGSAIEAWQ GDFGDHGGVR DVLKRADVVL 670 680 690 700 710 720 VNNYAFLPKT NENLSLLFLD LPDGAKVVSL KPFVPPDFRL TQRTLSSPLA ILRVTERLYT 730 740 750 760 770 780 SGCVSWADGG GKYYIQEVDR SLVREFLQNA GAATRAKRGK VSEGDDTVAA SDLMVGQDVR 790 RKRWKAALQD DDEDDDEF

References

[1]

"Insights from the genome of the biotrophic fungal plant pathogen Ustilago maydis." Kaemper J., Kahmann R., Boelker M., Ma L.-J., Brefort T., Saville B.J., Banuett F., Kronstad J.W., Gold S.E., Mueller O., Perlin M.H., Woesten H.A.B., de Vries R., Ruiz-Herrera J., Reynaga-Pena C.G., Snetselaar K., McCann M., Perez-Martin J. , Feldbruegge M., Basse C.W., Steinberg G., Ibeas J.I., Holloman W., Guzman P., Farman M.L., Stajich J.E., Sentandreu R., Gonzalez-Prieto J.M., Kennell J.C., Molina L., Schirawski J., Mendoza-Mendoza A., Greilinger D., Muench K., Roessel N., Scherer M., Vranes M., Ladendorf O., Vincon V., Fuchs U., Sandrock B., Meng S., Ho E.C.H., Cahill M.J., Boyce K.J., Klose J., Klosterman S.J., Deelstra H.J., Ortiz-Castellanos L., Li W., Sanchez-Alonso P., Schreier P.H., Haeuser-Hahn I., Vaupel M., Koopmann E., Friedrich G., Voss H., Schlueter T., Margolis J., Platt D., Swimmer C., Gnirke A., Chen F., Vysotskaia V., Mannhaupt G., Gueldener U., Muensterkoetter M., Haase D., Oesterheld M., Mewes H.-W., Mauceli E.W., DeCaprio D., Wade C.M., Butler J., Young S.K., Jaffe D.B., Calvo S.E., Nusbaum C., Galagan J.E., Birren B.W. Nature 444:97-101(2006) [PubMed: 17080091] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 521.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AACP01000200 Genomic DNA. Translation: EAK86305.1.
RefSeq	XP_761692.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3633676.
KEGG	uma:UM05545.1.
Enzyme and pathway databases
BRENDA	2.1.1.43. 2320.
Family and domain databases
InterPro	IPR013110. DOT1. [Graphical view]
Pfam	PF08123. DOT1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DOT1_USTMA

Accession

Primary (citable) accession number: Q4P2W8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 9, 2007
Last sequence update:	July 19, 2005
Last modified:	June 16, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)