Q4P0N0 (KMO_USTMA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Kynurenine 3-monooxygenase
EC=1.14.13.9

Alternative name(s):
Biosynthesis of nicotinic acid protein 4
Kynurenine 3-hydroxylase

Gene names

Name:	BNA4
ORF Names:	UM06333

Organism

Ustilago maydis (strain 521 / FGSC 9021) (Smut fungus)

Taxonomic identifier

237631 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Basidiomycota › Ustilaginomycotina › Ustilaginomycetes › Ustilaginales › Ustilaginaceae › Ustilago

Protein attributes

Sequence length	600 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the hydroxylation of L-kynurenine (L-Kyn) to form 3-hydroxy-L-kynurenine (L-3OHKyn). Required for synthesis of quinolinic acid By similarity.
Catalytic activity	L-kynurenine + NADPH + O2 = 3-hydroxy-L-kynurenine + NADP+ + H2O.
Cofactor	FAD By similarity.
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from L-kynurenine: step 1/3.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the aromatic-ring hydroxylase family. KMO subfamily.
Sequence caution	The sequence EAK82962.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Cellular component	Mitochondrion
Ligand	FAD Flavoprotein NADP
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	pyridine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro kynurenine 3-monooxygenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 600	600	Kynurenine 3-monooxygenase		PRO_0000361933

Sequences

	Sequence		Length	Mass (Da)
	Q4P0N0 [UniParc]. Last modified February 10, 2009. Version 2. Checksum: 52C6ECAF5FFC31A5	FASTA	600	65,460
	10 20 30 40 50 60 MSETRKDTDR ASAQLPTKCQ LPERVAVIGA GPVGCLAALA FAQRGCKVDI FESRPDPRTH 70 80 90 100 110 120 EAITRASQRS INLALSTRGI TGLRSVSLAG LGVGTNAPNG MDLADLVLQN SVPMRARMIH 130 140 150 160 170 180 VVTRQASATQ AAEVKEISQL YSTKGESINS VDRGRLNNIL LEHALMHRNV EVHFEHKLQS 190 200 210 220 230 240 VDFDHDIKSA AKRAGTPPPA IDASANKWLR GANVTGGDSC ADEPSGCGGR KQATTKSQGS 250 260 270 280 290 300 EYVAPAGTSA VDRVRLDFDV HSTNQHIRKT SNTHYASFVV GCDGAHSSIR SAMGSLIRMH 310 320 330 340 350 360 YTHNYIDTGY IELSIPPRTS LGSGSRIRGS GGVDGKRGGH DAFHLDANHL HIWPRHSFML 370 380 390 400 410 420 IALPNLDGSF TCTLFAPFKM FASELSTKEG IVAVFQQHFP DALALIDEEK LVKCLTTRRA 430 440 450 460 470 480 SALGSVQCDP YHYKDRAVLI GDAAHAMLPF YGQGLNCGFE DVRVMFDIID QHESLQVALD 490 500 510 520 530 540 LYTKERHPDL VAILQLAEQN YREMAHSVVS WPYLLRKKLD GLLMSILPSS MWSSLYAMTT 550 560 570 580 590 600 FSNLPYSRVV KTEKRQQAII GHALVATAVA FVSAAAVGVY STRALWQPVT VRCIARLHNS

References

[1]

"Insights from the genome of the biotrophic fungal plant pathogen Ustilago maydis." Kaemper J., Kahmann R., Boelker M., Ma L.-J., Brefort T., Saville B.J., Banuett F., Kronstad J.W., Gold S.E., Mueller O., Perlin M.H., Woesten H.A.B., de Vries R., Ruiz-Herrera J., Reynaga-Pena C.G., Snetselaar K., McCann M., Perez-Martin J. , Feldbruegge M., Basse C.W., Steinberg G., Ibeas J.I., Holloman W., Guzman P., Farman M.L., Stajich J.E., Sentandreu R., Gonzalez-Prieto J.M., Kennell J.C., Molina L., Schirawski J., Mendoza-Mendoza A., Greilinger D., Muench K., Roessel N., Scherer M., Vranes M., Ladendorf O., Vincon V., Fuchs U., Sandrock B., Meng S., Ho E.C.H., Cahill M.J., Boyce K.J., Klose J., Klosterman S.J., Deelstra H.J., Ortiz-Castellanos L., Li W., Sanchez-Alonso P., Schreier P.H., Haeuser-Hahn I., Vaupel M., Koopmann E., Friedrich G., Voss H., Schlueter T., Margolis J., Platt D., Swimmer C., Gnirke A., Chen F., Vysotskaia V., Mannhaupt G., Gueldener U., Muensterkoetter M., Haase D., Oesterheld M., Mewes H.-W., Mauceli E.W., DeCaprio D., Wade C.M., Butler J., Young S.K., Jaffe D.B., Calvo S.E., Nusbaum C., Galagan J.E., Birren B.W. Nature 444:97-101(2006) [PubMed: 17080091] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 521 / FGSC 9021.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AACP01000242 Genomic DNA. Translation: EAK82962.1. Sequence problems.
RefSeq	XP_762480.1. XM_757387.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q4P0N0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3634450.
KEGG	uma:UM06333.1.
Phylogenomic databases
eggNOG	fuNOG06151.
OrthoDB	EOG4QG0P2.
Family and domain databases
InterPro	IPR002938. mOase_FAD-bd. IPR001327. Pyr_OxRdtase_NAD-bd_dom. IPR003042. Rng_hydrolase-like. [Graphical view]
KO	K00486.
Pfam	PF01494. FAD_binding_3. 1 hit. PF00070. Pyr_redox. 1 hit. [Graphical view]
PRINTS	PR00420. RNGMNOXGNASE.
ProtoNet	Search...

Entry information

Entry name

KMO_USTMA

Accession

Primary (citable) accession number: Q4P0N0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	February 10, 2009
Last modified:	November 16, 2011
This is version 53 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program