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Q4LDG0

- S27A5_MOUSE

UniProt

Q4LDG0 - S27A5_MOUSE

Protein

Bile acyl-CoA synthetase

Gene

Slc27a5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 86 (01 Oct 2014)
      Sequence version 2 (22 Nov 2005)
      Previous versions | rss
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    Functioni

    Acyl-CoA synthetase involved in bile acid metabolism. Proposed to catalyze the first step in the conjugation of C24 bile acids (choloneates) to glycine and taurine before excretion into bile canaliculi by activating them to their CoA thioesters. Seems to activate secondary bile acids entering the liver from the enterohepatic circulation By similarity.By similarity

    Catalytic activityi

    ATP + cholate + CoA = AMP + diphosphate + choloyl-CoA.
    ATP + (25R)-3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestan-26-oate + CoA = AMP + diphosphate + (25R)-3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestanoyl-CoA.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi292 – 30312AMPSequence AnalysisAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cholate-CoA ligase activity Source: UniProtKB-EC
    3. fatty acid transporter activity Source: MGI
    4. long-chain fatty acid-CoA ligase activity Source: MGI
    5. very long-chain fatty acid-CoA ligase activity Source: Ensembl

    GO - Biological processi

    1. bile acid biosynthetic process Source: Ensembl
    2. bile acid metabolic process Source: MGI
    3. fatty acid transport Source: MGI
    4. ketone body biosynthetic process Source: MGI
    5. long-chain fatty acid metabolic process Source: MGI
    6. plasma membrane long-chain fatty acid transport Source: MGI
    7. triglyceride mobilization Source: MGI
    8. very long-chain fatty acid metabolic process Source: Ensembl

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Fatty acid metabolism, Lipid metabolism

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_203193. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
    REACT_210229. Synthesis of bile acids and bile salts via 24-hydroxycholesterol.

    Protein family/group databases

    TCDBi4.C.1.1.8. the proposed fatty acid transporter (fat) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bile acyl-CoA synthetase (EC:6.2.1.7)
    Short name:
    BACS
    Alternative name(s):
    Bile acid-CoA ligase
    Short name:
    BA-CoA ligase
    Short name:
    BAL
    Cholate--CoA ligase
    Fatty acid transport protein 5
    Short name:
    FATP-5
    Solute carrier family 27 member 5
    Very long-chain acyl-CoA synthetase-related protein
    Short name:
    VLACS-related
    Short name:
    VLACSR
    Gene namesi
    Name:Slc27a5
    Synonyms:Acsb, Acsvl6, Fatp5, Vlacsr
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:1347100. Slc27a5.

    Subcellular locationi

    GO - Cellular componenti

    1. basal plasma membrane Source: MGI
    2. endoplasmic reticulum Source: MGI
    3. integral component of endoplasmic reticulum membrane Source: Ensembl
    4. protein complex Source: Ensembl

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 689689Bile acyl-CoA synthetasePRO_0000193214Add
    BLAST

    Proteomic databases

    MaxQBiQ4LDG0.
    PaxDbiQ4LDG0.
    PRIDEiQ4LDG0.

    Expressioni

    Tissue specificityi

    In liver expressed in a periportal distribution.1 Publication

    Gene expression databases

    BgeeiQ4LDG0.
    GenevestigatoriQ4LDG0.

    Interactioni

    Protein-protein interaction databases

    IntActiQ4LDG0. 2 interactions.
    MINTiMINT-1863806.

    Structurei

    3D structure databases

    ProteinModelPortaliQ4LDG0.
    SMRiQ4LDG0. Positions 111-660.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 2929CytoplasmicBy similarityAdd
    BLAST
    Topological domaini76 – 689614CytoplasmicBy similarityAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei30 – 5021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei55 – 7521HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0318.
    GeneTreeiENSGT00550000074420.
    HOGENOMiHOG000044189.
    HOVERGENiHBG005642.
    InParanoidiA6H6C1.
    KOiK08748.
    OMAiPHWLSLA.
    OrthoDBiEOG7W6WKB.
    PhylomeDBiQ4LDG0.
    TreeFamiTF313430.

    Family and domain databases

    InterProiIPR025110. AMP-bd_C.
    IPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    [Graphical view]
    PfamiPF00501. AMP-binding. 1 hit.
    PF13193. AMP-binding_C. 1 hit.
    [Graphical view]
    PROSITEiPS00455. AMP_BINDING. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q4LDG0-1 [UniParc]FASTAAdd to Basket

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    MGIWKKLTLL LLLLLLVGLG QPPWPAAMAL ALRWFLGDPT CLVLLGLALL    50
    GRPWISSWMP HWLSLVGAAL TLFLLPLQPP PGLRWLHKDV AFTFKMLFYG 100
    LKFRRRLNKH PPETFVDALE RQALAWPDRV ALVCTGSEGS SITNSQLDAR 150
    SCQAAWVLKA KLKDAVIQNT RDAAAILVLP SKTISALSVF LGLAKLGCPV 200
    AWINPHSRGM PLLHSVRSSG ASVLIVDPDL QENLEEVLPK LLAENIHCFY 250
    LGHSSPTPGV EALGASLDAA PSDPVPASLR ATIKWKSPAI FIFTSGTTGL 300
    PKPAILSHER VIQVSNVLSF CGCRADDVVY DVLPLYHTIG LVLGFLGCLQ 350
    VGATCVLAPK FSASRFWAEC RQHGVTVILY VGEILRYLCN VPEQPEDKIH 400
    TVRLAMGNGL RANVWKNFQQ RFGPIRIWEF YGSTEGNVGL MNYVGHCGAV 450
    GRTSCILRML TPFELVQFDI ETAEPLRDKQ GFCIPVEPGK PGLLLTKVRK 500
    NQPFLGYRGS QAESNRKLVA NVRRVGDLYF NTGDVLTLDQ EGFFYFQDRL 550
    GDTFRWKGEN VSTGEVECVL SSLDFLEEVN VYGVPVPGCE GKVGMAAVKL 600
    APGKTFDGQK LYQHVRSWLP AYATPHFIRI QDSLEITNTY KLVKSRLVRE 650
    GFDVGIIADP LYILDNKAQT FRSLMPDVYQ AVCEGTWNL 689
    Length:689
    Mass (Da):76,203
    Last modified:November 22, 2005 - v2
    Checksum:i1642BBC2CF04FAA3
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti88 – 881K → I in CAA11688. (PubMed:9642112)Curated
    Sequence conflicti408 – 4081N → T in AAC40189. (PubMed:9671728)Curated
    Sequence conflicti568 – 5681C → S in CAA11688. (PubMed:9642112)Curated
    Sequence conflicti688 – 6881N → K in CAA11688. (PubMed:9642112)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ223959 mRNA. Translation: CAA11688.1.
    BC013335 mRNA. Translation: AAH13335.1.
    BC013272 mRNA. Translation: AAH13272.1.
    BC145823 mRNA. Translation: AAI45824.1.
    BC145825 mRNA. Translation: AAI45826.1.
    AF072760 mRNA. Translation: AAC40189.1.
    CCDSiCCDS20821.1.
    PIRiJW0107.
    RefSeqiNP_033538.2. NM_009512.2.
    UniGeneiMm.10984.

    Genome annotation databases

    EnsembliENSMUST00000032539; ENSMUSP00000032539; ENSMUSG00000030382.
    GeneIDi26459.
    KEGGimmu:26459.
    UCSCiuc009fey.3. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ223959 mRNA. Translation: CAA11688.1 .
    BC013335 mRNA. Translation: AAH13335.1 .
    BC013272 mRNA. Translation: AAH13272.1 .
    BC145823 mRNA. Translation: AAI45824.1 .
    BC145825 mRNA. Translation: AAI45826.1 .
    AF072760 mRNA. Translation: AAC40189.1 .
    CCDSi CCDS20821.1.
    PIRi JW0107.
    RefSeqi NP_033538.2. NM_009512.2.
    UniGenei Mm.10984.

    3D structure databases

    ProteinModelPortali Q4LDG0.
    SMRi Q4LDG0. Positions 111-660.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q4LDG0. 2 interactions.
    MINTi MINT-1863806.

    Protein family/group databases

    TCDBi 4.C.1.1.8. the proposed fatty acid transporter (fat) family.

    Proteomic databases

    MaxQBi Q4LDG0.
    PaxDbi Q4LDG0.
    PRIDEi Q4LDG0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000032539 ; ENSMUSP00000032539 ; ENSMUSG00000030382 .
    GeneIDi 26459.
    KEGGi mmu:26459.
    UCSCi uc009fey.3. mouse.

    Organism-specific databases

    CTDi 10998.
    MGIi MGI:1347100. Slc27a5.

    Phylogenomic databases

    eggNOGi COG0318.
    GeneTreei ENSGT00550000074420.
    HOGENOMi HOG000044189.
    HOVERGENi HBG005642.
    InParanoidi A6H6C1.
    KOi K08748.
    OMAi PHWLSLA.
    OrthoDBi EOG7W6WKB.
    PhylomeDBi Q4LDG0.
    TreeFami TF313430.

    Enzyme and pathway databases

    Reactomei REACT_203193. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
    REACT_210229. Synthesis of bile acids and bile salts via 24-hydroxycholesterol.

    Miscellaneous databases

    ChiTaRSi SLC27A5. mouse.
    NextBioi 304573.
    PROi Q4LDG0.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q4LDG0.
    Genevestigatori Q4LDG0.

    Family and domain databases

    InterProi IPR025110. AMP-bd_C.
    IPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    [Graphical view ]
    Pfami PF00501. AMP-binding. 1 hit.
    PF13193. AMP-binding_C. 1 hit.
    [Graphical view ]
    PROSITEi PS00455. AMP_BINDING. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A novel relative of the very-long-chain acyl-CoA synthetase and fatty acid transporter protein genes with a distinct expression pattern."
      Berger J., Truppe C., Neumann H., Forss-Petter S.
      Biochem. Biophys. Res. Commun. 247:255-260(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
      Tissue: Liver.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Brain and Salivary gland.
    3. "A family of fatty acid transporters conserved from mycobacterium to man."
      Hirsch D., Stahl A., Lodish H.F.
      Proc. Natl. Acad. Sci. U.S.A. 95:8625-8629(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 28-689.
    4. "Participation of two members of the very long-chain acyl-CoA synthetase family in bile acid synthesis and recycling."
      Mihalik S.J., Steinberg S.J., Pei Z., Park J., Kim do G., Heinzer A.K., Dacremont G., Wanders R.J., Cuebas D.A., Smith K.D., Watkins P.A.
      J. Biol. Chem. 277:24771-24779(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiS27A5_MOUSE
    AccessioniPrimary (citable) accession number: Q4LDG0
    Secondary accession number(s): A6H6C1, O88694, Q91VD5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 22, 2005
    Last sequence update: November 22, 2005
    Last modified: October 1, 2014
    This is version 86 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3