Bile acyl-CoA synthetase - Mus musculus (Mouse)

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Q4LDG0 (S27A5_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 62. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Bile acyl-CoA synthetase
Short name=BACS
EC=6.2.1.7

Alternative name(s):
Bile acid-CoA ligase
Short name=BA-CoA ligase
Short name=BAL
Cholate--CoA ligase
Fatty acid transport protein 5
Short name=FATP-5
Solute carrier family 27 member 5
Very long-chain acyl-CoA synthetase-related protein
Short name=VLACS-related
Short name=VLACSR

Gene names

Name:	Slc27a5
Synonyms:	Acsb, Acsvl6, Fatp5, Vlacsr

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	689 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Acyl-CoA synthetase involved in bile acid metabolism. Proposed to catalyze the first step in the conjugation of C24 bile acids (choloneates) to glycine and taurine before excretion into bile canaliculi by activating them to their CoA thioesters. Seems to activate secondary bile acids entering the liver from the enterohepatic circulation By similarity.
Catalytic activity	ATP + cholate + CoA = AMP + diphosphate + choloyl-CoA. ATP + (25R)-3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestan-26-oate + CoA = AMP + diphosphate + (25R)-3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestanoyl-CoA.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Tissue specificity	In liver expressed in a periportal distribution. Ref.4
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	bile acid metabolic process Inferred from mutant phenotype. Source: MGI ketone body biosynthetic process Inferred from mutant phenotype. Source: MGI long-chain fatty acid metabolic process Traceable author statement. Source: MGI plasma membrane long-chain fatty acid transport Inferred from mutant phenotype. Source: MGI triglyceride mobilization Inferred from mutant phenotype. Source: MGI
Cellular component	basal plasma membrane Inferred from direct assay. Source: MGI endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cholate-CoA ligase activity Inferred from electronic annotation. Source: EC fatty acid transporter activity Inferred from direct assay Ref.3. Source: MGI long-chain fatty acid-CoA ligase activity Traceable author statement. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 689

689

Bile acyl-CoA synthetase

PRO_0000193214

Regions

Topological domain

1 – 29

Cytoplasmic By similarity

Transmembrane

30 – 50

Helical; Potential

Transmembrane

55 – 75

Helical; Potential

Topological domain

76 – 689

614

Cytoplasmic By similarity

Nucleotide binding

292 – 303

AMP Potential

Experimental info

Sequence conflict

K → I in CAA11688. Ref.1

Sequence conflict

408

N → T in AAC40189. Ref.3

Sequence conflict

568

C → S in CAA11688. Ref.1

Sequence conflict

688

N → K in CAA11688. Ref.1

Sequences

Sequence

Length

Mass (Da)

Tools

Q4LDG0 [UniParc].

Last modified November 22, 2005. Version 2.
Checksum: 1642BBC2CF04FAA3
Show »

FASTA

689

76,203

        10         20         30         40         50         60 
MGIWKKLTLL LLLLLLVGLG QPPWPAAMAL ALRWFLGDPT CLVLLGLALL GRPWISSWMP 

        70         80         90        100        110        120 
HWLSLVGAAL TLFLLPLQPP PGLRWLHKDV AFTFKMLFYG LKFRRRLNKH PPETFVDALE 

       130        140        150        160        170        180 
RQALAWPDRV ALVCTGSEGS SITNSQLDAR SCQAAWVLKA KLKDAVIQNT RDAAAILVLP 

       190        200        210        220        230        240 
SKTISALSVF LGLAKLGCPV AWINPHSRGM PLLHSVRSSG ASVLIVDPDL QENLEEVLPK 

       250        260        270        280        290        300 
LLAENIHCFY LGHSSPTPGV EALGASLDAA PSDPVPASLR ATIKWKSPAI FIFTSGTTGL 

       310        320        330        340        350        360 
PKPAILSHER VIQVSNVLSF CGCRADDVVY DVLPLYHTIG LVLGFLGCLQ VGATCVLAPK 

       370        380        390        400        410        420 
FSASRFWAEC RQHGVTVILY VGEILRYLCN VPEQPEDKIH TVRLAMGNGL RANVWKNFQQ 

       430        440        450        460        470        480 
RFGPIRIWEF YGSTEGNVGL MNYVGHCGAV GRTSCILRML TPFELVQFDI ETAEPLRDKQ 

       490        500        510        520        530        540 
GFCIPVEPGK PGLLLTKVRK NQPFLGYRGS QAESNRKLVA NVRRVGDLYF NTGDVLTLDQ 

       550        560        570        580        590        600 
EGFFYFQDRL GDTFRWKGEN VSTGEVECVL SSLDFLEEVN VYGVPVPGCE GKVGMAAVKL 

       610        620        630        640        650        660 
APGKTFDGQK LYQHVRSWLP AYATPHFIRI QDSLEITNTY KLVKSRLVRE GFDVGIIADP 

       670        680 
LYILDNKAQT FRSLMPDVYQ AVCEGTWNL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel relative of the very-long-chain acyl-CoA synthetase and fatty acid transporter protein genes with a distinct expression pattern." Berger J., Truppe C., Neumann H., Forss-Petter S. Biochem. Biophys. Res. Commun. 247:255-260(1998) [PubMed: 9642112] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Liver.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Brain and Salivary gland.
[3]	"A family of fatty acid transporters conserved from mycobacterium to man." Hirsch D., Stahl A., Lodish H.F. Proc. Natl. Acad. Sci. U.S.A. 95:8625-8629(1998) [PubMed: 9671728] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 28-689.
[4]	"Participation of two members of the very long-chain acyl-CoA synthetase family in bile acid synthesis and recycling." Mihalik S.J., Steinberg S.J., Pei Z., Park J., Kim do G., Heinzer A.K., Dacremont G., Wanders R.J., Cuebas D.A., Smith K.D., Watkins P.A. J. Biol. Chem. 277:24771-24779(2002) [PubMed: 11980911] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ223959 mRNA. Translation: CAA11688.1. BC013335 mRNA. Translation: AAH13335.1. BC013272 mRNA. Translation: AAH13272.1. BC145823 mRNA. Translation: AAI45824.1. BC145825 mRNA. Translation: AAI45826.1. AF072760 mRNA. Translation: AAC40189.1.
IPI	IPI00313236.
PIR	JW0107.
RefSeq	NP_033538.2. NM_009512.2.
UniGene	Mm.10984.
3D structure databases
ProteinModelPortal	Q4LDG0.
ModBase	Search...
Protein-protein interaction databases
STRING	Q4LDG0.
Protein family/group databases
TCDB	4.C.1.1.8. proposed fatty acid transporter (FAT) family.
Proteomic databases
PRIDE	Q4LDG0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000032539; ENSMUSP00000032539; ENSMUSG00000030382.
GeneID	26459.
KEGG	mmu:26459.
UCSC	uc009fey.2. mouse.
Organism-specific databases
CTD	10998.
MGI	MGI:1347100. Slc27a5.
Phylogenomic databases
eggNOG	roNOG09217.
GeneTree	ENSGT00550000074420.
HOGENOM	HBG712034.
HOVERGEN	HBG005642.
InParanoid	Q4LDG0.
OMA	TFVDAFE.
OrthoDB	EOG41ZF9B.
PhylomeDB	Q4LDG0.
Gene expression databases
ArrayExpress	Q4LDG0.
Bgee	Q4LDG0.
Genevestigator	Q4LDG0.
GermOnline	ENSMUSG00000030382. Mus musculus.
Family and domain databases
InterPro	IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
KO	K08748.
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	304573.
SOURCE	Search...

Entry information

Entry name

S27A5_MOUSE

Accession

Primary (citable) accession number: Q4LDG0
Secondary accession number(s): A6H6C1, O88694, Q91VD5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	November 22, 2005
Last modified:	November 16, 2011
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents