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Protein

Bile acyl-CoA synthetase

Gene

Slc27a5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acyl-CoA synthetase involved in bile acid metabolism. Proposed to catalyze the first step in the conjugation of C24 bile acids (choloneates) to glycine and taurine before excretion into bile canaliculi by activating them to their CoA thioesters. Seems to activate secondary bile acids entering the liver from the enterohepatic circulation (By similarity).By similarity

Catalytic activityi

ATP + cholate + CoA = AMP + diphosphate + choloyl-CoA.
ATP + (25R)-3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestan-26-oate + CoA = AMP + diphosphate + (25R)-3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestanoyl-CoA.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi292 – 303AMPSequence analysisAdd BLAST12

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • cholate-CoA ligase activity Source: UniProtKB-EC
  • fatty acid transporter activity Source: MGI
  • long-chain fatty acid-CoA ligase activity Source: MGI
  • very long-chain fatty acid-CoA ligase activity Source: MGI

GO - Biological processi

  • bile acid biosynthetic process Source: MGI
  • bile acid metabolic process Source: MGI
  • fatty acid transport Source: MGI
  • ketone body biosynthetic process Source: MGI
  • long-chain fatty acid metabolic process Source: MGI
  • plasma membrane long-chain fatty acid transport Source: MGI
  • triglyceride mobilization Source: MGI
  • very long-chain fatty acid metabolic process Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-159418. Recycling of bile acids and salts.
R-MMU-193368. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
R-MMU-193775. Synthesis of bile acids and bile salts via 24-hydroxycholesterol.

Protein family/group databases

TCDBi4.C.1.1.8. the proposed fatty acid transporter (fat) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Bile acyl-CoA synthetase (EC:6.2.1.7)
Short name:
BACS
Alternative name(s):
Bile acid-CoA ligase
Short name:
BA-CoA ligase
Short name:
BAL
Cholate--CoA ligase
Fatty acid transport protein 5
Short name:
FATP-5
Solute carrier family 27 member 5
Very long-chain acyl-CoA synthetase-related protein
Short name:
VLACS-related
Short name:
VLACSR
Gene namesi
Name:Slc27a5
Synonyms:Acsb, Acsvl6, Fatp5, Vlacsr
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1347100. Slc27a5.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 29CytoplasmicBy similarityAdd BLAST29
Transmembranei30 – 50HelicalSequence analysisAdd BLAST21
Transmembranei55 – 75HelicalSequence analysisAdd BLAST21
Topological domaini76 – 689CytoplasmicBy similarityAdd BLAST614

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001932141 – 689Bile acyl-CoA synthetaseAdd BLAST689

Proteomic databases

MaxQBiQ4LDG0.
PaxDbiQ4LDG0.
PeptideAtlasiQ4LDG0.
PRIDEiQ4LDG0.

PTM databases

iPTMnetiQ4LDG0.
PhosphoSitePlusiQ4LDG0.

Expressioni

Tissue specificityi

In liver expressed in a periportal distribution.1 Publication

Gene expression databases

BgeeiENSMUSG00000030382.
ExpressionAtlasiQ4LDG0. baseline and differential.
GenevisibleiQ4LDG0. MM.

Interactioni

Protein-protein interaction databases

IntActiQ4LDG0. 2 interactors.
MINTiMINT-1863806.
STRINGi10090.ENSMUSP00000032539.

Structurei

3D structure databases

ProteinModelPortaliQ4LDG0.
SMRiQ4LDG0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1179. Eukaryota.
ENOG410XQ8T. LUCA.
GeneTreeiENSGT00550000074420.
HOGENOMiHOG000044189.
HOVERGENiHBG005642.
InParanoidiQ4LDG0.
KOiK08748.
OMAiCFWDDCR.
OrthoDBiEOG091G0B76.
PhylomeDBiQ4LDG0.
TreeFamiTF313430.

Family and domain databases

InterProiIPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
IPR030302. FATP5.
[Graphical view]
PANTHERiPTHR24096:SF99. PTHR24096:SF99. 1 hit.
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4LDG0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGIWKKLTLL LLLLLLVGLG QPPWPAAMAL ALRWFLGDPT CLVLLGLALL
60 70 80 90 100
GRPWISSWMP HWLSLVGAAL TLFLLPLQPP PGLRWLHKDV AFTFKMLFYG
110 120 130 140 150
LKFRRRLNKH PPETFVDALE RQALAWPDRV ALVCTGSEGS SITNSQLDAR
160 170 180 190 200
SCQAAWVLKA KLKDAVIQNT RDAAAILVLP SKTISALSVF LGLAKLGCPV
210 220 230 240 250
AWINPHSRGM PLLHSVRSSG ASVLIVDPDL QENLEEVLPK LLAENIHCFY
260 270 280 290 300
LGHSSPTPGV EALGASLDAA PSDPVPASLR ATIKWKSPAI FIFTSGTTGL
310 320 330 340 350
PKPAILSHER VIQVSNVLSF CGCRADDVVY DVLPLYHTIG LVLGFLGCLQ
360 370 380 390 400
VGATCVLAPK FSASRFWAEC RQHGVTVILY VGEILRYLCN VPEQPEDKIH
410 420 430 440 450
TVRLAMGNGL RANVWKNFQQ RFGPIRIWEF YGSTEGNVGL MNYVGHCGAV
460 470 480 490 500
GRTSCILRML TPFELVQFDI ETAEPLRDKQ GFCIPVEPGK PGLLLTKVRK
510 520 530 540 550
NQPFLGYRGS QAESNRKLVA NVRRVGDLYF NTGDVLTLDQ EGFFYFQDRL
560 570 580 590 600
GDTFRWKGEN VSTGEVECVL SSLDFLEEVN VYGVPVPGCE GKVGMAAVKL
610 620 630 640 650
APGKTFDGQK LYQHVRSWLP AYATPHFIRI QDSLEITNTY KLVKSRLVRE
660 670 680
GFDVGIIADP LYILDNKAQT FRSLMPDVYQ AVCEGTWNL
Length:689
Mass (Da):76,203
Last modified:November 22, 2005 - v2
Checksum:i1642BBC2CF04FAA3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti88K → I in CAA11688 (PubMed:9642112).Curated1
Sequence conflicti408N → T in AAC40189 (PubMed:9671728).Curated1
Sequence conflicti568C → S in CAA11688 (PubMed:9642112).Curated1
Sequence conflicti688N → K in CAA11688 (PubMed:9642112).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ223959 mRNA. Translation: CAA11688.1.
BC013335 mRNA. Translation: AAH13335.1.
BC013272 mRNA. Translation: AAH13272.1.
BC145823 mRNA. Translation: AAI45824.1.
BC145825 mRNA. Translation: AAI45826.1.
AF072760 mRNA. Translation: AAC40189.1.
CCDSiCCDS20821.1.
PIRiJW0107.
RefSeqiNP_033538.2. NM_009512.2.
UniGeneiMm.10984.

Genome annotation databases

EnsembliENSMUST00000032539; ENSMUSP00000032539; ENSMUSG00000030382.
GeneIDi26459.
KEGGimmu:26459.
UCSCiuc009fey.3. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ223959 mRNA. Translation: CAA11688.1.
BC013335 mRNA. Translation: AAH13335.1.
BC013272 mRNA. Translation: AAH13272.1.
BC145823 mRNA. Translation: AAI45824.1.
BC145825 mRNA. Translation: AAI45826.1.
AF072760 mRNA. Translation: AAC40189.1.
CCDSiCCDS20821.1.
PIRiJW0107.
RefSeqiNP_033538.2. NM_009512.2.
UniGeneiMm.10984.

3D structure databases

ProteinModelPortaliQ4LDG0.
SMRiQ4LDG0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ4LDG0. 2 interactors.
MINTiMINT-1863806.
STRINGi10090.ENSMUSP00000032539.

Protein family/group databases

TCDBi4.C.1.1.8. the proposed fatty acid transporter (fat) family.

PTM databases

iPTMnetiQ4LDG0.
PhosphoSitePlusiQ4LDG0.

Proteomic databases

MaxQBiQ4LDG0.
PaxDbiQ4LDG0.
PeptideAtlasiQ4LDG0.
PRIDEiQ4LDG0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000032539; ENSMUSP00000032539; ENSMUSG00000030382.
GeneIDi26459.
KEGGimmu:26459.
UCSCiuc009fey.3. mouse.

Organism-specific databases

CTDi10998.
MGIiMGI:1347100. Slc27a5.

Phylogenomic databases

eggNOGiKOG1179. Eukaryota.
ENOG410XQ8T. LUCA.
GeneTreeiENSGT00550000074420.
HOGENOMiHOG000044189.
HOVERGENiHBG005642.
InParanoidiQ4LDG0.
KOiK08748.
OMAiCFWDDCR.
OrthoDBiEOG091G0B76.
PhylomeDBiQ4LDG0.
TreeFamiTF313430.

Enzyme and pathway databases

ReactomeiR-MMU-159418. Recycling of bile acids and salts.
R-MMU-193368. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
R-MMU-193775. Synthesis of bile acids and bile salts via 24-hydroxycholesterol.

Miscellaneous databases

PROiQ4LDG0.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030382.
ExpressionAtlasiQ4LDG0. baseline and differential.
GenevisibleiQ4LDG0. MM.

Family and domain databases

InterProiIPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
IPR030302. FATP5.
[Graphical view]
PANTHERiPTHR24096:SF99. PTHR24096:SF99. 1 hit.
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiS27A5_MOUSE
AccessioniPrimary (citable) accession number: Q4LDG0
Secondary accession number(s): A6H6C1, O88694, Q91VD5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 22, 2005
Last modified: November 2, 2016
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.