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Q4LDG0

- S27A5_MOUSE

UniProt

Q4LDG0 - S27A5_MOUSE

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Protein

Bile acyl-CoA synthetase

Gene

Slc27a5

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Acyl-CoA synthetase involved in bile acid metabolism. Proposed to catalyze the first step in the conjugation of C24 bile acids (choloneates) to glycine and taurine before excretion into bile canaliculi by activating them to their CoA thioesters. Seems to activate secondary bile acids entering the liver from the enterohepatic circulation (By similarity).By similarity

Catalytic activityi

ATP + cholate + CoA = AMP + diphosphate + choloyl-CoA.
ATP + (25R)-3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestan-26-oate + CoA = AMP + diphosphate + (25R)-3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestanoyl-CoA.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi292 – 30312AMPSequence AnalysisAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cholate-CoA ligase activity Source: UniProtKB-EC
  3. fatty acid transporter activity Source: MGI
  4. long-chain fatty acid-CoA ligase activity Source: MGI
  5. very long-chain fatty acid-CoA ligase activity Source: Ensembl

GO - Biological processi

  1. bile acid biosynthetic process Source: Ensembl
  2. bile acid metabolic process Source: MGI
  3. fatty acid transport Source: MGI
  4. ketone body biosynthetic process Source: MGI
  5. long-chain fatty acid metabolic process Source: MGI
  6. plasma membrane long-chain fatty acid transport Source: MGI
  7. triglyceride mobilization Source: MGI
  8. very long-chain fatty acid metabolic process Source: Ensembl
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_203193. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
REACT_210229. Synthesis of bile acids and bile salts via 24-hydroxycholesterol.
REACT_239346. Recycling of bile acids and salts.

Protein family/group databases

TCDBi4.C.1.1.8. the proposed fatty acid transporter (fat) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Bile acyl-CoA synthetase (EC:6.2.1.7)
Short name:
BACS
Alternative name(s):
Bile acid-CoA ligase
Short name:
BA-CoA ligase
Short name:
BAL
Cholate--CoA ligase
Fatty acid transport protein 5
Short name:
FATP-5
Solute carrier family 27 member 5
Very long-chain acyl-CoA synthetase-related protein
Short name:
VLACS-related
Short name:
VLACSR
Gene namesi
Name:Slc27a5
Synonyms:Acsb, Acsvl6, Fatp5, Vlacsr
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 7

Organism-specific databases

MGIiMGI:1347100. Slc27a5.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2929CytoplasmicBy similarityAdd
BLAST
Transmembranei30 – 5021HelicalSequence AnalysisAdd
BLAST
Transmembranei55 – 7521HelicalSequence AnalysisAdd
BLAST
Topological domaini76 – 689614CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  1. basal plasma membrane Source: MGI
  2. endoplasmic reticulum Source: MGI
  3. integral component of endoplasmic reticulum membrane Source: Ensembl
  4. protein complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 689689Bile acyl-CoA synthetasePRO_0000193214Add
BLAST

Proteomic databases

MaxQBiQ4LDG0.
PaxDbiQ4LDG0.
PRIDEiQ4LDG0.

Expressioni

Tissue specificityi

In liver expressed in a periportal distribution.1 Publication

Gene expression databases

BgeeiQ4LDG0.
ExpressionAtlasiQ4LDG0. baseline and differential.
GenevestigatoriQ4LDG0.

Interactioni

Protein-protein interaction databases

IntActiQ4LDG0. 2 interactions.
MINTiMINT-1863806.

Structurei

3D structure databases

ProteinModelPortaliQ4LDG0.
SMRiQ4LDG0. Positions 111-660.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0318.
GeneTreeiENSGT00550000074420.
HOGENOMiHOG000044189.
HOVERGENiHBG005642.
InParanoidiQ4LDG0.
KOiK08748.
OMAiPHWLSLA.
OrthoDBiEOG7W6WKB.
PhylomeDBiQ4LDG0.
TreeFamiTF313430.

Family and domain databases

InterProiIPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4LDG0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGIWKKLTLL LLLLLLVGLG QPPWPAAMAL ALRWFLGDPT CLVLLGLALL
60 70 80 90 100
GRPWISSWMP HWLSLVGAAL TLFLLPLQPP PGLRWLHKDV AFTFKMLFYG
110 120 130 140 150
LKFRRRLNKH PPETFVDALE RQALAWPDRV ALVCTGSEGS SITNSQLDAR
160 170 180 190 200
SCQAAWVLKA KLKDAVIQNT RDAAAILVLP SKTISALSVF LGLAKLGCPV
210 220 230 240 250
AWINPHSRGM PLLHSVRSSG ASVLIVDPDL QENLEEVLPK LLAENIHCFY
260 270 280 290 300
LGHSSPTPGV EALGASLDAA PSDPVPASLR ATIKWKSPAI FIFTSGTTGL
310 320 330 340 350
PKPAILSHER VIQVSNVLSF CGCRADDVVY DVLPLYHTIG LVLGFLGCLQ
360 370 380 390 400
VGATCVLAPK FSASRFWAEC RQHGVTVILY VGEILRYLCN VPEQPEDKIH
410 420 430 440 450
TVRLAMGNGL RANVWKNFQQ RFGPIRIWEF YGSTEGNVGL MNYVGHCGAV
460 470 480 490 500
GRTSCILRML TPFELVQFDI ETAEPLRDKQ GFCIPVEPGK PGLLLTKVRK
510 520 530 540 550
NQPFLGYRGS QAESNRKLVA NVRRVGDLYF NTGDVLTLDQ EGFFYFQDRL
560 570 580 590 600
GDTFRWKGEN VSTGEVECVL SSLDFLEEVN VYGVPVPGCE GKVGMAAVKL
610 620 630 640 650
APGKTFDGQK LYQHVRSWLP AYATPHFIRI QDSLEITNTY KLVKSRLVRE
660 670 680
GFDVGIIADP LYILDNKAQT FRSLMPDVYQ AVCEGTWNL
Length:689
Mass (Da):76,203
Last modified:November 22, 2005 - v2
Checksum:i1642BBC2CF04FAA3
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti88 – 881K → I in CAA11688. (PubMed:9642112)Curated
Sequence conflicti408 – 4081N → T in AAC40189. (PubMed:9671728)Curated
Sequence conflicti568 – 5681C → S in CAA11688. (PubMed:9642112)Curated
Sequence conflicti688 – 6881N → K in CAA11688. (PubMed:9642112)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ223959 mRNA. Translation: CAA11688.1.
BC013335 mRNA. Translation: AAH13335.1.
BC013272 mRNA. Translation: AAH13272.1.
BC145823 mRNA. Translation: AAI45824.1.
BC145825 mRNA. Translation: AAI45826.1.
AF072760 mRNA. Translation: AAC40189.1.
CCDSiCCDS20821.1.
PIRiJW0107.
RefSeqiNP_033538.2. NM_009512.2.
UniGeneiMm.10984.

Genome annotation databases

EnsembliENSMUST00000032539; ENSMUSP00000032539; ENSMUSG00000030382.
GeneIDi26459.
KEGGimmu:26459.
UCSCiuc009fey.3. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ223959 mRNA. Translation: CAA11688.1 .
BC013335 mRNA. Translation: AAH13335.1 .
BC013272 mRNA. Translation: AAH13272.1 .
BC145823 mRNA. Translation: AAI45824.1 .
BC145825 mRNA. Translation: AAI45826.1 .
AF072760 mRNA. Translation: AAC40189.1 .
CCDSi CCDS20821.1.
PIRi JW0107.
RefSeqi NP_033538.2. NM_009512.2.
UniGenei Mm.10984.

3D structure databases

ProteinModelPortali Q4LDG0.
SMRi Q4LDG0. Positions 111-660.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q4LDG0. 2 interactions.
MINTi MINT-1863806.

Protein family/group databases

TCDBi 4.C.1.1.8. the proposed fatty acid transporter (fat) family.

Proteomic databases

MaxQBi Q4LDG0.
PaxDbi Q4LDG0.
PRIDEi Q4LDG0.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000032539 ; ENSMUSP00000032539 ; ENSMUSG00000030382 .
GeneIDi 26459.
KEGGi mmu:26459.
UCSCi uc009fey.3. mouse.

Organism-specific databases

CTDi 10998.
MGIi MGI:1347100. Slc27a5.

Phylogenomic databases

eggNOGi COG0318.
GeneTreei ENSGT00550000074420.
HOGENOMi HOG000044189.
HOVERGENi HBG005642.
InParanoidi Q4LDG0.
KOi K08748.
OMAi PHWLSLA.
OrthoDBi EOG7W6WKB.
PhylomeDBi Q4LDG0.
TreeFami TF313430.

Enzyme and pathway databases

Reactomei REACT_203193. Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol.
REACT_210229. Synthesis of bile acids and bile salts via 24-hydroxycholesterol.
REACT_239346. Recycling of bile acids and salts.

Miscellaneous databases

NextBioi 304573.
PROi Q4LDG0.
SOURCEi Search...

Gene expression databases

Bgeei Q4LDG0.
ExpressionAtlasi Q4LDG0. baseline and differential.
Genevestigatori Q4LDG0.

Family and domain databases

InterProi IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view ]
Pfami PF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view ]
PROSITEi PS00455. AMP_BINDING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A novel relative of the very-long-chain acyl-CoA synthetase and fatty acid transporter protein genes with a distinct expression pattern."
    Berger J., Truppe C., Neumann H., Forss-Petter S.
    Biochem. Biophys. Res. Commun. 247:255-260(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
    Tissue: Liver.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Brain and Salivary gland.
  3. "A family of fatty acid transporters conserved from mycobacterium to man."
    Hirsch D., Stahl A., Lodish H.F.
    Proc. Natl. Acad. Sci. U.S.A. 95:8625-8629(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 28-689.
  4. "Participation of two members of the very long-chain acyl-CoA synthetase family in bile acid synthesis and recycling."
    Mihalik S.J., Steinberg S.J., Pei Z., Park J., Kim do G., Heinzer A.K., Dacremont G., Wanders R.J., Cuebas D.A., Smith K.D., Watkins P.A.
    J. Biol. Chem. 277:24771-24779(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiS27A5_MOUSE
AccessioniPrimary (citable) accession number: Q4LDG0
Secondary accession number(s): A6H6C1, O88694, Q91VD5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 22, 2005
Last modified: November 26, 2014
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3