Reviewed, UniProtKB/Swiss-Prot Q4LDG0 (S27A5_MOUSE)

Names and origin

Protein names

Recommended name:
    Bile acyl-CoA synthetase
      Short name=BACS
    EC=6.2.1.7
Alternative name(s):
    Bile acid-CoA ligase
      Short name=BA-CoA ligase
      Short name=BAL
    Cholate--CoA ligase
    Very long-chain acyl-CoA synthetase-related protein
      Short name=VLACS-related
      Short name=VLACSR
    Fatty acid transport protein 5
      Short name=FATP-5
    Solute carrier family 27 member 5

Gene names

Name:	Slc27a5
Synonyms:	Acsb, Acsvl6, Fatp5, Vlacsr

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	689 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Acyl-CoA synthetase involved in bile acid metabolism. Proposed to catalyze the first step in the conjugation of C24 bile acids (choloneates) to glycine and taurine before excretion into bile canaliculi by activating them to their CoA thioesters. Seems to activate secondary bile acids entering the liver from the enterohepatic circulation By similarity.
Catalytic activity	ATP + cholate + CoA = AMP + diphosphate + choloyl-CoA. ATP + (25R)-3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestan-26-oate + CoA = AMP + diphosphate + (25R)-3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestanoyl-CoA.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Tissue specificity	In liver expressed in a periportal distribution. Ref.4
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Biological process	Fatty acid metabolism Lipid metabolism
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	long-chain fatty acid metabolic process Traceable author statement. Source: MGI
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cholate-CoA ligase activity Inferred from electronic annotation. Source: EC long-chain-fatty-acid-CoA ligase activity Traceable author statement. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 689	689	Bile acyl-CoA synthetase		PRO_0000193214
Regions
	Topological domain	1 – 7	7	Cytoplasmic By similarity
	Transmembrane	8 – 28	21	Potential
	Transmembrane	30 – 50	21	Potential
	Transmembrane	55 – 75	21	Potential
	Topological domain	76 – 689	614	Cytoplasmic By similarity
	Nucleotide binding	292 – 303	12	AMP Potential
Experimental info
	Sequence conflict	88	1	K → I in CAA11688. Ref.1
	Sequence conflict	408	1	N → T in AAC40189. Ref.3
	Sequence conflict	568	1	C → S in CAA11688. Ref.1
	Sequence conflict	688	1	N → K in CAA11688. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q4LDG0-1 [UniParc]. Last modified November 22, 2005. Version 2. Checksum: 1642BBC2CF04FAA3	FASTA	689	76,203
	10 20 30 40 50 60 MGIWKKLTLL LLLLLLVGLG QPPWPAAMAL ALRWFLGDPT CLVLLGLALL GRPWISSWMP 70 80 90 100 110 120 HWLSLVGAAL TLFLLPLQPP PGLRWLHKDV AFTFKMLFYG LKFRRRLNKH PPETFVDALE 130 140 150 160 170 180 RQALAWPDRV ALVCTGSEGS SITNSQLDAR SCQAAWVLKA KLKDAVIQNT RDAAAILVLP 190 200 210 220 230 240 SKTISALSVF LGLAKLGCPV AWINPHSRGM PLLHSVRSSG ASVLIVDPDL QENLEEVLPK 250 260 270 280 290 300 LLAENIHCFY LGHSSPTPGV EALGASLDAA PSDPVPASLR ATIKWKSPAI FIFTSGTTGL 310 320 330 340 350 360 PKPAILSHER VIQVSNVLSF CGCRADDVVY DVLPLYHTIG LVLGFLGCLQ VGATCVLAPK 370 380 390 400 410 420 FSASRFWAEC RQHGVTVILY VGEILRYLCN VPEQPEDKIH TVRLAMGNGL RANVWKNFQQ 430 440 450 460 470 480 RFGPIRIWEF YGSTEGNVGL MNYVGHCGAV GRTSCILRML TPFELVQFDI ETAEPLRDKQ 490 500 510 520 530 540 GFCIPVEPGK PGLLLTKVRK NQPFLGYRGS QAESNRKLVA NVRRVGDLYF NTGDVLTLDQ 550 560 570 580 590 600 EGFFYFQDRL GDTFRWKGEN VSTGEVECVL SSLDFLEEVN VYGVPVPGCE GKVGMAAVKL 610 620 630 640 650 660 APGKTFDGQK LYQHVRSWLP AYATPHFIRI QDSLEITNTY KLVKSRLVRE GFDVGIIADP 670 680 LYILDNKAQT FRSLMPDVYQ AVCEGTWNL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel relative of the very-long-chain acyl-CoA synthetase and fatty acid transporter protein genes with a distinct expression pattern." Berger J., Truppe C., Neumann H., Forss-Petter S. Biochem. Biophys. Res. Commun. 247:255-260(1998) [PubMed: 9642112] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Liver.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Salivary gland.
[3]	"A family of fatty acid transporters conserved from mycobacterium to man." Hirsch D., Stahl A., Lodish H.F. Proc. Natl. Acad. Sci. U.S.A. 95:8625-8629(1998) [PubMed: 9671728] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 28-689.
[4]	"Participation of two members of the very long-chain acyl-CoA synthetase family in bile acid synthesis and recycling." Mihalik S.J., Steinberg S.J., Pei Z., Park J., Kim do G., Heinzer A.K., Dacremont G., Wanders R.J., Cuebas D.A., Smith K.D., Watkins P.A. J. Biol. Chem. 277:24771-24779(2002) [PubMed: 11980911] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ223959 mRNA. Translation: CAA11688.1. BC013335 mRNA. Translation: AAH13335.1. BC013272 mRNA. Translation: AAH13272.1. AF072760 mRNA. Translation: AAC40189.1.
IPI	IPI00313236.
PIR	JW0107.
RefSeq	NP_033538.1.
UniGene	Mm.10984
3D structure databases
ModBase	Search...
Protein family/group databases
TCDB	4.C.1.1.8. proposed fatty acid transporter (FAT) family.
Genome annotation databases
Ensembl	ENSMUSG00000030382. Mus musculus. [Contig view]
GeneID	26459.
KEGG	mmu:26459.
Organism-specific databases
MGI	MGI:1347100. Slc27a5.
Phylogenomic databases
HOGENOM	Q4LDG0.
HOVERGEN	Q4LDG0.
OMA	Q4LDG0. TFVDAFE.
Enzyme and pathway databases
BRENDA	6.2.1.7. 244.
Gene expression databases
ArrayExpress	Q4LDG0.
Bgee	Q4LDG0.
GermOnline	ENSMUSG00000030382. Mus musculus.
Family and domain databases
InterPro	IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	304573.
SOURCE	Search...

Entry information

Entry name

S27A5_MOUSE

Accession

Primary (citable) accession number: Q4LDG0
Secondary accession number(s): O88694, Q91VD5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	November 22, 2005
Last modified:	June 16, 2009
This is version 39 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)