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Protein

Bile acyl-CoA synthetase

Gene

Slc27a5

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acyl-CoA synthetase involved in bile acid metabolism. Proposed to catalyze the first step in the conjugation of C24 bile acids (choloneates) to glycine and taurine before excretion into bile canaliculi by activating them to their CoA thioesters. Seems to activate secondary bile acids entering the liver from the enterohepatic circulation (By similarity).By similarity

Catalytic activityi

ATP + cholate + CoA = AMP + diphosphate + choloyl-CoA.
ATP + (25R)-3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestan-26-oate + CoA = AMP + diphosphate + (25R)-3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholestanoyl-CoA.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi292 – 303AMPSequence analysisAdd BLAST12

GO - Molecular functioni

GO - Biological processi

  • bile acid biosynthetic process Source: MGI
  • bile acid metabolic process Source: MGI
  • fatty acid transport Source: MGI
  • ketone body biosynthetic process Source: MGI
  • long-chain fatty acid metabolic process Source: MGI
  • plasma membrane long-chain fatty acid transport Source: MGI
  • triglyceride mobilization Source: MGI
  • very long-chain fatty acid metabolic process Source: MGI

Keywordsi

Molecular functionLigase
Biological processFatty acid metabolism, Lipid metabolism
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-159418 Recycling of bile acids and salts
R-MMU-193368 Synthesis of bile acids and bile salts via 7alpha-hydroxycholesterol
R-MMU-193775 Synthesis of bile acids and bile salts via 24-hydroxycholesterol

Protein family/group databases

TCDBi4.C.1.1.8 the fatty acid transporter (fat) family

Names & Taxonomyi

Protein namesi
Recommended name:
Bile acyl-CoA synthetase (EC:6.2.1.7)
Short name:
BACS
Alternative name(s):
Bile acid-CoA ligase
Short name:
BA-CoA ligase
Short name:
BAL
Cholate--CoA ligase
Fatty acid transport protein 5
Short name:
FATP-5
Solute carrier family 27 member 5
Very long-chain acyl-CoA synthetase-related protein
Short name:
VLACS-related
Short name:
VLACSR
Gene namesi
Name:Slc27a5
Synonyms:Acsb, Acsvl6, Fatp5, Vlacsr
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1347100 Slc27a5

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 29CytoplasmicBy similarityAdd BLAST29
Transmembranei30 – 50HelicalSequence analysisAdd BLAST21
Transmembranei55 – 75HelicalSequence analysisAdd BLAST21
Topological domaini76 – 689CytoplasmicBy similarityAdd BLAST614

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001932141 – 689Bile acyl-CoA synthetaseAdd BLAST689

Proteomic databases

MaxQBiQ4LDG0
PaxDbiQ4LDG0
PeptideAtlasiQ4LDG0
PRIDEiQ4LDG0

PTM databases

iPTMnetiQ4LDG0
PhosphoSitePlusiQ4LDG0
SwissPalmiQ4LDG0

Expressioni

Tissue specificityi

In liver expressed in a periportal distribution.1 Publication

Gene expression databases

BgeeiENSMUSG00000030382
ExpressionAtlasiQ4LDG0 baseline and differential
GenevisibleiQ4LDG0 MM

Interactioni

Protein-protein interaction databases

IntActiQ4LDG0, 2 interactors
MINTiQ4LDG0
STRINGi10090.ENSMUSP00000032539

Structurei

3D structure databases

ProteinModelPortaliQ4LDG0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1179 Eukaryota
ENOG410XQ8T LUCA
GeneTreeiENSGT00550000074420
HOGENOMiHOG000044189
HOVERGENiHBG005642
InParanoidiQ4LDG0
KOiK08748
OMAiDCRQHGV
OrthoDBiEOG091G0B76
PhylomeDBiQ4LDG0
TreeFamiTF313430

Family and domain databases

InterProiView protein in InterPro
IPR025110 AMP-bd_C
IPR020845 AMP-binding_CS
IPR000873 AMP-dep_Synth/Lig
IPR030302 FATP5
PANTHERiPTHR43107:SF4 PTHR43107:SF4, 1 hit
PfamiView protein in Pfam
PF00501 AMP-binding, 1 hit
PF13193 AMP-binding_C, 1 hit
PROSITEiView protein in PROSITE
PS00455 AMP_BINDING, 1 hit

Sequencei

Sequence statusi: Complete.

Q4LDG0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGIWKKLTLL LLLLLLVGLG QPPWPAAMAL ALRWFLGDPT CLVLLGLALL
60 70 80 90 100
GRPWISSWMP HWLSLVGAAL TLFLLPLQPP PGLRWLHKDV AFTFKMLFYG
110 120 130 140 150
LKFRRRLNKH PPETFVDALE RQALAWPDRV ALVCTGSEGS SITNSQLDAR
160 170 180 190 200
SCQAAWVLKA KLKDAVIQNT RDAAAILVLP SKTISALSVF LGLAKLGCPV
210 220 230 240 250
AWINPHSRGM PLLHSVRSSG ASVLIVDPDL QENLEEVLPK LLAENIHCFY
260 270 280 290 300
LGHSSPTPGV EALGASLDAA PSDPVPASLR ATIKWKSPAI FIFTSGTTGL
310 320 330 340 350
PKPAILSHER VIQVSNVLSF CGCRADDVVY DVLPLYHTIG LVLGFLGCLQ
360 370 380 390 400
VGATCVLAPK FSASRFWAEC RQHGVTVILY VGEILRYLCN VPEQPEDKIH
410 420 430 440 450
TVRLAMGNGL RANVWKNFQQ RFGPIRIWEF YGSTEGNVGL MNYVGHCGAV
460 470 480 490 500
GRTSCILRML TPFELVQFDI ETAEPLRDKQ GFCIPVEPGK PGLLLTKVRK
510 520 530 540 550
NQPFLGYRGS QAESNRKLVA NVRRVGDLYF NTGDVLTLDQ EGFFYFQDRL
560 570 580 590 600
GDTFRWKGEN VSTGEVECVL SSLDFLEEVN VYGVPVPGCE GKVGMAAVKL
610 620 630 640 650
APGKTFDGQK LYQHVRSWLP AYATPHFIRI QDSLEITNTY KLVKSRLVRE
660 670 680
GFDVGIIADP LYILDNKAQT FRSLMPDVYQ AVCEGTWNL
Length:689
Mass (Da):76,203
Last modified:November 22, 2005 - v2
Checksum:i1642BBC2CF04FAA3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti88K → I in CAA11688 (PubMed:9642112).Curated1
Sequence conflicti408N → T in AAC40189 (PubMed:9671728).Curated1
Sequence conflicti568C → S in CAA11688 (PubMed:9642112).Curated1
Sequence conflicti688N → K in CAA11688 (PubMed:9642112).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ223959 mRNA Translation: CAA11688.1
BC013335 mRNA Translation: AAH13335.1
BC013272 mRNA Translation: AAH13272.1
BC145823 mRNA Translation: AAI45824.1
BC145825 mRNA Translation: AAI45826.1
AF072760 mRNA Translation: AAC40189.1
CCDSiCCDS20821.1
PIRiJW0107
RefSeqiNP_033538.2, NM_009512.2
UniGeneiMm.10984

Genome annotation databases

EnsembliENSMUST00000032539; ENSMUSP00000032539; ENSMUSG00000030382
GeneIDi26459
KEGGimmu:26459
UCSCiuc009fey.3 mouse

Similar proteinsi

Entry informationi

Entry nameiS27A5_MOUSE
AccessioniPrimary (citable) accession number: Q4LDG0
Secondary accession number(s): A6H6C1, O88694, Q91VD5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: November 22, 2005
Last modified: May 23, 2018
This is version 111 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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