Reviewed, UniProtKB/Swiss-Prot Q4LB23 (METK2_HORVU)

Names and origin

Protein names

Recommended name:
    S-adenosylmethionine synthetase 2
      Short name=AdoMet synthetase 2
    EC=2.5.1.6
Alternative name(s):
    Methionine adenosyltransferase 2
      Short name=MAT 2

Gene names

Name:

SAM2

Organism

Hordeum vulgare (Barley)

Taxonomic identifier

4513 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Hordeum

Protein attributes

Sequence length	394 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme By similarity.
Catalytic activity	ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine.
Cofactor	Binds 2 divalent ions per subunit. Magnesium or cobalt By similarity. Binds 1 potassium ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the AdoMet synthetase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	ATP-binding Cobalt Magnesium Metal-binding Nucleotide-binding Potassium
Molecular function	Transferase
Gene Ontology (GO)
Biological process	one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cobalt ion binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW methionine adenosyltransferase activity Inferred from electronic annotation. Source: EC potassium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 394	394	S-adenosylmethionine synthetase 2		PRO_0000363025
Regions
	Nucleotide binding	121 – 126	6	ATP Potential
	Nucleotide binding	269 – 276	8	ATP Potential
Sites
	Metal binding	19	1	Magnesium By similarity
	Metal binding	45	1	Potassium By similarity
	Metal binding	273	1	Potassium By similarity
	Metal binding	281	1	Magnesium By similarity
	Binding site	149	1	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q4LB23-1 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: E5F081812EAB2E34	FASTA	394	42,828
	10 20 30 40 50 60 MAAETFLFTS ESVNEGHPDK LCDQVSDAVL DACLAQDPDS KVACETCTKT NMVMVFGEIT 70 80 90 100 110 120 TKATVDYEKI VRDTCRDIGF ISDDVGLDAD HCKVLVNIEQ QSPDIAQGVH GHFTKRPEEV 130 140 150 160 170 180 GAGDQGIMFG YATDETPELM PLTHMLATKL GARLTEVRKN GTCAWLRPDG KTQVTIEYLN 190 200 210 220 230 240 EGGAMVPVRV HTVLISTQHD ETVTNDEIAA DLKEHVIKPV IPGKYLDENT IFHLNPSGRF 250 260 270 280 290 300 VIGGPHGDAG LTGRKIIIDT YGGWGAHGGG AFSGKDPTKV DRSGAYIARQ AAKSIIASGL 310 320 330 340 350 360 ARRCIVQISY AIGVPEPLSV FVDSYGTGKI PDREILKLVK ENFDFRPGMI SINLDLKKGG 370 380 390 KRFIKTAAYG HFGRDDADFT WEVVKPLKFD KASA

References

[1]	"The methylation cycle and its possible functions in barley endosperm development." Radchuk V.V., Sreenivasulu N., Radchuk R.I., Wobus U., Weschke W. Plant Mol. Biol. 59:289-307(2005) [PubMed: 16247558] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Barke. Tissue: Seed.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM039894 mRNA. Translation: CAJ01703.1.
3D structure databases
ModBase	Search...
Organism-specific databases
Gramene	Q4LB23.
Gene expression databases
Genevestigator	Q4LB23.
Family and domain databases
InterPro	IPR002133. S-AdoMet_synthetase. [Graphical view]
PANTHER	PTHR11964. S-AdoMet_synt. 1 hit.
Pfam	PF02773. S-AdoMet_synt_C. 1 hit. PF02772. S-AdoMet_synt_M. 1 hit. PF00438. S-AdoMet_synt_N. 1 hit. [Graphical view]
PIRSF	PIRSF000497. MAT. 1 hit.
TIGRFAMs	TIGR01034. metK. 1 hit.
PROSITE	PS00376. ADOMET_SYNTHETASE_1. 1 hit. PS00377. ADOMET_SYNTHETASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

METK2_HORVU

Accession

Primary (citable) accession number: Q4LB23

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	August 2, 2005
Last modified:	October 13, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)