Q4LB21 (METK4_HORVU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
S-adenosylmethionine synthase 4
Short name=AdoMet synthase 4
EC=2.5.1.6

Alternative name(s):
Methionine adenosyltransferase 4
Short name=MAT 4

Gene names

Name:

SAM4

Organism

Hordeum vulgare (Barley)

Taxonomic identifier

4513 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Pooideae › Triticeae › Hordeum

Protein attributes

Sequence length	396 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the enzyme By similarity.
Catalytic activity	ATP + L-methionine + H2O = phosphate + diphosphate + S-adenosyl-L-methionine.
Cofactor	Binds 2 divalent ions per subunit. Magnesium or cobalt By similarity. Binds 1 potassium ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the AdoMet synthase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	ATP-binding Cobalt Magnesium Metal-binding Nucleotide-binding Potassium
Molecular function	Transferase
Gene Ontology (GO)
Biological process	S-adenosylmethionine biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW methionine adenosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 396	396	S-adenosylmethionine synthase 4		PRO_0000363027
Regions
	Nucleotide binding	122 – 127	6	ATP Potential
	Nucleotide binding	270 – 277	8	ATP Potential
Sites
	Metal binding	20	1	Magnesium By similarity
	Metal binding	46	1	Potassium By similarity
	Metal binding	274	1	Potassium By similarity
	Metal binding	282	1	Magnesium By similarity
	Binding site	150	1	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q4LB21 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: 2FE018D9D57ABB58	FASTA	396	43,211
	10 20 30 40 50 60 MAEVETFLFT SESVNEGHPD KLCDQISDAV LDACLAEDPD SKVACETCTK TNMVMVFGEI 70 80 90 100 110 120 TTKANVDYEK IVRDTCRGIG FVSNDVGLDA DHCKVLVNIE QQSPDIAQGV HGHFTKRPEE 130 140 150 160 170 180 IGAGDQGHMF GYATDETPEF MPLSHVLATK LGARLTEVRK NATCPWLRPD GKTQVTVEYH 190 200 210 220 230 240 NDNGAMVPIR VHTVLISTQH DETVTNDEIA ADLKEHVIKP VIPEQYLDEN TIFHLNPSGR 250 260 270 280 290 300 FVIGGPHGDA GLTGRKIIID TYGGWGAHGG GAFSGKDPTK VDRSGAYIAR QAAKSIVASG 310 320 330 340 350 360 IARRCIVQVS YAIGVPEPLS VFVDTYGTGK IPDKEILEIV KENFDFRPGM IIINLDLKRG 370 380 390 GSGRYLKTAA YGHFGRDGAD FTWEVVKPLK WEKPSA

References

[1]	"The methylation cycle and its possible functions in barley endosperm development." Radchuk V.V., Sreenivasulu N., Radchuk R.I., Wobus U., Weschke W. Plant Mol. Biol. 59:289-307(2005) [PubMed: 16247558] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Barke. Tissue: Seed.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM039896 mRNA. Translation: CAJ01705.1.
3D structure databases
HSSP	HSSP built from PDB template 1QM4 based on UniProtKB P13444.
ProteinModelPortal	Q4LB21.
SMR	Q4LB21. Positions 6-391.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
Gramene	Q4LB21.
Gene expression databases
Genevestigator	Q4LB21.
Family and domain databases
InterPro	IPR022631. ADOMET_SYNTHASE_CS. IPR022630. S-AdoMet_synt_C. IPR022629. S-AdoMet_synt_central. IPR022628. S-AdoMet_synt_N. IPR002133. S-AdoMet_synthetase. IPR022636. S-AdoMet_synthetase_sfam. [Graphical view]
PANTHER	PTHR11964. S-AdoMet_synt. 1 hit.
Pfam	PF02773. S-AdoMet_synt_C. 1 hit. PF02772. S-AdoMet_synt_M. 1 hit. PF00438. S-AdoMet_synt_N. 1 hit. [Graphical view]
PIRSF	PIRSF000497. MAT. 1 hit.
SUPFAM	SSF55973. S-AdoMet_synt. 3 hits.
TIGRFAMs	TIGR01034. MetK. 1 hit.
PROSITE	PS00376. ADOMET_SYNTHASE_1. 1 hit. PS00377. ADOMET_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

METK4_HORVU

Accession

Primary (citable) accession number: Q4LB21

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	August 2, 2005
Last modified:	June 28, 2011
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program