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Protein

3-methyl-2-oxobutanoate hydroxymethyltransferase

Gene

panB

Organism
Staphylococcus haemolyticus (strain JCSC1435)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is transferred onto alpha-ketoisovalerate to form ketopantoate.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = tetrahydrofolate + 2-dehydropantoate.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: (R)-pantothenate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes (R)-pantoate from 3-methyl-2-oxobutanoate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB)
  2. 2-dehydropantoate 2-reductase (SH0615), 2-dehydropantoate 2-reductase (SH0180)
This subpathway is part of the pathway (R)-pantothenate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-pantoate from 3-methyl-2-oxobutanoate, the pathway (R)-pantothenate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi43MagnesiumUniRule annotation1
Metal bindingi82MagnesiumUniRule annotation1
Binding sitei82Alpha-ketoisovalerateUniRule annotation1
Binding sitei112Alpha-ketoisovalerateUniRule annotation1
Metal bindingi114MagnesiumUniRule annotation1
Active sitei179Proton acceptorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processPantothenate biosynthesis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciSHAE279808:G1G27-168-MONOMER
UniPathwayiUPA00028; UER00003

Names & Taxonomyi

Protein namesi
Recommended name:
3-methyl-2-oxobutanoate hydroxymethyltransferaseUniRule annotation (EC:2.1.2.11UniRule annotation)
Alternative name(s):
Ketopantoate hydroxymethyltransferaseUniRule annotation
Short name:
KPHMTUniRule annotation
Gene namesi
Name:panBUniRule annotation
Ordered Locus Names:SH0181
OrganismiStaphylococcus haemolyticus (strain JCSC1435)
Taxonomic identifieri279808 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000000543 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002973861 – 2723-methyl-2-oxobutanoate hydroxymethyltransferaseAdd BLAST272

Interactioni

Subunit structurei

Homodecamer; pentamer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi279808.SH0181

Structurei

3D structure databases

ProteinModelPortaliQ4LA35
SMRiQ4LA35
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni43 – 44Alpha-ketoisovalerate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the PanB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CCG Bacteria
COG0413 LUCA
HOGENOMiHOG000078427
KOiK00606
OMAiGHIGLMP
OrthoDBiPOG091H02K8

Family and domain databases

CDDicd06557 KPHMT-like, 1 hit
HAMAPiMF_00156 PanB, 1 hit
InterProiView protein in InterPro
IPR003700 Pantoate_hydroxy_MeTrfase
IPR015813 Pyrv/PenolPyrv_Kinase-like_dom
PANTHERiPTHR20881 PTHR20881, 1 hit
PfamiView protein in Pfam
PF02548 Pantoate_transf, 1 hit
PIRSFiPIRSF000388 Pantoate_hydroxy_MeTrfase, 1 hit
SUPFAMiSSF51621 SSF51621, 1 hit
TIGRFAMsiTIGR00222 panB, 1 hit

Sequencei

Sequence statusi: Complete.

Q4LA35-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNLAKLVEM KQDKTKISMI TAYDFPSAKQ AQDANIDMIL VGDSLGMTVL
60 70 80 90 100
GYETTTQVTL EDMIHHGKAV RRGADDTFVV VDLPIGTVGV SDEHDLKNAI
110 120 130 140 150
TLYQETNANA LKAEGAHLVN FIKKSTQIGI PIVAHLGLMP QSVGVMGYRL
160 170 180 190 200
QGSTKDAAEQ LIQEAHAVQE AGAVALVLEA IPSDLAGLIS EQLDIPVIGI
210 220 230 240 250
GAGKDTDGQV LVYHDMLNYG VDRYAKFVKQ FGDFSVGIDA IKHYDEEVKA
260 270
GTFPAEAHTY KKKILNEVNS ND
Length:272
Mass (Da):29,426
Last modified:August 2, 2005 - v1
Checksum:i1A5E3F62B89FCF37
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006716 Genomic DNA Translation: BAE03490.1
RefSeqiWP_011274510.1, NC_007168.1

Genome annotation databases

EnsemblBacteriaiBAE03490; BAE03490; SH0181
GeneIDi24247991
KEGGisha:SH0181

Similar proteinsi

Entry informationi

Entry nameiPANB_STAHJ
AccessioniPrimary (citable) accession number: Q4LA35
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: August 2, 2005
Last modified: April 25, 2018
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health