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Q4L9U7

- BIOB_STAHJ

UniProt

Q4L9U7 - BIOB_STAHJ

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Protein

Biotin synthase

Gene
bioB, SH0269
Organism
Staphylococcus haemolyticus (strain JCSC1435)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi63 – 631Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi67 – 671Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi70 – 701Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi106 – 1061Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi139 – 1391Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi199 – 1991Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi269 – 2691Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciSHAE279808:GJX7-271-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:SH0269
OrganismiStaphylococcus haemolyticus (strain JCSC1435)
Taxonomic identifieri279808 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
ProteomesiUP000000543: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 321321Biotin synthaseUniRule annotationPRO_0000381659Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi279808.SH0269.

Structurei

3D structure databases

ProteinModelPortaliQ4L9U7.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239958.
KOiK01012.
OMAiDETQALC.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q4L9U7-1 [UniParc]FASTAAdd to Basket

« Hide

MRFNLAERIL NQDVLSKDEA LALFEDETID TFELLNEAYI IRKHFFGKKV    50
KLNMILNAKS GICSEDCGYC GQSVKMKEKQ RYALVEPDKI KAGAQVATEN 100
HIGTYCIVMS GRGPTNREVD HICETVHDIK VLHPQLKICA CLGLTNEEQA 150
EKLKEAGVDR YNHNLNTSER YHNEVVTTHT YEDRVRTVEI MKANHISPCS 200
GVICGMGETN EDIIDMAFAL REIDADSIPI NFLHPIKGTK FGGLDLLSPM 250
KCLRIIAMFR LINPSKEIRI AGGREVNLRS LQAIALKAAN SIFVGDYLIT 300
GGQPNELDYQ MIEDLGFEID G 321
Length:321
Mass (Da):36,007
Last modified:August 2, 2005 - v1
Checksum:iDAF3F0A2BEF0DFF5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP006716 Genomic DNA. Translation: BAE03578.1.
RefSeqiYP_252184.1. NC_007168.1.

Genome annotation databases

EnsemblBacteriaiBAE03578; BAE03578; SH0269.
GeneIDi3482382.
KEGGisha:SH0269.
PATRICi19616567. VBIStaHae67511_0260.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AP006716 Genomic DNA. Translation: BAE03578.1 .
RefSeqi YP_252184.1. NC_007168.1.

3D structure databases

ProteinModelPortali Q4L9U7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 279808.SH0269.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAE03578 ; BAE03578 ; SH0269 .
GeneIDi 3482382.
KEGGi sha:SH0269.
PATRICi 19616567. VBIStaHae67511_0260.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239958.
KOi K01012.
OMAi DETQALC.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci SHAE279808:GJX7-271-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Whole-genome sequencing of Staphylococcus haemolyticus uncovers the extreme plasticity of its genome and the evolution of human-colonizing staphylococcal species."
    Takeuchi F., Watanabe S., Baba T., Yuzawa H., Ito T., Morimoto Y., Kuroda M., Cui L., Takahashi M., Ankai A., Baba S., Fukui S., Lee J.C., Hiramatsu K.
    J. Bacteriol. 187:7292-7308(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JCSC1435.

Entry informationi

Entry nameiBIOB_STAHJ
AccessioniPrimary (citable) accession number: Q4L9U7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: August 2, 2005
Last modified: May 14, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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