Reviewed, UniProtKB/Swiss-Prot Q4L9F3 (CYSH_STAHJ)

Names and origin

Protein names

Recommended name:
    Phosphoadenosine phosphosulfate reductase
    EC=1.8.4.8
Alternative name(s):
    PAPS reductase, thioredoxin dependent
    PAdoPS reductase
    3'-phosphoadenylylsulfate reductase
    PAPS sulfotransferase

Gene names

Name:	cysH
Ordered Locus Names:	SH0413

Organism

Staphylococcus haemolyticus (strain JCSC1435) [Complete proteome] [HAMAP]

Taxonomic identifier

279808 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	243 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Reduction of activated sulfate into sulfite. HAMAP MF_00063
Catalytic activity	Adenosine 3',5'-bisphosphate + sulfite + thioredoxin disulfide = 3'-phosphoadenylyl sulfate + thioredoxin. HAMAP MF_00063
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; sulfite from sulfate: step 3/3. HAMAP MF_00063
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the PAPS reductase family. CysH subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteine biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW sulfate assimilation, phosphoadenylyl sulfate reduction by phosphoadenylyl-sulfate reductase (thioredoxin) Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	phosphoadenylyl-sulfate reductase (thioredoxin) activity Inferred from electronic annotation. Source: HAMAP transferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 243	243	Phosphoadenosine phosphosulfate reductase HAMAP MF_00063		PRO_1000008941

Sequences

	Sequence		Length	Mass (Da)
	Q4L9F3-1 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: 27E3DAA32EE97BC4	FASTA	243	28,093
	10 20 30 40 50 60 MSVPTITYEN FEGDPFIDSI STDDATKGAY EILEWAYRTY GDSIVYSCSF GAESMVLIDL 70 80 90 100 110 120 IYQIKPDAQI VFLDTDLHFQ ETYDLIDRVK EHFPKLRIEM KKPDLTLEEQ ADKYNPALWK 130 140 150 160 170 180 NNPNQCCYIR KIKPLEEVLG GAVAWVSGLR RDQSPTRANT NFINKDERFK SVKVCPLIYW 190 200 210 220 230 240 TEDEVWDYIK KHDLPYNALH DQHYPSIGCI PCTAPVFDSE DSRAGRWSNF DKTECGLHVA DKP

References

[1]

"Whole-genome sequencing of Staphylococcus haemolyticus uncovers the extreme plasticity of its genome and the evolution of human-colonizing staphylococcal species." Takeuchi F., Watanabe S., Baba T., Yuzawa H., Ito T., Morimoto Y., Kuroda M., Cui L., Takahashi M., Ankai A., Baba S., Fukui S., Lee J.C., Hiramatsu K. J. Bacteriol. 187:7292-7308(2005) [PubMed: 16237012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP006716 Genomic DNA. Translation: BAE03722.1.
RefSeq	YP_252328.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3484069.
GenomeReviews	Gene locus SH0413 in contig AP006716_GR.
KEGG	sha:SH0413.
NMPDR	fig|279808.3.peg.482.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q4L9F3.
OMA	Q4L9F3. TRFNGLK.
Enzyme and pathway databases
BioCyc	SHAE279808:SH0413-MON.
Family and domain databases
HAMAP	MF_00063. [Tree]
InterPro	IPR011798. APS_reductase. IPR004511. PAdo_PSO4_Rdtase_CysH. IPR002500. PAPS_reduct. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF01507. PAPS_reduct. 1 hit. [Graphical view]
TIGRFAMs	TIGR02055. APS_reductase. 1 hit. TIGR00434. cysH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CYSH_STAHJ

Accession

Primary (citable) accession number: Q4L9F3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	August 2, 2005
Last modified:	June 16, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)