Reviewed, UniProtKB/Swiss-Prot Q4L9D7 (BETA_STAHJ)

Names and origin

Protein names

Recommended name:
    Choline dehydrogenase
      Short name=CHD
      Short name=CDH
    EC=1.1.99.1

Gene names

Name:	betA
Ordered Locus Names:	SH0429

Organism

Staphylococcus haemolyticus (strain JCSC1435) [Complete proteome] [HAMAP]

Taxonomic identifier

279808 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	568 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Can catalyze the oxidation of choline to betaine aldehyde and betaine aldehyde to glycine betaine By similarity.
Catalytic activity	Choline + acceptor = betaine aldehyde + reduced acceptor. HAMAP MF_00750
Cofactor	FAD By similarity.
Pathway	Amine and polyamine biosynthesis; betaine biosynthesis via choline pathway; betaine aldehyde from choline (cytochrome c reductase route): step 1/1. HAMAP MF_00750
Sequence similarities	Belongs to the GMC oxidoreductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular alcohol metabolic process Inferred from electronic annotation. Source: InterPro glycine betaine biosynthetic process from choline Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro choline dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 568	568	Choline dehydrogenase HAMAP MF_00750		PRO_0000205603
Regions
	Nucleotide binding	8 – 37	30	FAD Probable
Sites
	Active site	473	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4L9D7-1 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: AAE7A8FC49494CA7	FASTA	568	63,553
	10 20 30 40 50 60 MSKKNSYDYV IIGGGSAGSV LGNRLTEDKD KEVLVLEAGR SDYPWDLFIQ MPAALMFPSG 70 80 90 100 110 120 NRFYDWIYQT EEEPHMGRKV DHARGKVLGG SSSINGMIYQ RGNPMDYEGW AEPEGMESWD 130 140 150 160 170 180 FAHCLPYFKR LEKTYGATPF DQFRGHHGPI KLKRGPATNP LFKSFFDAGV EAGYHKTKDV 190 200 210 220 230 240 NGYRQEGFGP FDSQVHNGRR VSASRAYLHP AMKRKNLTVK TRAFVTKIHF DGNKATGVTF 250 260 270 280 290 300 KRNGRYHTVD AGEVILSGGA FNTPQLLQLS GIGDAEFLKS KGIEPRMHLP GVGENFEDHL 310 320 330 340 350 360 EVYIQHECKE PVSLQPSLDV KRMPWIGLQW IFARKGAAAS NHFEGGAFVR SNNQVAYPNL 370 380 390 400 410 420 MFHFLPIAVR YDGQKAPVAH GYQVHVGPMY SNSRGSLKIK SKDPFEKPSI VFNYLSTKED 430 440 450 460 470 480 EQEWVEAIRV ARNILAQKAM DPYNGGEISP GPSVQTDEEI LDWVRRDGET ALHPSCSAKM 490 500 510 520 530 540 GPASDPMSVV DPLTMKVHGM ENLRVVDASA MPRTTNGNIH APVLMLAEKA ADIIRGKKPL 550 560 EPQYVDYYKH GVSDENAGAM EFDPYYQH

References

[1]

"Whole-genome sequencing of Staphylococcus haemolyticus uncovers the extreme plasticity of its genome and the evolution of human-colonizing staphylococcal species." Takeuchi F., Watanabe S., Baba T., Yuzawa H., Ito T., Morimoto Y., Kuroda M., Cui L., Takahashi M., Ankai A., Baba S., Fukui S., Lee J.C., Hiramatsu K. J. Bacteriol. 187:7292-7308(2005) [PubMed: 16237012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP006716 Genomic DNA. Translation: BAE03738.1.
RefSeq	YP_252344.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3482376.
GenomeReviews	Gene locus SH0429 in contig AP006716_GR.
KEGG	sha:SH0429.
NMPDR	fig|279808.3.peg.518.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q4L9D7.
OMA	Q4L9D7. AGRSDYP.
Enzyme and pathway databases
BioCyc	SHAE279808:SH0429-MON.
Family and domain databases
HAMAP	MF_00750. [Tree]
InterPro	IPR011533. Choline_dehydrogenase. IPR012132. GMC_OxRdtase. IPR000172. GMC_OxRdtase_N. IPR007867. GMC_OxRtase_C. [Graphical view]
Pfam	PF05199. GMC_oxred_C. 1 hit. PF00732. GMC_oxred_N. 1 hit. [Graphical view]
PIRSF	PIRSF000137. Alcohol_oxidase. 1 hit.
TIGRFAMs	TIGR01810. betA. 1 hit.
PROSITE	PS00623. GMC_OXRED_1. 1 hit. PS00624. GMC_OXRED_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BETA_STAHJ

Accession

Primary (citable) accession number: Q4L9D7

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	August 2, 2005
Last modified:	June 16, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)