Reviewed, UniProtKB/Swiss-Prot Q4L966 (ROCA_STAHJ)

Names and origin

Protein names

Recommended name:
    1-pyrroline-5-carboxylate dehydrogenase
      Short name=P5C dehydrogenase
    EC=1.5.1.12

Gene names

Name:	rocA
Ordered Locus Names:	SH0500

Organism

Staphylococcus haemolyticus (strain JCSC1435) [Complete proteome] [HAMAP]

Taxonomic identifier

279808 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	514 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-1-pyrroline-5-carboxylate + NAD(P)+ + 2 H2O = L-glutamate + NAD(P)H. HAMAP MF_00733
Pathway	Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 2/2. HAMAP MF_00733
Sequence similarities	Belongs to the aldehyde dehydrogenase family. RocA subfamily.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamate biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	1-pyrroline-5-carboxylate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 514	514	1-pyrroline-5-carboxylate dehydrogenase HAMAP MF_00733		PRO_0000056522
Sites
	Active site	286	1	By similarity
	Active site	320	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4L966-1 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: 41AAD6873F1C7C86	FASTA	514	56,597
	10 20 30 40 50 60 MVVPFKNEPG IDFSVQENVE RFQKTLEQVK NELGQTLPIV IDGEHITKDD TFDSINPANT 70 80 90 100 110 120 SELIAKVSKA TKEDVDKAFE SSNKAYKAWR QWSHKDRAEL LLRVAAIIRR RKEEISAVMV 130 140 150 160 170 180 YEAGKPWDEA VGDAAEGIDF IEYYARSMME LADGKPVLDR EGEHNKYFYK SIGTGVTIPP 190 200 210 220 230 240 WNFPFAIMAG TTLAPVVAGN TVLLKPAEDT VLTAYKLIEI LEEAGLPKGV VNFVPGDPKE 250 260 270 280 290 300 IGDYLVDSVH THFVTFTGSR ATGTRIYERS AVVQEGQTFL KRVIAEMGGK DAIVVDENID 310 320 330 340 350 360 TDLAAESIIT SAFGFSGQKC SACSRAIVHS SVYDEVLEKA VALTKELTVG NTVDNTFMGP 370 380 390 400 410 420 VINKKQFDKI KKYIEIGGKE GKIEIGGEAD DSTGYFIKPT IISGLKSSDQ VMQEEIFGPV 430 440 450 460 470 480 VGFTKFDNFE EAIEIANDTD YGLTGAVITN NRENWIKAVN EFDVGNLYLN RGCTAAVVGY 490 500 510 HPFGGFKMSG TDAKTGSPDY LLNFLEQKVV SEMF

References

[1]

"Whole-genome sequencing of Staphylococcus haemolyticus uncovers the extreme plasticity of its genome and the evolution of human-colonizing staphylococcal species." Takeuchi F., Watanabe S., Baba T., Yuzawa H., Ito T., Morimoto Y., Kuroda M., Cui L., Takahashi M., Ankai A., Baba S., Fukui S., Lee J.C., Hiramatsu K. J. Bacteriol. 187:7292-7308(2005) [PubMed: 16237012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP006716 Genomic DNA. Translation: BAE03809.1.
RefSeq	YP_252415.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q4L966.
Genome annotation databases
GeneID	3481891.
GenomeReviews	Gene locus SH0500 in contig AP006716_GR.
KEGG	sha:SH0500.
NMPDR	fig|279808.3.peg.589.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q4L966.
OMA	SINPANT.
Enzyme and pathway databases
BioCyc	SHAE279808:SH0500-MON.
Family and domain databases
HAMAP	MF_00733. [Tree]
InterPro	IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR005932. d-1-pyrroline-5-COlate_DH-2. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
TIGRFAMs	TIGR01237. D1pyr5carbox2. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ROCA_STAHJ

Accession

Primary (citable) accession number: Q4L966

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 22, 2005
Last sequence update:	August 2, 2005
Last modified:	November 3, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)