Reviewed, UniProtKB/Swiss-Prot Q4L945 (PTG3C_STAHJ)

Names and origin

Protein names

Recommended name:
    PTS system glucose-specific EIICBA component
Alternative name(s):
    EIICBA-Glc
      Short name=EII-Glc
Including the following 3 domains:
    1- Recommended name:
            Glucose permease IIC component
        Alternative name(s):
            PTS system glucose-specific EIIC component
    2- Recommended name:
            Glucose-specific phosphotransferase enzyme IIB component
              EC=2.7.1.69
        Alternative name(s):
            PTS system glucose-specific EIIB component
    3- Recommended name:
            Glucose-specific phosphotransferase enzyme IIA component
              EC=2.7.1.-
        Alternative name(s):
            PTS system glucose-specific EIIA component

Gene names

Name:	ptsG
Ordered Locus Names:	SH0521

Organism

Staphylococcus haemolyticus (strain JCSC1435) [Complete proteome] [HAMAP]

Taxonomic identifier

279808 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	675 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in glucose transport By similarity.
Catalytic activity	Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate. Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine.
Subcellular location	Cell membrane; Multi-pass membrane protein By similarity.
Domain	The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site. The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain. The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.
Sequence similarities	Contains 1 PTS EIIA type-1 domain. Contains 1 PTS EIIB type-1 domain. Contains 1 PTS EIIC type-1 domain.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose transport Inferred from electronic annotation. Source: InterPro phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	glucose transmembrane transporter activity Inferred from electronic annotation. Source: InterPro kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred from electronic annotation. Source: EC sugar:hydrogen symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 675	675	PTS system glucose-specific EIICBA component		PRO_0000351405
Regions
	Transmembrane	16 – 36	21	Potential
	Transmembrane	59 – 79	21	Potential
	Transmembrane	81 – 101	21	Potential
	Transmembrane	126 – 146	21	Potential
	Transmembrane	173 – 193	21	Potential
	Transmembrane	199 – 219	21	Potential
	Transmembrane	273 – 293	21	Potential
	Transmembrane	303 – 323	21	Potential
	Transmembrane	328 – 348	21	Potential
	Transmembrane	355 – 375	21	Potential
	Transmembrane	378 – 398	21	Potential
	Domain	3 – 414	412	PTS EIIC type-1
	Domain	425 – 506	82	PTS EIIB type-1
	Domain	547 – 651	105	PTS EIIA type-1
Sites
	Active site	447	1	Phosphocysteine intermediate; for EIIB activity By similarity
	Active site	599	1	Tele-phosphohistidine intermediate; for EIIA activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4L945-1 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: 6119AECA0C7FFB6D	FASTA	675	73,214
	10 20 30 40 50 60 MFKKLFGQLQ RIGKALMLPV AILPAAGLLL AIGTAFQGEA LQQYLPFIKN GVIQNIANMM 70 80 90 100 110 120 TGAGGIIFDN LPIIFALGVA IGLAGGDGVA AIAAFVGFII MNKTMGAFLN VTPAQLEDPS 130 140 150 160 170 180 KGFANVLGIP TLQTGVFGGI IIGALAAWCY NKFYNISLPS YLGFFAGKRF VPIMMATTSF 190 200 210 220 230 240 ILAFPMAWIW PFIQNGLNAF STGLLDSNTG LAVFLFGFIK RLLIPFGLHH IFHAPFWFEF 250 260 270 280 290 300 GSWKNAAGEI IRGDQRIFIE QIREGVHLTS GKFMQGEFPV MMFGLPAAAL AIYQTAKPEN 310 320 330 340 350 360 KKVVGGLMLS AALTSFLTGI TEPLEFSFLF VAPLLFFIHA VLDGLSFLTL YLLNLHLGYT 370 380 390 400 410 420 FSGGFIDFVL LGILPNKTPW WLVIPVGLVY AVIYYVVFRF LIVKFNFKTP GREDKQASVA 430 440 450 460 470 480 NTSASKLPFD VLDAMGGKEN IKHLDACITR LRVEVNDKSK VDVEGLKALG ASGVLEVGNN 490 500 510 520 530 540 MQAIFGPKSD QIKHDMARIM NGDITKPSET TVTEDTSDEP VQLEEVKETD IYAPGTGHII 550 560 570 580 590 600 PLSEVPDKVF SEKMMGDGIG FVPEKGGIVA PFDGTVKTIF PTKHAIGLES DTGIEVLIHI 610 620 630 640 650 660 GIDTVKLNGE GFESLVDVNE PVTQGQPLMK INLAYLKEHA PSVVTPVIIT NQGDKTLTFD 670 DVDSVDPGKR IMTIK

References

[1]

"Whole-genome sequencing of Staphylococcus haemolyticus uncovers the extreme plasticity of its genome and the evolution of human-colonizing staphylococcal species." Takeuchi F., Watanabe S., Baba T., Yuzawa H., Ito T., Morimoto Y., Kuroda M., Cui L., Takahashi M., Ankai A., Baba S., Fukui S., Lee J.C., Hiramatsu K. J. Bacteriol. 187:7292-7308(2005) [PubMed: 16237012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006716 Genomic DNA. Translation: BAE03830.1.
RefSeq	YP_252436.1.
3D structure databases
HSSP	HSSP built from PDB template 1IBA based on UniProtKB P69786.
SMR	Q4L945. Positions 434-496, 524-675.
ModBase	Search...
Protein-protein interaction databases
STRING	Q4L945.
Genome annotation databases
GeneID	3481950.
GenomeReviews	Gene locus SH0521 in contig AP006716_GR.
KEGG	sha:SH0521.
NMPDR	fig|279808.3.peg.610.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1263.
HOGENOM	HBG571563.
OMA	CIPAIAL.
PhylomeDB	Q4L945.
Enzyme and pathway databases
BioCyc	SHAE279808:SH0521-MONOMER.
Family and domain databases
InterPro	IPR011055. Dup_hybrid_motif. IPR018113. PTrfase_EIIB/Cys_phosph_CS. IPR001127. PTS_EIIA_1_perm. IPR001996. PTS_EIIB_1. IPR003352. PTS_EIIC. IPR013013. PTS_EIIC_1. IPR011535. PTS_Glc-like_IIB_component. IPR011299. PTS_IIBC_glc. [Graphical view]
Gene3D	G3DSA:3.30.1360.60. PTS_EIIB. 1 hit.
Pfam	PF00358. PTS_EIIA_1. 1 hit. PF00367. PTS_EIIB. 1 hit. PF02378. PTS_EIIC. 1 hit. [Graphical view]
TIGRFAMs	TIGR00826. EIIB_glc. 1 hit. TIGR00830. PTBA. 1 hit. TIGR02002. PTS-II-BC-glcB. 1 hit.
PROSITE	PS51093. PTS_EIIA_TYPE_1. 1 hit. PS00371. PTS_EIIA_TYPE_1_HIS. 1 hit. PS51098. PTS_EIIB_TYPE_1. 1 hit. PS01035. PTS_EIIB_TYPE_1_CYS. 1 hit. PS51103. PTS_EIIC_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTG3C_STAHJ

Accession

Primary (citable) accession number: Q4L945

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 14, 2008
Last sequence update:	August 2, 2005
Last modified:	February 9, 2010
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)