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Protein

Oxygen sensor histidine kinase NreB

Gene

nreB

Organism
Staphylococcus haemolyticus (strain JCSC1435)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Member of the two-component regulatory system NreB/NreC involved in the control of dissimilatory nitrate/nitrite reduction in response to oxygen. NreB functions as a direct oxygen sensor histidine kinase which is autophosphorylated, in the absence of oxygen, probably at the conserved histidine residue, and transfers its phosphate group probably to a conserved aspartate residue of NreC. NreB/NreC activates the expression of the nitrate (narGHJI) and nitrite (nir) reductase operons, as well as the putative nitrate transporter gene narT (By similarity).By similarity

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

Cofactori

[4Fe-4S] clusterCuratedNote: Binds 1 [4Fe-4S] cluster.Curated

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi58 – 581Iron-sulfur (4Fe-4S)Sequence analysis
Metal bindingi61 – 611Iron-sulfur (4Fe-4S)Sequence analysis
Metal bindingi73 – 731Iron-sulfur (4Fe-4S)Sequence analysis
Metal bindingi76 – 761Iron-sulfur (4Fe-4S)Sequence analysis

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Two-component regulatory system

Keywords - Ligandi

4Fe-4S, ATP-binding, Iron, Iron-sulfur, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSHAE279808:GJX7-661-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Oxygen sensor histidine kinase NreB (EC:2.7.13.3)
Alternative name(s):
Nitrogen regulation protein B
Gene namesi
Name:nreB
Ordered Locus Names:SH0659
OrganismiStaphylococcus haemolyticus (strain JCSC1435)
Taxonomic identifieri279808 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000000543 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 344344Oxygen sensor histidine kinase NreBPRO_0000349341Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei158 – 1581Phosphohistidine; by autocatalysisPROSITE-ProRule annotation

Post-translational modificationi

Autophosphorylated.By similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Protein-protein interaction databases

STRINGi279808.SH0659.

Structurei

3D structure databases

ProteinModelPortaliQ4L8Q7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini147 – 344198Histidine kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 histidine kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
ENOG410XNMH. LUCA.
HOGENOMiHOG000008986.
KOiK07683.
OMAiRCEGYSN.
OrthoDBiEOG69GZGV.

Family and domain databases

Gene3Di3.30.565.10. 1 hit.
InterProiIPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR004358. Sig_transdc_His_kin-like_C.
IPR011712. Sig_transdc_His_kin_sub3_dim/P.
IPR017203. Sig_transdc_His_kinase_NreB.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
PF07730. HisKA_3. 1 hit.
[Graphical view]
PIRSFiPIRSF037432. STHK_NreB. 1 hit.
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
[Graphical view]
SUPFAMiSSF55874. SSF55874. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4L8Q7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLDVDNLDLE NLLKKYYEKT NEKIVFVNKD GKVIAMNDAA EEIISKDNNY
60 70 80 90 100
SAMTNAICNR CEGYSNEFAL QSCINCYLDT TKPNDMNFQV FMKTVDNKIQ
110 120 130 140 150
PFTASYQCID DEAQIYAFTL QDISPQIERQ EKMYQRQMLR KTIAAQENER
160 170 180 190 200
KRISRELHDS VVQEMLNVDV ELRLLKYQQD MAQLIEKSEH IELLMSNLIN
210 220 230 240 250
DIRDLSVELR PSSLDDLGLE AAFKSYFKQL EYNYGLNVVY QSNIQTIRFD
260 270 280 290 300
SEIETVVYRV VQEAIFNALK YAGVYEVEVT IQQTEEGLIA EIIDRGKGFD
310 320 330 340
PNLKPQGTGL GLYGMNERAE LVKGTVNIET HIGKGTIITL EVPV
Length:344
Mass (Da):39,532
Last modified:August 2, 2005 - v1
Checksum:i9D237D09C3A09476
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006716 Genomic DNA. Translation: BAE03968.1.
RefSeqiWP_011274984.1. NC_007168.1.

Genome annotation databases

EnsemblBacteriaiBAE03968; BAE03968; SH0659.
GeneIDi24246721.
KEGGisha:SH0659.
PATRICi19617341. VBIStaHae67511_0647.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006716 Genomic DNA. Translation: BAE03968.1.
RefSeqiWP_011274984.1. NC_007168.1.

3D structure databases

ProteinModelPortaliQ4L8Q7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi279808.SH0659.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAE03968; BAE03968; SH0659.
GeneIDi24246721.
KEGGisha:SH0659.
PATRICi19617341. VBIStaHae67511_0647.

Phylogenomic databases

eggNOGiENOG4105BZU. Bacteria.
ENOG410XNMH. LUCA.
HOGENOMiHOG000008986.
KOiK07683.
OMAiRCEGYSN.
OrthoDBiEOG69GZGV.

Enzyme and pathway databases

BioCyciSHAE279808:GJX7-661-MONOMER.

Family and domain databases

Gene3Di3.30.565.10. 1 hit.
InterProiIPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR004358. Sig_transdc_His_kin-like_C.
IPR011712. Sig_transdc_His_kin_sub3_dim/P.
IPR017203. Sig_transdc_His_kinase_NreB.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
PF07730. HisKA_3. 1 hit.
[Graphical view]
PIRSFiPIRSF037432. STHK_NreB. 1 hit.
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
[Graphical view]
SUPFAMiSSF55874. SSF55874. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Whole-genome sequencing of Staphylococcus haemolyticus uncovers the extreme plasticity of its genome and the evolution of human-colonizing staphylococcal species."
    Takeuchi F., Watanabe S., Baba T., Yuzawa H., Ito T., Morimoto Y., Kuroda M., Cui L., Takahashi M., Ankai A., Baba S., Fukui S., Lee J.C., Hiramatsu K.
    J. Bacteriol. 187:7292-7308(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JCSC1435.

Entry informationi

Entry nameiNREB_STAHJ
AccessioniPrimary (citable) accession number: Q4L8Q7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: August 2, 2005
Last modified: March 16, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.