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Protein

Putative formate dehydrogenase SH0748

Gene

SH0748

Organism
Staphylococcus haemolyticus (strain JCSC1435)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Formate + NAD+ = CO2 + NADH.

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi37Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi48Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi51Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi63Iron-sulfur 1 (2Fe-2S)By similarity1
Metal bindingi95Iron-sulfur 2 (4Fe-4S); via tele nitrogenBy similarity1
Metal bindingi99Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi102Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi109Iron-sulfur 2 (4Fe-4S)By similarity1
Metal bindingi147Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi150Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi153Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi157Iron-sulfur 4 (4Fe-4S)By similarity1
Metal bindingi190Iron-sulfur 4 (4Fe-4S)By similarity1
Metal bindingi193Iron-sulfur 4 (4Fe-4S)By similarity1
Metal bindingi196Iron-sulfur 4 (4Fe-4S)By similarity1
Metal bindingi200Iron-sulfur 3 (4Fe-4S)By similarity1
Metal bindingi264Iron-sulfur 5 (4Fe-4S)By similarity1
Metal bindingi267Iron-sulfur 5 (4Fe-4S)By similarity1
Metal bindingi271Iron-sulfur 5 (4Fe-4S)By similarity1
Metal bindingi299Iron-sulfur 5 (4Fe-4S)By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum, NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Putative formate dehydrogenase SH0748 (EC:1.2.1.2)
Gene namesi
Ordered Locus Names:SH0748
OrganismiStaphylococcus haemolyticus (strain JCSC1435)
Taxonomic identifieri279808 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000000543 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003041361 – 984Putative formate dehydrogenase SH0748Add BLAST984

Interactioni

Protein-protein interaction databases

STRINGi279808.SH0748.

Structurei

3D structure databases

ProteinModelPortaliQ4L8G8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 792Fe-2S ferredoxin-typePROSITE-ProRule annotationAdd BLAST77
Domaini138 – 1654Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd BLAST28
Domaini181 – 2114Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd BLAST31
Domaini257 – 3134Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd BLAST57

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni252 – 984Formate dehydrogenaseAdd BLAST733

Sequence similaritiesi

In the C-terminal section; belongs to the prokaryotic molybdopterin-containing oxidoreductase family.Curated
Contains 1 2Fe-2S ferredoxin-type domain.PROSITE-ProRule annotation
Contains 2 4Fe-4S ferredoxin-type domains.PROSITE-ProRule annotation
Contains 1 4Fe-4S Mo/W bis-MGD-type domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4108JB9. Bacteria.
COG3383. LUCA.
HOGENOMiHOG000031440.
KOiK00123.
OMAiWGVTLQP.
OrthoDBiPOG091H037M.

Family and domain databases

CDDicd00207. fer2. 1 hit.
InterProiIPR001041. 2Fe-2S_ferredoxin-type.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR009010. Asp_de-COase-like_dom.
IPR006478. Formate_DH_asu.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR006655. Mopterin_OxRdtase_prok_CS.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd.
[Graphical view]
PfamiPF12838. Fer4_7. 1 hit.
PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
PF10588. NADH-G_4Fe-4S_3. 1 hit.
[Graphical view]
PIRSFiPIRSF036643. FDH_alpha. 1 hit.
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
SM00929. NADH-G_4Fe-4S_3. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
SSF54292. SSF54292. 1 hit.
TIGRFAMsiTIGR01591. Fdh-alpha. 1 hit.
PROSITEiPS51085. 2FE2S_FER_2. 1 hit.
PS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 2 hits.
PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS00932. MOLYBDOPTERIN_PROK_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4L8G8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQEHLIVTLD GTDYLVEPGT SLLEFIKSRD TFVPSICYNE SMGPIQTCDT
60 70 80 90 100
CMVEIDGKIE RACSTVVNRP MTVNTQNNDV KASQKEALDR ILEKHMLYCT
110 120 130 140 150
VCDYNNGDCE IHNAMDAWGL EEQSYEYKTK PYEKDYGPFY RYDPDQCILC
160 170 180 190 200
GRCVEACQDI EVNETISIDW NREHPRVIWD NDVPINESSC VSCGQCATVC
210 220 230 240 250
PCNAMMEVNM EGNAGYMTDT EPGSLAAMID LTKKAEPGDG LLFAVSDSEA
260 270 280 290 300
EMRKERIKKT KTVCTYCGVG CSFDVWTKDR EVLKVQPSHD SPANKIATCV
310 320 330 340 350
KGKFSWGHIN SDQRLTKPLV RKDGEFHEVE WDEALDVIET NFKRIKNEYG
360 370 380 390 400
GDHLAFIASS KGTNEESYLM QKLSRQVFGS NNVDNCSRYC QAPATKGLFR
410 420 430 440 450
TVGHGGDSGS IEDLERAAMT VLIGTNTAEA HPVIASRMKR AQKLFGQKMH
460 470 480 490 500
VFDIRKHEMA ARADAFYQPK PGTDLVWLGA ATKYIIDNDL HDKAFLNDWV
510 520 530 540 550
DNYEDYYKSL ELFTMDFAEE TTGIPKEQII SFAEEAAKAE SMSICWAMGV
560 570 580 590 600
TQQDIGSDTS TAISNLLLVT GNYRKPGSGA YPLRGHNNVQ GASDMGSMPD
610 620 630 640 650
QFPGYQKVAD DEVRAKFEKE YGVELNPTPG RDNHQMMEGI HNGDIQSLYL
660 670 680 690 700
YGEDTGIVDS NINFVQSALE KVDFLVVQDE FLTFTATYAD VVLPASPSLE
710 720 730 740 750
KDGTFTNTER RIQRINKALQ PLGDSKPDWE IFQLIAQRMG ADWNYKHPSE
760 770 780 790 800
IMDEIAGLTP SYSGVNYERL QGFNSLQWPV APDGTDQPTL YMDGFNFENG
810 820 830 840 850
RAKLFPLTFD NFFKEDEVYD LHVNNGRLLE HFHEGNMTYQ TEMIKYKVPN
860 870 880 890 900
AFVEISPELA KDRDIHEGAE LRLISETGEA TLIATVTDRV KGREIYIPLN
910 920 930 940 950
NDAMSNGDLG AINKLTNSDV DKYTDTPSYK RTSCRMEVLT RKGKSPLNPT
960 970 980
NFRVDKQRNP QYSVQVQKKW ERPDYVFPGN VVDK
Length:984
Mass (Da):110,941
Last modified:August 2, 2005 - v1
Checksum:iF0CCF0467C86D89F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006716 Genomic DNA. Translation: BAE04057.1.
RefSeqiWP_011275072.1. NC_007168.1.

Genome annotation databases

EnsemblBacteriaiBAE04057; BAE04057; SH0748.
GeneIDi24248206.
KEGGisha:SH0748.
PATRICi19617516. VBIStaHae67511_0734.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006716 Genomic DNA. Translation: BAE04057.1.
RefSeqiWP_011275072.1. NC_007168.1.

3D structure databases

ProteinModelPortaliQ4L8G8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi279808.SH0748.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAE04057; BAE04057; SH0748.
GeneIDi24248206.
KEGGisha:SH0748.
PATRICi19617516. VBIStaHae67511_0734.

Phylogenomic databases

eggNOGiENOG4108JB9. Bacteria.
COG3383. LUCA.
HOGENOMiHOG000031440.
KOiK00123.
OMAiWGVTLQP.
OrthoDBiPOG091H037M.

Family and domain databases

CDDicd00207. fer2. 1 hit.
InterProiIPR001041. 2Fe-2S_ferredoxin-type.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR009010. Asp_de-COase-like_dom.
IPR006478. Formate_DH_asu.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR006655. Mopterin_OxRdtase_prok_CS.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd.
[Graphical view]
PfamiPF12838. Fer4_7. 1 hit.
PF04879. Molybdop_Fe4S4. 1 hit.
PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
PF10588. NADH-G_4Fe-4S_3. 1 hit.
[Graphical view]
PIRSFiPIRSF036643. FDH_alpha. 1 hit.
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
SM00929. NADH-G_4Fe-4S_3. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
SSF54292. SSF54292. 1 hit.
TIGRFAMsiTIGR01591. Fdh-alpha. 1 hit.
PROSITEiPS51085. 2FE2S_FER_2. 1 hit.
PS00198. 4FE4S_FER_1. 1 hit.
PS51379. 4FE4S_FER_2. 2 hits.
PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS00932. MOLYBDOPTERIN_PROK_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFDHL_STAHJ
AccessioniPrimary (citable) accession number: Q4L8G8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: August 2, 2005
Last modified: November 2, 2016
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.