Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q4L8B8

- TOP3_STAHJ

UniProt

Q4L8B8 - TOP3_STAHJ

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

DNA topoisomerase 3

Gene

topB

Organism
Staphylococcus haemolyticus (strain JCSC1435)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.UniRule annotation

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi8 – 81Magnesium; catalyticUniRule annotation
Sitei60 – 601Interaction with DNAUniRule annotation
Metal bindingi104 – 1041Magnesium; catalyticUniRule annotation
Sitei167 – 1671Interaction with DNAUniRule annotation
Sitei175 – 1751Interaction with DNAUniRule annotation
Active sitei305 – 3051O-(5'-phospho-DNA)-tyrosine intermediateUniRule annotation
Sitei307 – 3071Interaction with DNAUniRule annotation

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. DNA topoisomerase type I activity Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. DNA topological change Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding, Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciSHAE279808:GJX7-800-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 3UniRule annotation (EC:5.99.1.2UniRule annotation)
Alternative name(s):
DNA topoisomerase IIIUniRule annotation
Gene namesi
Name:topBUniRule annotation
Ordered Locus Names:SH0798
OrganismiStaphylococcus haemolyticus (strain JCSC1435)
Taxonomic identifieri279808 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
ProteomesiUP000000543: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 711711DNA topoisomerase 3PRO_0000286378Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi279808.SH0798.

Structurei

3D structure databases

ProteinModelPortaliQ4L8B8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 135134ToprimUniRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni186 – 1916Interaction with DNAUniRule annotation

Sequence similaritiesi

Belongs to the type IA topoisomerase family.UniRule annotation
Contains 1 Toprim domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0551.
HOGENOMiHOG000086847.
KOiK03169.
OMAiTKHDEYF.
OrthoDBiEOG60CWM6.

Family and domain databases

Gene3Di1.10.460.10. 2 hits.
2.70.20.10. 3 hits.
3.40.50.140. 1 hit.
HAMAPiMF_00953. Topoisom_3_prok.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR005738. TopoIII.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
TIGRFAMsiTIGR01056. topB. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q4L8B8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKSLILAEKP SVARDIAEAM NIKGKRNGYI ENEKYVVTWA LGHLVTNAQP
60 70 80 90 100
EHYDKAYKEW KLEDLPIIPK RMQTVVIGKT SKQFKTVKSL ILDKKVKEVI
110 120 130 140 150
IATDAGREGE LVARLILDKV HNKKPIKRLW ISSVTKKAIQ EGFKKLKDGR
160 170 180 190 200
EFQHLYEAAL ARSEADWIVG INATRALTTK YDAQLSLGRV QTPTIQLVNA
210 220 230 240 250
RQQEINHFKA KKYYTLSTEI GGLTFQLSTN KQHMTMEDAT QIANEIKHVE
260 270 280 290 300
GNVDSVEKKV KKSHPKPLYN LTDLQQEAYQ RYKMGPKETL NTIQNLYERH
310 320 330 340 350
KVLTYPRTDS NYLTDDMVDT IKERLYALLA TDYKSQVKSL LGQSYSSKMR
360 370 380 390 400
IFKNHKVSDH HAIIPTEVRP DMQSLSNRES KIYMMVAERF LESLMAPHEY
410 420 430 440 450
EAVRVNVTVG QHIFAFNEKV TRQLGYKALK MNNDNVVKKV AFQKGEKYHL
460 470 480 490 500
QSLKVNEHET TPPDYFNEGS LLKAMENPQN YIQLKEKKHA NTLRQTGGIG
510 520 530 540 550
TVATRADIIE KLFNLNAIES RDGKIKVTSK GKQILDLAPQ ELTSPLLTAE
560 570 580 590 600
WEEKLLLIEK GRYNSRHFID EMKAFTQSIV NTIKNSEQKY KHDNLTTTEC
610 620 630 640 650
PTCGKFMIKV KTKNGQMLVC QDPTCKTKKN VQRKTNARCP NCKKKMTLFG
660 670 680 690 700
RGKDAVYRCV CGHTETQEQM DKRFKNKSSG KVSKKEMKKY MNNEDSLENN
710
PFKDALKNLK L
Length:711
Mass (Da):81,732
Last modified:August 2, 2005 - v1
Checksum:i20D6F54E0A534035
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006716 Genomic DNA. Translation: BAE04107.1.
RefSeqiYP_252713.1. NC_007168.1.

Genome annotation databases

EnsemblBacteriaiBAE04107; BAE04107; SH0798.
GeneIDi3481648.
KEGGisha:SH0798.
PATRICi19617616. VBIStaHae67511_0784.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006716 Genomic DNA. Translation: BAE04107.1 .
RefSeqi YP_252713.1. NC_007168.1.

3D structure databases

ProteinModelPortali Q4L8B8.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 279808.SH0798.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAE04107 ; BAE04107 ; SH0798 .
GeneIDi 3481648.
KEGGi sha:SH0798.
PATRICi 19617616. VBIStaHae67511_0784.

Phylogenomic databases

eggNOGi COG0551.
HOGENOMi HOG000086847.
KOi K03169.
OMAi TKHDEYF.
OrthoDBi EOG60CWM6.

Enzyme and pathway databases

BioCyci SHAE279808:GJX7-800-MONOMER.

Family and domain databases

Gene3Di 1.10.460.10. 2 hits.
2.70.20.10. 3 hits.
3.40.50.140. 1 hit.
HAMAPi MF_00953. Topoisom_3_prok.
InterProi IPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR005738. TopoIII.
IPR006171. Toprim_domain.
[Graphical view ]
PANTHERi PTHR11390. PTHR11390. 1 hit.
Pfami PF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view ]
PRINTSi PR00417. PRTPISMRASEI.
SMARTi SM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view ]
SUPFAMi SSF56712. SSF56712. 1 hit.
TIGRFAMsi TIGR01056. topB. 1 hit.
PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Whole-genome sequencing of Staphylococcus haemolyticus uncovers the extreme plasticity of its genome and the evolution of human-colonizing staphylococcal species."
    Takeuchi F., Watanabe S., Baba T., Yuzawa H., Ito T., Morimoto Y., Kuroda M., Cui L., Takahashi M., Ankai A., Baba S., Fukui S., Lee J.C., Hiramatsu K.
    J. Bacteriol. 187:7292-7308(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JCSC1435.

Entry informationi

Entry nameiTOP3_STAHJ
AccessioniPrimary (citable) accession number: Q4L8B8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: August 2, 2005
Last modified: November 26, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3