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Q4L8B8 (TOP3_STAHJ) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
DNA topoisomerase 3

EC=5.99.1.2
Alternative name(s):
DNA topoisomerase III
Gene names
Name:topB
Ordered Locus Names:SH0798
OrganismStaphylococcus haemolyticus (strain JCSC1435) [Complete proteome] [HAMAP]
Taxonomic identifier279808 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesStaphylococcus

Protein attributes

Sequence length711 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. HAMAP-Rule MF_00953

Catalytic activity

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. HAMAP-Rule MF_00953

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00953

Sequence similarities

Belongs to the type IA topoisomerase family.

Contains 1 Toprim domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 711711DNA topoisomerase 3 HAMAP-Rule MF_00953
PRO_0000286378

Regions

Domain2 – 135134Toprim
Region186 – 1916Interaction with DNA By similarity

Sites

Active site3051O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Metal binding81Magnesium; catalytic By similarity
Metal binding1041Magnesium; catalytic By similarity
Site601Interaction with DNA By similarity
Site1671Interaction with DNA By similarity
Site1751Interaction with DNA By similarity
Site3071Interaction with DNA By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4L8B8 [UniParc].

Last modified August 2, 2005. Version 1.
Checksum: 20D6F54E0A534035

FASTA71181,732
        10         20         30         40         50         60 
MKSLILAEKP SVARDIAEAM NIKGKRNGYI ENEKYVVTWA LGHLVTNAQP EHYDKAYKEW 

        70         80         90        100        110        120 
KLEDLPIIPK RMQTVVIGKT SKQFKTVKSL ILDKKVKEVI IATDAGREGE LVARLILDKV 

       130        140        150        160        170        180 
HNKKPIKRLW ISSVTKKAIQ EGFKKLKDGR EFQHLYEAAL ARSEADWIVG INATRALTTK 

       190        200        210        220        230        240 
YDAQLSLGRV QTPTIQLVNA RQQEINHFKA KKYYTLSTEI GGLTFQLSTN KQHMTMEDAT 

       250        260        270        280        290        300 
QIANEIKHVE GNVDSVEKKV KKSHPKPLYN LTDLQQEAYQ RYKMGPKETL NTIQNLYERH 

       310        320        330        340        350        360 
KVLTYPRTDS NYLTDDMVDT IKERLYALLA TDYKSQVKSL LGQSYSSKMR IFKNHKVSDH 

       370        380        390        400        410        420 
HAIIPTEVRP DMQSLSNRES KIYMMVAERF LESLMAPHEY EAVRVNVTVG QHIFAFNEKV 

       430        440        450        460        470        480 
TRQLGYKALK MNNDNVVKKV AFQKGEKYHL QSLKVNEHET TPPDYFNEGS LLKAMENPQN 

       490        500        510        520        530        540 
YIQLKEKKHA NTLRQTGGIG TVATRADIIE KLFNLNAIES RDGKIKVTSK GKQILDLAPQ 

       550        560        570        580        590        600 
ELTSPLLTAE WEEKLLLIEK GRYNSRHFID EMKAFTQSIV NTIKNSEQKY KHDNLTTTEC 

       610        620        630        640        650        660 
PTCGKFMIKV KTKNGQMLVC QDPTCKTKKN VQRKTNARCP NCKKKMTLFG RGKDAVYRCV 

       670        680        690        700        710 
CGHTETQEQM DKRFKNKSSG KVSKKEMKKY MNNEDSLENN PFKDALKNLK L 

« Hide

References

[1]"Whole-genome sequencing of Staphylococcus haemolyticus uncovers the extreme plasticity of its genome and the evolution of human-colonizing staphylococcal species."
Takeuchi F., Watanabe S., Baba T., Yuzawa H., Ito T., Morimoto Y., Kuroda M., Cui L., Takahashi M., Ankai A., Baba S., Fukui S., Lee J.C., Hiramatsu K.
J. Bacteriol. 187:7292-7308(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JCSC1435.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AP006716 Genomic DNA. Translation: BAE04107.1.
RefSeqYP_252713.1. NC_007168.1.

3D structure databases

ProteinModelPortalQ4L8B8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING279808.SH0798.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAE04107; BAE04107; SH0798.
GeneID3481648.
KEGGsha:SH0798.
PATRIC19617616. VBIStaHae67511_0784.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0551.
HOGENOMHOG000086847.
KOK03169.
OMATKHDEYF.
OrthoDBEOG60CWM6.
ProtClustDBPRK07726.

Enzyme and pathway databases

BioCycSHAE279808:GJX7-800-MONOMER.

Family and domain databases

Gene3D1.10.460.10. 2 hits.
2.70.20.10. 3 hits.
3.40.50.140. 1 hit.
HAMAPMF_00953. Topoisom_3_prok.
InterProIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd.
IPR005738. TopoIII.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERPTHR11390. PTHR11390. 1 hit.
PfamPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSPR00417. PRTPISMRASEI.
SMARTSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMSSF56712. SSF56712. 1 hit.
TIGRFAMsTIGR01056. topB. 1 hit.
PROSITEPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTOP3_STAHJ
AccessionPrimary (citable) accession number: Q4L8B8
Entry history
Integrated into UniProtKB/Swiss-Prot: May 1, 2007
Last sequence update: August 2, 2005
Last modified: February 19, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families