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Protein

PTS system lactose-specific EIICB component

Gene

lacE

Organism
Staphylococcus haemolyticus (strain JCSC1435)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. The enzyme II LacEF PTS system is involved in lactose transport.By similarity

Catalytic activityi

[Protein]-N(pi)-phospho-L-histidine + lactose(Side 1) = [protein]-L-histidine + lactose 6'-phosphate(Side 2).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei487Phosphocysteine intermediate; for EIIB activityBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processPhosphotransferase system, Sugar transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
PTS system lactose-specific EIICB componentBy similarity
Alternative name(s):
EIICB-LacBy similarity
Short name:
EII-LacBy similarity
Including the following 2 domains:
PTS system lactose-specific EIIC componentBy similarity
Alternative name(s):
Lactose permease IIC componentBy similarity
PTS system lactose-specific EIIB componentBy similarity (EC:2.7.1.207By similarity)
Alternative name(s):
Lactose-specific phosphotransferase enzyme IIB componentBy similarity
Gene namesi
Name:lacEBy similarity
Ordered Locus Names:SH0847
OrganismiStaphylococcus haemolyticus (strain JCSC1435)
Taxonomic identifieri279808 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000000543 Componenti: Chromosome

Subcellular locationi

  • Cell membrane PROSITE-ProRule annotationBy similarity; Multi-pass membrane protein PROSITE-ProRule annotationBy similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei30 – 50HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei64 – 84HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei103 – 123HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei137 – 157HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei176 – 196HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei222 – 242HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei283 – 303HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei339 – 359HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei381 – 401HelicalPROSITE-ProRule annotationAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001865941 – 583PTS system lactose-specific EIICB componentAdd BLAST583

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei487Phosphocysteine; by EIIAPROSITE-ProRule annotationBy similarity1

Keywords - PTMi

Phosphoprotein

Expressioni

Inductioni

Induced by lactose, galactose and galactose-6-P. Repressed by glucose.By similarity

Interactioni

Protein-protein interaction databases

STRINGi279808.SH0847.

Structurei

3D structure databases

ProteinModelPortaliQ4L869.
SMRiQ4L869.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini8 – 409PTS EIIC type-3PROSITE-ProRule annotationAdd BLAST402
Domaini480 – 583PTS EIIB type-3PROSITE-ProRule annotationAdd BLAST104

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi456 – 459Poly-ThrSequence analysis4

Domaini

The EIIC type-3 domain forms the PTS system translocation channel and contains the specific substrate-binding site.PROSITE-ProRule annotation
The PTS EIIB type-3 domain is phosphorylated by phospho-EIIA on a cysteinyl residue. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the PTS EIIC type-3 domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CK1. Bacteria.
COG1455. LUCA.
HOGENOMiHOG000235576.
KOiK02787.
K02788.
OMAiISAMPFM.
OrthoDBiPOG091H06IM.

Family and domain databases

InterProiView protein in InterPro
IPR036095. PTS_EIIB-like_sf.
IPR003501. PTS_EIIB_2/3.
IPR013012. PTS_EIIB_3.
IPR003352. PTS_EIIC.
IPR004501. PTS_EIIC_3.
IPR004801. PTS_IIC_lac_spec.
PfamiView protein in Pfam
PF02378. PTS_EIIC. 1 hit.
PF02302. PTS_IIB. 1 hit.
SUPFAMiSSF52794. SSF52794. 1 hit.
TIGRFAMsiTIGR00394. lac_pts_IIC. 1 hit.
TIGR00410. lacE. 1 hit.
TIGR00853. pts-lac. 1 hit.
PROSITEiView protein in PROSITE
PS51100. PTS_EIIB_TYPE_3. 1 hit.
PS51105. PTS_EIIC_TYPE_3. 1 hit.

Sequencei

Sequence statusi: Complete.

Q4L869-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKLIAWIEK GKPFFEKISR NIYLRAIRDG FIAAIPIILF SSIFILITYV
60 70 80 90 100
PNVFGFTWSK TMEGILMKPY NYTMGIVGLI VAGTTAKSLT DSYNRKLDKT
110 120 130 140 150
NQINFISTMM AAMSGFLFLA ADPIKEGGFL SAFMGTKGLL TAFISAFITV
160 170 180 190 200
IVYNFFIKRN ITIKMPKEVP PNISQVFKDI FPLSAVIIII YALDLLSRTF
210 220 230 240 250
IHTNVANAVL KIFEPLFTAA DGWIGVTLIF GAFAFFWFVG IHGPSIVEPA
260 270 280 290 300
IAAITYANLE TNLNLIQAGE HADKIITPGT QMFVATMGGT GATLVVPFMF
310 320 330 340 350
MWLTKSKRNK AIGRASVVPT FFGVNEPILF GAPLVLNPVF FIPFIFAPIV
360 370 380 390 400
NVWIFKFFVD VLKMNSFSIF LPWTTPGPLG IVMGTGFAFW SFVLAIVLIV
410 420 430 440 450
VDVMIYYPFL KVYDEQILEE ERGNKEVNNE LKEKVSANFD TKKADAILAT
460 470 480 490 500
AGANETTTTE SAPSDEEVSA KNSSNSINEQ TNVLVLCAGG GTSGLLANAL
510 520 530 540 550
NKAAEEYQVP VKAAAGGYGA HMDIMKDYQL IILAPQVASN YEDIKQDTDR
560 570 580
LGIKLAKTQG VEYINLTRDG KAALDFVQQQ FEK
Length:583
Mass (Da):64,211
Last modified:August 2, 2005 - v1
Checksum:iDCBDAECEF9CB5654
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006716 Genomic DNA. Translation: BAE04156.1.
RefSeqiWP_011275159.1. NC_007168.1.

Genome annotation databases

EnsemblBacteriaiBAE04156; BAE04156; SH0847.
GeneIDi24248372.
KEGGisha:SH0847.

Similar proteinsi

Entry informationi

Entry nameiPTLCB_STAHJ
AccessioniPrimary (citable) accession number: Q4L869
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: August 2, 2005
Last modified: November 22, 2017
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome