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Q4L869 (PTLCB_STAHJ) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 50. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
PTS system lactose-specific EIICB component
Alternative name(s):
EIICB-Lac
Short name=EII-Lac

Including the following 2 domains:

  1. Lactose permease IIC component
    Alternative name(s):
    PTS system lactose-specific EIIC component
  2. Lactose-specific phosphotransferase enzyme IIB component
    EC=2.7.1.69
    Alternative name(s):
    PTS system lactose-specific EIIB component
Gene names
Name:lacE
Ordered Locus Names:SH0847
OrganismStaphylococcus haemolyticus (strain JCSC1435) [Complete proteome] [HAMAP]
Taxonomic identifier279808 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length583 AA.
Sequence statusComplete.
Protein existencePredicted

General annotation (Comments)

Function

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in lactose transport.

Catalytic activity

Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.

Subcellular location

Cell membrane; Multi-pass membrane protein Probable.

Domain

The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.

The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.

Sequence similarities

Contains 1 PTS EIIB type-3 domain.

Contains 1 PTS EIIC type-3 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 583583PTS system lactose-specific EIICB component
PRO_0000186594

Regions

Transmembrane29 – 4921Helical; Potential
Transmembrane60 – 8021Helical; Potential
Transmembrane101 – 12121Helical; Potential
Transmembrane130 – 15021Helical; Potential
Transmembrane173 – 19321Helical; Potential
Transmembrane218 – 23821Helical; Potential
Transmembrane277 – 29721Helical; Potential
Transmembrane333 – 35321Helical; Potential
Transmembrane374 – 39421Helical; Potential
Domain480 – 583104PTS EIIB type-3

Sites

Active site4871Phosphocysteine intermediate; for EIIB activity By similarity

Sequences

Sequence LengthMass (Da)Tools
Q4L869 [UniParc].

Last modified August 2, 2005. Version 1.
Checksum: DCBDAECEF9CB5654

FASTA58364,211
        10         20         30         40         50         60 
MNKLIAWIEK GKPFFEKISR NIYLRAIRDG FIAAIPIILF SSIFILITYV PNVFGFTWSK 

        70         80         90        100        110        120 
TMEGILMKPY NYTMGIVGLI VAGTTAKSLT DSYNRKLDKT NQINFISTMM AAMSGFLFLA 

       130        140        150        160        170        180 
ADPIKEGGFL SAFMGTKGLL TAFISAFITV IVYNFFIKRN ITIKMPKEVP PNISQVFKDI 

       190        200        210        220        230        240 
FPLSAVIIII YALDLLSRTF IHTNVANAVL KIFEPLFTAA DGWIGVTLIF GAFAFFWFVG 

       250        260        270        280        290        300 
IHGPSIVEPA IAAITYANLE TNLNLIQAGE HADKIITPGT QMFVATMGGT GATLVVPFMF 

       310        320        330        340        350        360 
MWLTKSKRNK AIGRASVVPT FFGVNEPILF GAPLVLNPVF FIPFIFAPIV NVWIFKFFVD 

       370        380        390        400        410        420 
VLKMNSFSIF LPWTTPGPLG IVMGTGFAFW SFVLAIVLIV VDVMIYYPFL KVYDEQILEE 

       430        440        450        460        470        480 
ERGNKEVNNE LKEKVSANFD TKKADAILAT AGANETTTTE SAPSDEEVSA KNSSNSINEQ 

       490        500        510        520        530        540 
TNVLVLCAGG GTSGLLANAL NKAAEEYQVP VKAAAGGYGA HMDIMKDYQL IILAPQVASN 

       550        560        570        580 
YEDIKQDTDR LGIKLAKTQG VEYINLTRDG KAALDFVQQQ FEK

« Hide

References

[1]"Whole-genome sequencing of Staphylococcus haemolyticus uncovers the extreme plasticity of its genome and the evolution of human-colonizing staphylococcal species."
Takeuchi F., Watanabe S., Baba T., Yuzawa H., Ito T., Morimoto Y., Kuroda M., Cui L., Takahashi M., Ankai A., Baba S., Fukui S., Lee J.C., Hiramatsu K.
J. Bacteriol. 187:7292-7308(2005) [PubMed: 16237012] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JCSC1435.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AP006716 Genomic DNA. Translation: BAE04156.1.
RefSeqYP_252762.1. NC_007168.1.

3D structure databases

ProteinModelPortalQ4L869.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ4L869.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaEBSTAT00000044993; EBSTAP00000043444; EBSTAG00000044990.
GeneID3482144.
GenomeReviewsGene locus SH0847 in contig AP006716_GR.
KEGGsha:SH0847.
NMPDRfig|279808.3.peg.860.
PATRIC19617712. VBIStaHae67511_0832.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1455.
GeneTreeEBGT00050000025328.
HOGENOMHBG337916.
OMAWSKDIEN.
PhylomeDBQ4L869.
ProtClustDBCLSK877288.

Enzyme and pathway databases

BioCycSHAE279808:SH0847-MONOMER.

Family and domain databases

InterProIPR014350. PTrfase_system_EIIB_3_subgr.
IPR003501. PTS_EIIB_2/3.
IPR013012. PTS_EIIB_3.
IPR003352. PTS_EIIC.
IPR004501. PTS_IIC_lac.
IPR004801. PTS_IIC_lac_spec.
[Graphical view]
KOK02787.
K02788.
PfamPF02378. PTS_EIIC. 1 hit.
PF02302. PTS_IIB. 1 hit.
[Graphical view]
TIGRFAMsTIGR00394. Lac_pts_IIC. 1 hit.
TIGR00410. LacE. 1 hit.
TIGR00853. Pts-lac. 1 hit.
PROSITEPS51100. PTS_EIIB_TYPE_3. 1 hit.
PS51105. PTS_EIIC_TYPE_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePTLCB_STAHJ
AccessionPrimary (citable) accession number: Q4L869
Entry history
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: August 2, 2005
Last modified: January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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