Reviewed, UniProtKB/Swiss-Prot Q4L869 (PTLCB_STAHJ)

Names and origin

Protein names

Recommended name:
    PTS system lactose-specific EIICB component
Alternative name(s):
    EIICB-Lac
      Short name=EII-Lac
Including the following 2 domains:
    1- Recommended name:
            Lactose permease IIC component
        Alternative name(s):
            PTS system lactose-specific EIIC component
    2- Recommended name:
            Lactose-specific phosphotransferase enzyme IIB component
              EC=2.7.1.69
        Alternative name(s):
            PTS system lactose-specific EIIB component

Gene names

Name:	lacE
Ordered Locus Names:	SH0847

Organism

Staphylococcus haemolyticus (strain JCSC1435) [Complete proteome] [HAMAP]

Taxonomic identifier

279808 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	583 AA.
Sequence status	Complete.
Protein existence	Predicted.

General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in lactose transport.
Catalytic activity	Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.
Subcellular location	Cell membrane; Multi-pass membrane protein Probable.
Domain	The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site. The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.
Sequence similarities	Contains 1 PTS EIIB type-3 domain. Contains 1 PTS EIIC type-3 domain.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	kinase activity Inferred from electronic annotation. Source: UniProtKB-KW protein-N(PI)-phosphohistidine-sugar phosphotransferase activity Inferred from electronic annotation. Source: EC sugar binding Inferred from electronic annotation. Source: InterPro sugar:hydrogen symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 583	583	PTS system lactose-specific EIICB component		PRO_0000186594
Regions
	Transmembrane	29 – 49	21	Potential
	Transmembrane	60 – 80	21	Potential
	Transmembrane	101 – 121	21	Potential
	Transmembrane	130 – 150	21	Potential
	Transmembrane	173 – 193	21	Potential
	Transmembrane	218 – 238	21	Potential
	Transmembrane	277 – 297	21	Potential
	Transmembrane	333 – 353	21	Potential
	Transmembrane	374 – 394	21	Potential
	Domain	480 – 583	104	PTS EIIB type-3
Sites
	Active site	487	1	Phosphocysteine intermediate; for EIIB activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4L869-1 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: DCBDAECEF9CB5654	FASTA	583	64,211
	10 20 30 40 50 60 MNKLIAWIEK GKPFFEKISR NIYLRAIRDG FIAAIPIILF SSIFILITYV PNVFGFTWSK 70 80 90 100 110 120 TMEGILMKPY NYTMGIVGLI VAGTTAKSLT DSYNRKLDKT NQINFISTMM AAMSGFLFLA 130 140 150 160 170 180 ADPIKEGGFL SAFMGTKGLL TAFISAFITV IVYNFFIKRN ITIKMPKEVP PNISQVFKDI 190 200 210 220 230 240 FPLSAVIIII YALDLLSRTF IHTNVANAVL KIFEPLFTAA DGWIGVTLIF GAFAFFWFVG 250 260 270 280 290 300 IHGPSIVEPA IAAITYANLE TNLNLIQAGE HADKIITPGT QMFVATMGGT GATLVVPFMF 310 320 330 340 350 360 MWLTKSKRNK AIGRASVVPT FFGVNEPILF GAPLVLNPVF FIPFIFAPIV NVWIFKFFVD 370 380 390 400 410 420 VLKMNSFSIF LPWTTPGPLG IVMGTGFAFW SFVLAIVLIV VDVMIYYPFL KVYDEQILEE 430 440 450 460 470 480 ERGNKEVNNE LKEKVSANFD TKKADAILAT AGANETTTTE SAPSDEEVSA KNSSNSINEQ 490 500 510 520 530 540 TNVLVLCAGG GTSGLLANAL NKAAEEYQVP VKAAAGGYGA HMDIMKDYQL IILAPQVASN 550 560 570 580 YEDIKQDTDR LGIKLAKTQG VEYINLTRDG KAALDFVQQQ FEK

References

[1]

"Whole-genome sequencing of Staphylococcus haemolyticus uncovers the extreme plasticity of its genome and the evolution of human-colonizing staphylococcal species." Takeuchi F., Watanabe S., Baba T., Yuzawa H., Ito T., Morimoto Y., Kuroda M., Cui L., Takahashi M., Ankai A., Baba S., Fukui S., Lee J.C., Hiramatsu K. J. Bacteriol. 187:7292-7308(2005) [PubMed: 16237012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006716 Genomic DNA. Translation: BAE04156.1.
RefSeq	YP_252762.1.
3D structure databases
SMR	Q4L869. Positions 479-581.
ModBase	Search...
Protein-protein interaction databases
STRING	Q4L869.
Genome annotation databases
GeneID	3482144.
GenomeReviews	Gene locus SH0847 in contig AP006716_GR.
KEGG	sha:SH0847.
NMPDR	fig|279808.3.peg.860.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1455.
HOGENOM	HBG337916.
OMA	WSKDIEN.
PhylomeDB	Q4L869.
Enzyme and pathway databases
BioCyc	SHAE279808:SH0847-MONOMER.
Family and domain databases
InterPro	IPR014350. PTrfase_system_EIIB_3_subgr. IPR003501. PTS_EIIB_2/3. IPR013012. PTS_EIIB_3. IPR003352. PTS_EIIC. IPR004501. PTS_IIC_lac. IPR004801. PTS_IIC_lac_spec. [Graphical view]
Pfam	PF02378. PTS_EIIC. 1 hit. PF02302. PTS_IIB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00394. lac_pts_IIC. 1 hit. TIGR00410. lacE. 1 hit. TIGR00853. pts-lac. 1 hit.
PROSITE	PS51100. PTS_EIIB_TYPE_3. 1 hit. PS51105. PTS_EIIC_TYPE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTLCB_STAHJ

Accession

Primary (citable) accession number: Q4L869

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	August 2, 2005
Last modified:	February 9, 2010
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)