Q4L868 (LACG_STAHJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
6-phospho-beta-galactosidase
EC=3.2.1.85

Alternative name(s):
Beta-D-phosphogalactoside galactohydrolase
Short name=PGALase
P-beta-Gal
Short name=PBG

Gene names

Name:	lacG
Ordered Locus Names:	SH0848

Organism

Staphylococcus haemolyticus (strain JCSC1435) [Complete proteome] [HAMAP]

Taxonomic identifier

279808 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	469 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	A 6-phospho-beta-D-galactoside + H2O = 6-phospho-D-galactose + an alcohol. HAMAP MF_01574
Pathway	Carbohydrate metabolism; lactose degradation; D-galactose 6-phosphate and beta-D-glucose from lactose 6-phosphate: step 1/1. HAMAP MF_01574
Sequence similarities	Belongs to the glycosyl hydrolase 1 family.

Ontologies

Keywords
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lactose catabolic process via tagatose-6-phosphate Inferred from electronic annotation. Source: InterPro
Molecular function	6-phospho-beta-galactosidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 469	469	6-phospho-beta-galactosidase HAMAP MF_01574		PRO_0000260727
Sites
	Active site	159	1	Proton donor By similarity
	Active site	374	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4L868 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: 0D933E94BF925793	FASTA	469	54,646
	10 20 30 40 50 60 MKKLPEDFIF GGATAAYQAE GATQTDGKGR VAWDTYLEEN YWYTAEPASD FYNKYPIDLE 70 80 90 100 110 120 LSEKFGVNGI RISIAWSRIF PKGYGEVNPK GVEYYHNLFK ECHRRHVEPF VTLHHFDTPE 130 140 150 160 170 180 TLHSDGDFLN RKTIEYFVEY AKFCFEEFEE VNYWTTFNEI GPIGDGQYLV GKFPPGIKYD 190 200 210 220 230 240 FAKVFQSHHN MMVAHAKAVK LFKDNGYSGE VGVVHALPTK YPYDPTNPED VRAAELEDII 250 260 270 280 290 300 HNKFILDATY LGKYSRETME GVQHILSVNG GKLDIPEEDY KVLEAAKDLN DFLGINYYMS 310 320 330 340 350 360 DWMRGYDGES EITHNATGDK GGSKYQLKGV GQREFDVDVP RTDWDWMIYP KGLYDQIMRV 370 380 390 400 410 420 VKDYPNYHKI YITENGLGYK DQFDEERKTV DDDARIDYVK KHLEVISDAI RDGANVKGYF 430 440 450 460 IWSLMDVFSW SNGYEKRYGL FYVDFETQER YPKKSAYWYK ELAESKEIK

References

[1]

"Whole-genome sequencing of Staphylococcus haemolyticus uncovers the extreme plasticity of its genome and the evolution of human-colonizing staphylococcal species." Takeuchi F., Watanabe S., Baba T., Yuzawa H., Ito T., Morimoto Y., Kuroda M., Cui L., Takahashi M., Ankai A., Baba S., Fukui S., Lee J.C., Hiramatsu K. J. Bacteriol. 187:7292-7308(2005) [PubMed: 16237012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: JCSC1435.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006716 Genomic DNA. Translation: BAE04157.1.
RefSeq	YP_252763.1. NC_007168.1.
3D structure databases
HSSP	HSSP built from PDB template 1PBG based on UniProtKB P11546.
ProteinModelPortal	Q4L868.
SMR	Q4L868. Positions 2-469.
ModBase	Search...
Protein-protein interaction databases
STRING	Q4L868.
Protein family/group databases
CAZy	GH1. Glycoside Hydrolase Family 1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTAT00000044681; EBSTAP00000043132; EBSTAG00000044679.
GeneID	3482146.
GenomeReviews	Gene locus SH0848 in contig AP006716_GR.
KEGG	sha:SH0848.
NMPDR	fig|279808.3.peg.861.
PATRIC	19617714. VBIStaHae67511_0833.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2723.
GeneTree	EBGT00050000024342.
HOGENOM	HBG316462.
OMA	FVNYAEF.
PhylomeDB	Q4L868.
ProtClustDB	PRK13511.
Enzyme and pathway databases
BioCyc	SHAE279808:SH0848-MONOMER.
Family and domain databases
HAMAP	MF_01574. LacG. [Tree]
InterPro	IPR005928. 6P-beta-galactosidase. IPR001360. Glyco_hydro_1. IPR018120. Glyco_hydro_1_AS. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
KO	K01220.
PANTHER	PTHR10353. Glyco_hydro_1. 1 hit.
Pfam	PF00232. Glyco_hydro_1. 1 hit. [Graphical view]
PRINTS	PR00131. GLHYDRLASE1.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
TIGRFAMs	TIGR01233. LacG. 1 hit.
PROSITE	PS00572. GLYCOSYL_HYDROL_F1_1. 1 hit. PS00653. GLYCOSYL_HYDROL_F1_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LACG_STAHJ

Accession

Primary (citable) accession number: Q4L868

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	August 2, 2005
Last modified:	January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program