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Protein

Hydroxyethylthiazole kinase

Gene

thiM

Organism
Staphylococcus haemolyticus (strain JCSC1435)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).UniRule annotation

Catalytic activityi

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Hydroxyethylthiazole kinase (thiM)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei39Substrate; via amide nitrogenUniRule annotation1
Binding sitei115ATPUniRule annotation1
Binding sitei160ATPUniRule annotation1
Binding sitei187Substrate; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processThiamine biosynthesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSHAE279808:G1G27-905-MONOMER
UniPathwayiUPA00060; UER00139

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyethylthiazole kinaseUniRule annotation (EC:2.7.1.50UniRule annotation)
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinaseUniRule annotation
Short name:
TH kinaseUniRule annotation
Short name:
Thz kinaseUniRule annotation
Gene namesi
Name:thiMUniRule annotation
Ordered Locus Names:SH0942
OrganismiStaphylococcus haemolyticus (strain JCSC1435)
Taxonomic identifieri279808 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000000543 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000215351 – 263Hydroxyethylthiazole kinaseAdd BLAST263

Interactioni

Protein-protein interaction databases

STRINGi279808.SH0942

Structurei

3D structure databases

ProteinModelPortaliQ4L7X4
SMRiQ4L7X4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Thz kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C4U Bacteria
COG2145 LUCA
HOGENOMiHOG000114352
KOiK00878
OMAiKRPLVHN
OrthoDBiPOG091H004S

Family and domain databases

CDDicd01170 THZ_kinase, 1 hit
Gene3Di3.40.1190.20, 1 hit
HAMAPiMF_00228 Thz_kinase, 1 hit
InterProiView protein in InterPro
IPR000417 Hyethyz_kinase
IPR029056 Ribokinase-like
PANTHERiPTHR44423 PTHR44423, 1 hit
PfamiView protein in Pfam
PF02110 HK, 1 hit
PIRSFiPIRSF000513 Thz_kinase, 1 hit
PRINTSiPR01099 HYETHTZKNASE
SUPFAMiSSF53613 SSF53613, 1 hit
TIGRFAMsiTIGR00694 thiM, 1 hit

Sequencei

Sequence statusi: Complete.

Q4L7X4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNLERLRQE NPLVVCYTND VVKNFTANGL LSIGASPAMS EAPEEAKEFY
60 70 80 90 100
KVAGALLINI GTMTKANEQD ILEIGKIANQ QGTPIVFDPV AVGASSYRKA
110 120 130 140 150
FCQKFLSEVK VSVIKGNASE ILTLVDATTT MKGTDGKTDL DVVEIAKRAH
160 170 180 190 200
EELNTAIVLT GKDDVVVQGG KVVKLSNGSP LLAKITGAGC LLGGIVASFL
210 220 230 240 250
FREENPTLQV LEEAVSIYNI AAEIAEKDQQ VNGPGTFLPK LLDQMYNIDF
260
NTYQQQVKRQ EVE
Length:263
Mass (Da):28,438
Last modified:August 2, 2005 - v1
Checksum:i89E09D7A16987BE5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006716 Genomic DNA Translation: BAE04251.1
RefSeqiWP_011275253.1, NC_007168.1

Genome annotation databases

EnsemblBacteriaiBAE04251; BAE04251; SH0942
GeneIDi24246008
KEGGisha:SH0942

Similar proteinsi

Entry informationi

Entry nameiTHIM_STAHJ
AccessioniPrimary (citable) accession number: Q4L7X4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: August 2, 2005
Last modified: April 25, 2018
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health