Reviewed, UniProtKB/Swiss-Prot Q4L7U4 (THD1_STAHJ)

Names and origin

Protein names

Recommended name:
    Threonine dehydratase biosynthetic
    EC=4.3.1.19
Alternative name(s):
    Threonine deaminase

Gene names

Name:	ilvA
Ordered Locus Names:	SH0972

Organism

Staphylococcus haemolyticus (strain JCSC1435) [Complete proteome] [HAMAP]

Taxonomic identifier

279808 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	422 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of alpha-ketobutyrate from threonine in a two-step reaction. The first step is a dehydration of threonine, followed by rehydration and liberation of ammonia By similarity.
Catalytic activity	L-threonine = 2-oxobutanoate + NH3.
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; 2-oxobutanoate from L-threonine: step 1/1.
Sequence similarities	Belongs to the serine/threonine dehydratase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis Isoleucine biosynthesis
Ligand	Pyridoxal phosphate
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	L-threonine ammonia-lyase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 422	422	Threonine dehydratase biosynthetic		PRO_0000234314
Amino acid modifications
	Modified residue	56	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4L7U4-1 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: 6CA7388A563960E0	FASTA	422	46,986
	10 20 30 40 50 60 MTVKTTLNAK DVDNAYLRIK DVVKETPLQF DLYLSQKYDC NVYLKREDLQ WVRSFKLRGA 70 80 90 100 110 120 YNAISVLTSE AKEKGITCAS AGNHAQGVAY TAKALNLKAV IFMPVTTPLQ KVNQVKFFGS 130 140 150 160 170 180 KNVKIILTGD TFDDCLKEAL IYTEQNHMTF IDPFNNVDTI AGQGTLAKEI LNQSSNDSIT 190 200 210 220 230 240 FDYLFAAIGG GGLISGISTY MNQYSPQTKI IGVEPSGASS MYESVVVQNK IVTLDHIDKF 250 260 270 280 290 300 VDGASVARVG DITYDIAKKF VDDYIQVDEG AVCSTILDMY SKQAIVAEPA GALSVSALEQ 310 320 330 340 350 360 YKEKIKGKTV VCVVSGGNND INRMKEIEER SLLYEEMKHY FILNFPQRPG ALKEFVNDVL 370 380 390 400 410 420 GPQDDITKFE YLKKTSQNTG TVIIGIQLKN HDDLNQLKIN VHDFDPSNIY INENKMLYSL LI

References

[1]

"Whole-genome sequencing of Staphylococcus haemolyticus uncovers the extreme plasticity of its genome and the evolution of human-colonizing staphylococcal species." Takeuchi F., Watanabe S., Baba T., Yuzawa H., Ito T., Morimoto Y., Kuroda M., Cui L., Takahashi M., Ankai A., Baba S., Fukui S., Lee J.C., Hiramatsu K. J. Bacteriol. 187:7292-7308(2005) [PubMed: 16237012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP006716 Genomic DNA. Translation: BAE04281.1.
RefSeq	YP_252887.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3482161.
GenomeReviews	Gene locus SH0972 in contig AP006716_GR.
KEGG	sha:SH0972.
NMPDR	fig|279808.3.peg.1404.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q4L7U4.
OMA	Q4L7U4. IFMPTTT.
Enzyme and pathway databases
BioCyc	SHAE279808:SH0972-MON.
Family and domain databases
InterPro	IPR001926. PyrdxlP-dep_enz_bsu. IPR000634. Ser/Thr_deHydtase_PyrdxlP-BS. IPR001721. Thr_deHydtase_C. IPR011820. Threonine_deHydtase. [Graphical view]
Pfam	PF00291. PALP. 1 hit. PF00585. Thr_dehydrat_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR02079. THD1. 1 hit.
PROSITE	PS00165. DEHYDRATASE_SER_THR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

THD1_STAHJ

Accession

Primary (citable) accession number: Q4L7U4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	August 2, 2005
Last modified:	June 16, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)