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Protein

Diacylglycerol kinase

Gene

dagK

Organism
Staphylococcus haemolyticus (strain JCSC1435)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is a key enzyme involved in the production of lipoteichoic acid by reintroducing DAG formed from the breakdown of membrane phospholipids into the phosphatidylglycerol biosynthetic pathway (By similarity).By similarity

Catalytic activityi

ATP + 1,2-diacyl-sn-glycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit. This ion appears to have a structural role and is required for catalytic activity.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei41ATPPROSITE-ProRule annotation1
Binding sitei94ATPPROSITE-ProRule annotation1
Metal bindingi213Magnesium; via carbonyl oxygenBy similarity1
Metal bindingi216MagnesiumBy similarity1
Metal bindingi218Magnesium; via carbonyl oxygenBy similarity1
Active sitei273Proton acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 14ATPPROSITE-ProRule annotation5
Nucleotide bindingi67 – 73ATPPROSITE-ProRule annotation7

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processLipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Diacylglycerol kinase (EC:2.7.1.107)
Short name:
DAG kinase
Short name:
DAGK
Gene namesi
Name:dagK
Ordered Locus Names:SH1055
OrganismiStaphylococcus haemolyticus (strain JCSC1435)
Taxonomic identifieri279808 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000000543 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003865021 – 330Diacylglycerol kinaseAdd BLAST330

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi279808.SH1055

Structurei

3D structure databases

ProteinModelPortaliQ4L7L1
SMRiQ4L7L1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 132DAGKcPROSITE-ProRule annotationAdd BLAST132

Sequence similaritiesi

Belongs to the diacylglycerol/lipid kinase family.Curated

Phylogenomic databases

eggNOGiENOG4105CBD Bacteria
COG1597 LUCA
HOGENOMiHOG000261395
KOiK07029
OMAiEHINDPH
OrthoDBiPOG091H02HT

Family and domain databases

Gene3Di3.40.50.10330, 1 hit
InterProiView protein in InterPro
IPR017438 ATP-NAD_kinase_N
IPR005218 Diacylglycerol/lipid_kinase
IPR001206 Diacylglycerol_kinase_cat_dom
IPR016064 NAD/diacylglycerol_kinase_sf
PfamiView protein in Pfam
PF00781 DAGK_cat, 1 hit
SMARTiView protein in SMART
SM00046 DAGKc, 1 hit
SUPFAMiSSF111331 SSF111331, 1 hit
TIGRFAMsiTIGR00147 TIGR00147, 1 hit
PROSITEiView protein in PROSITE
PS50146 DAGK, 1 hit

Sequencei

Sequence statusi: Complete.

Q4L7L1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKCARIIYN PTSGKELFKR TLPDVLIKLE RAGYETSAYA TEREGDATLE
60 70 80 90 100
AERALKRDYD IIIAAGGDGT LNEVVNGIAE QPNRPKLGII PMGTVNDFGR
110 120 130 140 150
ALHLPSDIMG AVDVIIDDHT TKVDIGKMNN RYFINLAAGG QLTQVSYETP
160 170 180 190 200
SRLKSIVGPF AYYIKGFEML PQMKAVDLRI EYDNQVFQGE ALLFLLGLTN
210 220 230 240 250
SMAGFEKLVP DAKLDDGHFT LIIVEKANLA ELGHIMTLAS RGEHIKHPKV
260 270 280 290 300
IYEKAKSINI SSFTEMQLNV DGEYGGKLPA NFLNLKRHIE VCTPKDIYNE
310 320 330
ELTEDQQVED GIIEEKPSNE EIIKNNEISE
Length:330
Mass (Da):36,803
Last modified:August 2, 2005 - v1
Checksum:i218DD852D68697D1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006716 Genomic DNA Translation: BAE04364.1
RefSeqiWP_011275360.1, NC_007168.1

Genome annotation databases

EnsemblBacteriaiBAE04364; BAE04364; SH1055
GeneIDi24247529
KEGGisha:SH1055

Similar proteinsi

Entry informationi

Entry nameiDAGK_STAHJ
AccessioniPrimary (citable) accession number: Q4L7L1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 13, 2009
Last sequence update: August 2, 2005
Last modified: April 25, 2018
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health