Reviewed, UniProtKB/Swiss-Prot Q4L750 (DHA_STAHJ)

Names and origin

Protein names

Recommended name:
    Alanine dehydrogenase
    EC=1.4.1.1

Gene names

Name:	ald
Ordered Locus Names:	SH1216

Organism

Staphylococcus haemolyticus (strain JCSC1435) [Complete proteome] [HAMAP]

Taxonomic identifier

279808 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	373 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	May play a role in cell wall synthesis as L-alanine is an important constituent of the peptidoglycan layer By similarity.
Catalytic activity	L-alanine + H2O + NAD+ = pyruvate + NH3 + NADH.
Pathway	Amino-acid degradation; L-alanine degradation via dehydrogenase pathway; NH(3) and pyruvate from L-alanine: step 1/1.
Sequence similarities	Belongs to the AlaDH/PNT family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alanine dehydrogenase activity Inferred from electronic annotation. Source: EC binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 373	373	Alanine dehydrogenase		PRO_0000287324
Regions
	Nucleotide binding	169 – 199	31	NAD By similarity
Sites
	Active site	95	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q4L750-1 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: 66E91EE689108294	FASTA	373	39,966
	10 20 30 40 50 60 MKIGIPKEIK NNENRVGLSP SGVHALVEQG HTVLVEKDAG LGSFFEDKDY KDAGADIVSE 70 80 90 100 110 120 QSSVWDVEMV IKVKEPLEEE YKYFKEGLIL FTYLHLANEE KLTQALVDNK VVGIAYETVQ 130 140 150 160 170 180 LPDRSLPLLT PMSEVAGRMS AQVGSQFLQK FNGGMGILLG GVPGVPKGKV SIIGGGQAGT 190 200 210 220 230 240 NAAKIALGLG ANVTILDVNP KRLAELDDLF DGRVNTIMSN PLNIENAVKE SDLVIGAVLI 250 260 270 280 290 300 PGAKAPSLVT EDMIKQMKDG SVIVDIAIDQ GGIFETTDKI TTHDDPTYVK HGVVHYAVAN 310 320 330 340 350 360 MPGAVPRTST IALNNATLPY AQLLASKGYR EAFKANHALS LGLNTYKGHV THKGVAEAFG 370 LEYTSVEDAL KED

References

[1]

"Whole-genome sequencing of Staphylococcus haemolyticus uncovers the extreme plasticity of its genome and the evolution of human-colonizing staphylococcal species." Takeuchi F., Watanabe S., Baba T., Yuzawa H., Ito T., Morimoto Y., Kuroda M., Cui L., Takahashi M., Ankai A., Baba S., Fukui S., Lee J.C., Hiramatsu K. J. Bacteriol. 187:7292-7308(2005) [PubMed: 16237012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AP006716 Genomic DNA. Translation: BAE04525.1.
RefSeq	YP_253131.1.
3D structure databases
SMR	Q4L750. Positions 1-371.
ModBase	Search...
Protein-protein interaction databases
STRING	Q4L750.
Genome annotation databases
GeneID	3482407.
GenomeReviews	Gene locus SH1216 in contig AP006716_GR.
KEGG	sha:SH1216.
NMPDR	fig|279808.3.peg.2455.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0686.
HOGENOM	HBG289735.
OMA	VAHGHEV.
PhylomeDB	Q4L750.
Enzyme and pathway databases
BioCyc	SHAE279808:SH1216-MONOMER.
Family and domain databases
InterPro	IPR007698. Ala_DH/PNT_C. IPR008142. Ala_DH/PNT_CS1. IPR008143. Ala_DH/PNT_CS2. IPR007886. Ala_DH/PNT_N. IPR008141. Ala_DH/Pyr_transHydgase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF01262. AlaDh_PNT_C. 1 hit. PF05222. AlaDh_PNT_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00518. alaDH. 1 hit.
PROSITE	PS00836. ALADH_PNT_1. 1 hit. PS00837. ALADH_PNT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHA_STAHJ

Accession

Primary (citable) accession number: Q4L750

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	August 2, 2005
Last modified:	February 9, 2010
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)