ID   KPYK_STAHJ              Reviewed;         586 AA.
AC   Q4L739;
DT   10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT   02-AUG-2005, sequence version 1.
DT   16-JUN-2009, entry version 27.
DE   RecName: Full=Pyruvate kinase;
DE            Short=PK;
DE            EC=2.7.1.40;
GN   Name=pyk; OrderedLocusNames=SH1227;
OS   Staphylococcus haemolyticus (strain JCSC1435).
OC   Bacteria; Firmicutes; Bacillales; Staphylococcus.
OX   NCBI_TaxID=279808;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16237012; DOI=10.1128/JB.187.21.7292-7308.2005;
RA   Takeuchi F., Watanabe S., Baba T., Yuzawa H., Ito T., Morimoto Y.,
RA   Kuroda M., Cui L., Takahashi M., Ankai A., Baba S., Fukui S.,
RA   Lee J.C., Hiramatsu K.;
RT   "Whole-genome sequencing of Staphylococcus haemolyticus uncovers the
RT   extreme plasticity of its genome and the evolution of human-colonizing
RT   staphylococcal species.";
RL   J. Bacteriol. 187:7292-7308(2005).
CC   -!- CATALYTIC ACTIVITY: ATP + pyruvate = ADP + phosphoenolpyruvate.
CC   -!- COFACTOR: Magnesium (By similarity).
CC   -!- COFACTOR: Potassium (By similarity).
CC   -!- PATHWAY: Carbohydrate degradation; glycolysis; pyruvate from D-
CC       glyceraldehyde 3-phosphate: step 5/5.
CC   -!- SIMILARITY: Belongs to the pyruvate kinase family.
CC   -!- SIMILARITY: In the C-terminal section; belongs to the PEP-
CC       utilizing enzyme family.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; AP006716; BAE04536.1; -; Genomic_DNA.
DR   RefSeq; YP_253142.1; -.
DR   GeneID; 3481929; -.
DR   GenomeReviews; AP006716_GR; SH1227.
DR   KEGG; sha:SH1227; -.
DR   NMPDR; fig|279808.3.peg.2549; -.
DR   HOGENOM; Q4L739; -.
DR   OMA; Q4L739; ATESGYT.
DR   BioCyc; SHAE279808:SH1227-MON; -.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0000287; F:magnesium ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0030955; F:potassium ion binding; IEA:InterPro.
DR   GO; GO:0004743; F:pyruvate kinase activity; IEA:EC.
DR   GO; GO:0006096; P:glycolysis; IEA:UniProtKB-KW.
DR   GO; GO:0016310; P:phosphorylation; IEA:InterPro.
DR   InterPro; IPR008279; PEP_mobile.
DR   InterPro; IPR001697; Pyr_Knase.
DR   InterPro; IPR015813; Pyrv/PenolPyrv_Kinase_cat.
DR   InterPro; IPR015794; Pyrv_Knase_a/b.
DR   InterPro; IPR018209; Pyrv_Knase_AS.
DR   InterPro; IPR015793; Pyrv_Knase_brl.
DR   Gene3D; G3DSA:3.50.30.10; PEP_mobile; 1.
DR   Gene3D; G3DSA:3.20.20.60; Pyrv/PenolPyrv_Kinase_cat; 1.
DR   Gene3D; G3DSA:3.40.1380.20; Pyrv_Knase_a/b; 1.
DR   PANTHER; PTHR11817; Pyruvate_kinase; 1.
DR   Pfam; PF00391; PEP-utilizers; 1.
DR   Pfam; PF00224; PK; 1.
DR   Pfam; PF02887; PK_C; 1.
DR   PRINTS; PR01050; PYRUVTKNASE.
DR   ProDom; PD001009; Pyruvate_kinase; 2.
DR   TIGRFAMs; TIGR01064; pyruv_kin; 1.
DR   PROSITE; PS00110; PYRUVATE_KINASE; FALSE_NEG.
PE   3: Inferred from homology;
KW   ATP-binding; Complete proteome; Glycolysis; Kinase; Magnesium;
KW   Metal-binding; Nucleotide-binding; Pyruvate; Transferase.
FT   CHAIN         1    586       Pyruvate kinase.
FT                                /FTId=PRO_0000294139.
FT   ACT_SITE    220    220       By similarity.
FT   METAL       222    222       Magnesium (By similarity).
FT   METAL       243    243       Magnesium (By similarity).
FT   METAL       244    244       Magnesium (By similarity).
SQ   SEQUENCE   586 AA;  63019 MW;  E6C0B61B47DCE8CE CRC64;
     MRKTKIVCTI GPASESEEML EKLMKAGMNV ARLNFSHGSH EEHKARIDTI RKVADRLGKT
     IGILLDTKGP EIRTHDMKDG LIMLEKGKEV IVSMSQVEGT PEKFSVTYED LINDVQVGSY
     ILLDDGLVEL QVKDIDKTKG EVKCDILNTG ELKNKKGVNL PGVKVNLPGI TDKDADDILF
     GIKEDVDYIA ASFVRRPSDV LDIREILERE NNHNITIFPK IENQEGIDNI EEILEVSDGL
     MVARGDMGVE IPPESVPIVQ KDLIRKCNKL GKPVITATQM LDSMQRNPRA TRAEASDVAN
     AIYDGTDAVM LSGETAAGLY PEEAVKTMRN IAVSAEAAQD YKKLLSDRTK LVETSLVNAI
     GVSVAHTALN LNVKAIVAAT ESGSTAVTIS KYRPHSDIIA VTPSEHTARQ LALVWGAYPV
     IKKGRKTTDD LLNNAVATAV ETGRVTNGDL IIITAGVPTG EKGTTNMMKL HLVGDEIAKG
     QGVGRGSVVG KTVVANSASD LEGVDLSESV IVTNSVDETL VPYIEQAVGL ITEENGITSP
     SAIIGLEKSI PTIIGVENAT KELKDGILVT VDAAQGKIFE GYANVL
//