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Protein

Pyruvate kinase

Gene

pyk

Organism
Staphylococcus haemolyticus (strain JCSC1435)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarity
  • K+By similarity

Pathwayi: glycolysis

This protein is involved in step 5 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA), 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI)
  4. Enolase (eno)
  5. Pyruvate kinase (pyk)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei32SubstrateBy similarity1
Metal bindingi34PotassiumBy similarity1
Metal bindingi36PotassiumBy similarity1
Metal bindingi66PotassiumBy similarity1
Metal bindingi67Potassium; via carbonyl oxygenBy similarity1
Sitei220Transition state stabilizerBy similarity1
Metal bindingi222MagnesiumBy similarity1
Binding sitei245Substrate; via amide nitrogenBy similarity1
Metal bindingi246MagnesiumBy similarity1
Binding sitei246Substrate; via amide nitrogenBy similarity1
Binding sitei278SubstrateBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase (EC:2.7.1.40)
Short name:
PK
Gene namesi
Name:pyk
Ordered Locus Names:SH1227
OrganismiStaphylococcus haemolyticus (strain JCSC1435)
Taxonomic identifieri279808 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus
Proteomesi
  • UP000000543 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002941391 – 586Pyruvate kinaseAdd BLAST586

Interactioni

Protein-protein interaction databases

STRINGi279808.SH1227.

Structurei

3D structure databases

ProteinModelPortaliQ4L739.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated
In the C-terminal section; belongs to the PEP-utilizing enzyme family.Curated

Phylogenomic databases

eggNOGiENOG4105CA9. Bacteria.
COG0469. LUCA.
COG3848. LUCA.
HOGENOMiHOG000021559.
KOiK00873.
OMAiGTHEEHK.
OrthoDBiPOG091H02AT.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
3.50.30.10. 1 hit.
InterProiIPR008279. PEP-util_enz_mobile_dom.
IPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00391. PEP-utilizers. 1 hit.
PF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52009. SSF52009. 1 hit.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.

Sequencei

Sequence statusi: Complete.

Q4L739-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRKTKIVCTI GPASESEEML EKLMKAGMNV ARLNFSHGSH EEHKARIDTI
60 70 80 90 100
RKVADRLGKT IGILLDTKGP EIRTHDMKDG LIMLEKGKEV IVSMSQVEGT
110 120 130 140 150
PEKFSVTYED LINDVQVGSY ILLDDGLVEL QVKDIDKTKG EVKCDILNTG
160 170 180 190 200
ELKNKKGVNL PGVKVNLPGI TDKDADDILF GIKEDVDYIA ASFVRRPSDV
210 220 230 240 250
LDIREILERE NNHNITIFPK IENQEGIDNI EEILEVSDGL MVARGDMGVE
260 270 280 290 300
IPPESVPIVQ KDLIRKCNKL GKPVITATQM LDSMQRNPRA TRAEASDVAN
310 320 330 340 350
AIYDGTDAVM LSGETAAGLY PEEAVKTMRN IAVSAEAAQD YKKLLSDRTK
360 370 380 390 400
LVETSLVNAI GVSVAHTALN LNVKAIVAAT ESGSTAVTIS KYRPHSDIIA
410 420 430 440 450
VTPSEHTARQ LALVWGAYPV IKKGRKTTDD LLNNAVATAV ETGRVTNGDL
460 470 480 490 500
IIITAGVPTG EKGTTNMMKL HLVGDEIAKG QGVGRGSVVG KTVVANSASD
510 520 530 540 550
LEGVDLSESV IVTNSVDETL VPYIEQAVGL ITEENGITSP SAIIGLEKSI
560 570 580
PTIIGVENAT KELKDGILVT VDAAQGKIFE GYANVL
Length:586
Mass (Da):63,019
Last modified:August 2, 2005 - v1
Checksum:iE6C0B61B47DCE8CE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006716 Genomic DNA. Translation: BAE04536.1.
RefSeqiWP_011275526.1. NC_007168.1.

Genome annotation databases

EnsemblBacteriaiBAE04536; BAE04536; SH1227.
GeneIDi24247081.
KEGGisha:SH1227.
PATRICi19618573. VBIStaHae67511_1210.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AP006716 Genomic DNA. Translation: BAE04536.1.
RefSeqiWP_011275526.1. NC_007168.1.

3D structure databases

ProteinModelPortaliQ4L739.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi279808.SH1227.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAE04536; BAE04536; SH1227.
GeneIDi24247081.
KEGGisha:SH1227.
PATRICi19618573. VBIStaHae67511_1210.

Phylogenomic databases

eggNOGiENOG4105CA9. Bacteria.
COG0469. LUCA.
COG3848. LUCA.
HOGENOMiHOG000021559.
KOiK00873.
OMAiGTHEEHK.
OrthoDBiPOG091H02AT.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
3.50.30.10. 1 hit.
InterProiIPR008279. PEP-util_enz_mobile_dom.
IPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00391. PEP-utilizers. 1 hit.
PF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52009. SSF52009. 1 hit.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiKPYK_STAHJ
AccessioniPrimary (citable) accession number: Q4L739
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: August 2, 2005
Last modified: November 2, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.