Reviewed, UniProtKB/Swiss-Prot Q4L739 (KPYK_STAHJ)

Names and origin

Protein names

Recommended name:
    Pyruvate kinase
      Short name=PK
    EC=2.7.1.40

Gene names

Name:	pyk
Ordered Locus Names:	SH1227

Organism

Staphylococcus haemolyticus (strain JCSC1435) [Complete proteome] [HAMAP]

Taxonomic identifier

279808 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	586 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + pyruvate = ADP + phosphoenolpyruvate.
Cofactor	Magnesium By similarity. Potassium By similarity.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
Sequence similarities	Belongs to the pyruvate kinase family. In the C-terminal section; belongs to the PEP-utilizing enzyme family.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding Pyruvate
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW phosphorylation Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW potassium ion binding Inferred from electronic annotation. Source: InterPro pyruvate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 586	586	Pyruvate kinase		PRO_0000294139
Sites
	Active site	220	1	By similarity
	Metal binding	222	1	Magnesium By similarity
	Metal binding	243	1	Magnesium By similarity
	Metal binding	244	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q4L739-1 [UniParc]. Last modified August 2, 2005. Version 1. Checksum: E6C0B61B47DCE8CE	FASTA	586	63,019
	10 20 30 40 50 60 MRKTKIVCTI GPASESEEML EKLMKAGMNV ARLNFSHGSH EEHKARIDTI RKVADRLGKT 70 80 90 100 110 120 IGILLDTKGP EIRTHDMKDG LIMLEKGKEV IVSMSQVEGT PEKFSVTYED LINDVQVGSY 130 140 150 160 170 180 ILLDDGLVEL QVKDIDKTKG EVKCDILNTG ELKNKKGVNL PGVKVNLPGI TDKDADDILF 190 200 210 220 230 240 GIKEDVDYIA ASFVRRPSDV LDIREILERE NNHNITIFPK IENQEGIDNI EEILEVSDGL 250 260 270 280 290 300 MVARGDMGVE IPPESVPIVQ KDLIRKCNKL GKPVITATQM LDSMQRNPRA TRAEASDVAN 310 320 330 340 350 360 AIYDGTDAVM LSGETAAGLY PEEAVKTMRN IAVSAEAAQD YKKLLSDRTK LVETSLVNAI 370 380 390 400 410 420 GVSVAHTALN LNVKAIVAAT ESGSTAVTIS KYRPHSDIIA VTPSEHTARQ LALVWGAYPV 430 440 450 460 470 480 IKKGRKTTDD LLNNAVATAV ETGRVTNGDL IIITAGVPTG EKGTTNMMKL HLVGDEIAKG 490 500 510 520 530 540 QGVGRGSVVG KTVVANSASD LEGVDLSESV IVTNSVDETL VPYIEQAVGL ITEENGITSP 550 560 570 580 SAIIGLEKSI PTIIGVENAT KELKDGILVT VDAAQGKIFE GYANVL

References

[1]

"Whole-genome sequencing of Staphylococcus haemolyticus uncovers the extreme plasticity of its genome and the evolution of human-colonizing staphylococcal species." Takeuchi F., Watanabe S., Baba T., Yuzawa H., Ito T., Morimoto Y., Kuroda M., Cui L., Takahashi M., Ankai A., Baba S., Fukui S., Lee J.C., Hiramatsu K. J. Bacteriol. 187:7292-7308(2005) [PubMed: 16237012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AP006716 Genomic DNA. Translation: BAE04536.1.
RefSeq	YP_253142.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3481929.
GenomeReviews	Gene locus SH1227 in contig AP006716_GR.
KEGG	sha:SH1227.
NMPDR	fig|279808.3.peg.2549.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q4L739.
OMA	Q4L739. ATESGYT.
Enzyme and pathway databases
BioCyc	SHAE279808:SH1227-MON.
Family and domain databases
InterPro	IPR008279. PEP_mobile. IPR001697. Pyr_Knase. IPR015813. Pyrv/PenolPyrv_Kinase_cat. IPR015794. Pyrv_Knase_a/b. IPR018209. Pyrv_Knase_AS. IPR015793. Pyrv_Knase_brl. [Graphical view]
Gene3D	G3DSA:3.50.30.10. PEP_mobile. 1 hit. G3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit. G3DSA:3.40.1380.20. Pyrv_Knase_a/b. 1 hit.
PANTHER	PTHR11817. Pyruvate_kinase. 1 hit.
Pfam	PF00391. PEP-utilizers. 1 hit. PF00224. PK. 1 hit. PF02887. PK_C. 1 hit. [Graphical view]
PRINTS	PR01050. PYRUVTKNASE.
ProDom	PD001009. Pyruvate_kinase. 2 hits. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01064. pyruv_kin. 1 hit.
PROSITE	PS00110. PYRUVATE_KINASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPYK_STAHJ

Accession

Primary (citable) accession number: Q4L739

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 10, 2007
Last sequence update:	August 2, 2005
Last modified:	June 16, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)